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The map label for this gene is menF [H]

Identifier: 59712279

GI number: 59712279

Start: 1881572

End: 1882894

Strand: Reverse

Name: menF [H]

Synonym: VF_1672

Alternate gene names: 59712279

Gene position: 1882894-1881572 (Counterclockwise)

Preceding gene: 59712281

Following gene: 59712278

Centisome position: 64.98

GC content: 39.53

Gene sequence:

>1323_bases
TTGTCCACTTTTCAAGATGCCATTGAATCGATCCGTCATCAAATTACACAGGCAACAACATCCCATACAATTTCTTTAAA
ACTGGATTGGTCTATTGGTGAGCAGCTAATTGATTGGTTAGAAGCACAACCTGTATTTCCTAAGTTTTATTGGCAATCAC
GTGACGGTGAAGAAGAAGTGGCAGTATTGGGTCAAGTTAAGACGTTTACCGATCCTAGTGCCGCTCAAAAAGTTCTCGCA
CCTCAACAACGTATTTGGGGGGGCCGATCTTTTGATGGTCGAACTGAACGTAATCGTCGCTGTATGTCATCTTTCTTTTT
TCTTCCTCAAGTAGAAATTACGCGTTTGGGTTCATCGTGGAATATTATGGTGAATGTGTCAGAAGATAATTCTCGCTTGC
TCTCTACATTAAATAAAATGACAGAAGAGTTTGACGATTTATCTGAATTACATTGCCGAGTAACTCAACGTATCAATACG
CCTGAAAAAGCGGATTGGTCAAACATGGTCAATAAAGCGTTAACTGCAATTGAAAATAAGCAGTTTGAAAAAGTGGTACT
CGCTCGTAAAACGACATTAACGCTTGATCTGAATTTATCGTCTGCTCAGTTTTTAAAAGCAAGTCGCCAGTCAAATAGCC
ACAGTTTTCATTTTATGATGGCGTTGGATAGCGAGCATTGTTTTATTGGTTCAACACCAGAGCGTTTATATGTACGTAAT
GGCTATGCTTTAAAAACAGAAGCATTAGCCGGTACGATTGGACGTGGCCATGACGAAGAAGAAGATAATCAATTAGCTTC
ATGGTTAATTTCGGATAAGAAAAATCAGTATGAAAACCGTTTAGTGGTGGACGATATTGTTCACCGTTTATCTAAGTTCA
GTATTTCAATGAACGTTGCTGATACATCTGAATTGGTGAAATTACGCAAAGTTCAGCATTTAAAACGTCCTATTTCTGCT
GAAATTAATGCGGAATGCAGTGACTCTCAATTACTTGATAACTTACAACCAACGGCAGCTATTGCTGGATTACCTCGTAT
GTCGGCTTTGGATTTCATTATTGATAATGAACCTTTTGCTCGTGGTTGGTATAGCGGTGCTGTCGGCTTCTTGAGTCAAC
AGCGCAGTGAATTTTGTGTTGCTATTCGTAGTGCATTGGTTATGGGTAATAAACTTCACTTATTTGCAGGTGCTGGTATT
GTTCCGGGTTCTGAGCCAAGCAGTGAATGGGACGAGTTAGATAGAAAAACCTCTACTTTATGTACCTTATTAGAATCTGA
TACTAATGTGAGTTCAGATATCACGGAGTCGCAAGTCGCATGA

Upstream 100 bases:

>100_bases
CCTTTTGCTAGAATCACTAATTCATACAAGGAAATAACAAGCAATTCCGCTTGTGCAAGTCGGTTGCCATGATGGCTGAA
AACGATCAAGAGAGGTTAAT

Downstream 100 bases:

>100_bases
TGCAACCTGAGCAGCAAGTAATGCTAAATCAAGTTTGGGCTGAGTTAATTATTGAGGAGTTAGTTCGAAATGGAGTAAAG
CATATTTGCATCGCTCCGGG

Product: isochorismate synthase 2

Products: NA

Alternate protein names: Isochorismate mutase [H]

Number of amino acids: Translated: 440; Mature: 439

Protein sequence:

>440_residues
MSTFQDAIESIRHQITQATTSHTISLKLDWSIGEQLIDWLEAQPVFPKFYWQSRDGEEEVAVLGQVKTFTDPSAAQKVLA
PQQRIWGGRSFDGRTERNRRCMSSFFFLPQVEITRLGSSWNIMVNVSEDNSRLLSTLNKMTEEFDDLSELHCRVTQRINT
PEKADWSNMVNKALTAIENKQFEKVVLARKTTLTLDLNLSSAQFLKASRQSNSHSFHFMMALDSEHCFIGSTPERLYVRN
GYALKTEALAGTIGRGHDEEEDNQLASWLISDKKNQYENRLVVDDIVHRLSKFSISMNVADTSELVKLRKVQHLKRPISA
EINAECSDSQLLDNLQPTAAIAGLPRMSALDFIIDNEPFARGWYSGAVGFLSQQRSEFCVAIRSALVMGNKLHLFAGAGI
VPGSEPSSEWDELDRKTSTLCTLLESDTNVSSDITESQVA

Sequences:

>Translated_440_residues
MSTFQDAIESIRHQITQATTSHTISLKLDWSIGEQLIDWLEAQPVFPKFYWQSRDGEEEVAVLGQVKTFTDPSAAQKVLA
PQQRIWGGRSFDGRTERNRRCMSSFFFLPQVEITRLGSSWNIMVNVSEDNSRLLSTLNKMTEEFDDLSELHCRVTQRINT
PEKADWSNMVNKALTAIENKQFEKVVLARKTTLTLDLNLSSAQFLKASRQSNSHSFHFMMALDSEHCFIGSTPERLYVRN
GYALKTEALAGTIGRGHDEEEDNQLASWLISDKKNQYENRLVVDDIVHRLSKFSISMNVADTSELVKLRKVQHLKRPISA
EINAECSDSQLLDNLQPTAAIAGLPRMSALDFIIDNEPFARGWYSGAVGFLSQQRSEFCVAIRSALVMGNKLHLFAGAGI
VPGSEPSSEWDELDRKTSTLCTLLESDTNVSSDITESQVA
>Mature_439_residues
STFQDAIESIRHQITQATTSHTISLKLDWSIGEQLIDWLEAQPVFPKFYWQSRDGEEEVAVLGQVKTFTDPSAAQKVLAP
QQRIWGGRSFDGRTERNRRCMSSFFFLPQVEITRLGSSWNIMVNVSEDNSRLLSTLNKMTEEFDDLSELHCRVTQRINTP
EKADWSNMVNKALTAIENKQFEKVVLARKTTLTLDLNLSSAQFLKASRQSNSHSFHFMMALDSEHCFIGSTPERLYVRNG
YALKTEALAGTIGRGHDEEEDNQLASWLISDKKNQYENRLVVDDIVHRLSKFSISMNVADTSELVKLRKVQHLKRPISAE
INAECSDSQLLDNLQPTAAIAGLPRMSALDFIIDNEPFARGWYSGAVGFLSQQRSEFCVAIRSALVMGNKLHLFAGAGIV
PGSEPSSEWDELDRKTSTLCTLLESDTNVSSDITESQVA

Specific function: Menaquinone biosynthesis. [C]

COG id: COG1169

COG function: function code HQ; Isochorismate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismate synthase family [H]

Homologues:

Organism=Escherichia coli, GI87082077, Length=401, Percent_Identity=41.645885286783, Blast_Score=293, Evalue=1e-80,
Organism=Escherichia coli, GI1786809, Length=272, Percent_Identity=31.6176470588235, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1788114, Length=224, Percent_Identity=26.3392857142857, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1787518, Length=309, Percent_Identity=25.2427184466019, Blast_Score=83, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6320935, Length=210, Percent_Identity=29.0476190476191, Blast_Score=76, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR015890
- InterPro:   IPR004561 [H]

Pfam domain/function: PF00425 Chorismate_bind [H]

EC number: =5.4.4.2 [H]

Molecular weight: Translated: 49521; Mature: 49390

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTFQDAIESIRHQITQATTSHTISLKLDWSIGEQLIDWLEAQPVFPKFYWQSRDGEEEV
CCHHHHHHHHHHHHHHHHCCCEEEEEEEEHHHHHHHHHHHHCCCCCCHHHCCCCCCHHHH
AVLGQVKTFTDPSAAQKVLAPQQRIWGGRSFDGRTERNRRCMSSFFFLPQVEITRLGSSW
HHHHHHHCCCCHHHHHHHHCCHHHHCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCE
NIMVNVSEDNSRLLSTLNKMTEEFDDLSELHCRVTQRINTPEKADWSNMVNKALTAIENK
EEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCH
QFEKVVLARKTTLTLDLNLSSAQFLKASRQSNSHSFHFMMALDSEHCFIGSTPERLYVRN
HHHHHHHEEEEEEEEEECCCHHHHHHHHHCCCCCCEEEEEEECCCCEEECCCCCEEEEEC
GYALKTEALAGTIGRGHDEEEDNQLASWLISDKKNQYENRLVVDDIVHRLSKFSISMNVA
CCEEEHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHEEEEECC
DTSELVKLRKVQHLKRPISAEINAECSDSQLLDNLQPTAAIAGLPRMSALDFIIDNEPFA
CHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCHHHHHCCCCHHHHHEEECCCCHH
RGWYSGAVGFLSQQRSEFCVAIRSALVMGNKLHLFAGAGIVPGSEPSSEWDELDRKTSTL
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHH
CTLLESDTNVSSDITESQVA
HHHHHCCCCCCCCCHHHCCC
>Mature Secondary Structure 
STFQDAIESIRHQITQATTSHTISLKLDWSIGEQLIDWLEAQPVFPKFYWQSRDGEEEV
CHHHHHHHHHHHHHHHHCCCEEEEEEEEHHHHHHHHHHHHCCCCCCHHHCCCCCCHHHH
AVLGQVKTFTDPSAAQKVLAPQQRIWGGRSFDGRTERNRRCMSSFFFLPQVEITRLGSSW
HHHHHHHCCCCHHHHHHHHCCHHHHCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCE
NIMVNVSEDNSRLLSTLNKMTEEFDDLSELHCRVTQRINTPEKADWSNMVNKALTAIENK
EEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCH
QFEKVVLARKTTLTLDLNLSSAQFLKASRQSNSHSFHFMMALDSEHCFIGSTPERLYVRN
HHHHHHHEEEEEEEEEECCCHHHHHHHHHCCCCCCEEEEEEECCCCEEECCCCCEEEEEC
GYALKTEALAGTIGRGHDEEEDNQLASWLISDKKNQYENRLVVDDIVHRLSKFSISMNVA
CCEEEHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHEEEEECC
DTSELVKLRKVQHLKRPISAEINAECSDSQLLDNLQPTAAIAGLPRMSALDFIIDNEPFA
CHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCHHHHHCCCCHHHHHEEECCCCHH
RGWYSGAVGFLSQQRSEFCVAIRSALVMGNKLHLFAGAGIVPGSEPSSEWDELDRKTSTL
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHH
CTLLESDTNVSSDITESQVA
HHHHHCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8549818; 8764478; 9205837; 9278503; 2666397; 7984428 [H]