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The map label for this gene is ycdT [H]

Identifier: 59711852

GI number: 59711852

Start: 1385030

End: 1386580

Strand: Reverse

Name: ycdT [H]

Synonym: VF_1245

Alternate gene names: 59711852

Gene position: 1386580-1385030 (Counterclockwise)

Preceding gene: 59711853

Following gene: 59711850

Centisome position: 47.85

GC content: 35.33

Gene sequence:

>1551_bases
ATGCCTCAATCTCGTCTTCAGCGCTTATCGTTCTTACAACCAATTATCATCTTACTCCTGGTTGGGATGGCTGCTATTTC
ACTCACGTTTAGTGTCAAAAACTTTAAATCTGTTGAAAAAACAATCAAAACATCATCCTTTCAGGCATTCAACAACTCAA
TTGAAGCCAGTTACGATATTGTAGACACTGCACTTAATGAATCTATCAAACGTTATTTAAAAGGCATAGTGACAACCTCT
GCTGAATTTATTTCTCAAATTCAAAACTCTCCAGTATTAAGTGCTGAGCAAAAAAAAAGAGTCATACAAGACTATATTAA
CGACAGCAAAATTGGAGACACTGGCTATCCTTACATTCTAAACTCGAATGGGGATTTACTGTACCACCCTATCTTTCAGG
GTCGTAATGTTGGCCAGTACCTGCATATTCAGCAACAAATTGGCAATGATGATAATTTTGTTGAGTATTTATGGACAAAC
CCTGGAGATGAGAAGCCAAGAAAGAAAATCACTTACTCGGTTTACTTGCCTGATTCTGATCTTATCGTATCAGCAAGTGC
TTATAAGGACGAACTTCTTCACTTTATTGATAAAAGCTTACTGAAAGGCAAGTTAAATAAATATCAATATGGTGAAACTG
GTTATGTCTATGTCATCGACCTAGATGGTAAGTTATTGCTTCACCCTAGCTATGAAGGCGAAAATGTTCGTGACTTAATT
GGTTCTCACGCTGATGGACTGTTAGAAAAAATACGTCAGTCTGCTCATGCGTACAATTTACAGATGAATCAACACCAAAC
CACTTCAAGTGATCATTCTCGTATGGATATTGAGGATTACACTTACCAAATTACTTTAGATAATAAAACGTATCAAAAAG
ATGTGTTGTTTATCTATTACCCTTATTTGGACTGGGCGATCATTTCAGGTATTTCTCGAGATGAATTAAATCAACCAACC
AATACATTGCTATATAGCTTGCTGACTTTAGTGATCAGCTTAACGACTTTAATCTTGATTTTGATGTTGTTACTTAACCA
TCGCCATAGAAAATTAATTAATGTACTTAATAGAGACTATTTAACTGGCTTATACAACCGTGGGACGTTTCATAACAAGG
TATCGCCACTCATAAAATCGAGTAACAGTAATAACAAATTTAAGCCCTATTCGATTATTTTATTGGATATCGATTTCTTT
AAACGTATTAATGATAATTACGGACATTTGGTTGGTGATAAGGTGATCGCTATCGTGGGTAGAACAATGGCTCAATACCC
TAACGTATTAGCTGGTCGTTATGGTGGTGAGGAGTTTATTCTTTTTATTCGAGATAATGACATTGAGTCAGTGATTGAAT
TTACTGACCAAATACGTACGCAAATCTCAAACTATAATACGCTTCATGAAGACATTACTCTGAGCGCTGGGATTACGACA
ATTAATAAACCAAACGTACTATTAGATGATGTAATTGAACAAGCAGATAAGGCGCTATATTACTCAAAACATAATGGGCG
TAATAAAATCACTCACTATCAAGATATATAA

Upstream 100 bases:

>100_bases
TTTGTCTATTTCTCATTCAATCCGAATAAAATCAGAACATATTGATAGAGTCCATACTTTCCAATACAATTGCATATACC
GTTAAAAGGATTTTAATCTT

Downstream 100 bases:

>100_bases
TTGCAGAAAATAAACTGAAGAAAAACTGGACAGGACGAGCCCTTGAACTACGCTAAATAAGGTAATGCTTTCCATTTTAT
TATTAGCGCAAGGCTCGCGA

Product: GGDEF domain-containing protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 516; Mature: 515

Protein sequence:

>516_residues
MPQSRLQRLSFLQPIIILLLVGMAAISLTFSVKNFKSVEKTIKTSSFQAFNNSIEASYDIVDTALNESIKRYLKGIVTTS
AEFISQIQNSPVLSAEQKKRVIQDYINDSKIGDTGYPYILNSNGDLLYHPIFQGRNVGQYLHIQQQIGNDDNFVEYLWTN
PGDEKPRKKITYSVYLPDSDLIVSASAYKDELLHFIDKSLLKGKLNKYQYGETGYVYVIDLDGKLLLHPSYEGENVRDLI
GSHADGLLEKIRQSAHAYNLQMNQHQTTSSDHSRMDIEDYTYQITLDNKTYQKDVLFIYYPYLDWAIISGISRDELNQPT
NTLLYSLLTLVISLTTLILILMLLLNHRHRKLINVLNRDYLTGLYNRGTFHNKVSPLIKSSNSNNKFKPYSIILLDIDFF
KRINDNYGHLVGDKVIAIVGRTMAQYPNVLAGRYGGEEFILFIRDNDIESVIEFTDQIRTQISNYNTLHEDITLSAGITT
INKPNVLLDDVIEQADKALYYSKHNGRNKITHYQDI

Sequences:

>Translated_516_residues
MPQSRLQRLSFLQPIIILLLVGMAAISLTFSVKNFKSVEKTIKTSSFQAFNNSIEASYDIVDTALNESIKRYLKGIVTTS
AEFISQIQNSPVLSAEQKKRVIQDYINDSKIGDTGYPYILNSNGDLLYHPIFQGRNVGQYLHIQQQIGNDDNFVEYLWTN
PGDEKPRKKITYSVYLPDSDLIVSASAYKDELLHFIDKSLLKGKLNKYQYGETGYVYVIDLDGKLLLHPSYEGENVRDLI
GSHADGLLEKIRQSAHAYNLQMNQHQTTSSDHSRMDIEDYTYQITLDNKTYQKDVLFIYYPYLDWAIISGISRDELNQPT
NTLLYSLLTLVISLTTLILILMLLLNHRHRKLINVLNRDYLTGLYNRGTFHNKVSPLIKSSNSNNKFKPYSIILLDIDFF
KRINDNYGHLVGDKVIAIVGRTMAQYPNVLAGRYGGEEFILFIRDNDIESVIEFTDQIRTQISNYNTLHEDITLSAGITT
INKPNVLLDDVIEQADKALYYSKHNGRNKITHYQDI
>Mature_515_residues
PQSRLQRLSFLQPIIILLLVGMAAISLTFSVKNFKSVEKTIKTSSFQAFNNSIEASYDIVDTALNESIKRYLKGIVTTSA
EFISQIQNSPVLSAEQKKRVIQDYINDSKIGDTGYPYILNSNGDLLYHPIFQGRNVGQYLHIQQQIGNDDNFVEYLWTNP
GDEKPRKKITYSVYLPDSDLIVSASAYKDELLHFIDKSLLKGKLNKYQYGETGYVYVIDLDGKLLLHPSYEGENVRDLIG
SHADGLLEKIRQSAHAYNLQMNQHQTTSSDHSRMDIEDYTYQITLDNKTYQKDVLFIYYPYLDWAIISGISRDELNQPTN
TLLYSLLTLVISLTTLILILMLLLNHRHRKLINVLNRDYLTGLYNRGTFHNKVSPLIKSSNSNNKFKPYSIILLDIDFFK
RINDNYGHLVGDKVIAIVGRTMAQYPNVLAGRYGGEEFILFIRDNDIESVIEFTDQIRTQISNYNTLHEDITLSAGITTI
NKPNVLLDDVIEQADKALYYSKHNGRNKITHYQDI

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=191, Percent_Identity=31.413612565445, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788381, Length=172, Percent_Identity=33.7209302325581, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87082007, Length=202, Percent_Identity=30.6930693069307, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI87081881, Length=163, Percent_Identity=30.6748466257669, Blast_Score=86, Evalue=8e-18,
Organism=Escherichia coli, GI1787816, Length=160, Percent_Identity=33.75, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI145693134, Length=173, Percent_Identity=30.0578034682081, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1786584, Length=168, Percent_Identity=29.1666666666667, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788956, Length=176, Percent_Identity=31.25, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1787802, Length=164, Percent_Identity=32.3170731707317, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1788085, Length=163, Percent_Identity=35.5828220858896, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI87081974, Length=183, Percent_Identity=28.4153005464481, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1787541, Length=174, Percent_Identity=27.0114942528736, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 59147; Mature: 59016

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQSRLQRLSFLQPIIILLLVGMAAISLTFSVKNFKSVEKTIKTSSFQAFNNSIEASYDI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
VDTALNESIKRYLKGIVTTSAEFISQIQNSPVLSAEQKKRVIQDYINDSKIGDTGYPYIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEE
NSNGDLLYHPIFQGRNVGQYLHIQQQIGNDDNFVEYLWTNPGDEKPRKKITYSVYLPDSD
CCCCCEEEECCCCCCCCCHHEEEHHHCCCCCCEEEEEECCCCCCCCCCEEEEEEEECCCC
LIVSASAYKDELLHFIDKSLLKGKLNKYQYGETGYVYVIDLDGKLLLHPSYEGENVRDLI
EEEECHHHHHHHHHHHHHHHHHHHCCCEECCCCCEEEEEEECCEEEECCCCCCCHHHHHH
GSHADGLLEKIRQSAHAYNLQMNQHQTTSSDHSRMDIEDYTYQITLDNKTYQKDVLFIYY
CCCHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEEEE
PYLDWAIISGISRDELNQPTNTLLYSLLTLVISLTTLILILMLLLNHRHRKLINVLNRDY
CHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LTGLYNRGTFHNKVSPLIKSSNSNNKFKPYSIILLDIDFFKRINDNYGHLVGDKVIAIVG
HHHHHCCCCCHHHHHHHHHCCCCCCCCCCEEEEEEEHHHHHHCCCCCCHHHHHHHHHHHH
RTMAQYPNVLAGRYGGEEFILFIRDNDIESVIEFTDQIRTQISNYNTLHEDITLSAGITT
HHHHHCCCHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCHHHHCEEEECCEEE
INKPNVLLDDVIEQADKALYYSKHNGRNKITHYQDI
CCCCCHHHHHHHHHHHHHEEEECCCCCCCCCCCCCC
>Mature Secondary Structure 
PQSRLQRLSFLQPIIILLLVGMAAISLTFSVKNFKSVEKTIKTSSFQAFNNSIEASYDI
CHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
VDTALNESIKRYLKGIVTTSAEFISQIQNSPVLSAEQKKRVIQDYINDSKIGDTGYPYIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEE
NSNGDLLYHPIFQGRNVGQYLHIQQQIGNDDNFVEYLWTNPGDEKPRKKITYSVYLPDSD
CCCCCEEEECCCCCCCCCHHEEEHHHCCCCCCEEEEEECCCCCCCCCCEEEEEEEECCCC
LIVSASAYKDELLHFIDKSLLKGKLNKYQYGETGYVYVIDLDGKLLLHPSYEGENVRDLI
EEEECHHHHHHHHHHHHHHHHHHHCCCEECCCCCEEEEEEECCEEEECCCCCCCHHHHHH
GSHADGLLEKIRQSAHAYNLQMNQHQTTSSDHSRMDIEDYTYQITLDNKTYQKDVLFIYY
CCCHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEEEE
PYLDWAIISGISRDELNQPTNTLLYSLLTLVISLTTLILILMLLLNHRHRKLINVLNRDY
CHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LTGLYNRGTFHNKVSPLIKSSNSNNKFKPYSIILLDIDFFKRINDNYGHLVGDKVIAIVG
HHHHHCCCCCHHHHHHHHHCCCCCCCCCCEEEEEEEHHHHHHCCCCCCHHHHHHHHHHHH
RTMAQYPNVLAGRYGGEEFILFIRDNDIESVIEFTDQIRTQISNYNTLHEDITLSAGITT
HHHHHCCCHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCHHHHCEEEECCEEE
INKPNVLLDDVIEQADKALYYSKHNGRNKITHYQDI
CCCCCHHHHHHHHHHHHHEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]