Click here to switch to the map view.

The map label for this gene is pflA [H]

Identifier: 59712198

GI number: 59712198

Start: 1784138

End: 1784875

Strand: Direct

Name: pflA [H]

Synonym: VF_1591

Alternate gene names: 59712198

Gene position: 1784138-1784875 (Clockwise)

Preceding gene: 59712197

Following gene: 59712199

Centisome position: 61.57

GC content: 37.53

Gene sequence:

>738_bases
ATGTCTGTAGGTCGTATTCACTCTTTTGAAACATGTGGAACTGTTGATGGTCCTGGTATTCGCTTTATTGTTTTCTTGCA
AGGCTGTTTAATGCGTTGTATGTATTGCCACAACCGTGATACTTGGGATACACATGATGGTAAAGAAGTGACTGTTGCTG
AATTGATTGAAGAAGCAAAAAGCTATCGTCACTTTATGAAAGCCTCAGGAGGCGGTATTACTTGTTCAGGCGGTGAAGCA
ATGTTACAACCTGAATTTGTACGTGATTTTTTCCGTGCAGCTCAAGCAGAAGGCATCCATACTTGTTTAGATACCAATGG
GTATATTCGTAAACACACAGATGTGATTGATGAAGTTTTAGAAGCATCAGATCTTGTTATGCTTGATCTAAAACAGATGA
AAGATGACGTCCATAAAGAATTCATTGGTGTATCAAATACTCGAGTTCTTGATTTTGCTCGTTACCTTCATAAGATTGGT
CAAAAAACATGGATCCGTTACGTTATTGTTCCTGGCTATACTGATGATGAAGAATCAGCACACCTTTTAGGTAACTTTAT
TAAAGATATGGATAACATTGAAAAAATAGAATTACTTCCATACCACAAATTAGGTGCCCATAAATGGGAAGCATTAGGCC
ATGACTACCCTCTTGAAGGCGTTAATCCACCATCAAAAGAAACGATGGAAAAAATGGTCGAAATCCTAAGCCAATACCAC
AACAACGTAAAATATTAA

Upstream 100 bases:

>100_bases
TAAATTAAAGTTTATCTCCCCTTTCTCTTCTTCATTACTACTTAATTGACCGCTTTTATAATAAAATAACCCCATAATTA
TAGTTCGGAGAAATGTCGTC

Downstream 100 bases:

>100_bases
TTACGTCGTTCCCTTTTTAAGCAACACAAATGCCCGATCTTCGGGCATTTTTATTGGAAAAAATAATGTACCAATTAAGC
TTTTCGCTACAAGAATTCCT

Product: pyruvate formate lyase-activating enzyme 1

Products: NA

Alternate protein names: Formate-C-acetyltransferase-activating enzyme 1; PFL-activating enzyme 1 [H]

Number of amino acids: Translated: 245; Mature: 244

Protein sequence:

>245_residues
MSVGRIHSFETCGTVDGPGIRFIVFLQGCLMRCMYCHNRDTWDTHDGKEVTVAELIEEAKSYRHFMKASGGGITCSGGEA
MLQPEFVRDFFRAAQAEGIHTCLDTNGYIRKHTDVIDEVLEASDLVMLDLKQMKDDVHKEFIGVSNTRVLDFARYLHKIG
QKTWIRYVIVPGYTDDEESAHLLGNFIKDMDNIEKIELLPYHKLGAHKWEALGHDYPLEGVNPPSKETMEKMVEILSQYH
NNVKY

Sequences:

>Translated_245_residues
MSVGRIHSFETCGTVDGPGIRFIVFLQGCLMRCMYCHNRDTWDTHDGKEVTVAELIEEAKSYRHFMKASGGGITCSGGEA
MLQPEFVRDFFRAAQAEGIHTCLDTNGYIRKHTDVIDEVLEASDLVMLDLKQMKDDVHKEFIGVSNTRVLDFARYLHKIG
QKTWIRYVIVPGYTDDEESAHLLGNFIKDMDNIEKIELLPYHKLGAHKWEALGHDYPLEGVNPPSKETMEKMVEILSQYH
NNVKY
>Mature_244_residues
SVGRIHSFETCGTVDGPGIRFIVFLQGCLMRCMYCHNRDTWDTHDGKEVTVAELIEEAKSYRHFMKASGGGITCSGGEAM
LQPEFVRDFFRAAQAEGIHTCLDTNGYIRKHTDVIDEVLEASDLVMLDLKQMKDDVHKEFIGVSNTRVLDFARYLHKIGQ
KTWIRYVIVPGYTDDEESAHLLGNFIKDMDNIEKIELLPYHKLGAHKWEALGHDYPLEGVNPPSKETMEKMVEILSQYHN
NVKY

Specific function: Activation of pyruvate formate-lyase 1 under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family [H]

Homologues:

Organism=Escherichia coli, GI1787130, Length=243, Percent_Identity=67.4897119341564, Blast_Score=367, Evalue=1e-103,
Organism=Escherichia coli, GI1790389, Length=265, Percent_Identity=27.1698113207547, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI226510931, Length=148, Percent_Identity=31.0810810810811, Blast_Score=74, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR012838
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 28003; Mature: 27872

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HCCCC
>Mature Secondary Structure 
SVGRIHSFETCGTVDGPGIRFIVFLQGCLMRCMYCHNRDTWDTHDGKEVTVAELIEEAK
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHH
SYRHFMKASGGGITCSGGEAMLQPEFVRDFFRAAQAEGIHTCLDTNGYIRKHTDVIDEVL
HHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHH
EASDLVMLDLKQMKDDVHKEFIGVSNTRVLDFARYLHKIGQKTWIRYVIVPGYTDDEESA
HHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCHHHEEEEEEECCCCCCCHHH
HLLGNFIKDMDNIEKIELLPYHKLGAHKWEALGHDYPLEGVNPPSKETMEKMVEILSQYH
HHHHHHHHHHHCHHHEEECCCHHHCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
NNVKY
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]