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The map label for this gene is nfrC [H]

Identifier: 59712159

GI number: 59712159

Start: 1736008

End: 1736694

Strand: Reverse

Name: nfrC [H]

Synonym: VF_1552

Alternate gene names: 59712159

Gene position: 1736694-1736008 (Counterclockwise)

Preceding gene: 59712160

Following gene: 59712158

Centisome position: 59.94

GC content: 40.03

Gene sequence:

>687_bases
ATGAGTTGTTCTAGACGAAATTTTTTAGCTGGTTCAGGTGCGGTTATTTTCACAACAGGTGTTGCGACAACATCATTAAT
AAACAGTAAAAAAACCTTAGCGTATTCCATGGAAGATGGTTCAAAGCGTTATGGTATGGTTCATGATGAAAACGCGTGTA
TCGGCTGCACAGCATGTACGGAAGCATGTAGAGAAGTAAACCGTGTTCCTGAAGGGGTAACACGTTTAGAAATCATCCGT
AGTGAGCCCAAAGGTGAGTTTCCTGATGTTGAATACCGTTTTACTCGTAAGTCATGTCAACATTGTGAAAATGCTCCTTG
TGTAATGGTGTGTCCTACCGGTGCGGCATATAAAGATGAAACAACAGGAATTGTTGATGTTCATAACGAAAAATGTGTTG
GCTGTGGCTATTGTTTGGCAGCTTGTCCGTATCAAGTTCGTTTCTTTAATCCTGTGACTAAATCGGCAGATAAGTGTGAT
TTTTGCCGTGAAACAAACTTAGCAGAAGGTAAGCAACCGGCTTGTGTTGAATCTTGTCCGACAAAAGCACTGACCTTTGG
TGATTTAAACGATAAAGATAGCGAGATTAATGCGGTATTGAATAGTAACCCAGTATACCGTGACAAAGTGGATCTAGGTA
CCAAGCCTAAATTGTATAAGATTCCATATTTAAAAGGGGAGATTTAA

Upstream 100 bases:

>100_bases
AAAGAAGCTGTGCTTTCTTTTGACCATTCAGCGAAGATTAAGATGTGTGTTGATTGCCACGTTGATTTTAATCAGAAAGA
AAATAAGCTAGGAGAATAGT

Downstream 100 bases:

>100_bases
GATGAGTGCATGGGAAGCAGCGTTTAATTTCGACTCTTTAGTATGGGATTGGATCATTGCGATCTACCTTTTCTTAGCCG
GTATGTCTGCTGGTGCGGTT

Product: formate-dependent nitrite reductase, 4Fe4S subunit

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: NA

Number of amino acids: Translated: 228; Mature: 227

Protein sequence:

>228_residues
MSCSRRNFLAGSGAVIFTTGVATTSLINSKKTLAYSMEDGSKRYGMVHDENACIGCTACTEACREVNRVPEGVTRLEIIR
SEPKGEFPDVEYRFTRKSCQHCENAPCVMVCPTGAAYKDETTGIVDVHNEKCVGCGYCLAACPYQVRFFNPVTKSADKCD
FCRETNLAEGKQPACVESCPTKALTFGDLNDKDSEINAVLNSNPVYRDKVDLGTKPKLYKIPYLKGEI

Sequences:

>Translated_228_residues
MSCSRRNFLAGSGAVIFTTGVATTSLINSKKTLAYSMEDGSKRYGMVHDENACIGCTACTEACREVNRVPEGVTRLEIIR
SEPKGEFPDVEYRFTRKSCQHCENAPCVMVCPTGAAYKDETTGIVDVHNEKCVGCGYCLAACPYQVRFFNPVTKSADKCD
FCRETNLAEGKQPACVESCPTKALTFGDLNDKDSEINAVLNSNPVYRDKVDLGTKPKLYKIPYLKGEI
>Mature_227_residues
SCSRRNFLAGSGAVIFTTGVATTSLINSKKTLAYSMEDGSKRYGMVHDENACIGCTACTEACREVNRVPEGVTRLEIIRS
EPKGEFPDVEYRFTRKSCQHCENAPCVMVCPTGAAYKDETTGIVDVHNEKCVGCGYCLAACPYQVRFFNPVTKSADKCDF
CRETNLAEGKQPACVESCPTKALTFGDLNDKDSEINAVLNSNPVYRDKVDLGTKPKLYKIPYLKGEI

Specific function: Probably involved in the transfer of electrons from the quinone pool to the type-c cytochromes [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI2367345, Length=228, Percent_Identity=62.719298245614, Blast_Score=308, Evalue=2e-85,
Organism=Escherichia coli, GI226510944, Length=220, Percent_Identity=47.7272727272727, Blast_Score=212, Evalue=2e-56,
Organism=Escherichia coli, GI1789370, Length=259, Percent_Identity=30.5019305019305, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1787122, Length=192, Percent_Identity=34.8958333333333, Blast_Score=100, Evalue=7e-23,
Organism=Escherichia coli, GI1787749, Length=154, Percent_Identity=33.1168831168831, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI1790326, Length=171, Percent_Identity=31.5789473684211, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787872, Length=192, Percent_Identity=34.8958333333333, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787740, Length=114, Percent_Identity=34.2105263157895, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1787478, Length=134, Percent_Identity=30.5970149253731, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1789067, Length=160, Percent_Identity=32.5, Blast_Score=74, Evalue=7e-15,
Organism=Escherichia coli, GI1788811, Length=154, Percent_Identity=33.1168831168831, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI87082179, Length=138, Percent_Identity=34.7826086956522, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787963, Length=202, Percent_Identity=27.2277227722772, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI2367245, Length=158, Percent_Identity=32.9113924050633, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI87082180, Length=137, Percent_Identity=35.036496350365, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1789079, Length=182, Percent_Identity=29.6703296703297, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR017567
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 25035; Mature: 24903

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

7.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
9.2 %Cys+Met (Translated Protein)
7.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
8.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSCSRRNFLAGSGAVIFTTGVATTSLINSKKTLAYSMEDGSKRYGMVHDENACIGCTACT
CCCCCCCEECCCCCEEEEECCHHHHHHCCCCEEEEECCCCCCCCCEEECCCCEEEHHHHH
EACREVNRVPEGVTRLEIIRSEPKGEFPDVEYRFTRKSCQHCENAPCVMVCPTGAAYKDE
HHHHHHHHCCCCHHHHHHHHCCCCCCCCCCEEEECHHHHHHHCCCCEEEECCCCCCCCCC
TTGIVDVHNEKCVGCGYCLAACPYQVRFFNPVTKSADKCDFCRETNLAEGKQPACVESCP
CCCEEEECCCCCCCCHHHHHHCCEEEEEECHHHCCCCHHHHHHCCCCCCCCCCCHHHHCC
TKALTFGDLNDKDSEINAVLNSNPVYRDKVDLGTKPKLYKIPYLKGEI
CCEEEECCCCCCCCCEEEEECCCCCEECCCCCCCCCCEEECCCCCCCC
>Mature Secondary Structure 
SCSRRNFLAGSGAVIFTTGVATTSLINSKKTLAYSMEDGSKRYGMVHDENACIGCTACT
CCCCCCEECCCCCEEEEECCHHHHHHCCCCEEEEECCCCCCCCCEEECCCCEEEHHHHH
EACREVNRVPEGVTRLEIIRSEPKGEFPDVEYRFTRKSCQHCENAPCVMVCPTGAAYKDE
HHHHHHHHCCCCHHHHHHHHCCCCCCCCCCEEEECHHHHHHHCCCCEEEECCCCCCCCCC
TTGIVDVHNEKCVGCGYCLAACPYQVRFFNPVTKSADKCDFCRETNLAEGKQPACVESCP
CCCEEEECCCCCCCCHHHHHHCCEEEEEECHHHCCCCHHHHHHCCCCCCCCCCCHHHHCC
TKALTFGDLNDKDSEINAVLNSNPVYRDKVDLGTKPKLYKIPYLKGEI
CCEEEECCCCCCCCCEEEEECCCCCEECCCCCCCCCCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]