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The map label for this gene is b2657; [C]

Identifier: 59711739

GI number: 59711739

Start: 1253587

End: 1254969

Strand: Direct

Name: b2657; [C]

Synonym: VF_1132

Alternate gene names: 59711739

Gene position: 1253587-1254969 (Clockwise)

Preceding gene: 59711738

Following gene: 59711741

Centisome position: 43.26

GC content: 40.56

Gene sequence:

>1383_bases
ATGAGTCAAGCAACACAGCAGCACTCCGATCCGGTTCCTATCGTGAGTAACGTGATACTTCACGCTTTTGATTGGAGCTA
TCAACGAATTACTGAACAAGCTGAATTAATTGCTCAATTGGGTTATCGCTCAGTGCTTGTTTCTCCTGCCATGAAATCTC
TAAATTTGCCATCTGGAACTAAGTGGTGGCAACGTTATCAACCTCAAGATTACCGTTTGATTGATAACCCTCTTGGCGAT
ACGTTTGATTTTAAACGCATGGTTGAATGTTTAACTGAGCTTAATATTTGGGTTTATGTGGATGTGGTGTTTAATCACAT
GGCCAACGAATCGGATATTCGAGCTGATTTAGAATACCCAAATCAATGGGACAGAGAAGATTACGCTAAAGATCCTGAAA
AGTACGAGGCATTAACTTTATTTGGTGATTTATCTGAGCCTCTGTTTACTGAGAACGATTTTGTTGAAGCGTTTGGCATT
GAGAATTGGAATGATAAATGGGAAGTGCAGAACGGGCGTATTTCAGGTGGTCCACACGATCCCGGTTTACCGACATTAGC
CGATAATGAACACGTTATTGCACAGCAACGTGCGTATTTAAAAGCGTTAAAAGCCATTGGAGTGAAAGGCTTTCGGATTG
ATGCCGCAAAGCACATGAGTCTTGAGCATCTTGAGAAAGTATGGGATGAAGAGATCACTCATGATATTCATATCTTTGGT
GAGATCATTACCGATGGTGGTGCAACAAAAGAAGAGTACGAAACCTTTTTAAGCCCATATTTACAAGAAACTAAATTAGG
TGCTTATGATTTCCCTCTGTTTAATACCTTGTATCAAGCGCTAGAGGGAGAAGCGTCATTAACTAGTCTGATAGACCCGT
ATATCTATGGCATGGCACTTTCTCCATTACGTGCAATTACCTTTGCTACCACTCATGACATTCCTAATAACCAGGTGTTT
CAAGACCTTGTAATGTCTGAAGAAAACGAATGGCTAGCTTATGCGTATTTGTTTGGGCGTGATGGTGGCATTCCGCTTAT
TTACTCAGAGAATGAGATCAGTGGATTTAAAAATACGGAAGGTAATCCACGCTGGAAAGATGAGTGGTGCTCTAAACGCA
TGACGCAATTGATTGGGTTTTATCATGAAATGTATGGCGAAACTCAAACGCAAGTTGAAGCCAGTGATGAGCATTTGGTA
TTTGCACGAGGAGATCGTGGTTTAGTCGCTATCAATAAATCTGATCATAATATTGAAGTGAATGTCGCGGTTAAGGCAGA
TATTGTTTGGTTAGAGCATACTTCTCAAACTTATCATGCACCGCAGCAAGGGAATTTGCGTTTCTTTATTCCTGCAAAAT
CGTATTTGCTGTTTTCTCGTTAA

Upstream 100 bases:

>100_bases
CGCCTTTCGACTCCTACTGTATTTTATAGAATGGGGAGGAGGGCAACGAAAAACGGCCTTTTTATAATTGGCTCACATCA
TAAAAAAGGATAATAATGAA

Downstream 100 bases:

>100_bases
TTGTTCGTTTTAGAGTAAAGCGTAATAAAAAAGGTGGATAATTTATCCACCTTTTTTGTTTGTGACATTGCTTATCTAAG
ATAATTACACTTTAAATTGA

Product: alpha-amylase

Products: NA

Alternate protein names: 1,4-alpha-D-glucan glucanohydrolase [H]

Number of amino acids: Translated: 460; Mature: 459

Protein sequence:

>460_residues
MSQATQQHSDPVPIVSNVILHAFDWSYQRITEQAELIAQLGYRSVLVSPAMKSLNLPSGTKWWQRYQPQDYRLIDNPLGD
TFDFKRMVECLTELNIWVYVDVVFNHMANESDIRADLEYPNQWDREDYAKDPEKYEALTLFGDLSEPLFTENDFVEAFGI
ENWNDKWEVQNGRISGGPHDPGLPTLADNEHVIAQQRAYLKALKAIGVKGFRIDAAKHMSLEHLEKVWDEEITHDIHIFG
EIITDGGATKEEYETFLSPYLQETKLGAYDFPLFNTLYQALEGEASLTSLIDPYIYGMALSPLRAITFATTHDIPNNQVF
QDLVMSEENEWLAYAYLFGRDGGIPLIYSENEISGFKNTEGNPRWKDEWCSKRMTQLIGFYHEMYGETQTQVEASDEHLV
FARGDRGLVAINKSDHNIEVNVAVKADIVWLEHTSQTYHAPQQGNLRFFIPAKSYLLFSR

Sequences:

>Translated_460_residues
MSQATQQHSDPVPIVSNVILHAFDWSYQRITEQAELIAQLGYRSVLVSPAMKSLNLPSGTKWWQRYQPQDYRLIDNPLGD
TFDFKRMVECLTELNIWVYVDVVFNHMANESDIRADLEYPNQWDREDYAKDPEKYEALTLFGDLSEPLFTENDFVEAFGI
ENWNDKWEVQNGRISGGPHDPGLPTLADNEHVIAQQRAYLKALKAIGVKGFRIDAAKHMSLEHLEKVWDEEITHDIHIFG
EIITDGGATKEEYETFLSPYLQETKLGAYDFPLFNTLYQALEGEASLTSLIDPYIYGMALSPLRAITFATTHDIPNNQVF
QDLVMSEENEWLAYAYLFGRDGGIPLIYSENEISGFKNTEGNPRWKDEWCSKRMTQLIGFYHEMYGETQTQVEASDEHLV
FARGDRGLVAINKSDHNIEVNVAVKADIVWLEHTSQTYHAPQQGNLRFFIPAKSYLLFSR
>Mature_459_residues
SQATQQHSDPVPIVSNVILHAFDWSYQRITEQAELIAQLGYRSVLVSPAMKSLNLPSGTKWWQRYQPQDYRLIDNPLGDT
FDFKRMVECLTELNIWVYVDVVFNHMANESDIRADLEYPNQWDREDYAKDPEKYEALTLFGDLSEPLFTENDFVEAFGIE
NWNDKWEVQNGRISGGPHDPGLPTLADNEHVIAQQRAYLKALKAIGVKGFRIDAAKHMSLEHLEKVWDEEITHDIHIFGE
IITDGGATKEEYETFLSPYLQETKLGAYDFPLFNTLYQALEGEASLTSLIDPYIYGMALSPLRAITFATTHDIPNNQVFQ
DLVMSEENEWLAYAYLFGRDGGIPLIYSENEISGFKNTEGNPRWKDEWCSKRMTQLIGFYHEMYGETQTQVEASDEHLVF
ARGDRGLVAINKSDHNIEVNVAVKADIVWLEHTSQTYHAPQQGNLRFFIPAKSYLLFSR

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Drosophila melanogaster, GI17137384, Length=449, Percent_Identity=26.7260579064588, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24654362, Length=443, Percent_Identity=25.7336343115124, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI18859631, Length=443, Percent_Identity=25.7336343115124, Blast_Score=93, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006046
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase [H]

EC number: =3.2.1.1 [H]

Molecular weight: Translated: 52889; Mature: 52757

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQATQQHSDPVPIVSNVILHAFDWSYQRITEQAELIAQLGYRSVLVSPAMKSLNLPSGT
CCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCC
KWWQRYQPQDYRLIDNPLGDTFDFKRMVECLTELNIWVYVDVVFNHMANESDIRADLEYP
HHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHCCEEEEHHHHHHHHCCCCCCEECCCCC
NQWDREDYAKDPEKYEALTLFGDLSEPLFTENDFVEAFGIENWNDKWEVQNGRISGGPHD
CCCCCHHHCCCHHHHEEEEEEECCCCCCCCCCCHHHHCCCCCCCCCEEECCCEECCCCCC
PGLPTLADNEHVIAQQRAYLKALKAIGVKGFRIDAAKHMSLEHLEKVWDEEITHDIHIFG
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECHHHHCCHHHHHHHHHHHHCCHHEEEE
EIITDGGATKEEYETFLSPYLQETKLGAYDFPLFNTLYQALEGEASLTSLIDPYIYGMAL
HHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
SPLRAITFATTHDIPNNQVFQDLVMSEENEWLAYAYLFGRDGGIPLIYSENEISGFKNTE
CCHHHEEEEEECCCCCHHHHHHHHHCCCCCEEEEEEEEECCCCEEEEEECCCCCCCCCCC
GNPRWKDEWCSKRMTQLIGFYHEMYGETQTQVEASDEHLVFARGDRGLVAINKSDHNIEV
CCCCCHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCCEEEEEECCCCEEEEECCCCEEEE
NVAVKADIVWLEHTSQTYHAPQQGNLRFFIPAKSYLLFSR
EEEEEEEEEEEECCCCCCCCCCCCCEEEEECCCCEEEECC
>Mature Secondary Structure 
SQATQQHSDPVPIVSNVILHAFDWSYQRITEQAELIAQLGYRSVLVSPAMKSLNLPSGT
CCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCC
KWWQRYQPQDYRLIDNPLGDTFDFKRMVECLTELNIWVYVDVVFNHMANESDIRADLEYP
HHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHCCEEEEHHHHHHHHCCCCCCEECCCCC
NQWDREDYAKDPEKYEALTLFGDLSEPLFTENDFVEAFGIENWNDKWEVQNGRISGGPHD
CCCCCHHHCCCHHHHEEEEEEECCCCCCCCCCCHHHHCCCCCCCCCEEECCCEECCCCCC
PGLPTLADNEHVIAQQRAYLKALKAIGVKGFRIDAAKHMSLEHLEKVWDEEITHDIHIFG
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECHHHHCCHHHHHHHHHHHHCCHHEEEE
EIITDGGATKEEYETFLSPYLQETKLGAYDFPLFNTLYQALEGEASLTSLIDPYIYGMAL
HHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
SPLRAITFATTHDIPNNQVFQDLVMSEENEWLAYAYLFGRDGGIPLIYSENEISGFKNTE
CCHHHEEEEEECCCCCHHHHHHHHHCCCCCEEEEEEEEECCCCEEEEEECCCCCCCCCCC
GNPRWKDEWCSKRMTQLIGFYHEMYGETQTQVEASDEHLVFARGDRGLVAINKSDHNIEV
CCCCCHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCCEEEEEECCCCEEEEECCCCEEEE
NVAVKADIVWLEHTSQTYHAPQQGNLRFFIPAKSYLLFSR
EEEEEEEEEEEECCCCCCCCCCCCCEEEEECCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2449422 [H]