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The map label for this gene is yjiE [H]

Identifier: 59712152

GI number: 59712152

Start: 1728856

End: 1729737

Strand: Reverse

Name: yjiE [H]

Synonym: VF_1545

Alternate gene names: 59712152

Gene position: 1729737-1728856 (Counterclockwise)

Preceding gene: 59712155

Following gene: 59712150

Centisome position: 59.7

GC content: 37.53

Gene sequence:

>882_bases
ATGAATGTAGAAAGTAAATGGCTAGAAGATTTTTTAGTGTTAGCAAAAGTGAAAAATTTCTCACAAGCTGCTGAATTAAG
AAATGTCACTCAGCCTGCATTCAGTCGAAGAATTCGTTTATTAGAAGATACCGTAGGTGCTGAACTAGTCGATAGAAAGA
GTAAACCGATAGAGTTAACTCCAAGTGGTAAATTATTCCGAATAACCGCAAGAACCTTGGTCAATCAGATTGAAGCTGGC
ATAAGCCAAATATCAGATTTATCTCAATTAGGTGGTAATGTTGTTCAAGTTGCAGCGGCACACTCGCTAGCAACTAGTTT
AATACCTAAGATGCAGCAAGCGTTTGATGAGGGGGACTATAAACCGATTTTAAGTGTTGAAGCCATTGACGTAGACGAAG
CAACAAAAGAGTTAAGAGAGGGGGCATGTGACATATTATTAGCGTTTGATGATGATATTTTACGTTTGCCACCTTATCAG
TCTCAGCTTATTGCTAAAACAGAGCTGTTACCTGTGAGTGCATGTGATGAAATGGGAAAACCTATTTATGATTTTATATC
TCAAGGCGCTGTGCCTTGGTTAACATATAGTTCAACATCTTACATGGGAAGACAGGTAGAAATTATAAGAGAACAAGTGG
CGTTAACACCTATTTTTTCGTCATCAATGACTGATATGTTGAAGATATTAGTACTGAATAAACAAGGAATAGCTTGGTTG
CCTGCTTACAGTATTCAAGAAGAGTTAGCACAGAAAAAAGTCGCAATAATCGGTGAACAATCACTGCGACTTCCTATTGA
ATATTATGCGTATCGTTATCAAGCAAGGTTACATCCAGCCGGTGAAAAGGTATGGAGTATTCTATGTAATTTAGATACTT
AG

Upstream 100 bases:

>100_bases
TATTTTGGCTTGGCCGACATAAAATTTTTTAAAAAATGGTGATAATGCAGTTTCGGAATAGTGATAACTATCTCAAATAG
AATGGTTGTATGAGGTTGAA

Downstream 100 bases:

>100_bases
CTTACGGCATTATTTGCTTGATGATTCTTTTCAAAATTAATTTTTGGTACACAAAAGATTAATAATGAAGCTAAAATTGT
TGAGCCCATTAAATCTGTCG

Product: DNA-binding transcriptional regulator

Products: NA

Alternate protein names: Quorum-sensing regulator protein D [H]

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MNVESKWLEDFLVLAKVKNFSQAAELRNVTQPAFSRRIRLLEDTVGAELVDRKSKPIELTPSGKLFRITARTLVNQIEAG
ISQISDLSQLGGNVVQVAAAHSLATSLIPKMQQAFDEGDYKPILSVEAIDVDEATKELREGACDILLAFDDDILRLPPYQ
SQLIAKTELLPVSACDEMGKPIYDFISQGAVPWLTYSSTSYMGRQVEIIREQVALTPIFSSSMTDMLKILVLNKQGIAWL
PAYSIQEELAQKKVAIIGEQSLRLPIEYYAYRYQARLHPAGEKVWSILCNLDT

Sequences:

>Translated_293_residues
MNVESKWLEDFLVLAKVKNFSQAAELRNVTQPAFSRRIRLLEDTVGAELVDRKSKPIELTPSGKLFRITARTLVNQIEAG
ISQISDLSQLGGNVVQVAAAHSLATSLIPKMQQAFDEGDYKPILSVEAIDVDEATKELREGACDILLAFDDDILRLPPYQ
SQLIAKTELLPVSACDEMGKPIYDFISQGAVPWLTYSSTSYMGRQVEIIREQVALTPIFSSSMTDMLKILVLNKQGIAWL
PAYSIQEELAQKKVAIIGEQSLRLPIEYYAYRYQARLHPAGEKVWSILCNLDT
>Mature_293_residues
MNVESKWLEDFLVLAKVKNFSQAAELRNVTQPAFSRRIRLLEDTVGAELVDRKSKPIELTPSGKLFRITARTLVNQIEAG
ISQISDLSQLGGNVVQVAAAHSLATSLIPKMQQAFDEGDYKPILSVEAIDVDEATKELREGACDILLAFDDDILRLPPYQ
SQLIAKTELLPVSACDEMGKPIYDFISQGAVPWLTYSSTSYMGRQVEIIREQVALTPIFSSSMTDMLKILVLNKQGIAWL
PAYSIQEELAQKKVAIIGEQSLRLPIEYYAYRYQARLHPAGEKVWSILCNLDT

Specific function: Down-regulates expression of the flagellar regulon, decreasing motility [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790783, Length=296, Percent_Identity=40.2027027027027, Blast_Score=233, Evalue=1e-62,
Organism=Escherichia coli, GI145693105, Length=117, Percent_Identity=29.0598290598291, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI157672245, Length=111, Percent_Identity=30.6306306306306, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32736; Mature: 32736

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVESKWLEDFLVLAKVKNFSQAAELRNVTQPAFSRRIRLLEDTVGAELVDRKSKPIELT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEC
PSGKLFRITARTLVNQIEAGISQISDLSQLGGNVVQVAAAHSLATSLIPKMQQAFDEGDY
CCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCHHEEHHHHHHHHHHHHHHHHHHHCCCCC
KPILSVEAIDVDEATKELREGACDILLAFDDDILRLPPYQSQLIAKTELLPVSACDEMGK
CCEEEEEEECHHHHHHHHHCCCCEEEEEECCCCEECCCCHHHHHHHHHCCCHHHHHHHCC
PIYDFISQGAVPWLTYSSTSYMGRQVEIIREQVALTPIFSSSMTDMLKILVLNKQGIAWL
HHHHHHHCCCCCEEEECCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEC
PAYSIQEELAQKKVAIIGEQSLRLPIEYYAYRYQARLHPAGEKVWSILCNLDT
CCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MNVESKWLEDFLVLAKVKNFSQAAELRNVTQPAFSRRIRLLEDTVGAELVDRKSKPIELT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEC
PSGKLFRITARTLVNQIEAGISQISDLSQLGGNVVQVAAAHSLATSLIPKMQQAFDEGDY
CCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCHHEEHHHHHHHHHHHHHHHHHHHCCCCC
KPILSVEAIDVDEATKELREGACDILLAFDDDILRLPPYQSQLIAKTELLPVSACDEMGK
CCEEEEEEECHHHHHHHHHCCCCEEEEEECCCCEECCCCHHHHHHHHHCCCHHHHHHHCC
PIYDFISQGAVPWLTYSSTSYMGRQVEIIREQVALTPIFSSSMTDMLKILVLNKQGIAWL
HHHHHHHCCCCCEEEECCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEC
PAYSIQEELAQKKVAIIGEQSLRLPIEYYAYRYQARLHPAGEKVWSILCNLDT
CCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 7876157 [H]