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The map label for this gene is nrfB [H]

Identifier: 59712160

GI number: 59712160

Start: 1736696

End: 1737289

Strand: Reverse

Name: nrfB [H]

Synonym: VF_1553

Alternate gene names: 59712160

Gene position: 1737289-1736696 (Counterclockwise)

Preceding gene: 59712162

Following gene: 59712159

Centisome position: 59.96

GC content: 41.58

Gene sequence:

>594_bases
ATGGGCAAAGTCAAATTAGCCATAGCCACAATGTTGAAGCTTACCTTCGCATTTTGTCTCTATGGTTTGTCTCTGAATGT
TGCTGCAGATGAATCGCCCCAAGTAGAGGCCAACTCATCCAATCGACATGAAGTGACATTAATTCGTGATCGCGATTATG
CGTGTACACAATGTCATAAAGACGAAAAAGAGACACTTAAAGGATCACACGGGGAAGAGTCATTTGCGATTCTTAAACGT
GATGTGAATTGTGTCGAGTGTCATAAATCAATTGGGCCTGATCACCGAGAAGGCGCACCGCAAGTTATAAAATATTCGGC
AGCACAATCAAAAGTCGGCACGGAAAAAGTGACCCTCTCGTTTGATGAAATTTTACAAGCTAATAGCCAATGTACTGATT
GTCATACGCCTGATCGATTACGTGAAGATAGCTGGACACACGACGTTCACGCTAAAAACCTAACCTGCTCATCTTGTCAT
ATCGTTCATGGAGAGAAAGAAGCTGTGCTTTCTTTTGACCATTCAGCGAAGATTAAGATGTGTGTTGATTGCCACGTTGA
TTTTAATCAGAAAGAAAATAAGCTAGGAGAATAG

Upstream 100 bases:

>100_bases
TGAGAATTGATACCAAAGAAGAGCACTAGCTCTTTAACAACAATTACTCATCCAACTATTTATGAATTTGATAAATAGAA
TGGAAAGGGAAGGATATAAA

Downstream 100 bases:

>100_bases
TATGAGTTGTTCTAGACGAAATTTTTTAGCTGGTTCAGGTGCGGTTATTTTCACAACAGGTGTTGCGACAACATCATTAA
TAAACAGTAAAAAAACCTTA

Product: cytochrome c nitrite reductase pentaheme subunit

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: NA

Number of amino acids: Translated: 197; Mature: 196

Protein sequence:

>197_residues
MGKVKLAIATMLKLTFAFCLYGLSLNVAADESPQVEANSSNRHEVTLIRDRDYACTQCHKDEKETLKGSHGEESFAILKR
DVNCVECHKSIGPDHREGAPQVIKYSAAQSKVGTEKVTLSFDEILQANSQCTDCHTPDRLREDSWTHDVHAKNLTCSSCH
IVHGEKEAVLSFDHSAKIKMCVDCHVDFNQKENKLGE

Sequences:

>Translated_197_residues
MGKVKLAIATMLKLTFAFCLYGLSLNVAADESPQVEANSSNRHEVTLIRDRDYACTQCHKDEKETLKGSHGEESFAILKR
DVNCVECHKSIGPDHREGAPQVIKYSAAQSKVGTEKVTLSFDEILQANSQCTDCHTPDRLREDSWTHDVHAKNLTCSSCH
IVHGEKEAVLSFDHSAKIKMCVDCHVDFNQKENKLGE
>Mature_196_residues
GKVKLAIATMLKLTFAFCLYGLSLNVAADESPQVEANSSNRHEVTLIRDRDYACTQCHKDEKETLKGSHGEESFAILKRD
VNCVECHKSIGPDHREGAPQVIKYSAAQSKVGTEKVTLSFDEILQANSQCTDCHTPDRLREDSWTHDVHAKNLTCSSCHI
VHGEKEAVLSFDHSAKIKMCVDCHVDFNQKENKLGE

Specific function: Plays a role in nitrite reduction [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082366, Length=188, Percent_Identity=32.9787234042553, Blast_Score=114, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017564
- InterPro:   IPR011031 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 21974; Mature: 21843

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: PS51008 MULTIHEME_CYTC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
5.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKVKLAIATMLKLTFAFCLYGLSLNVAADESPQVEANSSNRHEVTLIRDRDYACTQCHK
CCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCEECCCCCCEEEEEEECCCCHHHHHCC
DEKETLKGSHGEESFAILKRDVNCVECHKSIGPDHREGAPQVIKYSAAQSKVGTEKVTLS
CHHHHHCCCCCCHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHEEEHHHHHCCCCEEEEEE
FDEILQANSQCTDCHTPDRLREDSWTHDVHAKNLTCSSCHIVHGEKEAVLSFDHSAKIKM
HHHHHHCCCCCCCCCCCHHHCCCCCCCCCCCCCCEECCEEEEECCCCEEEEECCCCEEEE
CVDCHVDFNQKENKLGE
EEEEEECCCCCCCCCCC
>Mature Secondary Structure 
GKVKLAIATMLKLTFAFCLYGLSLNVAADESPQVEANSSNRHEVTLIRDRDYACTQCHK
CCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCEECCCCCCEEEEEEECCCCHHHHHCC
DEKETLKGSHGEESFAILKRDVNCVECHKSIGPDHREGAPQVIKYSAAQSKVGTEKVTLS
CHHHHHCCCCCCHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHEEEHHHHHCCCCEEEEEE
FDEILQANSQCTDCHTPDRLREDSWTHDVHAKNLTCSSCHIVHGEKEAVLSFDHSAKIKM
HHHHHHCCCCCCCCCCCHHHCCCCCCCCCCCCCCEECCEEEEECCCCEEEEECCCCEEEE
CVDCHVDFNQKENKLGE
EEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]