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The map label for this gene is helD [H]

Identifier: 59712229

GI number: 59712229

Start: 1819660

End: 1821723

Strand: Reverse

Name: helD [H]

Synonym: VF_1622

Alternate gene names: 59712229

Gene position: 1821723-1819660 (Counterclockwise)

Preceding gene: 59712232

Following gene: 59712226

Centisome position: 62.87

GC content: 37.06

Gene sequence:

>2064_bases
ATGCAGATCAAAGCAACCCCTTTCGCTCAGTGGTTAGTTCAAGGTGACTTTTATCAACTTGAATTAGAGCAAGAGCAGCT
GTTGTTTACCTCACATAAATATCAAGTATCTGTTCCATTTGATAAATGGAGTGGAAAAGTGACTTTTGAAAGAGGCCTTA
TTTGGGGAGCGCTTTGTTTTTATAATCGCAATGAACAAGTGGCATGGCGAGTATCTGGACTTCCATGGGAAGAGTGTGAT
GCCATAATTAATGCAGTGTTAAATGCTTATGCTGATTGGAAAGAATCAAAAGTATTGCTGTTAAATCAGCTGCAACCACA
GATGATTGAATTAATCGAAAATTTTAGTGCAAACAAGCGCTTTTTAAAGCAAACAGAAGCTTTAATGATGGTCGAAAAGT
TATATGATCTTTTCGAGCAGACGAAGATTTCCATTCCTTTAGCTGAACAGCTTCAACCTGATTGTATTGCCCCTGTTATT
GATTGGCTTACTGAGCCAGAAGAACAATTAAAAGTCTTGAATGATAATTGGTTAGAAGAGCAAAAAGTAGAATGGAAAAC
GTTTTTTTCTGAGTGTGAATCACAGCCACTAAATGAATCTCAGCAAAGTGCTTTGCTATTAAATGAAGACCACACCTTAG
TTTTAGCTGGAGCAGGCTCAGGTAAAACAAGTGTATTGGTTGCCCGTGTAAATTATTTAATTCAAAGTGGACAAGCTCAA
GCAGACGAAATTTTGTTATTGGCTTTTGGTCGTCAAGCTGCACAAGAAATGTCAGGTCGTATCATTGATAAACTCGGTTT
TGAAATAGGTCAACGAGTCAAAGTCGCTACTTTTCATCAATTAGGCTTAGATATTATTCGTCATGTTGAATATCAACAAC
CAAAGCTATCATGTTTAGTTACTGATGTTAAAGCCCGTAAAGAATGGTTTGTTCATGTTTTGGATTCCGAATGGAAAAAT
GCAACAGCGATGAACCGTTGGAAGAAGCACCTTAAGAAATGGCCAATTGCTTATTTGCGTGGTGATGTCGATTTAAGAGA
AGAATCGCACAATGAAAAGTTACAAGAATGGCTTTATAAACAAATTTGCCAATTAAATAGTCTGCAATTAGATAAGAAAA
GCATTGTTGAAAAGATTGAGTTTCATCCTGATGTGCAGCGTTTAAAAAGTGAATTGAATATTGTTTGGCCATTCTTTAAG
GCGTATGAAAGAACGCTGAAACATGAAGATGCTATTGATTATGAATCAATGATTACTAAGGCTAAAGCATACATTGCTGA
TAACAAGTATGAATTGCCTTGGTCATTTGTAATGGTTGATGAATATCAAGATATTTCACCACAACGTCTAGATTTGATTG
AAGCCATTTGTCACACCGAAAATAAACATCAAACTAAGGCTACGCTATTTGCTGTTGGTGATGATTGGCAAGCGATATAC
CGTTTTGCTGGTGCAGATGTGTCGCTAACTACAGGGTTTGAACATCGTTTTCAATCGACACAAATTGCTCATTTATCGAC
AACGTATCGTTTTAATGATCAAATAGGGGCGGTAGCAAATCGCTTTGTTCAAGAGAACCCTATGCAGCTTAAAAAAGAGC
TAAATAGTTTCACTAAAAAGAAAAGAAAGGCAGTGAAAGTAATAGAGCACCAATCTATAGAAAAAGAGCTCTTATCATTA
TCTAAAAACATTCGTGATGGCGAAAAAGTCGTGATTCTTGGACGTAACCATAATCATAAGCCCGATAGCTTTGACGAGTG
GGTTAAGTTCTATCCTGGTTTGAATTTGGAATATAATACGTGTCATGCTAGTAAAGGGACTGAAGCAGATTATGTCTTTA
TTGTAGAGATGACGGATGGACAATTCCCGATGAAAGAGCGGGCGGAGTCATTAGATACGGCTTTACTTCCTCATGCAGAA
AGCTTTCCATATGCTGAAGAGCGACGACTATTCTATGTCGCTTTAACTCGTGCAAAGAAAAAGGTATGGATTGTTTATCA
TGGTAAACCATCTCCATTTGTACAAGAGATCGTGGATAAAGATTATCCAGTGATAATTGAATAA

Upstream 100 bases:

>100_bases
CTGAGACAACAAAAAAAGAACCTGATATCCTATGACCTCTTCAGCAAATTCGTGAATGAAACACTCTCATAGTGTCATAT
TAATTAGGTAGTATTGAATC

Downstream 100 bases:

>100_bases
CTTAGGGACAATAAAATAGCCGCTCGAAAATAGCGGCTATTTTTTTATTAAGCTAAACGCGTTCAGCTAAATAAGCATCA
TAATCAGGAATTTCGATTGA

Product: DNA helicase IV

Products: NA

Alternate protein names: 75 kDa helicase [H]

Number of amino acids: Translated: 687; Mature: 687

Protein sequence:

>687_residues
MQIKATPFAQWLVQGDFYQLELEQEQLLFTSHKYQVSVPFDKWSGKVTFERGLIWGALCFYNRNEQVAWRVSGLPWEECD
AIINAVLNAYADWKESKVLLLNQLQPQMIELIENFSANKRFLKQTEALMMVEKLYDLFEQTKISIPLAEQLQPDCIAPVI
DWLTEPEEQLKVLNDNWLEEQKVEWKTFFSECESQPLNESQQSALLLNEDHTLVLAGAGSGKTSVLVARVNYLIQSGQAQ
ADEILLLAFGRQAAQEMSGRIIDKLGFEIGQRVKVATFHQLGLDIIRHVEYQQPKLSCLVTDVKARKEWFVHVLDSEWKN
ATAMNRWKKHLKKWPIAYLRGDVDLREESHNEKLQEWLYKQICQLNSLQLDKKSIVEKIEFHPDVQRLKSELNIVWPFFK
AYERTLKHEDAIDYESMITKAKAYIADNKYELPWSFVMVDEYQDISPQRLDLIEAICHTENKHQTKATLFAVGDDWQAIY
RFAGADVSLTTGFEHRFQSTQIAHLSTTYRFNDQIGAVANRFVQENPMQLKKELNSFTKKKRKAVKVIEHQSIEKELLSL
SKNIRDGEKVVILGRNHNHKPDSFDEWVKFYPGLNLEYNTCHASKGTEADYVFIVEMTDGQFPMKERAESLDTALLPHAE
SFPYAEERRLFYVALTRAKKKVWIVYHGKPSPFVQEIVDKDYPVIIE

Sequences:

>Translated_687_residues
MQIKATPFAQWLVQGDFYQLELEQEQLLFTSHKYQVSVPFDKWSGKVTFERGLIWGALCFYNRNEQVAWRVSGLPWEECD
AIINAVLNAYADWKESKVLLLNQLQPQMIELIENFSANKRFLKQTEALMMVEKLYDLFEQTKISIPLAEQLQPDCIAPVI
DWLTEPEEQLKVLNDNWLEEQKVEWKTFFSECESQPLNESQQSALLLNEDHTLVLAGAGSGKTSVLVARVNYLIQSGQAQ
ADEILLLAFGRQAAQEMSGRIIDKLGFEIGQRVKVATFHQLGLDIIRHVEYQQPKLSCLVTDVKARKEWFVHVLDSEWKN
ATAMNRWKKHLKKWPIAYLRGDVDLREESHNEKLQEWLYKQICQLNSLQLDKKSIVEKIEFHPDVQRLKSELNIVWPFFK
AYERTLKHEDAIDYESMITKAKAYIADNKYELPWSFVMVDEYQDISPQRLDLIEAICHTENKHQTKATLFAVGDDWQAIY
RFAGADVSLTTGFEHRFQSTQIAHLSTTYRFNDQIGAVANRFVQENPMQLKKELNSFTKKKRKAVKVIEHQSIEKELLSL
SKNIRDGEKVVILGRNHNHKPDSFDEWVKFYPGLNLEYNTCHASKGTEADYVFIVEMTDGQFPMKERAESLDTALLPHAE
SFPYAEERRLFYVALTRAKKKVWIVYHGKPSPFVQEIVDKDYPVIIE
>Mature_687_residues
MQIKATPFAQWLVQGDFYQLELEQEQLLFTSHKYQVSVPFDKWSGKVTFERGLIWGALCFYNRNEQVAWRVSGLPWEECD
AIINAVLNAYADWKESKVLLLNQLQPQMIELIENFSANKRFLKQTEALMMVEKLYDLFEQTKISIPLAEQLQPDCIAPVI
DWLTEPEEQLKVLNDNWLEEQKVEWKTFFSECESQPLNESQQSALLLNEDHTLVLAGAGSGKTSVLVARVNYLIQSGQAQ
ADEILLLAFGRQAAQEMSGRIIDKLGFEIGQRVKVATFHQLGLDIIRHVEYQQPKLSCLVTDVKARKEWFVHVLDSEWKN
ATAMNRWKKHLKKWPIAYLRGDVDLREESHNEKLQEWLYKQICQLNSLQLDKKSIVEKIEFHPDVQRLKSELNIVWPFFK
AYERTLKHEDAIDYESMITKAKAYIADNKYELPWSFVMVDEYQDISPQRLDLIEAICHTENKHQTKATLFAVGDDWQAIY
RFAGADVSLTTGFEHRFQSTQIAHLSTTYRFNDQIGAVANRFVQENPMQLKKELNSFTKKKRKAVKVIEHQSIEKELLSL
SKNIRDGEKVVILGRNHNHKPDSFDEWVKFYPGLNLEYNTCHASKGTEADYVFIVEMTDGQFPMKERAESLDTALLPHAE
SFPYAEERRLFYVALTRAKKKVWIVYHGKPSPFVQEIVDKDYPVIIE

Specific function: Helicase IV catalyzes the unwinding of duplex DNA in the 3' to 5' direction with respect to the bound single strand in a reaction that is dependent upon the hydrolysis of ATP [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase ATP-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787196, Length=697, Percent_Identity=35.2941176470588, Blast_Score=396, Evalue=1e-111,
Organism=Escherichia coli, GI48994965, Length=91, Percent_Identity=42.8571428571429, Blast_Score=72, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000212
- InterPro:   IPR014016
- InterPro:   IPR022161
- InterPro:   IPR003661 [H]

Pfam domain/function: PF12462 Nucleolin_N; PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 79999; Mature: 79999

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIKATPFAQWLVQGDFYQLELEQEQLLFTSHKYQVSVPFDKWSGKVTFERGLIWGALCF
CCCCCCHHHHHHHCCCEEEEEECHHHHEEECCCEEEEECHHCCCCEEEEECCCEEEEHEE
YNRNEQVAWRVSGLPWEECDAIINAVLNAYADWKESKVLLLNQLQPQMIELIENFSANKR
ECCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCEEEHHHCCHHHHHHHHCCCCCHH
FLKQTEALMMVEKLYDLFEQTKISIPLAEQLQPDCIAPVIDWLTEPEEQLKVLNDNWLEE
HHHHHHHHHHHHHHHHHHHHCEECCCCHHHCCCHHHHHHHHHHCCCHHHHHHHCCCCCHH
QKVEWKTFFSECESQPLNESQQSALLLNEDHTLVLAGAGSGKTSVLVARVNYLIQSGQAQ
HHHHHHHHHHHHCCCCCCCCCCCEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHCCCCC
ADEILLLAFGRQAAQEMSGRIIDKLGFEIGQRVKVATFHQLGLDIIRHVEYQQPKLSCLV
CCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHCCCCCCCCEEEE
TDVKARKEWFVHVLDSEWKNATAMNRWKKHLKKWPIAYLRGDVDLREESHNEKLQEWLYK
EHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHEEECCCCHHHHHHHHHHHHHHHH
QICQLNSLQLDKKSIVEKIEFHPDVQRLKSELNIVWPFFKAYERTLKHEDAIDYESMITK
HHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHCCEEHHHHHHHHHHHCCCCCCCHHHHHHH
AKAYIADNKYELPWSFVMVDEYQDISPQRLDLIEAICHTENKHQTKATLFAVGDDWQAIY
HHHHCCCCCEECCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHH
RFAGADVSLTTGFEHRFQSTQIAHLSTTYRFNDQIGAVANRFVQENPMQLKKELNSFTKK
HHCCCCEEEECCHHHHHCCCEEEEEEEEEEECCHHHHHHHHHHHCCHHHHHHHHHHHHHH
KRKAVKVIEHQSIEKELLSLSKNIRDGEKVVILGRNHNHKPDSFDEWVKFYPGLNLEYNT
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHCCCCCCEECE
CHASKGTEADYVFIVEMTDGQFPMKERAESLDTALLPHAESFPYAEERRLFYVALTRAKK
ECCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCHHHCEEEEEEEECCCC
KVWIVYHGKPSPFVQEIVDKDYPVIIE
EEEEEECCCCCHHHHHHHCCCCCEEEC
>Mature Secondary Structure
MQIKATPFAQWLVQGDFYQLELEQEQLLFTSHKYQVSVPFDKWSGKVTFERGLIWGALCF
CCCCCCHHHHHHHCCCEEEEEECHHHHEEECCCEEEEECHHCCCCEEEEECCCEEEEHEE
YNRNEQVAWRVSGLPWEECDAIINAVLNAYADWKESKVLLLNQLQPQMIELIENFSANKR
ECCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCEEEHHHCCHHHHHHHHCCCCCHH
FLKQTEALMMVEKLYDLFEQTKISIPLAEQLQPDCIAPVIDWLTEPEEQLKVLNDNWLEE
HHHHHHHHHHHHHHHHHHHHCEECCCCHHHCCCHHHHHHHHHHCCCHHHHHHHCCCCCHH
QKVEWKTFFSECESQPLNESQQSALLLNEDHTLVLAGAGSGKTSVLVARVNYLIQSGQAQ
HHHHHHHHHHHHCCCCCCCCCCCEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHCCCCC
ADEILLLAFGRQAAQEMSGRIIDKLGFEIGQRVKVATFHQLGLDIIRHVEYQQPKLSCLV
CCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHCCCCCCCCEEEE
TDVKARKEWFVHVLDSEWKNATAMNRWKKHLKKWPIAYLRGDVDLREESHNEKLQEWLYK
EHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHEEECCCCHHHHHHHHHHHHHHHH
QICQLNSLQLDKKSIVEKIEFHPDVQRLKSELNIVWPFFKAYERTLKHEDAIDYESMITK
HHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHCCEEHHHHHHHHHHHCCCCCCCHHHHHHH
AKAYIADNKYELPWSFVMVDEYQDISPQRLDLIEAICHTENKHQTKATLFAVGDDWQAIY
HHHHCCCCCEECCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHH
RFAGADVSLTTGFEHRFQSTQIAHLSTTYRFNDQIGAVANRFVQENPMQLKKELNSFTKK
HHCCCCEEEECCHHHHHCCCEEEEEEEEEEECCHHHHHHHHHHHCCHHHHHHHHHHHHHH
KRKAVKVIEHQSIEKELLSLSKNIRDGEKVVILGRNHNHKPDSFDEWVKFYPGLNLEYNT
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHCCCCCCEECE
CHASKGTEADYVFIVEMTDGQFPMKERAESLDTALLPHAESFPYAEERRLFYVALTRAKK
ECCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCHHHCEEEEEEEECCCC
KVWIVYHGKPSPFVQEIVDKDYPVIIE
EEEEEECCCCCHHHHHHHCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2542273; 8905232; 9278503 [H]