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The map label for this gene is dusC [H]

Identifier: 59712245

GI number: 59712245

Start: 1840761

End: 1841708

Strand: Direct

Name: dusC [H]

Synonym: VF_1638

Alternate gene names: 59712245

Gene position: 1840761-1841708 (Clockwise)

Preceding gene: 59712244

Following gene: 59712250

Centisome position: 63.53

GC content: 40.3

Gene sequence:

>948_bases
ATGCAAGTAATACTAGGCCCTATGGAGGGCGTACTCGATCATTTAATGCGCGAGTTACTAACTGAAATTAATGATTACGA
TTTCTGTGTTACAGAGTTTGTTCGTGTTGTCGATCAACTGCTTCCCCCTCATGTATTCCATCGAATTTGTCCTGAGCTAC
TAAATAACAGTAAAACTAAATCAGGTACCCCTATTCGTCTTCAACTATTAGGTCAAGAGCCTAACTGGATGGCTGAGAAT
GCTGCGAGAGCAATTGAACTTGGCTCTGACGGTATTGATCTGAATTTTGGCTGCCCAGCTAAAGCGGTAAACAAAAGTCG
TGGCGGAGCTGCGCTTTTAAAAGAGCCAGAGCTCATCTACCAAGTTGTTAAAGCTTGTCGAGATGCCGTAAGTAAAGATA
AGAAAGTTACGGCTAAAATTCGTTTAGGCTGGGAAAACCCTGAAGAATGCTTTGTTATTGCCGATGCCATTAAACAAGCT
GGAGCGGATGAACTAACCATTCATGCAAGAACCAAAGTAGATGGGTATAAAGCAGAAGAAATTAAATGGGATTACATTAA
CCAAGTTCGCCAAAAGATTGATATTCCAATTATCGCAAATGGTGAAATCTGGAACTATGATGACGGCCAACGCTGTATTG
CAGCAACAGGAATCGATTCATTAATGGTATGCCGTGGGGCTTTTAATGTGCCCAACTTAGGTAACGTAGTTAAGCACAAC
CATTCAAAAATGCCTTGGCCTGAAGTCATTGCTTTGCTTATTCATTATTCGAAGCTTCAAATGGCAGGAGATAAAGGACA
ATATTACTCAAATCGAGTAAAGCAGTGGCTTGTTTATCTTCGTCAAGAATATACCGAAGCAAAAGATATTTTTATGGATA
TTCGAAGTCATAAAAAATCAGAGCCTATCGTTGCCCGTCTAAATCAAGAGCTAGAAAAAGTAAGCTAG

Upstream 100 bases:

>100_bases
ATCGACGGGAGAAACGATAATTACTACTTATAACAAAGGAAGTAAGTGACATAGGCTGCCTTTCCCCCTACAATCCCACC
TCCAATAAAAGAGGTGGGAT

Downstream 100 bases:

>100_bases
CCTTATTTTTTCGCACATATTTCCCCGAACCAATGGCCGAGTTTATGAAAAGTAAACTCGGTTTTTACCATCTTGTTTCG
CCTTATAAAGCAAGGCATCG

Product: tRNA-dihydrouridine synthase C

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MQVILGPMEGVLDHLMRELLTEINDYDFCVTEFVRVVDQLLPPHVFHRICPELLNNSKTKSGTPIRLQLLGQEPNWMAEN
AARAIELGSDGIDLNFGCPAKAVNKSRGGAALLKEPELIYQVVKACRDAVSKDKKVTAKIRLGWENPEECFVIADAIKQA
GADELTIHARTKVDGYKAEEIKWDYINQVRQKIDIPIIANGEIWNYDDGQRCIAATGIDSLMVCRGAFNVPNLGNVVKHN
HSKMPWPEVIALLIHYSKLQMAGDKGQYYSNRVKQWLVYLRQEYTEAKDIFMDIRSHKKSEPIVARLNQELEKVS

Sequences:

>Translated_315_residues
MQVILGPMEGVLDHLMRELLTEINDYDFCVTEFVRVVDQLLPPHVFHRICPELLNNSKTKSGTPIRLQLLGQEPNWMAEN
AARAIELGSDGIDLNFGCPAKAVNKSRGGAALLKEPELIYQVVKACRDAVSKDKKVTAKIRLGWENPEECFVIADAIKQA
GADELTIHARTKVDGYKAEEIKWDYINQVRQKIDIPIIANGEIWNYDDGQRCIAATGIDSLMVCRGAFNVPNLGNVVKHN
HSKMPWPEVIALLIHYSKLQMAGDKGQYYSNRVKQWLVYLRQEYTEAKDIFMDIRSHKKSEPIVARLNQELEKVS
>Mature_315_residues
MQVILGPMEGVLDHLMRELLTEINDYDFCVTEFVRVVDQLLPPHVFHRICPELLNNSKTKSGTPIRLQLLGQEPNWMAEN
AARAIELGSDGIDLNFGCPAKAVNKSRGGAALLKEPELIYQVVKACRDAVSKDKKVTAKIRLGWENPEECFVIADAIKQA
GADELTIHARTKVDGYKAEEIKWDYINQVRQKIDIPIIANGEIWNYDDGQRCIAATGIDSLMVCRGAFNVPNLGNVVKHN
HSKMPWPEVIALLIHYSKLQMAGDKGQYYSNRVKQWLVYLRQEYTEAKDIFMDIRSHKKSEPIVARLNQELEKVS

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusC subfamily [H]

Homologues:

Organism=Homo sapiens, GI40807366, Length=191, Percent_Identity=29.3193717277487, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI31742496, Length=178, Percent_Identity=26.9662921348315, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI239788483, Length=180, Percent_Identity=30, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI239788462, Length=180, Percent_Identity=30, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI8923374, Length=252, Percent_Identity=27.3809523809524, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1788462, Length=312, Percent_Identity=59.9358974358974, Blast_Score=400, Evalue=1e-113,
Organism=Escherichia coli, GI1789660, Length=241, Percent_Identity=34.4398340248963, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI145693211, Length=182, Percent_Identity=31.8681318681319, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI25144369, Length=187, Percent_Identity=31.0160427807487, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17507177, Length=154, Percent_Identity=33.1168831168831, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323560, Length=201, Percent_Identity=28.8557213930348, Blast_Score=82, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6323437, Length=160, Percent_Identity=29.375, Blast_Score=70, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6323433, Length=164, Percent_Identity=28.6585365853659, Blast_Score=64, Evalue=4e-11,
Organism=Drosophila melanogaster, GI19921524, Length=174, Percent_Identity=32.7586206896552, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24580595, Length=171, Percent_Identity=32.7485380116959, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI19920448, Length=171, Percent_Identity=32.7485380116959, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24585320, Length=184, Percent_Identity=30.4347826086957, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI45549423, Length=159, Percent_Identity=32.7044025157233, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 35684; Mature: 35684

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVILGPMEGVLDHLMRELLTEINDYDFCVTEFVRVVDQLLPPHVFHRICPELLNNSKTK
CCEEECHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCC
SGTPIRLQLLGQEPNWMAENAARAIELGSDGIDLNFGCPAKAVNKSRGGAALLKEPELIY
CCCCEEEEEECCCCCHHHHHHHHHEEECCCCCEECCCCCHHHHCCCCCCCHHHCCHHHHH
QVVKACRDAVSKDKKVTAKIRLGWENPEECFVIADAIKQAGADELTIHARTKVDGYKAEE
HHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHH
IKWDYINQVRQKIDIPIIANGEIWNYDDGQRCIAATGIDSLMVCRGAFNVPNLGNVVKHN
CCHHHHHHHHHHCCCCEEECCCEECCCCCCEEEECCCHHHHHHHHCCCCCCCCCHHHHCC
HSKMPWPEVIALLIHYSKLQMAGDKGQYYSNRVKQWLVYLRQEYTEAKDIFMDIRSHKKS
CCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
EPIVARLNQELEKVS
CHHHHHHHHHHHHCC
>Mature Secondary Structure
MQVILGPMEGVLDHLMRELLTEINDYDFCVTEFVRVVDQLLPPHVFHRICPELLNNSKTK
CCEEECHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCC
SGTPIRLQLLGQEPNWMAENAARAIELGSDGIDLNFGCPAKAVNKSRGGAALLKEPELIY
CCCCEEEEEECCCCCHHHHHHHHHEEECCCCCEECCCCCHHHHCCCCCCCHHHCCHHHHH
QVVKACRDAVSKDKKVTAKIRLGWENPEECFVIADAIKQAGADELTIHARTKVDGYKAEE
HHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHH
IKWDYINQVRQKIDIPIIANGEIWNYDDGQRCIAATGIDSLMVCRGAFNVPNLGNVVKHN
CCHHHHHHHHHHCCCCEEECCCEECCCCCCEEEECCCHHHHHHHHCCCCCCCCCHHHHCC
HSKMPWPEVIALLIHYSKLQMAGDKGQYYSNRVKQWLVYLRQEYTEAKDIFMDIRSHKKS
CCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
EPIVARLNQELEKVS
CHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]