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The map label for this gene is rpfG [H]

Identifier: 59711974

GI number: 59711974

Start: 1511244

End: 1513337

Strand: Reverse

Name: rpfG [H]

Synonym: VF_1367

Alternate gene names: 59711974

Gene position: 1513337-1511244 (Counterclockwise)

Preceding gene: 59711975

Following gene: 59711972

Centisome position: 52.23

GC content: 32.52

Gene sequence:

>2094_bases
ATGTGTGTTTTTTCTTCATTTGTTTCTTCAATGGATTTAACAGAGGAAGAGCAAGTATGGATACAAAACAATAAAAATTT
TGTGTTTTATAACAATGATTATAATTCAAGATTATTTTTTATTGAGGATGGGGCACCAAAAGGACTGTACAGCAACCTAA
TTCAAGATATTAACAAAAAACTCAATACAAACTTCCAACTAAAAGTAAAAGACATTGATGATTTACATGCAATGTTTGAC
CGTTTAGAGAGTGGGGCTTATTTAGATTTTGCTGATACTGAAAACAGAAAAGAAAACTATTTTTTTGTCCCAACATTGTA
TCGTGTAAGCACCAAAGTTTTTTATAAAAATACGGATCAAATTATTGATCTTGTAAGTTTAAATAATAAGAAAATTGGCT
TAGTTGAAGATTGGTTTTCTACGACGAATTTTATTGAACAATACTCTGATTCTATACAATATACACCAGTAAAATTTGCG
GATGTTAATTCACTGATAAACGCTCTTAATTCCGGAGAGATTGACGCTTTTGTTGCTGATACACAAGAAATAACGGACAA
AGCATTTCCGACATTAAACTTACCAAGATTGAATAACCTATATACCTCATTTGCAATACCAAAATCGCAATCTGAATTGT
ATAGCGTATTAGTAAAATACTTTATTGAGTTGAAATCAGAAGAAGTAAAAGCGTTTGTTAAAAAATCCCGTGAAGATTAC
TTTTTGTATCTTTTTAAAGGAAGTAGTGAACTTAAAGGATCAAGCGTCAGTGTTGGTTATGGCGTTAATGAGTTTCCTGC
AAGCTACTTAGTTAATGAAGAGTATCACGGCATTGCACCTACGTTGTTCGACAGTATTAAGCGAATATTTAAAGATTCGG
TCACCTTTACTGAGCCAAGTGTTGGTAAGTGTAAAGATTATGATGTTTTATTATCAACATTTGAAAATGAGTGCATCACA
GATAAATATCTTCTAACAAAACCGTATTACAGTTTTGAATTGTCAGTATTTAATAAGTTAGAAGGGAACTTTATATCTCG
TATTGCAGATATAAACTACTCGACGGTAGGGATGCTGAAAAACGCTTATTATTACGATTATATAAAAAACAATACGTTAA
ATGTGAAAATGGTCTTTTTTGATACCTTTGAAGAGATCATTGAAGCAATAGATGACGGACGAGTTGATTATGCTTTTGGT
GACCAACGTTTATTAATGAATCATACAATCAACCATGACATGTATGATTTAAAAACCGCAGGAACCTTAACCAAAACATT
CTCTGTGTATATCGCAGTAAAGAAAGAGCATGAAGCACTATTTAATGCCATTAATCTGATATCAACATCATCAGATAATG
AACGTTTGATTAAAAACATCTATATCAATGAGAATGAAAAGTATCAGAAAGATGATTTCTGGCTACTAATCGCAGTTCTA
CTTGTCAGCTTATTTATCATTGGCGTGTTGATAACTCGAGTTTATATAGAGAAAAAAGCACAAGCTCGTCTTTTAAGAAT
GAACGAATCATTGATTGGTTCATTGGAAATGGCCTCATTATACAGTGATGAAGAAACCAGTGAGCATAACAAGCGAATCA
ACGTATATTCTGAGCATCTTTCTAATTTATTGAATATGCCAAACTCGTTTGTAGAAGAAATTCGTATGATTGCTTCGCTG
CATGACATTGGAAAGATTGGTATACCTCATTACATATTGAAGAAACCTGGAAAATTGGATCCTGATGAATTCGACGTAAT
GAAAAAACACGTAAACATTGGTTATGAGATTATCAAGAATACAGACTTGAGTTTGATAACTAAAAATATCGTGCTTTACC
ATCATGAAAAATGGAATGGTAAAGGATACTTGAGTTTAGTAGGTGAAGAGATTCCGATTGAAGCTCGAATTGTAAGTATT
GTGGATGTGTATGACGCATTACGACAAAAGCGTATTTACAAAGAAGGCTTTACTCATGAAGTGGCTATTAAGATCATCGA
AGAAGAAAAGGGCGTTTCTTTCGACCCTAAGATTGTCGATATATTCTTAGAGCATCATGAAGAGTTCAGAGCTATTTTTG
AGAATAATCAGTAA

Upstream 100 bases:

>100_bases
TACATTTGCAGAGTGCTTTGTTAGTGTGCGTTTTTTGCACTTATTTGTGTTTATTGATTGTTATGGTGTATGCAAATTGA
AACGAATTTTCGTTTTTATC

Downstream 100 bases:

>100_bases
GTGAGTAAGAGCAGGGACTAGTTTTTAGGGACTAGGGATTAGTAAGTCTTGAGGGACTAGTGGCGAGTGAGAGCAGGGAC
GAGTAACTGCTTTTCGCTCT

Product: two-component response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 697; Mature: 697

Protein sequence:

>697_residues
MCVFSSFVSSMDLTEEEQVWIQNNKNFVFYNNDYNSRLFFIEDGAPKGLYSNLIQDINKKLNTNFQLKVKDIDDLHAMFD
RLESGAYLDFADTENRKENYFFVPTLYRVSTKVFYKNTDQIIDLVSLNNKKIGLVEDWFSTTNFIEQYSDSIQYTPVKFA
DVNSLINALNSGEIDAFVADTQEITDKAFPTLNLPRLNNLYTSFAIPKSQSELYSVLVKYFIELKSEEVKAFVKKSREDY
FLYLFKGSSELKGSSVSVGYGVNEFPASYLVNEEYHGIAPTLFDSIKRIFKDSVTFTEPSVGKCKDYDVLLSTFENECIT
DKYLLTKPYYSFELSVFNKLEGNFISRIADINYSTVGMLKNAYYYDYIKNNTLNVKMVFFDTFEEIIEAIDDGRVDYAFG
DQRLLMNHTINHDMYDLKTAGTLTKTFSVYIAVKKEHEALFNAINLISTSSDNERLIKNIYINENEKYQKDDFWLLIAVL
LVSLFIIGVLITRVYIEKKAQARLLRMNESLIGSLEMASLYSDEETSEHNKRINVYSEHLSNLLNMPNSFVEEIRMIASL
HDIGKIGIPHYILKKPGKLDPDEFDVMKKHVNIGYEIIKNTDLSLITKNIVLYHHEKWNGKGYLSLVGEEIPIEARIVSI
VDVYDALRQKRIYKEGFTHEVAIKIIEEEKGVSFDPKIVDIFLEHHEEFRAIFENNQ

Sequences:

>Translated_697_residues
MCVFSSFVSSMDLTEEEQVWIQNNKNFVFYNNDYNSRLFFIEDGAPKGLYSNLIQDINKKLNTNFQLKVKDIDDLHAMFD
RLESGAYLDFADTENRKENYFFVPTLYRVSTKVFYKNTDQIIDLVSLNNKKIGLVEDWFSTTNFIEQYSDSIQYTPVKFA
DVNSLINALNSGEIDAFVADTQEITDKAFPTLNLPRLNNLYTSFAIPKSQSELYSVLVKYFIELKSEEVKAFVKKSREDY
FLYLFKGSSELKGSSVSVGYGVNEFPASYLVNEEYHGIAPTLFDSIKRIFKDSVTFTEPSVGKCKDYDVLLSTFENECIT
DKYLLTKPYYSFELSVFNKLEGNFISRIADINYSTVGMLKNAYYYDYIKNNTLNVKMVFFDTFEEIIEAIDDGRVDYAFG
DQRLLMNHTINHDMYDLKTAGTLTKTFSVYIAVKKEHEALFNAINLISTSSDNERLIKNIYINENEKYQKDDFWLLIAVL
LVSLFIIGVLITRVYIEKKAQARLLRMNESLIGSLEMASLYSDEETSEHNKRINVYSEHLSNLLNMPNSFVEEIRMIASL
HDIGKIGIPHYILKKPGKLDPDEFDVMKKHVNIGYEIIKNTDLSLITKNIVLYHHEKWNGKGYLSLVGEEIPIEARIVSI
VDVYDALRQKRIYKEGFTHEVAIKIIEEEKGVSFDPKIVDIFLEHHEEFRAIFENNQ
>Mature_697_residues
MCVFSSFVSSMDLTEEEQVWIQNNKNFVFYNNDYNSRLFFIEDGAPKGLYSNLIQDINKKLNTNFQLKVKDIDDLHAMFD
RLESGAYLDFADTENRKENYFFVPTLYRVSTKVFYKNTDQIIDLVSLNNKKIGLVEDWFSTTNFIEQYSDSIQYTPVKFA
DVNSLINALNSGEIDAFVADTQEITDKAFPTLNLPRLNNLYTSFAIPKSQSELYSVLVKYFIELKSEEVKAFVKKSREDY
FLYLFKGSSELKGSSVSVGYGVNEFPASYLVNEEYHGIAPTLFDSIKRIFKDSVTFTEPSVGKCKDYDVLLSTFENECIT
DKYLLTKPYYSFELSVFNKLEGNFISRIADINYSTVGMLKNAYYYDYIKNNTLNVKMVFFDTFEEIIEAIDDGRVDYAFG
DQRLLMNHTINHDMYDLKTAGTLTKTFSVYIAVKKEHEALFNAINLISTSSDNERLIKNIYINENEKYQKDDFWLLIAVL
LVSLFIIGVLITRVYIEKKAQARLLRMNESLIGSLEMASLYSDEETSEHNKRINVYSEHLSNLLNMPNSFVEEIRMIASL
HDIGKIGIPHYILKKPGKLDPDEFDVMKKHVNIGYEIIKNTDLSLITKNIVLYHHEKWNGKGYLSLVGEEIPIEARIVSI
VDVYDALRQKRIYKEGFTHEVAIKIIEEEKGVSFDPKIVDIFLEHHEEFRAIFENNQ

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 80772; Mature: 80772

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCVFSSFVSSMDLTEEEQVWIQNNKNFVFYNNDYNSRLFFIEDGAPKGLYSNLIQDINKK
CCCHHHHHHHCCCCCCCCEEEECCCEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHH
LNTNFQLKVKDIDDLHAMFDRLESGAYLDFADTENRKENYFFVPTLYRVSTKVFYKNTDQ
HCCCEEEEECCHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEEHHHHEEEEEECCHHH
IIDLVSLNNKKIGLVEDWFSTTNFIEQYSDSIQYTPVKFADVNSLINALNSGEIDAFVAD
HEEHEECCCCCCCCHHHHHHHHHHHHHHCCCCEECCEEHHHHHHHHHHCCCCCCEEEECC
TQEITDKAFPTLNLPRLNNLYTSFAIPKSQSELYSVLVKYFIELKSEEVKAFVKKSREDY
HHHHHHHCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
FLYLFKGSSELKGSSVSVGYGVNEFPASYLVNEEYHGIAPTLFDSIKRIFKDSVTFTEPS
EEEEEECCCCCCCCEEEECCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCC
VGKCKDYDVLLSTFENECITDKYLLTKPYYSFELSVFNKLEGNFISRIADINYSTVGMLK
CCCCCCHHHHHHHHCCCCCCCCEEEECCCCEEEEHHHHHHCCHHHHHHHCCCCHHHHHHH
NAYYYDYIKNNTLNVKMVFFDTFEEIIEAIDDGRVDYAFGDQRLLMNHTINHDMYDLKTA
CCEEEEEECCCEEEEEEEEHHHHHHHHHHHCCCCEEEEECCCEEEECCCCCCCCHHHHCC
GTLTKTFSVYIAVKKEHEALFNAINLISTSSDNERLIKNIYINENEKYQKDDFWLLIAVL
CCCEEEEEEEEEEEHHHHHHHHHHHHHCCCCCCHHEEHEEEECCCCCCCCCHHHHHHHHH
LVSLFIIGVLITRVYIEKKAQARLLRMNESLIGSLEMASLYSDEETSEHNKRINVYSEHL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCHHHHHHHCCCCCCCCCCEEHHHHHHH
SNLLNMPNSFVEEIRMIASLHDIGKIGIPHYILKKPGKLDPDEFDVMKKHVNIGYEIIKN
HHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCHHHHHHHHHHCCCEEEECC
TDLSLITKNIVLYHHEKWNGKGYLSLVGEEIPIEARIVSIVDVYDALRQKRIYKEGFTHE
CCHHHEEEEEEEEEECCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEE
VAIKIIEEEKGVSFDPKIVDIFLEHHEEFRAIFENNQ
EEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MCVFSSFVSSMDLTEEEQVWIQNNKNFVFYNNDYNSRLFFIEDGAPKGLYSNLIQDINKK
CCCHHHHHHHCCCCCCCCEEEECCCEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHH
LNTNFQLKVKDIDDLHAMFDRLESGAYLDFADTENRKENYFFVPTLYRVSTKVFYKNTDQ
HCCCEEEEECCHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEEHHHHEEEEEECCHHH
IIDLVSLNNKKIGLVEDWFSTTNFIEQYSDSIQYTPVKFADVNSLINALNSGEIDAFVAD
HEEHEECCCCCCCCHHHHHHHHHHHHHHCCCCEECCEEHHHHHHHHHHCCCCCCEEEECC
TQEITDKAFPTLNLPRLNNLYTSFAIPKSQSELYSVLVKYFIELKSEEVKAFVKKSREDY
HHHHHHHCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
FLYLFKGSSELKGSSVSVGYGVNEFPASYLVNEEYHGIAPTLFDSIKRIFKDSVTFTEPS
EEEEEECCCCCCCCEEEECCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCC
VGKCKDYDVLLSTFENECITDKYLLTKPYYSFELSVFNKLEGNFISRIADINYSTVGMLK
CCCCCCHHHHHHHHCCCCCCCCEEEECCCCEEEEHHHHHHCCHHHHHHHCCCCHHHHHHH
NAYYYDYIKNNTLNVKMVFFDTFEEIIEAIDDGRVDYAFGDQRLLMNHTINHDMYDLKTA
CCEEEEEECCCEEEEEEEEHHHHHHHHHHHCCCCEEEEECCCEEEECCCCCCCCHHHHCC
GTLTKTFSVYIAVKKEHEALFNAINLISTSSDNERLIKNIYINENEKYQKDDFWLLIAVL
CCCEEEEEEEEEEEHHHHHHHHHHHHHCCCCCCHHEEHEEEECCCCCCCCCHHHHHHHHH
LVSLFIIGVLITRVYIEKKAQARLLRMNESLIGSLEMASLYSDEETSEHNKRINVYSEHL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCHHHHHHHCCCCCCCCCCEEHHHHHHH
SNLLNMPNSFVEEIRMIASLHDIGKIGIPHYILKKPGKLDPDEFDVMKKHVNIGYEIIKN
HHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCHHHHHHHHHHCCCEEEECC
TDLSLITKNIVLYHHEKWNGKGYLSLVGEEIPIEARIVSIVDVYDALRQKRIYKEGFTHE
CCHHHEEEEEEEEEECCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEE
VAIKIIEEEKGVSFDPKIVDIFLEHHEEFRAIFENNQ
EEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]