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The map label for this gene is sppA [H]

Identifier: 59712250

GI number: 59712250

Start: 1848495

End: 1850351

Strand: Direct

Name: sppA [H]

Synonym: VF_1643

Alternate gene names: 59712250

Gene position: 1848495-1850351 (Clockwise)

Preceding gene: 59712245

Following gene: 59712251

Centisome position: 63.8

GC content: 39.15

Gene sequence:

>1857_bases
ATGAAAGGGTTATTCCACTTCATAGGCAAATGTTTTCAAGCGATATGGAAGTTACTTTCCTTTACGCGCCAGCTCGTACT
TAATCTGTTTTTTCTCATTTTTATTGGCGTCATTGCTTTCGCATTCTTAGATAGCACTCCCCCATCAACAGAAGATAAAA
TTGTTGAACCAAAAGCATTAATTCTCGACTTATCTGGCCCAATTGTTGAAGAAAGCAAATTTAGAGAGCCATTAGAACGA
ATTACTTCGGATGTTTTAGGTAGCCAAAAATCACAAGAAAATGTGTTATTTGATATAGTCGATACAATTCGTTTCGCAGC
ACATGACAATAATGTGTCTGGTTTGGTGCTGCATTTAAAAGAAATGAATGAAACCAGCCTCACTAAACTGCGCTATATTG
CTAAAGCGATAAATACCTTTAAAGCCGCAGGCAAACCTGTTTATGCAATTGGTGATTATTACAATCAGAGCCAATATTAC
CTTGCAAGCTATGCTGATAAAGTATTCATGGCTCCAGATGGTACTGTTTTGTTGCGTGGCTATGGTGCATATACGCTTTA
CTACAAAGAGTTGCTAGAGAAACTAAACGTATCTACTCATGTATTCCGTGTGGGTACATATAAATCTGCAGTTGAACCCT
ACCTTCGTAATGACATGTCTGATGCTGCAAAAGAATCAGCTTCTGCTTGGTTAACCCAACTTTGGGATGCGTACTTAGAT
GACGTTGCGACCAATCGCCAAATCGATCCAAAAACACTGACAATGCCTATGGATCAATTCATCGCTAAATTAAAATCAGT
GAATGGTGATCTATCGCAAATGATGGTAGAACTGGGTTTAGTCGATAAACTAGCGACACGACAAGAAGTTCGCAAAGATT
TAATTGAACAATTTGGTTCAAATGGTTACGACAGCTTCAAACAAATCAGCTATTACGATTATTTGTCTCAAGTTCAACCA
ACTGTTATTCCTGACGCACAAGATATCGCTGTTGTTGTAGCAAGTGGTGCCATTATGGATGGAACAGAACGCCAAGGTAC
AGTGGGTGGTGATTCTACAGCAGCACTTCTTCGTCAAGCTCGTGGCGACGATAAAGTAAAAGCGGTGGTACTTCGTGTTG
ATAGTCCAGGAGGAAGTGCTTTTGCTTCTGAAGTCATTCGTAATGAAATTGATGCATTAAAAGAAGCAGGAAAACCAGTG
GTTGTCTCTATGTCTAGTGTAGCGGCTTCTGGTGGTTATTGGATTTCAGCAAGTGCTGATAAAATTATTGCTCAGCCAAC
AACAATTACTGGTTCTATTGGTATTTTTGGCATTTTAACGACATTTGAAAAAAGTTTAGAGAAAATGGGGATCCACAGTG
ATGGGATCTCAACCTCTCCTTTCAATGGCGTTAGTATTACTCGTCCTTTAAATGATGATGTTGCTCAAGTTATGCAATTG
GGTATTGAACATGGTTACCATCGTTTCATTAAACTTGTCAGCGATCATCGTAACCTATCATTAGAGGCCGTAGACAAAGT
GGCTCAAGGCCGAGTTTGGACAGGTAAAGATGCGTTAAAACATGGTCTTGTTGATCAGCTGGGTGATTTTGATGATGCTG
TGCAAGCAGCTGCTCAATTAGCAAATATGGAATCATACAACCTATATTGGGTAAAAGAGCCACTAACGCCAATGGAGCAG
TTCTTAGAAGAATTAAGCATGAACCTAAATGCGAGCGTCAGAACTGAGTTATTTTCATTCGCTCCTGAAGCTTTGCAACC
AACAATCAATAAAGTTGCAACAGATATCAACATGTTAAATAACTTTAATGATCCAAAAGGGCAATATCTCTTCTGTTTAA
ACTGTAGCAATCTTTAA

Upstream 100 bases:

>100_bases
ATCGTACTAATTCTTACTTAGACCTTTCGGCCTAGTTACTTTATTTACTACTTAGAGTAGGATATAGACATAGATCATAA
AATGTATAATAAGGAAATAT

Downstream 100 bases:

>100_bases
AAGCATGACGAGCCCGACACATGTCGGGCTTTCTTTATGCCAAATGCACAGTATAATCCCCCCACCTCCCATAACTCTGT
ACCGTTGTTATTATGGAAAG

Product: protease IV (signal peptide peptidase)

Products: NA

Alternate protein names: Endopeptidase IV; Protease IV; Signal peptide peptidase [H]

Number of amino acids: Translated: 618; Mature: 618

Protein sequence:

>618_residues
MKGLFHFIGKCFQAIWKLLSFTRQLVLNLFFLIFIGVIAFAFLDSTPPSTEDKIVEPKALILDLSGPIVEESKFREPLER
ITSDVLGSQKSQENVLFDIVDTIRFAAHDNNVSGLVLHLKEMNETSLTKLRYIAKAINTFKAAGKPVYAIGDYYNQSQYY
LASYADKVFMAPDGTVLLRGYGAYTLYYKELLEKLNVSTHVFRVGTYKSAVEPYLRNDMSDAAKESASAWLTQLWDAYLD
DVATNRQIDPKTLTMPMDQFIAKLKSVNGDLSQMMVELGLVDKLATRQEVRKDLIEQFGSNGYDSFKQISYYDYLSQVQP
TVIPDAQDIAVVVASGAIMDGTERQGTVGGDSTAALLRQARGDDKVKAVVLRVDSPGGSAFASEVIRNEIDALKEAGKPV
VVSMSSVAASGGYWISASADKIIAQPTTITGSIGIFGILTTFEKSLEKMGIHSDGISTSPFNGVSITRPLNDDVAQVMQL
GIEHGYHRFIKLVSDHRNLSLEAVDKVAQGRVWTGKDALKHGLVDQLGDFDDAVQAAAQLANMESYNLYWVKEPLTPMEQ
FLEELSMNLNASVRTELFSFAPEALQPTINKVATDINMLNNFNDPKGQYLFCLNCSNL

Sequences:

>Translated_618_residues
MKGLFHFIGKCFQAIWKLLSFTRQLVLNLFFLIFIGVIAFAFLDSTPPSTEDKIVEPKALILDLSGPIVEESKFREPLER
ITSDVLGSQKSQENVLFDIVDTIRFAAHDNNVSGLVLHLKEMNETSLTKLRYIAKAINTFKAAGKPVYAIGDYYNQSQYY
LASYADKVFMAPDGTVLLRGYGAYTLYYKELLEKLNVSTHVFRVGTYKSAVEPYLRNDMSDAAKESASAWLTQLWDAYLD
DVATNRQIDPKTLTMPMDQFIAKLKSVNGDLSQMMVELGLVDKLATRQEVRKDLIEQFGSNGYDSFKQISYYDYLSQVQP
TVIPDAQDIAVVVASGAIMDGTERQGTVGGDSTAALLRQARGDDKVKAVVLRVDSPGGSAFASEVIRNEIDALKEAGKPV
VVSMSSVAASGGYWISASADKIIAQPTTITGSIGIFGILTTFEKSLEKMGIHSDGISTSPFNGVSITRPLNDDVAQVMQL
GIEHGYHRFIKLVSDHRNLSLEAVDKVAQGRVWTGKDALKHGLVDQLGDFDDAVQAAAQLANMESYNLYWVKEPLTPMEQ
FLEELSMNLNASVRTELFSFAPEALQPTINKVATDINMLNNFNDPKGQYLFCLNCSNL
>Mature_618_residues
MKGLFHFIGKCFQAIWKLLSFTRQLVLNLFFLIFIGVIAFAFLDSTPPSTEDKIVEPKALILDLSGPIVEESKFREPLER
ITSDVLGSQKSQENVLFDIVDTIRFAAHDNNVSGLVLHLKEMNETSLTKLRYIAKAINTFKAAGKPVYAIGDYYNQSQYY
LASYADKVFMAPDGTVLLRGYGAYTLYYKELLEKLNVSTHVFRVGTYKSAVEPYLRNDMSDAAKESASAWLTQLWDAYLD
DVATNRQIDPKTLTMPMDQFIAKLKSVNGDLSQMMVELGLVDKLATRQEVRKDLIEQFGSNGYDSFKQISYYDYLSQVQP
TVIPDAQDIAVVVASGAIMDGTERQGTVGGDSTAALLRQARGDDKVKAVVLRVDSPGGSAFASEVIRNEIDALKEAGKPV
VVSMSSVAASGGYWISASADKIIAQPTTITGSIGIFGILTTFEKSLEKMGIHSDGISTSPFNGVSITRPLNDDVAQVMQL
GIEHGYHRFIKLVSDHRNLSLEAVDKVAQGRVWTGKDALKHGLVDQLGDFDDAVQAAAQLANMESYNLYWVKEPLTPMEQ
FLEELSMNLNASVRTELFSFAPEALQPTINKVATDINMLNNFNDPKGQYLFCLNCSNL

Specific function: Digestion of the cleaved signal peptides. This activity is necessary to maintain proper secretion of mature proteins across the membrane [H]

COG id: COG0616

COG function: function code OU; Periplasmic serine proteases (ClpP class)

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S49 family [H]

Homologues:

Organism=Escherichia coli, GI1788064, Length=627, Percent_Identity=48.8038277511962, Blast_Score=567, Evalue=1e-163,
Organism=Escherichia coli, GI1787527, Length=176, Percent_Identity=28.9772727272727, Blast_Score=77, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004634
- InterPro:   IPR004635
- InterPro:   IPR002142 [H]

Pfam domain/function: PF01343 Peptidase_S49 [H]

EC number: 3.4.21.- [C]

Molecular weight: Translated: 68316; Mature: 68316

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGLFHFIGKCFQAIWKLLSFTRQLVLNLFFLIFIGVIAFAFLDSTPPSTEDKIVEPKAL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEE
ILDLSGPIVEESKFREPLERITSDVLGSQKSQENVLFDIVDTIRFAAHDNNVSGLVLHLK
EEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEH
EMNETSLTKLRYIAKAINTFKAAGKPVYAIGDYYNQSQYYLASYADKVFMAPDGTVLLRG
HCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHCCEEEECCCCEEEEEC
YGAYTLYYKELLEKLNVSTHVFRVGTYKSAVEPYLRNDMSDAAKESASAWLTQLWDAYLD
CCHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
DVATNRQIDPKTLTMPMDQFIAKLKSVNGDLSQMMVELGLVDKLATRQEVRKDLIEQFGS
HHHCCCCCCCHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NGYDSFKQISYYDYLSQVQPTVIPDAQDIAVVVASGAIMDGTERQGTVGGDSTAALLRQA
CCCHHHHHHHHHHHHHHCCCCCCCCCHHEEEEEECCCEECCCCCCCCCCCCHHHHHHHHH
RGDDKVKAVVLRVDSPGGSAFASEVIRNEIDALKEAGKPVVVSMSSVAASGGYWISASAD
CCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHCCCCEEEECCCC
KIIAQPTTITGSIGIFGILTTFEKSLEKMGIHSDGISTSPFNGVSITRPLNDDVAQVMQL
CEEECCCEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHH
GIEHGYHRFIKLVSDHRNLSLEAVDKVAQGRVWTGKDALKHGLVDQLGDFDDAVQAAAQL
HHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHH
ANMESYNLYWVKEPLTPMEQFLEELSMNLNASVRTELFSFAPEALQPTINKVATDINMLN
HCCCCCEEEEEECCCCHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
NFNDPKGQYLFCLNCSNL
CCCCCCCCEEEEEECCCC
>Mature Secondary Structure
MKGLFHFIGKCFQAIWKLLSFTRQLVLNLFFLIFIGVIAFAFLDSTPPSTEDKIVEPKAL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEE
ILDLSGPIVEESKFREPLERITSDVLGSQKSQENVLFDIVDTIRFAAHDNNVSGLVLHLK
EEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEH
EMNETSLTKLRYIAKAINTFKAAGKPVYAIGDYYNQSQYYLASYADKVFMAPDGTVLLRG
HCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHCCEEEECCCCEEEEEC
YGAYTLYYKELLEKLNVSTHVFRVGTYKSAVEPYLRNDMSDAAKESASAWLTQLWDAYLD
CCHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
DVATNRQIDPKTLTMPMDQFIAKLKSVNGDLSQMMVELGLVDKLATRQEVRKDLIEQFGS
HHHCCCCCCCHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NGYDSFKQISYYDYLSQVQPTVIPDAQDIAVVVASGAIMDGTERQGTVGGDSTAALLRQA
CCCHHHHHHHHHHHHHHCCCCCCCCCHHEEEEEECCCEECCCCCCCCCCCCHHHHHHHHH
RGDDKVKAVVLRVDSPGGSAFASEVIRNEIDALKEAGKPVVVSMSSVAASGGYWISASAD
CCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHCCCCEEEECCCC
KIIAQPTTITGSIGIFGILTTFEKSLEKMGIHSDGISTSPFNGVSITRPLNDDVAQVMQL
CEEECCCEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHH
GIEHGYHRFIKLVSDHRNLSLEAVDKVAQGRVWTGKDALKHGLVDQLGDFDDAVQAAAQL
HHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHH
ANMESYNLYWVKEPLTPMEQFLEELSMNLNASVRTELFSFAPEALQPTINKVATDINMLN
HCCCCCEEEEEECCCCHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
NFNDPKGQYLFCLNCSNL
CCCCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3522590; 9097039; 9278503; 7973728 [H]