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The map label for this gene is mltB [H]

Identifier: 59712309

GI number: 59712309

Start: 1919668

End: 1920645

Strand: Reverse

Name: mltB [H]

Synonym: VF_1702

Alternate gene names: 59712309

Gene position: 1920645-1919668 (Counterclockwise)

Preceding gene: 59712310

Following gene: 59712307

Centisome position: 66.29

GC content: 39.06

Gene sequence:

>978_bases
ATGAAGAAAAAATTACTGCTAGGGATAGTCAGTTTATTTTTGGCAACATCAGCATCAGCATCAGCAACACCTGAAGGCGA
CTTTCAAGATTACATTGTTGGGTTGAAAAATGAAGCACGGGAAATTGGGATTTCAGAACCGATTTTAGAACGTGCATTTA
AAAACGTTGCATTTAAAGAGAAAGCAGTAAAATCAGATCGCAATCAACCAGAGAAGAAGTTAACTCTGGATGAATATATT
CCACGAGCTGTGCCAAAATGGAAAATTAAGCAAGCGAACGATTTATATAAAAAACACTACAAAGTGTTAAATCGAATTGG
TGAAGAGTATGGCGTTCAACCTCGATTTATTGTTGCATTATGGGGTGTTGAAAGTAACTTTGGTAAATTAACGGGTGGTT
ATAACGTTATTGAAGCATTAACGACGTTGGCTTATGACGGTCGTCGTGAAGCGTTCTTCAAAAAACAAACCATGGCTGCA
TTGACCATTTTACAGCAAGGCCACATTACACCTGAAAACATGAAAGGCTCATGGGCTGGTGCAATGGGTCAATGCCAATT
TATGCCAACATCATTTTTATCTTATGCTGTTGATGGAAATGGTGATGGAAAAAAAGACATTTGGAATACACATGCCGATG
TATTTGCTTCCGCCGCTAATTACTTAAAACAAGTAGGTTGGGACGATACTTATACATGGGGACGTCAAGTGCAATTGCCT
GAATCTTTGGATGCAGAAAGCCTAAAAGGGTTGTCTGAAGAAAAAGGTAAGACGTTAGCACAATGGCAAGAGCTAGGTGT
TCGTCGTTTAACAGGTAAAGCATTACCTGATGCAGATATTAAAGCATGGCTAGTTCAACCGGATGATAAAGATGGTCGAG
CGTACCTTGTATATAACAACTACCAAGTATTAATGGATTGGAACCGTTCGCATTATTTTGCGTTAGCGGTAAGTCACTTG
GCTGATTCGATTAAGTAA

Upstream 100 bases:

>100_bases
TAAAAGAAAATGGATTCTTTTTGCAGTTACCACCACCACCTGAAAACCTGCTTGAAAAATACAAAAAAAGATAAAGCAGC
AAGAGAAGAGAATTAATTAT

Downstream 100 bases:

>100_bases
CAGAGCAGGGTTCAGATCGCTTCGCTTAAAGGATTCAGGGGAGTTTGTTGTGAGTTCATCGCTTACAACTAGCCCCCTGA
AACCAGAAAGGGAGCTAGCG

Product: membrane-bound lytic murein transglycosylase B

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MKKKLLLGIVSLFLATSASASATPEGDFQDYIVGLKNEAREIGISEPILERAFKNVAFKEKAVKSDRNQPEKKLTLDEYI
PRAVPKWKIKQANDLYKKHYKVLNRIGEEYGVQPRFIVALWGVESNFGKLTGGYNVIEALTTLAYDGRREAFFKKQTMAA
LTILQQGHITPENMKGSWAGAMGQCQFMPTSFLSYAVDGNGDGKKDIWNTHADVFASAANYLKQVGWDDTYTWGRQVQLP
ESLDAESLKGLSEEKGKTLAQWQELGVRRLTGKALPDADIKAWLVQPDDKDGRAYLVYNNYQVLMDWNRSHYFALAVSHL
ADSIK

Sequences:

>Translated_325_residues
MKKKLLLGIVSLFLATSASASATPEGDFQDYIVGLKNEAREIGISEPILERAFKNVAFKEKAVKSDRNQPEKKLTLDEYI
PRAVPKWKIKQANDLYKKHYKVLNRIGEEYGVQPRFIVALWGVESNFGKLTGGYNVIEALTTLAYDGRREAFFKKQTMAA
LTILQQGHITPENMKGSWAGAMGQCQFMPTSFLSYAVDGNGDGKKDIWNTHADVFASAANYLKQVGWDDTYTWGRQVQLP
ESLDAESLKGLSEEKGKTLAQWQELGVRRLTGKALPDADIKAWLVQPDDKDGRAYLVYNNYQVLMDWNRSHYFALAVSHL
ADSIK
>Mature_325_residues
MKKKLLLGIVSLFLATSASASATPEGDFQDYIVGLKNEAREIGISEPILERAFKNVAFKEKAVKSDRNQPEKKLTLDEYI
PRAVPKWKIKQANDLYKKHYKVLNRIGEEYGVQPRFIVALWGVESNFGKLTGGYNVIEALTTLAYDGRREAFFKKQTMAA
LTILQQGHITPENMKGSWAGAMGQCQFMPTSFLSYAVDGNGDGKKDIWNTHADVFASAANYLKQVGWDDTYTWGRQVQLP
ESLDAESLKGLSEEKGKTLAQWQELGVRRLTGKALPDADIKAWLVQPDDKDGRAYLVYNNYQVLMDWNRSHYFALAVSHL
ADSIK

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=232, Percent_Identity=32.3275862068966, Blast_Score=106, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 36567; Mature: 36567

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKLLLGIVSLFLATSASASATPEGDFQDYIVGLKNEAREIGISEPILERAFKNVAFKE
CCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
KAVKSDRNQPEKKLTLDEYIPRAVPKWKIKQANDLYKKHYKVLNRIGEEYGVQPRFIVAL
HHHHHCCCCCHHHCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
WGVESNFGKLTGGYNVIEALTTLAYDGRREAFFKKQTMAALTILQQGHITPENMKGSWAG
ECCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
AMGQCQFMPTSFLSYAVDGNGDGKKDIWNTHADVFASAANYLKQVGWDDTYTWGRQVQLP
CCCCEEECCHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECC
ESLDAESLKGLSEEKGKTLAQWQELGVRRLTGKALPDADIKAWLVQPDDKDGRAYLVYNN
CCCCHHHHCCCHHHHCHHHHHHHHCCHHHHCCCCCCCCCCEEEEECCCCCCCCEEEEECC
YQVLMDWNRSHYFALAVSHLADSIK
EEEEEECCCCEEHHHHHHHHHHHCC
>Mature Secondary Structure
MKKKLLLGIVSLFLATSASASATPEGDFQDYIVGLKNEAREIGISEPILERAFKNVAFKE
CCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
KAVKSDRNQPEKKLTLDEYIPRAVPKWKIKQANDLYKKHYKVLNRIGEEYGVQPRFIVAL
HHHHHCCCCCHHHCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
WGVESNFGKLTGGYNVIEALTTLAYDGRREAFFKKQTMAALTILQQGHITPENMKGSWAG
ECCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
AMGQCQFMPTSFLSYAVDGNGDGKKDIWNTHADVFASAANYLKQVGWDDTYTWGRQVQLP
CCCCEEECCHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECC
ESLDAESLKGLSEEKGKTLAQWQELGVRRLTGKALPDADIKAWLVQPDDKDGRAYLVYNN
CCCCHHHHCCCHHHHCHHHHHHHHCCHHHHCCCCCCCCCCEEEEECCCCCCCCEEEEECC
YQVLMDWNRSHYFALAVSHLADSIK
EEEEEECCCCEEHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]