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The map label for this gene is pflB [H]

Identifier: 59712197

GI number: 59712197

Start: 1781699

End: 1783975

Strand: Direct

Name: pflB [H]

Synonym: VF_1590

Alternate gene names: 59712197

Gene position: 1781699-1783975 (Clockwise)

Preceding gene: 59712196

Following gene: 59712198

Centisome position: 61.49

GC content: 43.21

Gene sequence:

>2277_bases
ATGGCAGAGCAATTTGCTAAAGCTTGGACAGGTTTTGCAGACGGTGAGTGGCAAAACGAAGTTAACGTTCGCGATTTCAT
CCAAAAGAACTATGCACCATATGAAGGTGATGAAGAATTCCTAGTTTCTGAAGGTACTGAAGCAACTAACAAGCTTTGGG
CTAAAGTAATGGAAGGCATCAAACAGGAAAACGCAACTCACGCACCTGTTGATTTTGATACTGACGTTATCTCTACCATC
ACAGCTCACGATGCTGGTTACATCGAAAAAGATCTTGAAACTATCGTAGGTCTACAAACTGATGCTCCTCTAAAACGTGC
TATCATCCCTAACGGTGGTATCCGTATGGTTGAAGGTTCATGTAAAGCATACGATCGTGAATTAGATCCTTCAATTTCTA
AAATCTACTCTGAATACCGTAAAACACACAATGCTGGTGTTTTCGATATCTACACTCCAGACATCCTAAAATGTCGTAAA
TCAGGTGTTCTAACTGGTCTTCCAGATGCTTACGGCCGTGGTCGTATCATCGGTGATTACCGTCGTGTAGCACTTTACGG
TATTGATTTCCTAATCAAAGACAAACTAGCTCAATTCACCTCTCTACAAGAGCGTTTTGAGAACGGTGAAGATCTAACTG
CAACTATGCAACTTCGTGAAGAAATTGCTGAGCAACACCGTGCACTAGGTCAAATCAAGCAAATGGCTGCGAAATACGGT
TACGATATTTCTGGTCCTGCTACTACTGCTCAAGAAGCTGTTCAATGGACTTACTTCGGCTACCTAGCTGCTGTTAAATC
TCAAAACGGTGCTGCAATGTCTCTAGGTCGTACTTCGACTTTCCTAGACATCTACATCCAACGTGATCTTGATGCTGGTC
TTATTACTGAAGAACAAGCTCAAGAAATGATCGACCACTTCGTAATGAAGCTTCGTATGGTTCGTTTCCTACGTACTCCT
GAATACGATGAGCTATTCTCTGGTGACCCAATCTGGGCAACAGAATCTATGGGTGGTATGGGTCTTGACGGTCGTACACT
AGTTACACGTACTAACTTCCGTTTCCTAAACAGCCTATACACTATGGGTCCTTCTCCAGAGCCAAACATCACTGTTCTTT
GGTCTGAGCAACTACCTGACGGCTTCAAACGTTTCTGTGCGAAAGTATCTATCGATACTTCTTCTATCCAGTACGAAAAT
GATGACCTAATGCGTCCTGACCTACAATCTGATGACTACGCAATCGCTTGTTGTGTATCTCCAATGGTTGTTGGTAAGCA
AATGCAATTCTTTGGTGCTCGTGCTAACCTTGCAAAAACTATGCTTTACGCAATCAACGGTGGTGTTGATGAGAAGCTGA
AAATGCAAGTTGGTCCTAAAGAAGCGCCAATGGCTGACGAAGTTCTTGATTACGCTAAAGTAATGGATCGTCTAGATCAC
TTCATGGATTGGTTAGCTAAACAATACGTGACTGCTCTAAACAGCATCCACTACATGCACGATAAATACAGCTACGAAGC
GTCTCTAATGGCTCTTCACGATCGTGACGTTCGTCGTACAATGGCTTGTGGTATCGCTGGTCTTTCTGTTGCAGCTGACT
CACTATCTGCAATCAAATACGCTACAGTTAAACCTGTACGTGACGAAGACGGCATCGCTATCGATTTCGAAATCGAAGGT
GATTACCCTAAATTTGGTAACAACGACCCACGCGTTGATGATATCGCATGTGAACTAGTTTCTACGTTTATGGGCAAAAT
CCGTAAACTGAAAATGTACCGTAACGCTATCCCAACTCAATCTATCCTTACTATTACATCTAACGTTGTATACGGTAAGA
AAACTGGTAATACACCAGATGGTCGTCAAGCAGGTGCTCCTTTTGCTCCTGGTGCTAACCCAATGCACGGCCGTGATGAG
AAAGGTGCTGTAGCATCTCTAACTTCAGTAGGTAAACTACCGTTTGCTGATGCACAAGATGGTATCTCTTACACATTCTC
TATCGTACCTAACGCTCTAGGTAAAGACGATGACAACCGTCGTTCTAACCTTGCTGGCCTAATGGATGGTTACTTCCACC
ACGAAGCAAACATCGAAGGCGGTCAACACTTGAACGTTAACGTTCTTAACCGTGAAACTCTAGAAGACGCAGTTAAGCAC
CCAGAGAACTACCCACAACTAACTATCCGTGTATCGGGTTACGCTGTGCGTTTTAACTCTCTAACTACTGAACAACAAAA
AGACGTAATCGCACGTACTTTCACTGAATCACTATAA

Upstream 100 bases:

>100_bases
ATTAAATTGAGTGTACTATTTTGCCTTCTAACGAAGGATATGGGTATCTCAAACCCATTTTAATAGTACGTATTTTTTCT
ACTAAAAAGGTAGGTATATC

Downstream 100 bases:

>100_bases
TTCAGCGTTAATTAAGTAGAATTGAGCCCTGCCATTTTGGTGGGGCTTTTTTATTGCCTTATTAAATTAAAGTTTATCTC
CCCTTTCTCTTCTTCATTAC

Product: pyruvate formate lyase I

Products: NA

Alternate protein names: Pyruvate formate-lyase 1 [H]

Number of amino acids: Translated: 758; Mature: 757

Protein sequence:

>758_residues
MAEQFAKAWTGFADGEWQNEVNVRDFIQKNYAPYEGDEEFLVSEGTEATNKLWAKVMEGIKQENATHAPVDFDTDVISTI
TAHDAGYIEKDLETIVGLQTDAPLKRAIIPNGGIRMVEGSCKAYDRELDPSISKIYSEYRKTHNAGVFDIYTPDILKCRK
SGVLTGLPDAYGRGRIIGDYRRVALYGIDFLIKDKLAQFTSLQERFENGEDLTATMQLREEIAEQHRALGQIKQMAAKYG
YDISGPATTAQEAVQWTYFGYLAAVKSQNGAAMSLGRTSTFLDIYIQRDLDAGLITEEQAQEMIDHFVMKLRMVRFLRTP
EYDELFSGDPIWATESMGGMGLDGRTLVTRTNFRFLNSLYTMGPSPEPNITVLWSEQLPDGFKRFCAKVSIDTSSIQYEN
DDLMRPDLQSDDYAIACCVSPMVVGKQMQFFGARANLAKTMLYAINGGVDEKLKMQVGPKEAPMADEVLDYAKVMDRLDH
FMDWLAKQYVTALNSIHYMHDKYSYEASLMALHDRDVRRTMACGIAGLSVAADSLSAIKYATVKPVRDEDGIAIDFEIEG
DYPKFGNNDPRVDDIACELVSTFMGKIRKLKMYRNAIPTQSILTITSNVVYGKKTGNTPDGRQAGAPFAPGANPMHGRDE
KGAVASLTSVGKLPFADAQDGISYTFSIVPNALGKDDDNRRSNLAGLMDGYFHHEANIEGGQHLNVNVLNRETLEDAVKH
PENYPQLTIRVSGYAVRFNSLTTEQQKDVIARTFTESL

Sequences:

>Translated_758_residues
MAEQFAKAWTGFADGEWQNEVNVRDFIQKNYAPYEGDEEFLVSEGTEATNKLWAKVMEGIKQENATHAPVDFDTDVISTI
TAHDAGYIEKDLETIVGLQTDAPLKRAIIPNGGIRMVEGSCKAYDRELDPSISKIYSEYRKTHNAGVFDIYTPDILKCRK
SGVLTGLPDAYGRGRIIGDYRRVALYGIDFLIKDKLAQFTSLQERFENGEDLTATMQLREEIAEQHRALGQIKQMAAKYG
YDISGPATTAQEAVQWTYFGYLAAVKSQNGAAMSLGRTSTFLDIYIQRDLDAGLITEEQAQEMIDHFVMKLRMVRFLRTP
EYDELFSGDPIWATESMGGMGLDGRTLVTRTNFRFLNSLYTMGPSPEPNITVLWSEQLPDGFKRFCAKVSIDTSSIQYEN
DDLMRPDLQSDDYAIACCVSPMVVGKQMQFFGARANLAKTMLYAINGGVDEKLKMQVGPKEAPMADEVLDYAKVMDRLDH
FMDWLAKQYVTALNSIHYMHDKYSYEASLMALHDRDVRRTMACGIAGLSVAADSLSAIKYATVKPVRDEDGIAIDFEIEG
DYPKFGNNDPRVDDIACELVSTFMGKIRKLKMYRNAIPTQSILTITSNVVYGKKTGNTPDGRQAGAPFAPGANPMHGRDE
KGAVASLTSVGKLPFADAQDGISYTFSIVPNALGKDDDNRRSNLAGLMDGYFHHEANIEGGQHLNVNVLNRETLEDAVKH
PENYPQLTIRVSGYAVRFNSLTTEQQKDVIARTFTESL
>Mature_757_residues
AEQFAKAWTGFADGEWQNEVNVRDFIQKNYAPYEGDEEFLVSEGTEATNKLWAKVMEGIKQENATHAPVDFDTDVISTIT
AHDAGYIEKDLETIVGLQTDAPLKRAIIPNGGIRMVEGSCKAYDRELDPSISKIYSEYRKTHNAGVFDIYTPDILKCRKS
GVLTGLPDAYGRGRIIGDYRRVALYGIDFLIKDKLAQFTSLQERFENGEDLTATMQLREEIAEQHRALGQIKQMAAKYGY
DISGPATTAQEAVQWTYFGYLAAVKSQNGAAMSLGRTSTFLDIYIQRDLDAGLITEEQAQEMIDHFVMKLRMVRFLRTPE
YDELFSGDPIWATESMGGMGLDGRTLVTRTNFRFLNSLYTMGPSPEPNITVLWSEQLPDGFKRFCAKVSIDTSSIQYEND
DLMRPDLQSDDYAIACCVSPMVVGKQMQFFGARANLAKTMLYAINGGVDEKLKMQVGPKEAPMADEVLDYAKVMDRLDHF
MDWLAKQYVTALNSIHYMHDKYSYEASLMALHDRDVRRTMACGIAGLSVAADSLSAIKYATVKPVRDEDGIAIDFEIEGD
YPKFGNNDPRVDDIACELVSTFMGKIRKLKMYRNAIPTQSILTITSNVVYGKKTGNTPDGRQAGAPFAPGANPMHGRDEK
GAVASLTSVGKLPFADAQDGISYTFSIVPNALGKDDDNRRSNLAGLMDGYFHHEANIEGGQHLNVNVLNRETLEDAVKHP
ENYPQLTIRVSGYAVRFNSLTTEQQKDVIARTFTESL

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1787131, Length=758, Percent_Identity=82.3218997361478, Blast_Score=1338, Evalue=0.0,
Organism=Escherichia coli, GI48994926, Length=754, Percent_Identity=74.8010610079576, Blast_Score=1224, Evalue=0.0,
Organism=Escherichia coli, GI1787044, Length=629, Percent_Identity=27.6629570747218, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI1790388, Length=638, Percent_Identity=25.0783699059561, Blast_Score=134, Evalue=3e-32,
Organism=Escherichia coli, GI1788933, Length=64, Percent_Identity=89.0625, Blast_Score=121, Evalue=1e-28,

Paralogues:

None

Copy number: 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005949
- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 84556; Mature: 84425

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEQFAKAWTGFADGEWQNEVNVRDFIQKNYAPYEGDEEFLVSEGTEATNKLWAKVMEGI
CCHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHHHH
KQENATHAPVDFDTDVISTITAHDAGYIEKDLETIVGLQTDAPLKRAIIPNGGIRMVEGS
HHCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHCCCCCCEEEEECC
CKAYDRELDPSISKIYSEYRKTHNAGVFDIYTPDILKCRKSGVLTGLPDAYGRGRIIGDY
HHHHCCCCCHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHCCCCCCCCCCCCCCCEEHHH
RRVALYGIDFLIKDKLAQFTSLQERFENGEDLTATMQLREEIAEQHRALGQIKQMAAKYG
HEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
YDISGPATTAQEAVQWTYFGYLAAVKSQNGAAMSLGRTSTFLDIYIQRDLDAGLITEEQA
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCEEEEEEEECCCCCCCCCHHHH
QEMIDHFVMKLRMVRFLRTPEYDELFSGDPIWATESMGGMGLDGRTLVTRTNFRFLNSLY
HHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCEEECCCCCCCCCCCEEEEECHHHHHHHHH
TMGPSPEPNITVLWSEQLPDGFKRFCAKVSIDTSSIQYENDDLMRPDLQSDDYAIACCVS
HCCCCCCCCEEEEECCCCCHHHHHHHHHEECCCCCEEECCCCCCCCCCCCCCCEEEEECC
PMVVGKQMQFFGARANLAKTMLYAINGGVDEKLKMQVGPKEAPMADEVLDYAKVMDRLDH
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHH
FMDWLAKQYVTALNSIHYMHDKYSYEASLMALHDRDVRRTMACGIAGLSVAADSLSAIKY
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHEE
ATVKPVRDEDGIAIDFEIEGDYPKFGNNDPRVDDIACELVSTFMGKIRKLKMYRNAIPTQ
EEECCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
SILTITSNVVYGKKTGNTPDGRQAGAPFAPGANPMHGRDEKGAVASLTSVGKLPFADAQD
HHHEECCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCC
GISYTFSIVPNALGKDDDNRRSNLAGLMDGYFHHEANIEGGQHLNVNVLNRETLEDAVKH
CCEEEEEECCHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHC
PENYPQLTIRVSGYAVRFNSLTTEQQKDVIARTFTESL
CCCCCEEEEEEEEEEEEECCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AEQFAKAWTGFADGEWQNEVNVRDFIQKNYAPYEGDEEFLVSEGTEATNKLWAKVMEGI
CHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHHHH
KQENATHAPVDFDTDVISTITAHDAGYIEKDLETIVGLQTDAPLKRAIIPNGGIRMVEGS
HHCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHCCCCCCEEEEECC
CKAYDRELDPSISKIYSEYRKTHNAGVFDIYTPDILKCRKSGVLTGLPDAYGRGRIIGDY
HHHHCCCCCHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHCCCCCCCCCCCCCCCEEHHH
RRVALYGIDFLIKDKLAQFTSLQERFENGEDLTATMQLREEIAEQHRALGQIKQMAAKYG
HEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
YDISGPATTAQEAVQWTYFGYLAAVKSQNGAAMSLGRTSTFLDIYIQRDLDAGLITEEQA
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCEEEEEEEECCCCCCCCCHHHH
QEMIDHFVMKLRMVRFLRTPEYDELFSGDPIWATESMGGMGLDGRTLVTRTNFRFLNSLY
HHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCEEECCCCCCCCCCCEEEEECHHHHHHHHH
TMGPSPEPNITVLWSEQLPDGFKRFCAKVSIDTSSIQYENDDLMRPDLQSDDYAIACCVS
HCCCCCCCCEEEEECCCCCHHHHHHHHHEECCCCCEEECCCCCCCCCCCCCCCEEEEECC
PMVVGKQMQFFGARANLAKTMLYAINGGVDEKLKMQVGPKEAPMADEVLDYAKVMDRLDH
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHH
FMDWLAKQYVTALNSIHYMHDKYSYEASLMALHDRDVRRTMACGIAGLSVAADSLSAIKY
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHEE
ATVKPVRDEDGIAIDFEIEGDYPKFGNNDPRVDDIACELVSTFMGKIRKLKMYRNAIPTQ
EEECCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
SILTITSNVVYGKKTGNTPDGRQAGAPFAPGANPMHGRDEKGAVASLTSVGKLPFADAQD
HHHEECCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCC
GISYTFSIVPNALGKDDDNRRSNLAGLMDGYFHHEANIEGGQHLNVNVLNRETLEDAVKH
CCEEEEEECCHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHC
PENYPQLTIRVSGYAVRFNSLTTEQQKDVIARTFTESL
CCCCCEEEEEEEEEEEEECCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3053170; 8905232; 9278503; 2651404; 1310545; 9629924; 10504733 [H]