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The map label for this gene is cysB [H]

Identifier: 59712097

GI number: 59712097

Start: 1649491

End: 1650465

Strand: Reverse

Name: cysB [H]

Synonym: VF_1490

Alternate gene names: 59712097

Gene position: 1650465-1649491 (Counterclockwise)

Preceding gene: 59712099

Following gene: 59712096

Centisome position: 56.96

GC content: 40.62

Gene sequence:

>975_bases
ATGAAATTACAGCAATTAAAGTACATTGTTGAAGTTGTGAATCACAGTTTGAATGTATCATCGACTGCTGAGTCGCTATA
CACCTCACAACCGGGTATTAGTAAACAAGTTCGATTATTAGAAGATGAGCTTGGTGTTCAGATTTTTGAACGCAGTGGTA
AACATTTAACAAGAGTAACGCCATCAGGTGAAGAGATCGTTAAAATCTCACGCGAAATCTTATCTCGAGTTGAGAGCATT
AAATCGGTTGCTGGTGAGCATACGCACCCAGAAAAAGGAACTTTAAATATAACTACTACGCATACCCAAGCTCGTTATGC
GTTACCAGACGTAATTAAAGGCTTTAAGAATCGATACCCAGCGGTGTCATTGCATATGCACCAAGGCACGCCAAGCCAGA
TGTCCGATGCAATAGCCAAAGGTACGGCCGATTTTGCTATAGCAACCGAAGCGCTTCATTTATACCAAGATGCGATCATG
CTTCCTTGTTATCATTGGAATCGTTCTATTGTGGTAACAAAAGATCACCCATTAGCACAACTACAGCAAGTGACGATTGA
AGATCTTGCCGCTTTTGAATTAGTTACTTACGTATTTGGCTTTACGGGTCGATCGGCATTGGATAGTGCTTTTAATAAAG
CAGGCCTAAAACCGAAAATTGTCTTTACCGCAACCGATGCAGATGTCATTAAGACTTATGTTCGCTTAGGGATTGGTGTT
GGTGTAATAGCAAGCATGGCAATGGATGAAGAGCGAGATTCTGATCTTGTAGCCATTGATGCAAGCCATATTTTCGATGC
GAGTACCACAACCATTGGCTTTAAGCGTGGTTCATTCTTAAAAAGCTACATGTTTGATTTTATGGAACGCTTTGCGCCGC
ATTTAACACGTCCAATTGTTGAACAAGCAACGATGCTGAAAAATAATCACGAAATTGAAGAGATGTTTAAAGACATAAAC
CTTCCTGTCCGTTAA

Upstream 100 bases:

>100_bases
TATAATTAATAAACATAACACTTTGAAAATAGGTAGGAATAGGTATATAACTGTATCGTATAAGTAGAATTTAATGTAAA
GGATTACAGGAAATCATAAA

Downstream 100 bases:

>100_bases
GATCGCCCTGATATTATTTTCAGGGGCATATAATGCATTCTCAGCAGTTTTTAAATATAGACCGTTTGTTATCGCAGACG
GTCTTTTTTTGGCAATTTTC

Product: transcriptional regulator CysB

Products: NA

Alternate protein names: Cys regulon transcriptional activator [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MKLQQLKYIVEVVNHSLNVSSTAESLYTSQPGISKQVRLLEDELGVQIFERSGKHLTRVTPSGEEIVKISREILSRVESI
KSVAGEHTHPEKGTLNITTTHTQARYALPDVIKGFKNRYPAVSLHMHQGTPSQMSDAIAKGTADFAIATEALHLYQDAIM
LPCYHWNRSIVVTKDHPLAQLQQVTIEDLAAFELVTYVFGFTGRSALDSAFNKAGLKPKIVFTATDADVIKTYVRLGIGV
GVIASMAMDEERDSDLVAIDASHIFDASTTTIGFKRGSFLKSYMFDFMERFAPHLTRPIVEQATMLKNNHEIEEMFKDIN
LPVR

Sequences:

>Translated_324_residues
MKLQQLKYIVEVVNHSLNVSSTAESLYTSQPGISKQVRLLEDELGVQIFERSGKHLTRVTPSGEEIVKISREILSRVESI
KSVAGEHTHPEKGTLNITTTHTQARYALPDVIKGFKNRYPAVSLHMHQGTPSQMSDAIAKGTADFAIATEALHLYQDAIM
LPCYHWNRSIVVTKDHPLAQLQQVTIEDLAAFELVTYVFGFTGRSALDSAFNKAGLKPKIVFTATDADVIKTYVRLGIGV
GVIASMAMDEERDSDLVAIDASHIFDASTTTIGFKRGSFLKSYMFDFMERFAPHLTRPIVEQATMLKNNHEIEEMFKDIN
LPVR
>Mature_324_residues
MKLQQLKYIVEVVNHSLNVSSTAESLYTSQPGISKQVRLLEDELGVQIFERSGKHLTRVTPSGEEIVKISREILSRVESI
KSVAGEHTHPEKGTLNITTTHTQARYALPDVIKGFKNRYPAVSLHMHQGTPSQMSDAIAKGTADFAIATEALHLYQDAIM
LPCYHWNRSIVVTKDHPLAQLQQVTIEDLAAFELVTYVFGFTGRSALDSAFNKAGLKPKIVFTATDADVIKTYVRLGIGV
GVIASMAMDEERDSDLVAIDASHIFDASTTTIGFKRGSFLKSYMFDFMERFAPHLTRPIVEQATMLKNNHEIEEMFKDIN
LPVR

Specific function: This protein is a positive regulator of gene expression for the cysteine regulon. The inducer for cysB is N-acetylserine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787530, Length=322, Percent_Identity=74.2236024844721, Blast_Score=518, Evalue=1e-148,
Organism=Escherichia coli, GI1788296, Length=313, Percent_Identity=40.2555910543131, Blast_Score=259, Evalue=2e-70,
Organism=Escherichia coli, GI1790399, Length=287, Percent_Identity=27.8745644599303, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI157672245, Length=235, Percent_Identity=28.0851063829787, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1788887, Length=246, Percent_Identity=28.4552845528455, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI145693105, Length=271, Percent_Identity=21.4022140221402, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1790262, Length=220, Percent_Identity=24.5454545454545, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787879, Length=251, Percent_Identity=25.8964143426295, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1788748, Length=258, Percent_Identity=22.8682170542636, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1788481, Length=251, Percent_Identity=24.3027888446215, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789204, Length=240, Percent_Identity=23.3333333333333, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787601, Length=149, Percent_Identity=27.5167785234899, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 36139; Mature: 36139

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLQQLKYIVEVVNHSLNVSSTAESLYTSQPGISKQVRLLEDELGVQIFERSGKHLTRVT
CCHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEC
PSGEEIVKISREILSRVESIKSVAGEHTHPEKGTLNITTTHTQARYALPDVIKGFKNRYP
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCC
AVSLHMHQGTPSQMSDAIAKGTADFAIATEALHLYQDAIMLPCYHWNRSIVVTKDHPLAQ
EEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCEEECCCEEEEECCCCHHH
LQQVTIEDLAAFELVTYVFGFTGRSALDSAFNKAGLKPKIVFTATDADVIKTYVRLGIGV
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHH
GVIASMAMDEERDSDLVAIDASHIFDASTTTIGFKRGSFLKSYMFDFMERFAPHLTRPIV
HHHHHHHHCCCCCCCEEEEEHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
EQATMLKNNHEIEEMFKDINLPVR
HHHHHHHCCCHHHHHHHHCCCCCC
>Mature Secondary Structure
MKLQQLKYIVEVVNHSLNVSSTAESLYTSQPGISKQVRLLEDELGVQIFERSGKHLTRVT
CCHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEC
PSGEEIVKISREILSRVESIKSVAGEHTHPEKGTLNITTTHTQARYALPDVIKGFKNRYP
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCC
AVSLHMHQGTPSQMSDAIAKGTADFAIATEALHLYQDAIMLPCYHWNRSIVVTKDHPLAQ
EEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCEEECCCEEEEECCCCHHH
LQQVTIEDLAAFELVTYVFGFTGRSALDSAFNKAGLKPKIVFTATDADVIKTYVRLGIGV
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHH
GVIASMAMDEERDSDLVAIDASHIFDASTTTIGFKRGSFLKSYMFDFMERFAPHLTRPIV
HHHHHHHHCCCCCCCEEEEEHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
EQATMLKNNHEIEEMFKDINLPVR
HHHHHHHCCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]