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The map label for this gene is nrdB [H]

Identifier: 59711808

GI number: 59711808

Start: 1334893

End: 1336026

Strand: Reverse

Name: nrdB [H]

Synonym: VF_1201

Alternate gene names: 59711808

Gene position: 1336026-1334893 (Counterclockwise)

Preceding gene: 59711809

Following gene: 59711807

Centisome position: 46.11

GC content: 36.86

Gene sequence:

>1134_bases
ATGGCGTACAGTACTTTCCGACAAGAAAAAAATGATCAATTGAAAGAACCTATGTTTTTAGGTAACTCGGTAAACGTTGC
TCGTTATGACCAACAAAAGTTTCAAATTTTTGAAAAACTTATCGAAAAACAACTTTCATTTTTCTGGCGTCCGGAAGAAG
TTGATGTTTCTGGTGACCGTATTGATTACAACAACTTACCAGAACACGAACAACACATCTTCATCAGCAACTTAAAATAC
CAAACGTTACTTGATTCAATTCAAGGTCGTAGTCCAAATGTTGCTTTACTTCCTCTTGTTTCTATTCCTGAACTAGAGAC
GTGGATTGAAACTTGGTCGTTCTCTGAAACAATTCACTCACGTTCTTATACTCATATTATTCGTAATATCGTTAATAACC
CTTCTGTTATTTTTGACGATATTGTTGAAAATGAAGAGATCCTAAAACGTGCAAAAGACATTGCTCACTATTACGATAAT
CTAATTCAAGCAACAAATGATTATCATCGCTTCGGTGAAGGTACACACGTTTTAAACGGTCAAGAAATTACTATTTCTAA
GCGTGAGCTTAAAAAGAAATTATACCTATGCTTAATGTCAGTTAATGCACTAGAAGCCATTCGATTCTACGTAAGTTTTG
CTTGTTCATTTGCTTTTGCTGAACGTGAACTGATGGAAGGTAACGCAAAAATCATCAAGCTGATCGCTCGTGATGAATCA
TTACACCTAACAAGTACTCAACACATGTTGAACTTACTTCGTACTGGACAAGATGACCCTGAGTTTGTTGAGATTGCAGC
AGAATGCGAACAAGAATGTTTTGATCTATTTAAAGCAGCAGCTGAGCAAGAAAAACAATGGGCAAACTACCTATTTAAAG
ATGGCTCAATGATTGGTTTAAACAAAGATATTCTTTGCCAATACGTAGAGTATATTACAAACCTTCGCATGAAAGCGGTT
GGTCTAAATAGTGCGTACGAAGGCGCAACACAGAACCCTATCCCATGGATTAACTCATGGTTAAGTTCTGACAACGTTCA
AGTTGCTCCACAAGAAACCGAAATCAGCTCCTACCTAGTAGGCCAAATTGACAATGATGTTGATGCAGACGACTTAGGCG
ATTTCGAATTATAA

Upstream 100 bases:

>100_bases
AATCCAATATGTACCTAAGTTCGTATAGGTATCTATCGGCATAATTTTTAAATAAAGAGCCCTTTTCTATTTCTTTGGCT
TTGATAACATTGAGGTTTCC

Downstream 100 bases:

>100_bases
ACAAACATATATAAACGCCAAAGCCGAGCTTTCATGCTCGGCTTTTTATTATCAGTAATAAACCTTATTGATAGATTTGC
GGATAATAATTCAACCTTGC

Product: ribonucleotide-diphosphate reductase subunit beta

Products: NA

Alternate protein names: Protein B2; Protein R2; Ribonucleotide reductase 1 [H]

Number of amino acids: Translated: 377; Mature: 376

Protein sequence:

>377_residues
MAYSTFRQEKNDQLKEPMFLGNSVNVARYDQQKFQIFEKLIEKQLSFFWRPEEVDVSGDRIDYNNLPEHEQHIFISNLKY
QTLLDSIQGRSPNVALLPLVSIPELETWIETWSFSETIHSRSYTHIIRNIVNNPSVIFDDIVENEEILKRAKDIAHYYDN
LIQATNDYHRFGEGTHVLNGQEITISKRELKKKLYLCLMSVNALEAIRFYVSFACSFAFAERELMEGNAKIIKLIARDES
LHLTSTQHMLNLLRTGQDDPEFVEIAAECEQECFDLFKAAAEQEKQWANYLFKDGSMIGLNKDILCQYVEYITNLRMKAV
GLNSAYEGATQNPIPWINSWLSSDNVQVAPQETEISSYLVGQIDNDVDADDLGDFEL

Sequences:

>Translated_377_residues
MAYSTFRQEKNDQLKEPMFLGNSVNVARYDQQKFQIFEKLIEKQLSFFWRPEEVDVSGDRIDYNNLPEHEQHIFISNLKY
QTLLDSIQGRSPNVALLPLVSIPELETWIETWSFSETIHSRSYTHIIRNIVNNPSVIFDDIVENEEILKRAKDIAHYYDN
LIQATNDYHRFGEGTHVLNGQEITISKRELKKKLYLCLMSVNALEAIRFYVSFACSFAFAERELMEGNAKIIKLIARDES
LHLTSTQHMLNLLRTGQDDPEFVEIAAECEQECFDLFKAAAEQEKQWANYLFKDGSMIGLNKDILCQYVEYITNLRMKAV
GLNSAYEGATQNPIPWINSWLSSDNVQVAPQETEISSYLVGQIDNDVDADDLGDFEL
>Mature_376_residues
AYSTFRQEKNDQLKEPMFLGNSVNVARYDQQKFQIFEKLIEKQLSFFWRPEEVDVSGDRIDYNNLPEHEQHIFISNLKYQ
TLLDSIQGRSPNVALLPLVSIPELETWIETWSFSETIHSRSYTHIIRNIVNNPSVIFDDIVENEEILKRAKDIAHYYDNL
IQATNDYHRFGEGTHVLNGQEITISKRELKKKLYLCLMSVNALEAIRFYVSFACSFAFAERELMEGNAKIIKLIARDESL
HLTSTQHMLNLLRTGQDDPEFVEIAAECEQECFDLFKAAAEQEKQWANYLFKDGSMIGLNKDILCQYVEYITNLRMKAVG
LNSAYEGATQNPIPWINSWLSSDNVQVAPQETEISSYLVGQIDNDVDADDLGDFEL

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. R2 contains the tyrosyl radical required for catalysis [H]

COG id: COG0208

COG function: function code F; Ribonucleotide reductase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase small chain family [H]

Homologues:

Organism=Escherichia coli, GI1788567, Length=377, Percent_Identity=79.8408488063661, Blast_Score=644, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009078
- InterPro:   IPR012348
- InterPro:   IPR000358 [H]

Pfam domain/function: PF00268 Ribonuc_red_sm [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 43583; Mature: 43451

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: PS00368 RIBORED_SMALL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYSTFRQEKNDQLKEPMFLGNSVNVARYDQQKFQIFEKLIEKQLSFFWRPEEVDVSGDR
CCCCHHHHHHHHHHHCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
IDYNNLPEHEQHIFISNLKYQTLLDSIQGRSPNVALLPLVSIPELETWIETWSFSETIHS
CCCCCCCCHHHEEEECCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHH
RSYTHIIRNIVNNPSVIFDDIVENEEILKRAKDIAHYYDNLIQATNDYHRFGEGTHVLNG
HHHHHHHHHHHCCCCEEHHHHHCCHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCEEECC
QEITISKRELKKKLYLCLMSVNALEAIRFYVSFACSFAFAERELMEGNAKIIKLIARDES
CEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEEEECCCC
LHLTSTQHMLNLLRTGQDDPEFVEIAAECEQECFDLFKAAAEQEKQWANYLFKDGSMIGL
EEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECC
NKDILCQYVEYITNLRMKAVGLNSAYEGATQNPIPWINSWLSSDNVQVAPQETEISSYLV
CHHHHHHHHHHHHCCHHHEECCCCCCCCCCCCCCHHHHHHHCCCCCEECCCHHHHHHHHH
GQIDNDVDADDLGDFEL
HCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AYSTFRQEKNDQLKEPMFLGNSVNVARYDQQKFQIFEKLIEKQLSFFWRPEEVDVSGDR
CCCHHHHHHHHHHHCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
IDYNNLPEHEQHIFISNLKYQTLLDSIQGRSPNVALLPLVSIPELETWIETWSFSETIHS
CCCCCCCCHHHEEEECCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHH
RSYTHIIRNIVNNPSVIFDDIVENEEILKRAKDIAHYYDNLIQATNDYHRFGEGTHVLNG
HHHHHHHHHHHCCCCEEHHHHHCCHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCEEECC
QEITISKRELKKKLYLCLMSVNALEAIRFYVSFACSFAFAERELMEGNAKIIKLIARDES
CEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEEEECCCC
LHLTSTQHMLNLLRTGQDDPEFVEIAAECEQECFDLFKAAAEQEKQWANYLFKDGSMIGL
EEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECC
NKDILCQYVEYITNLRMKAVGLNSAYEGATQNPIPWINSWLSSDNVQVAPQETEISSYLV
CHHHHHHHHHHHHCCHHHEECCCCCCCCCCCCCCHHHHHHHCCCCCEECCCHHHHHHHHH
GQIDNDVDADDLGDFEL
HCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]