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The map label for this gene is yghU [H]

Identifier: 59711690

GI number: 59711690

Start: 1193424

End: 1194284

Strand: Direct

Name: yghU [H]

Synonym: VF_1083

Alternate gene names: 59711690

Gene position: 1193424-1194284 (Clockwise)

Preceding gene: 59711689

Following gene: 59711691

Centisome position: 41.19

GC content: 38.56

Gene sequence:

>861_bases
ATGGAACAACAATATATCCCACCTAAAGTGTGGTCCTTTGATAGTGAAAGCGGTGGTGAATGGGCGAAAATTAATCGTCC
GGTTTCAGGTGCTACACATAAAAAGCAGCTACCAGTCGGCAAACATTATTTACAACTTTATTCGATGGGGACACCAAACG
GACAAAAAGTGACAATCATGCTTGAAGAGTTGCTAGAGCTAGGAATAAAGAAAGCGGAATATGATGCGCATTTAATTAAA
ATTAGTGAAGGCGATCAATTCTCATCTGGTTTTGTTAATGTAAACCCTAACTCTAAAATTCCAGCTCTTGTTGATTCTTC
TGGAGAAGAGCCAATAGAGGTCTTTGAATCTGGCTCTATCTTGTTATATTTAGCAGAAAAATTTAATGCGTTTATACCAG
AAGCACCTGAAAAGCGCGTAAAAGTGATGAACTGGTTATTTTGGTTACATGGTTCTGCACCATATCTTGGTGGTGGTTTT
GGGCATTTTTATGCTTATGCACCAGAGAAATTTGAATACCCAATTAACCGTTTTACGATGGAAGTAAAACGTCAGCTAGA
TGTATTAGATAAGCAGTTAGCTAAGAATCAATATATTGCGGGAGATGAATATACTATTGCAGATATGGCTATCTGGCCGT
GGTATGGCAATTTGATTCTTGGTTATTTATATAATGCTGAAACGTTTTTGGATGTAGGTAGTTATGTTCATGTGAGACGT
TGGGCGAAAATGATATTAGCGAGAACTGCTGTCCAGCGTGGACGTGTTGTGAATAGAACATGGGGAGAACCATGGGAGCA
AGTTCCAGAGCGTCATAGTGATAAAGATATTACTGAAGTTTTAGCGCTTAAACCTGAATAA

Upstream 100 bases:

>100_bases
CTAATCGTTCTCTTTATCCGCAAAATTTTGCCAAAAGCGGAAGTGCTAAACACTAATATTATTTTTATAAAAATAGTTAT
ATCGTTTTTTAGGAGTCAGT

Downstream 100 bases:

>100_bases
CTTATGGTTACTATAGTTAAGCCAAGGATTTGATGTGAGGCATTAAATCCTTGGCTTTTTAGATTTAATATATAGATAAG
ACAACTAATCATTGTTGAGA

Product: putative glutathione S-transferase YghU

Products: HX; R-S-glutathione

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MEQQYIPPKVWSFDSESGGEWAKINRPVSGATHKKQLPVGKHYLQLYSMGTPNGQKVTIMLEELLELGIKKAEYDAHLIK
ISEGDQFSSGFVNVNPNSKIPALVDSSGEEPIEVFESGSILLYLAEKFNAFIPEAPEKRVKVMNWLFWLHGSAPYLGGGF
GHFYAYAPEKFEYPINRFTMEVKRQLDVLDKQLAKNQYIAGDEYTIADMAIWPWYGNLILGYLYNAETFLDVGSYVHVRR
WAKMILARTAVQRGRVVNRTWGEPWEQVPERHSDKDITEVLALKPE

Sequences:

>Translated_286_residues
MEQQYIPPKVWSFDSESGGEWAKINRPVSGATHKKQLPVGKHYLQLYSMGTPNGQKVTIMLEELLELGIKKAEYDAHLIK
ISEGDQFSSGFVNVNPNSKIPALVDSSGEEPIEVFESGSILLYLAEKFNAFIPEAPEKRVKVMNWLFWLHGSAPYLGGGF
GHFYAYAPEKFEYPINRFTMEVKRQLDVLDKQLAKNQYIAGDEYTIADMAIWPWYGNLILGYLYNAETFLDVGSYVHVRR
WAKMILARTAVQRGRVVNRTWGEPWEQVPERHSDKDITEVLALKPE
>Mature_286_residues
MEQQYIPPKVWSFDSESGGEWAKINRPVSGATHKKQLPVGKHYLQLYSMGTPNGQKVTIMLEELLELGIKKAEYDAHLIK
ISEGDQFSSGFVNVNPNSKIPALVDSSGEEPIEVFESGSILLYLAEKFNAFIPEAPEKRVKVMNWLFWLHGSAPYLGGGF
GHFYAYAPEKFEYPINRFTMEVKRQLDVLDKQLAKNQYIAGDEYTIADMAIWPWYGNLILGYLYNAETFLDVGSYVHVRR
WAKMILARTAVQRGRVVNRTWGEPWEQVPERHSDKDITEVLALKPE

Specific function: Unknown

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI87082195, Length=276, Percent_Identity=68.8405797101449, Blast_Score=394, Evalue=1e-111,
Organism=Escherichia coli, GI1788640, Length=216, Percent_Identity=40.2777777777778, Blast_Score=144, Evalue=8e-36,
Organism=Saccharomyces cerevisiae, GI6324100, Length=248, Percent_Identity=27.0161290322581, Blast_Score=80, Evalue=5e-16,
Organism=Drosophila melanogaster, GI17864592, Length=238, Percent_Identity=27.3109243697479, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI28571670, Length=173, Percent_Identity=28.3236994219653, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI17864598, Length=201, Percent_Identity=29.3532338308458, Blast_Score=68, Evalue=8e-12,
Organism=Drosophila melanogaster, GI17864594, Length=213, Percent_Identity=26.7605633802817, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: 2.5.1.18

Molecular weight: Translated: 32707; Mature: 32707

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQQYIPPKVWSFDSESGGEWAKINRPVSGATHKKQLPVGKHYLQLYSMGTPNGQKVTIM
CCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHEEECCCCCCCEEEEE
LEELLELGIKKAEYDAHLIKISEGDQFSSGFVNVNPNSKIPALVDSSGEEPIEVFESGSI
HHHHHHHCCCCCCCCEEEEEEECCCCCCCCEEEECCCCCCCEEECCCCCCHHHHHCCCCE
LLYLAEKFNAFIPEAPEKRVKVMNWLFWLHGSAPYLGGGFGHFYAYAPEKFEYPINRFTM
EEEEEHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHEEECCCHHCCCHHHHHH
EVKRQLDVLDKQLAKNQYIAGDEYTIADMAIWPWYGNLILGYLYNAETFLDVGSYVHVRR
HHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCHHHHHHHHHHHCCHHHHHHHHHHHHHH
WAKMILARTAVQRGRVVNRTWGEPWEQVPERHSDKDITEVLALKPE
HHHHHHHHHHHHHCCEECCCCCCHHHHHHHCCCCCCHHHHHCCCCC
>Mature Secondary Structure
MEQQYIPPKVWSFDSESGGEWAKINRPVSGATHKKQLPVGKHYLQLYSMGTPNGQKVTIM
CCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHEEECCCCCCCEEEEE
LEELLELGIKKAEYDAHLIKISEGDQFSSGFVNVNPNSKIPALVDSSGEEPIEVFESGSI
HHHHHHHCCCCCCCCEEEEEEECCCCCCCCEEEECCCCCCCEEECCCCCCHHHHHCCCCE
LLYLAEKFNAFIPEAPEKRVKVMNWLFWLHGSAPYLGGGFGHFYAYAPEKFEYPINRFTM
EEEEEHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHEEECCCHHCCCHHHHHH
EVKRQLDVLDKQLAKNQYIAGDEYTIADMAIWPWYGNLILGYLYNAETFLDVGSYVHVRR
HHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCHHHHHHHHHHHCCHHHHHHHHHHHHHH
WAKMILARTAVQRGRVVNRTWGEPWEQVPERHSDKDITEVLALKPE
HHHHHHHHHHHHHCCEECCCCCCHHHHHHHCCCCCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: RX; glutathione

Specific reaction: RX + glutathione = HX + R-S-glutathione

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]