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The map label for this gene is galR [H]

Identifier: 59711862

GI number: 59711862

Start: 1396688

End: 1397710

Strand: Reverse

Name: galR [H]

Synonym: VF_1255

Alternate gene names: 59711862

Gene position: 1397710-1396688 (Counterclockwise)

Preceding gene: 59711864

Following gene: 59711861

Centisome position: 48.24

GC content: 42.23

Gene sequence:

>1023_bases
ATGGCGACAATCAGTGACGTTGCAAAGTTAGCAAGAGTTTCTACCGCTACCGTTTCCCGCGTAATCAATAACTCCGCTTA
TGTTGAGCCGGTAACTCTTGAACGAGTACAAAGTGCCATTCGTGAACTCAATTATCAGCGCGATGCAAGAGCCAGTGCCT
TAGCAAAAAAAACAAATAATACCCTTGGCCTACTAACCGGCAACCTAGCTGATCCTTTCTTTGCCCTAATAGCCAAACAC
GTAGAAGAAGTCGCACGTAAACATAAATGCCAATTAGTAGTAGTAAGCGGAGGTCATGATGCACAGCGAGAAAAAGAAGG
GTTGGATTTTTTAATCAGTCAAGGATGTGAAGCCATGGTGGTTCACGCCAAAATGCTAGATGATGCCACCCTATTACGAT
ACAGCGCTCAACTCCCTTCGATGGTATTGCTCAACCGTACCATTCCACAAATCTCAAATCGTTCTGTCTATATTGATAAT
AGAGCTGGTGCCAAAGAATCTGTTGTTTACTTAATTAAGCAAGGTCACCGCAAAATCGCCTGTGTCACCAGTGATCTTCC
TATTGAAGACAGAACAGAGCGACTTAACGGTTACCGCGATGCTATGGCAGAAGCCAACATTACCATTGAACCTAACTGGA
TAATAAATCAGAACTTTAGCGAACAAGGCGGAGAACTTGCCGGTGAACAATTATTGTTTCAATGCCCAGAAGTCACTGCT
GTGGTTACCTTTAACGATGTAATGGCCGCAGGTTTAATGGCAAGCCTGCATGAAAATAATATTCGTGTTCCAGACGATAT
TTCTATTATTGGGTTTGACGATGTTTTATTGGCTCGATATCTCTACCCACGCTTAACCACAATGCACAATCCTATTGATG
AAATGTCGAGCTATGCTGCTGATTTAGCATTAAAAATAAAAAACACCGGTTATGTTCCACCATCTAATCATCGCTTTAAT
GCCAGCTTGGTCGAGCGACAAACTGTTATACCTTTTTCAAATAAACAAGCCCTAAAAGTGTGA

Upstream 100 bases:

>100_bases
TTATAAACGGCATGCTCCACAGAATTTGATAGTTCTAACATCTTTATCGAAAAATCCGCGCTACACTCTGACTCAATGTT
AATCACCATAAGTAGTCATC

Downstream 100 bases:

>100_bases
TGAGCATCTTAGGTAAGAACTTCATGTAAGCGCTTACATGAGTGCGTTACCTGTCTCTCATATTCTAATTAATAGAACTC
TTATTATGACCTCATAAAAA

Product: transcriptional repressor

Products: NA

Alternate protein names: Galactose operon repressor [H]

Number of amino acids: Translated: 340; Mature: 339

Protein sequence:

>340_residues
MATISDVAKLARVSTATVSRVINNSAYVEPVTLERVQSAIRELNYQRDARASALAKKTNNTLGLLTGNLADPFFALIAKH
VEEVARKHKCQLVVVSGGHDAQREKEGLDFLISQGCEAMVVHAKMLDDATLLRYSAQLPSMVLLNRTIPQISNRSVYIDN
RAGAKESVVYLIKQGHRKIACVTSDLPIEDRTERLNGYRDAMAEANITIEPNWIINQNFSEQGGELAGEQLLFQCPEVTA
VVTFNDVMAAGLMASLHENNIRVPDDISIIGFDDVLLARYLYPRLTTMHNPIDEMSSYAADLALKIKNTGYVPPSNHRFN
ASLVERQTVIPFSNKQALKV

Sequences:

>Translated_340_residues
MATISDVAKLARVSTATVSRVINNSAYVEPVTLERVQSAIRELNYQRDARASALAKKTNNTLGLLTGNLADPFFALIAKH
VEEVARKHKCQLVVVSGGHDAQREKEGLDFLISQGCEAMVVHAKMLDDATLLRYSAQLPSMVLLNRTIPQISNRSVYIDN
RAGAKESVVYLIKQGHRKIACVTSDLPIEDRTERLNGYRDAMAEANITIEPNWIINQNFSEQGGELAGEQLLFQCPEVTA
VVTFNDVMAAGLMASLHENNIRVPDDISIIGFDDVLLARYLYPRLTTMHNPIDEMSSYAADLALKIKNTGYVPPSNHRFN
ASLVERQTVIPFSNKQALKV
>Mature_339_residues
ATISDVAKLARVSTATVSRVINNSAYVEPVTLERVQSAIRELNYQRDARASALAKKTNNTLGLLTGNLADPFFALIAKHV
EEVARKHKCQLVVVSGGHDAQREKEGLDFLISQGCEAMVVHAKMLDDATLLRYSAQLPSMVLLNRTIPQISNRSVYIDNR
AGAKESVVYLIKQGHRKIACVTSDLPIEDRTERLNGYRDAMAEANITIEPNWIINQNFSEQGGELAGEQLLFQCPEVTAV
VTFNDVMAAGLMASLHENNIRVPDDISIIGFDDVLLARYLYPRLTTMHNPIDEMSSYAADLALKIKNTGYVPPSNHRFNA
SLVERQTVIPFSNKQALKV

Specific function: Repressor of the galactose operon. Binds galactose as an inducer [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789202, Length=329, Percent_Identity=44.6808510638298, Blast_Score=262, Evalue=2e-71,
Organism=Escherichia coli, GI1788474, Length=338, Percent_Identity=39.0532544378698, Blast_Score=244, Evalue=9e-66,
Organism=Escherichia coli, GI1789068, Length=335, Percent_Identity=32.8358208955224, Blast_Score=179, Evalue=2e-46,
Organism=Escherichia coli, GI1790369, Length=325, Percent_Identity=31.6923076923077, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI1790194, Length=313, Percent_Identity=34.8242811501597, Blast_Score=170, Evalue=1e-43,
Organism=Escherichia coli, GI1787948, Length=339, Percent_Identity=32.1533923303835, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1787580, Length=339, Percent_Identity=25.6637168141593, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1787906, Length=263, Percent_Identity=27.7566539923954, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI48994940, Length=313, Percent_Identity=25.8785942492013, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1786540, Length=335, Percent_Identity=27.7611940298507, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1790715, Length=316, Percent_Identity=28.4810126582279, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1786268, Length=287, Percent_Identity=24.0418118466899, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789456, Length=330, Percent_Identity=24.2424242424242, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37637; Mature: 37505

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATISDVAKLARVSTATVSRVINNSAYVEPVTLERVQSAIRELNYQRDARASALAKKTNN
CCCHHHHHHHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
TLGLLTGNLADPFFALIAKHVEEVARKHKCQLVVVSGGHDAQREKEGLDFLISQGCEAMV
EEEEEECCCCHHHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHCCCCEEE
VHAKMLDDATLLRYSAQLPSMVLLNRTIPQISNRSVYIDNRAGAKESVVYLIKQGHRKIA
HHHHHHCCHHHHHHHHCCCHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCEEE
CVTSDLPIEDRTERLNGYRDAMAEANITIEPNWIINQNFSEQGGELAGEQLLFQCPEVTA
EEECCCCCCHHHHHHHHHHHHHHHCCEEECCCEEECCCCHHCCCHHHHHHHHHCCCCEEE
VVTFNDVMAAGLMASLHENNIRVPDDISIIGFDDVLLARYLYPRLTTMHNPIDEMSSYAA
EEEEHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
DLALKIKNTGYVPPSNHRFNASLVERQTVIPFSNKQALKV
HEEEEECCCCCCCCCCCCCCHHHHCCEEECCCCCCCCCCC
>Mature Secondary Structure 
ATISDVAKLARVSTATVSRVINNSAYVEPVTLERVQSAIRELNYQRDARASALAKKTNN
CCHHHHHHHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
TLGLLTGNLADPFFALIAKHVEEVARKHKCQLVVVSGGHDAQREKEGLDFLISQGCEAMV
EEEEEECCCCHHHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHCCCCEEE
VHAKMLDDATLLRYSAQLPSMVLLNRTIPQISNRSVYIDNRAGAKESVVYLIKQGHRKIA
HHHHHHCCHHHHHHHHCCCHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCEEE
CVTSDLPIEDRTERLNGYRDAMAEANITIEPNWIINQNFSEQGGELAGEQLLFQCPEVTA
EEECCCCCCHHHHHHHHHHHHHHHCCEEECCCEEECCCCHHCCCHHHHHHHHHCCCCEEE
VVTFNDVMAAGLMASLHENNIRVPDDISIIGFDDVLLARYLYPRLTTMHNPIDEMSSYAA
EEEEHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
DLALKIKNTGYVPPSNHRFNASLVERQTVIPFSNKQALKV
HEEEEECCCCCCCCCCCCCCHHHHCCEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6283521; 9278503; 6350601; 8188660; 8982002 [H]