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The map label for this gene is yfcG [H]

Identifier: 59711689

GI number: 59711689

Start: 1192687

End: 1193379

Strand: Direct

Name: yfcG [H]

Synonym: VF_1082

Alternate gene names: 59711689

Gene position: 1192687-1193379 (Clockwise)

Preceding gene: 59711687

Following gene: 59711690

Centisome position: 41.16

GC content: 40.12

Gene sequence:

>693_bases
ATGCTTAAGTTTTATTTTCATCAAACACCTAACCCAATGAAAATTGCATTATATTTAGCTGAAACTGAATTGCCGTTTGA
GTTAATGCCCGTAGATACATTAAAAGGTGAGCAGCATACAAAGGAATACCGAGCGATTAATCCAAATGGGAAAGCACCTG
CAATTGTTGATGATGGAGTTCGTGTTTTTGATTCAAGTGCGATTTTACTTTACCTATCAGAAACGAGAGGAAAGCTTGGT
GGCAAAGCAGAAGACCGCGCCGAGATGCTTTCATGGCTATTATTTATCGCTTCGGGTCTAGGTCCATTTTCCGGTCAGTC
GGTACATTTCCGTCATTTTGCACCTGAAAAATTACCCTATGCAATCAACCGCTATTTATCTGAAGCACAGCGTCACTATG
ACGTTTTGGATAAACACCTTGAAGGTCGTGAATATATTGTCGGTGATGAGTTTAGCATTGCGGATATTTCGGCATGGGGT
TGGATTGATAAAGCGACAGTTGTGTTAGGTGAAGAGGGATTAGCTTCTTACCCAAATATTCAACGTTGGTTTGATGGTAT
CAATAGTCGTCCTGCTGTTGAAAAAGCACGGAATATAGCAAACAACGTTGAGTTTAAATCAGAGTTTGATGAAGAGGCTA
ATCGTTCTCTTTATCCGCAAAATTTTGCCAAAAGCGGAAGTGCTAAACACTAA

Upstream 100 bases:

>100_bases
TATCTTGAACGATCGTTCAAGTCTAGTTATATTGTTTTTAGCCCAACGAATACCAGTCGGTTGTAATGCTGGTTTATTTT
GTCATTAGAAGGATATCAAT

Downstream 100 bases:

>100_bases
TATTATTTTTATAAAAATAGTTATATCGTTTTTTAGGAGTCAGTATGGAACAACAATATATCCCACCTAAAGTGTGGTCC
TTTGATAGTGAAAGCGGTGG

Product: glutathione S-transferase

Products: HX; R-S-glutathione

Alternate protein names: GST-like protein yfcG [H]

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MLKFYFHQTPNPMKIALYLAETELPFELMPVDTLKGEQHTKEYRAINPNGKAPAIVDDGVRVFDSSAILLYLSETRGKLG
GKAEDRAEMLSWLLFIASGLGPFSGQSVHFRHFAPEKLPYAINRYLSEAQRHYDVLDKHLEGREYIVGDEFSIADISAWG
WIDKATVVLGEEGLASYPNIQRWFDGINSRPAVEKARNIANNVEFKSEFDEEANRSLYPQNFAKSGSAKH

Sequences:

>Translated_230_residues
MLKFYFHQTPNPMKIALYLAETELPFELMPVDTLKGEQHTKEYRAINPNGKAPAIVDDGVRVFDSSAILLYLSETRGKLG
GKAEDRAEMLSWLLFIASGLGPFSGQSVHFRHFAPEKLPYAINRYLSEAQRHYDVLDKHLEGREYIVGDEFSIADISAWG
WIDKATVVLGEEGLASYPNIQRWFDGINSRPAVEKARNIANNVEFKSEFDEEANRSLYPQNFAKSGSAKH
>Mature_230_residues
MLKFYFHQTPNPMKIALYLAETELPFELMPVDTLKGEQHTKEYRAINPNGKAPAIVDDGVRVFDSSAILLYLSETRGKLG
GKAEDRAEMLSWLLFIASGLGPFSGQSVHFRHFAPEKLPYAINRYLSEAQRHYDVLDKHLEGREYIVGDEFSIADISAWG
WIDKATVVLGEEGLASYPNIQRWFDGINSRPAVEKARNIANNVEFKSEFDEEANRSLYPQNFAKSGSAKH

Specific function: Has disulfide bond reductase activity (in vitro). Has low hydroperoxidase activity with cumene hydroperoxide. Has very low glutathione-S-transferase activity (in vitro) [H]

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1788640, Length=204, Percent_Identity=45.5882352941176, Blast_Score=165, Evalue=2e-42,
Organism=Escherichia coli, GI87082195, Length=217, Percent_Identity=37.7880184331797, Blast_Score=129, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6324100, Length=238, Percent_Identity=27.7310924369748, Blast_Score=100, Evalue=2e-22,
Organism=Drosophila melanogaster, GI28571670, Length=195, Percent_Identity=28.7179487179487, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI20130115, Length=192, Percent_Identity=28.6458333333333, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI45553325, Length=191, Percent_Identity=31.413612565445, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21355859, Length=191, Percent_Identity=31.413612565445, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24645375, Length=191, Percent_Identity=31.413612565445, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19922160, Length=200, Percent_Identity=27, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21686985, Length=200, Percent_Identity=26.5, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI21355857, Length=175, Percent_Identity=29.7142857142857, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI17864594, Length=153, Percent_Identity=28.7581699346405, Blast_Score=64, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: 2.5.1.18

Molecular weight: Translated: 26029; Mature: 26029

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKFYFHQTPNPMKIALYLAETELPFELMPVDTLKGEQHTKEYRAINPNGKAPAIVDDGV
CEEEEEECCCCCEEEEEEEECCCCCEEEECCHHHCCCHHHHHHEEECCCCCCCEEECCCC
RVFDSSAILLYLSETRGKLGGKAEDRAEMLSWLLFIASGLGPFSGQSVHFRHFAPEKLPY
EEECCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCHH
AINRYLSEAQRHYDVLDKHLEGREYIVGDEFSIADISAWGWIDKATVVLGEEGLASYPNI
HHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEHHCCCCCCEEEEECCCCCCCCCCH
QRWFDGINSRPAVEKARNIANNVEFKSEFDEEANRSLYPQNFAKSGSAKH
HHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHCCCCCCC
>Mature Secondary Structure
MLKFYFHQTPNPMKIALYLAETELPFELMPVDTLKGEQHTKEYRAINPNGKAPAIVDDGV
CEEEEEECCCCCEEEEEEEECCCCCEEEECCHHHCCCHHHHHHEEECCCCCCCEEECCCC
RVFDSSAILLYLSETRGKLGGKAEDRAEMLSWLLFIASGLGPFSGQSVHFRHFAPEKLPY
EEECCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCHH
AINRYLSEAQRHYDVLDKHLEGREYIVGDEFSIADISAWGWIDKATVVLGEEGLASYPNI
HHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEHHCCCCCCEEEEECCCCCCCCCCH
QRWFDGINSRPAVEKARNIANNVEFKSEFDEEANRSLYPQNFAKSGSAKH
HHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: RX; glutathione

Specific reaction: RX + glutathione = HX + R-S-glutathione

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]