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The map label for this gene is nrfF [H]

Identifier: 59712155

GI number: 59712155

Start: 1732116

End: 1732565

Strand: Reverse

Name: nrfF [H]

Synonym: VF_1548

Alternate gene names: 59712155

Gene position: 1732565-1732116 (Counterclockwise)

Preceding gene: 59712156

Following gene: 59712152

Centisome position: 59.79

GC content: 34.22

Gene sequence:

>450_bases
ATGAAGCATTCTTTTAAATCATTACTGTATTTATTCTGTTTATTGAGTATGTTTTCAGTCAACGCTGCATTAGATAATAG
TAATGACTTATTCGTTTCTGCAGATAAAGACGTTATTGAGAGCGTTGAATTGTTTGAGTTCACCTCCCAAGAGCAACAAA
AGCAGGCGATTGAATTGGCTAAACGCTTGCGATGTCCACAATGTCAAAATCAAAACTTAATAGAGTCCAACTCACCTATT
GCTAAAGATTTACGATTAACTGTGTATCAACAGATTAAAGCAGGAAAGAGTGAAGAAGAAGTGATTGCTTTTATGACGCA
TCGATTTGGTGATTTTGTATTATATGAACCGCCATTAAATGCTAAAACATACGTACTTTGGTTATTACCATTATTGTTAC
TTGTCGTTTTTTGCATCATGGCGGTGAATTCAGTAAGAAGAAATCGTTAA

Upstream 100 bases:

>100_bases
TTGTTATTGATCAACATGGCAAGATATTACATCGATTTACAGGCGCGTTGAATGAAGAAAAGTGGCAACGTGAATTTGCC
GATTTTTTTGAGGGAAAATA

Downstream 100 bases:

>100_bases
CTTGCTAAAGCGGCTTGTTCTTCATAAGACCAATCAACACAACACTTAGCTAAGATTTCCAGTGAGTCTAAACATTGTTG
TGGTTGCTTGAGGTTTTGTT

Product: heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 149; Mature: 149

Protein sequence:

>149_residues
MKHSFKSLLYLFCLLSMFSVNAALDNSNDLFVSADKDVIESVELFEFTSQEQQKQAIELAKRLRCPQCQNQNLIESNSPI
AKDLRLTVYQQIKAGKSEEEVIAFMTHRFGDFVLYEPPLNAKTYVLWLLPLLLLVVFCIMAVNSVRRNR

Sequences:

>Translated_149_residues
MKHSFKSLLYLFCLLSMFSVNAALDNSNDLFVSADKDVIESVELFEFTSQEQQKQAIELAKRLRCPQCQNQNLIESNSPI
AKDLRLTVYQQIKAGKSEEEVIAFMTHRFGDFVLYEPPLNAKTYVLWLLPLLLLVVFCIMAVNSVRRNR
>Mature_149_residues
MKHSFKSLLYLFCLLSMFSVNAALDNSNDLFVSADKDVIESVELFEFTSQEQQKQAIELAKRLRCPQCQNQNLIESNSPI
AKDLRLTVYQQIKAGKSEEEVIAFMTHRFGDFVLYEPPLNAKTYVLWLLPLLLLVVFCIMAVNSVRRNR

Specific function: Required for the biogenesis of c-type cytochromes. Possible subunit of a heme lyase [H]

COG id: COG3088

COG function: function code O; Uncharacterized protein involved in biosynthesis of c-type cytochromes

Gene ontology:

Cell location: Membrane; Single-pass membrane protein; Periplasmic side (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CcmH/CycL/Ccl2/NrfF family [H]

Homologues:

Organism=Escherichia coli, GI1788522, Length=99, Percent_Identity=39.3939393939394, Blast_Score=98, Evalue=2e-22,
Organism=Escherichia coli, GI1790512, Length=82, Percent_Identity=52.4390243902439, Blast_Score=97, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005616 [H]

Pfam domain/function: PF03918 CcmH [H]

EC number: NA

Molecular weight: Translated: 17180; Mature: 17180

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHSFKSLLYLFCLLSMFSVNAALDNSNDLFVSADKDVIESVELFEFTSQEQQKQAIELA
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHH
KRLRCPQCQNQNLIESNSPIAKDLRLTVYQQIKAGKSEEEVIAFMTHRFGDFVLYEPPLN
HHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCEEEECCCCC
AKTYVLWLLPLLLLVVFCIMAVNSVRRNR
CHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKHSFKSLLYLFCLLSMFSVNAALDNSNDLFVSADKDVIESVELFEFTSQEQQKQAIELA
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHH
KRLRCPQCQNQNLIESNSPIAKDLRLTVYQQIKAGKSEEEVIAFMTHRFGDFVLYEPPLN
HHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCEEEECCCCC
AKTYVLWLLPLLLLVVFCIMAVNSVRRNR
CHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]