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The map label for this gene is dcuA [H]

Identifier: 59712153

GI number: 59712153

Start: 1730066

End: 1731367

Strand: Direct

Name: dcuA [H]

Synonym: VF_1546

Alternate gene names: 59712153

Gene position: 1730066-1731367 (Clockwise)

Preceding gene: 59712151

Following gene: 59712154

Centisome position: 59.71

GC content: 42.78

Gene sequence:

>1302_bases
ATGTCGGTAATAATTGAAATCTCAGTAGTGCTAATTTGCATCTACTGGGGATTAAAAATGGGTGGCCTTGGCGTTGGTTT
GGCAGGAGGGTTTGGTCTTGCGGTATTAACGCTTGGATTAGGTCTCGAACCTAGCGCTCCTGCTATTGATGTTATTCTTA
TTATAATGTCTGTTGTGACTGCAGCTTCAACCATGCAGGCGGCGGGTGGTATGGAATGGCTAGTTCATCATGCAGGTAAC
TTAATGCGCCGCCATCCAAAATATATAAATATACTAGCACCGATCATTACTTGGTGTATGACCATTATGGCGGGCACAGG
GTATACGGTTTTTTCAACTCTTCCTGTTATTTCAGAAGTAGCCAAAGAAATTGGAGAAAGACCATCTCGCCCTTTATCTG
CGGCGGTTGTGGCTTCGCAAGTTGCAATTTCTGGCTCACCTATCTCTGCGGCAATGGCGGCCATGATTTCAATCATGGAA
CCTGCAGGTGTTAGCTTCTTACAAATTATGGCTATTTGCTTACCCGCTTCTTTCATTGCTGCAATGGTTGCGGCATTTAT
TGCCTCTCGACAAGGCTGTGAATTAAAAGACGACGTGGTTTATCAACAAATCCAAAAAGAAAATAAACAACATGTTACTT
CAATGACTAAAGACATTGATCCTAAGGCAAAATATTCAGTGTTTCTTTTTGCATTAGCTACGTGTGCCATTGTTCTTTAT
GCCGCTATTCCTTCACTACGTCCAGCCTTTCCAAGTGGCGATATCATGACAACACGTGACATCATTATTGTCAGTATGTT
TGCTGCAGCATGTTTGATGGTACCTATGTGTAATATTCATCCGGATCGTATTATTAAAACCACGACGTTCCGTTCAGGAA
TGAGCTCTGTAGCGGTTATTTTAGGTATTGTGACCATGGGCTCAACTGTTGTTAATGCTCACCTTCCTGAGATTAAAGAA
TTTGCGGGTTCTCTACTTCACTCTTACCCAATGTTGTTATCACTTGTTCTTTTTGGCACTTGCACATTATTAATGTCACA
AGGCGCAACAACACCACTGATTGTTCCTTTAGCGCTGAGCTTGAATGTTCCTCACTGGGCGATCTTAGCGTCATTCGTTG
CAGTAACTGGTGTGTTTGTTCTACCAACATACCCTACCTCTCTTGCAGCTATTGAGTTTGACTCAACAGGTTCAACTCGA
ATGGGGAAAAACATCTTTGATCATCCATTTATGCTACCGGGTCTAACAGGTGTCATTGTTGCCATTGGCTTTGGTTTCTT
ACTCGCTCCAGCAGTGCTTTAG

Upstream 100 bases:

>100_bases
CATTTAACAAAAACCATACCGACTGACATGATTTATTTAGCTTATCCGTCGCAAGATGAATTAAAAAAATCAATTAGTTA
AAATTTAAATAGGATGTTTT

Downstream 100 bases:

>100_bases
CACTTACTACAACGCGTATTTCATTTAAAGGAGTAAATATTATGAACAAGAAATTAGGTATTTTAGGCGGTATGGGACCT
TTAGCAACGGTTGAGTTTAT

Product: anaerobic C4-dicarboxylate transporter

Products: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MSVIIEISVVLICIYWGLKMGGLGVGLAGGFGLAVLTLGLGLEPSAPAIDVILIIMSVVTAASTMQAAGGMEWLVHHAGN
LMRRHPKYINILAPIITWCMTIMAGTGYTVFSTLPVISEVAKEIGERPSRPLSAAVVASQVAISGSPISAAMAAMISIME
PAGVSFLQIMAICLPASFIAAMVAAFIASRQGCELKDDVVYQQIQKENKQHVTSMTKDIDPKAKYSVFLFALATCAIVLY
AAIPSLRPAFPSGDIMTTRDIIIVSMFAAACLMVPMCNIHPDRIIKTTTFRSGMSSVAVILGIVTMGSTVVNAHLPEIKE
FAGSLLHSYPMLLSLVLFGTCTLLMSQGATTPLIVPLALSLNVPHWAILASFVAVTGVFVLPTYPTSLAAIEFDSTGSTR
MGKNIFDHPFMLPGLTGVIVAIGFGFLLAPAVL

Sequences:

>Translated_433_residues
MSVIIEISVVLICIYWGLKMGGLGVGLAGGFGLAVLTLGLGLEPSAPAIDVILIIMSVVTAASTMQAAGGMEWLVHHAGN
LMRRHPKYINILAPIITWCMTIMAGTGYTVFSTLPVISEVAKEIGERPSRPLSAAVVASQVAISGSPISAAMAAMISIME
PAGVSFLQIMAICLPASFIAAMVAAFIASRQGCELKDDVVYQQIQKENKQHVTSMTKDIDPKAKYSVFLFALATCAIVLY
AAIPSLRPAFPSGDIMTTRDIIIVSMFAAACLMVPMCNIHPDRIIKTTTFRSGMSSVAVILGIVTMGSTVVNAHLPEIKE
FAGSLLHSYPMLLSLVLFGTCTLLMSQGATTPLIVPLALSLNVPHWAILASFVAVTGVFVLPTYPTSLAAIEFDSTGSTR
MGKNIFDHPFMLPGLTGVIVAIGFGFLLAPAVL
>Mature_432_residues
SVIIEISVVLICIYWGLKMGGLGVGLAGGFGLAVLTLGLGLEPSAPAIDVILIIMSVVTAASTMQAAGGMEWLVHHAGNL
MRRHPKYINILAPIITWCMTIMAGTGYTVFSTLPVISEVAKEIGERPSRPLSAAVVASQVAISGSPISAAMAAMISIMEP
AGVSFLQIMAICLPASFIAAMVAAFIASRQGCELKDDVVYQQIQKENKQHVTSMTKDIDPKAKYSVFLFALATCAIVLYA
AIPSLRPAFPSGDIMTTRDIIIVSMFAAACLMVPMCNIHPDRIIKTTTFRSGMSSVAVILGIVTMGSTVVNAHLPEIKEF
AGSLLHSYPMLLSLVLFGTCTLLMSQGATTPLIVPLALSLNVPHWAILASFVAVTGVFVLPTYPTSLAAIEFDSTGSTRM
GKNIFDHPFMLPGLTGVIVAIGFGFLLAPAVL

Specific function: Responsible for the transport of C4-dicarboxylates from the periplasm across the inner membrane [H]

COG id: COG2704

COG function: function code R; Anaerobic C4-dicarboxylate transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dcuA/dcuB transporter (TC 2.A.13.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790580, Length=428, Percent_Identity=43.2242990654206, Blast_Score=334, Evalue=7e-93,
Organism=Escherichia coli, GI1790565, Length=401, Percent_Identity=34.4139650872818, Blast_Score=234, Evalue=6e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004668 [H]

Pfam domain/function: PF03605 DcuA_DcuB [H]

EC number: NA

Molecular weight: Translated: 45674; Mature: 45543

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
7.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
7.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVIIEISVVLICIYWGLKMGGLGVGLAGGFGLAVLTLGLGLEPSAPAIDVILIIMSVVT
CCEEEHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
AASTMQAAGGMEWLVHHAGNLMRRHPKYINILAPIITWCMTIMAGTGYTVFSTLPVISEV
HHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
AKEIGERPSRPLSAAVVASQVAISGSPISAAMAAMISIMEPAGVSFLQIMAICLPASFIA
HHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
AMVAAFIASRQGCELKDDVVYQQIQKENKQHVTSMTKDIDPKAKYSVFLFALATCAIVLY
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
AAIPSLRPAFPSGDIMTTRDIIIVSMFAAACLMVPMCNIHPDRIIKTTTFRSGMSSVAVI
HHHCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCHHEEHHHHHHHHHHHHHH
LGIVTMGSTVVNAHLPEIKEFAGSLLHSYPMLLSLVLFGTCTLLMSQGATTPLIVPLALS
HHHHHHCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHEE
LNVPHWAILASFVAVTGVFVLPTYPTSLAAIEFDSTGSTRMGKNIFDHPFMLPGLTGVIV
CCCCHHHHHHHHHHHHHHHEECCCCCCEEEEEECCCCCCHHCCCCCCCCCHHCCHHHHHH
AIGFGFLLAPAVL
HHHHHHHHHHHCC
>Mature Secondary Structure 
SVIIEISVVLICIYWGLKMGGLGVGLAGGFGLAVLTLGLGLEPSAPAIDVILIIMSVVT
CEEEHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
AASTMQAAGGMEWLVHHAGNLMRRHPKYINILAPIITWCMTIMAGTGYTVFSTLPVISEV
HHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
AKEIGERPSRPLSAAVVASQVAISGSPISAAMAAMISIMEPAGVSFLQIMAICLPASFIA
HHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
AMVAAFIASRQGCELKDDVVYQQIQKENKQHVTSMTKDIDPKAKYSVFLFALATCAIVLY
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
AAIPSLRPAFPSGDIMTTRDIIIVSMFAAACLMVPMCNIHPDRIIKTTTFRSGMSSVAVI
HHHCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCHHEEHHHHHHHHHHHHHH
LGIVTMGSTVVNAHLPEIKEFAGSLLHSYPMLLSLVLFGTCTLLMSQGATTPLIVPLALS
HHHHHHCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHEE
LNVPHWAILASFVAVTGVFVLPTYPTSLAAIEFDSTGSTRMGKNIFDHPFMLPGLTGVIV
CCCCHHHHHHHHHHHHHHHEECCCCCCEEEEEECCCCCCHHCCCCCCCCCHHCCHHHHHH
AIGFGFLLAPAVL
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Specific reaction: C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] = C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11004174; 14500908; 8898907 [H]