Click here to switch to the map view.

The map label for this gene is ppsA [H]

Identifier: 59711849

GI number: 59711849

Start: 1381620

End: 1382504

Strand: Direct

Name: ppsA [H]

Synonym: VF_1242

Alternate gene names: 59711849

Gene position: 1381620-1382504 (Clockwise)

Preceding gene: 59711846

Following gene: 59711851

Centisome position: 47.68

GC content: 39.21

Gene sequence:

>885_bases
ATGACAGACACAATTTTACCGACACTTAATCGTGTTGCGAGTTTACCTTCATATCAATCAGAGACAGACTCAGACGCTTC
ACTATATGTATCTATTGCTGCATTAATTCAAGAGCATTTGTTTTATCACCCAAAAGCAGTAATCAAAGCACCTGAAATGA
CGTCAATTGATGCTCAAAGTTTTGCTGCAATCATTGAAAGTGCGGCTGATGGAAAAACGCATTTTATTGATACACTAGCA
AACGTGATTTTATCGAATGTTACTGAAGCGACAAAAGAAATCAAAATTGCATTTTCAGATGCGGATAGCGCGGCTTTTGC
AGGCCTTCTTGCGGGTCAACTAGAAGAGAAAGAAACCAATCCAATGATGGGAGTTCGAGGTGTAACTCGATACGCTTCTG
ACGCTAGCGCCGATCAATTTGCACTAGAGTGTGAAGTGATCAAAAAGGTCATTGCAAACACATCAGCAAGTGTGCAAATC
GTCGTACCATTTGTTCGTAATTTAAGTGATGCTGCAACCATTATTGATCGCTTAGCGATTCAAGGCTTACCGCGTGGTTT
AGATAGTTTAAAAGTACACTTTGTGTGTGATGTGCCAGCTGCAGCATTAATGGCAGATAAATTACTGCAATACTTTGATG
GTATGGTGATTAATGTTGATACATTAACGCAGTTTACATTAGGCGTTGATAAGCAAAATGAAGCATTAGAACATCATTTT
GAACCACAAAACGATGCGGTTATACACCTTATTGGACATGCTATTCAATCAGCTAATAAAGCAAATAAGCCAGTGTTAAC
CACCATTGATGAACTGGCTCAAAACCTTCGTATTCAAGAGTTGCTAGAGCAACTGAATGTCGTAGATGTTGTAGTTACAG
CGTAA

Upstream 100 bases:

>100_bases
TTAACTTGTTGTATCTTTTGGACTTGAGTTGTTGGCAATGTATCGGTATGTTCACATTAATAACAACATAAATGGTATTT
ACGCGTTTACTGGAGCAAGC

Downstream 100 bases:

>100_bases
CTTAATATTATTAAGTAGCAATAAAAAACGCCTTTATTTCGTTAAGCAATAAAGGCGTTTTTTTGTATCATAAGCTGTTT
AATTATTTTTCGTCAGATAT

Product: phosphoenolpyruvate synthase

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MTDTILPTLNRVASLPSYQSETDSDASLYVSIAALIQEHLFYHPKAVIKAPEMTSIDAQSFAAIIESAADGKTHFIDTLA
NVILSNVTEATKEIKIAFSDADSAAFAGLLAGQLEEKETNPMMGVRGVTRYASDASADQFALECEVIKKVIANTSASVQI
VVPFVRNLSDAATIIDRLAIQGLPRGLDSLKVHFVCDVPAAALMADKLLQYFDGMVINVDTLTQFTLGVDKQNEALEHHF
EPQNDAVIHLIGHAIQSANKANKPVLTTIDELAQNLRIQELLEQLNVVDVVVTA

Sequences:

>Translated_294_residues
MTDTILPTLNRVASLPSYQSETDSDASLYVSIAALIQEHLFYHPKAVIKAPEMTSIDAQSFAAIIESAADGKTHFIDTLA
NVILSNVTEATKEIKIAFSDADSAAFAGLLAGQLEEKETNPMMGVRGVTRYASDASADQFALECEVIKKVIANTSASVQI
VVPFVRNLSDAATIIDRLAIQGLPRGLDSLKVHFVCDVPAAALMADKLLQYFDGMVINVDTLTQFTLGVDKQNEALEHHF
EPQNDAVIHLIGHAIQSANKANKPVLTTIDELAQNLRIQELLEQLNVVDVVVTA
>Mature_293_residues
TDTILPTLNRVASLPSYQSETDSDASLYVSIAALIQEHLFYHPKAVIKAPEMTSIDAQSFAAIIESAADGKTHFIDTLAN
VILSNVTEATKEIKIAFSDADSAAFAGLLAGQLEEKETNPMMGVRGVTRYASDASADQFALECEVIKKVIANTSASVQIV
VPFVRNLSDAATIIDRLAIQGLPRGLDSLKVHFVCDVPAAALMADKLLQYFDGMVINVDTLTQFTLGVDKQNEALEHHFE
PQNDAVIHLIGHAIQSANKANKPVLTTIDELAQNLRIQELLEQLNVVDVVVTA

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=176, Percent_Identity=40.9090909090909, Blast_Score=147, Evalue=8e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR006319
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.2 [H]

Molecular weight: Translated: 31785; Mature: 31653

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTILPTLNRVASLPSYQSETDSDASLYVSIAALIQEHLFYHPKAVIKAPEMTSIDAQS
CCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHEECCCCCCCCHHH
FAAIIESAADGKTHFIDTLANVILSNVTEATKEIKIAFSDADSAAFAGLLAGQLEEKETN
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHCCC
PMMGVRGVTRYASDASADQFALECEVIKKVIANTSASVQIVVPFVRNLSDAATIIDRLAI
CCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEHHHHCCHHHHHHHHHHHH
QGLPRGLDSLKVHFVCDVPAAALMADKLLQYFDGMVINVDTLTQFTLGVDKQNEALEHHF
HCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCEEEEEHHHHHHHHCCCCCHHHHHHCC
EPQNDAVIHLIGHAIQSANKANKPVLTTIDELAQNLRIQELLEQLNVVDVVVTA
CCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHCCHHEEEEEC
>Mature Secondary Structure 
TDTILPTLNRVASLPSYQSETDSDASLYVSIAALIQEHLFYHPKAVIKAPEMTSIDAQS
CCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHEECCCCCCCCHHH
FAAIIESAADGKTHFIDTLANVILSNVTEATKEIKIAFSDADSAAFAGLLAGQLEEKETN
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHCCC
PMMGVRGVTRYASDASADQFALECEVIKKVIANTSASVQIVVPFVRNLSDAATIIDRLAI
CCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEHHHHCCHHHHHHHHHHHH
QGLPRGLDSLKVHFVCDVPAAALMADKLLQYFDGMVINVDTLTQFTLGVDKQNEALEHHF
HCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCEEEEEHHHHHHHHCCCCCHHHHHHCC
EPQNDAVIHLIGHAIQSANKANKPVLTTIDELAQNLRIQELLEQLNVVDVVVTA
CCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHCCHHEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1310524; 9097039; 9278503 [H]