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The map label for this gene is miaE [H]

Identifier: 59712099

GI number: 59712099

Start: 1651575

End: 1652333

Strand: Reverse

Name: miaE [H]

Synonym: VF_1492

Alternate gene names: 59712099

Gene position: 1652333-1651575 (Counterclockwise)

Preceding gene: 59712101

Following gene: 59712097

Centisome position: 57.03

GC content: 38.08

Gene sequence:

>759_bases
ATGTACGACAAATCTTTATTAGACCCAATTTATTCTTTTCTGCAGTGTGAAACGCCTGACGAATGGATAGAAGAAGCAAG
AAAGCCTGAAAACTTATCAGTTATTTTGCGTGATCACTTAATGTGTGAATTAAAAGCGGCACAGAGTGCAATGTATTTAA
TCCGCAAATACGCTGTTGATGCTGAAGGAGCAAAGGCATTACTGGATTGGTTCAAACCGTATGAAGAATTTGCCTATAAA
CATGTAGGGTCATTAGAGACGCTAAAGGGTAAAAGTAACATCTCTAAAAAGATCACTGCACGTGCTGATTCACCTTATAG
CCAAGATCTGATTGATAAAATGGTGTTATTGATAAAAGAAGAGCTTCATCATTTTTATCAAGTATTAGAAATTATGGATC
AGCGTGGTGTGCCTTATCAAAATATTAGAGCAGGGCGCTATGCAAAAGGGTTATTTACGAGAGTGATTACTCATGAGCCA
TGTACCTTAATTGATAAGTTGATCATTGGTGCTTTTATTGAGGCTCGTTCTTGTGAGCGCTTTGCTAAGTTAGCTCCGTT
TTTAGATGATGATTTGGCTAAGTTCTACATTTCATTACTTCGATCTGAATCTCGTCATTATCAAGATTACTTAGCACTAG
CACAGCAGGTTGCTGGGGGGAATGTGGATGAGCGTGTTGCATTTTTTGCTCAGGTTGAAGCGGATTTGATATTAACACCA
GACAGTGATTTTAAATTCCACAGTGGTGTTCCTGTTTAA

Upstream 100 bases:

>100_bases
GTTATTGAAAGAAAAGGACTGTTGATTTTTACCTTACGTAATGAATTTATATGGTGAGGTTTGGTATACTGGCGATAAGA
ACTTTTAATGGTTAAAAACT

Downstream 100 bases:

>100_bases
GTGTTATTAAGACAGACATAAAAAAGATCGCATTATGCGATCTTTTTTTTGGTAGGTATTGGTTACCATTTTTTCTTTGG
CTGGAAAAGAACATCGATAT

Product: tRNA-(MS(2)IO(6)A)-hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MYDKSLLDPIYSFLQCETPDEWIEEARKPENLSVILRDHLMCELKAAQSAMYLIRKYAVDAEGAKALLDWFKPYEEFAYK
HVGSLETLKGKSNISKKITARADSPYSQDLIDKMVLLIKEELHHFYQVLEIMDQRGVPYQNIRAGRYAKGLFTRVITHEP
CTLIDKLIIGAFIEARSCERFAKLAPFLDDDLAKFYISLLRSESRHYQDYLALAQQVAGGNVDERVAFFAQVEADLILTP
DSDFKFHSGVPV

Sequences:

>Translated_252_residues
MYDKSLLDPIYSFLQCETPDEWIEEARKPENLSVILRDHLMCELKAAQSAMYLIRKYAVDAEGAKALLDWFKPYEEFAYK
HVGSLETLKGKSNISKKITARADSPYSQDLIDKMVLLIKEELHHFYQVLEIMDQRGVPYQNIRAGRYAKGLFTRVITHEP
CTLIDKLIIGAFIEARSCERFAKLAPFLDDDLAKFYISLLRSESRHYQDYLALAQQVAGGNVDERVAFFAQVEADLILTP
DSDFKFHSGVPV
>Mature_252_residues
MYDKSLLDPIYSFLQCETPDEWIEEARKPENLSVILRDHLMCELKAAQSAMYLIRKYAVDAEGAKALLDWFKPYEEFAYK
HVGSLETLKGKSNISKKITARADSPYSQDLIDKMVLLIKEELHHFYQVLEIMDQRGVPYQNIRAGRYAKGLFTRVITHEP
CTLIDKLIIGAFIEARSCERFAKLAPFLDDDLAKFYISLLRSESRHYQDYLALAQQVAGGNVDERVAFFAQVEADLILTP
DSDFKFHSGVPV

Specific function: Production of the modified nucleoside 2-methylthio-cis- ribozeatin (MS[2]IO[6]A) found in some tRNAs. Catalyzes the oxygen-dependent transformation of MS[2]I[6]A into MS[2]IO[6]A [H]

COG id: COG4445

COG function: function code FJ; Hydroxylase for synthesis of 2-methylthio-cis-ribozeatin in tRNA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012347
- InterPro:   IPR010386 [H]

Pfam domain/function: PF06175 MiaE [H]

EC number: NA

Molecular weight: Translated: 28925; Mature: 28925

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYDKSLLDPIYSFLQCETPDEWIEEARKPENLSVILRDHLMCELKAAQSAMYLIRKYAVD
CCCHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
AEGAKALLDWFKPYEEFAYKHVGSLETLKGKSNISKKITARADSPYSQDLIDKMVLLIKE
CCHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
ELHHFYQVLEIMDQRGVPYQNIRAGRYAKGLFTRVITHEPCTLIDKLIIGAFIEARSCER
HHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
FAKLAPFLDDDLAKFYISLLRSESRHYQDYLALAQQVAGGNVDERVAFFAQVEADLILTP
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEECC
DSDFKFHSGVPV
CCCCCCCCCCCC
>Mature Secondary Structure
MYDKSLLDPIYSFLQCETPDEWIEEARKPENLSVILRDHLMCELKAAQSAMYLIRKYAVD
CCCHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
AEGAKALLDWFKPYEEFAYKHVGSLETLKGKSNISKKITARADSPYSQDLIDKMVLLIKE
CCHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
ELHHFYQVLEIMDQRGVPYQNIRAGRYAKGLFTRVITHEPCTLIDKLIIGAFIEARSCER
HHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
FAKLAPFLDDDLAKFYISLLRSESRHYQDYLALAQQVAGGNVDERVAFFAQVEADLILTP
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEECC
DSDFKFHSGVPV
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8253666; 11677609 [H]