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The map label for this gene is rnfA [H]

Identifier: 59711542

GI number: 59711542

Start: 1029782

End: 1030363

Strand: Reverse

Name: rnfA [H]

Synonym: VF_0935

Alternate gene names: 59711542

Gene position: 1030363-1029782 (Counterclockwise)

Preceding gene: 59711546

Following gene: 59711541

Centisome position: 35.56

GC content: 37.11

Gene sequence:

>582_bases
ATGACTGAGTATCTCTTACTACTTATTGGTACCGTTCTAGTAAACAACTTTGTACTAGTTAAGTTCTTAGGACTTTGCCC
ATTTATGGGCGTGTCTAAAAAACTAGAAAGCGCTATTGGAATGGGATTAGCAACCACATTTGTTCTTACCTTAGCATCAG
TGTGTTCGTATCTTGTCGAAACATACATCTTGTCTCCACTAGGTATTGAATACCTCAGAACAATGAGCTTTATTCTTGTT
ATTGCAGTTGTGGTGCAATTCACCGAAATGGTTGTACACAAAACAAGCCCAACTTTGTATCGTGTATTGGGTATTTTCTT
ACCATTAATTACAACCAACTGTGCCGTTCTTGGTGTTGCTCTTCTAAATGTCACTGAAAATCATAATTTCGTAGAATCTA
TTATTTATGGCTTTGGTGCTGCCGTTGGTTTTTCTTTAGTTCTTATCTTATTTTCTGCAATGCGTGAACGAATTGCTGCT
GCCGATGTACCTTTACCTTTTAAAGGTGCTTCTATTGCGATGATCACTGCAGGTTTAATGTCTTTAGCCTTTATGGGCTT
TACTGGTTTAGTGAAATTATAA

Upstream 100 bases:

>100_bases
TTTTACTTAAAATATTAAAAACCTATTCTTTCTAAGGTTGTTATCACGCGCTTAAAGTCCGATAATAACCAGATCATCTA
TTATCATAAAAATCATTGGC

Downstream 100 bases:

>100_bases
ACATGAGTTCAATCTTAATTGCTGTTATAGCTATTGCTGCTCTTGCCCTAGTATTTGGCTTAATACTGGGGTTTGCATCA
ATTAAATTTAAAGTTGAGTC

Product: Na(+)-translocating NADH-quinone reductase subunit E

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 193; Mature: 192

Protein sequence:

>193_residues
MTEYLLLLIGTVLVNNFVLVKFLGLCPFMGVSKKLESAIGMGLATTFVLTLASVCSYLVETYILSPLGIEYLRTMSFILV
IAVVVQFTEMVVHKTSPTLYRVLGIFLPLITTNCAVLGVALLNVTENHNFVESIIYGFGAAVGFSLVLILFSAMRERIAA
ADVPLPFKGASIAMITAGLMSLAFMGFTGLVKL

Sequences:

>Translated_193_residues
MTEYLLLLIGTVLVNNFVLVKFLGLCPFMGVSKKLESAIGMGLATTFVLTLASVCSYLVETYILSPLGIEYLRTMSFILV
IAVVVQFTEMVVHKTSPTLYRVLGIFLPLITTNCAVLGVALLNVTENHNFVESIIYGFGAAVGFSLVLILFSAMRERIAA
ADVPLPFKGASIAMITAGLMSLAFMGFTGLVKL
>Mature_192_residues
TEYLLLLIGTVLVNNFVLVKFLGLCPFMGVSKKLESAIGMGLATTFVLTLASVCSYLVETYILSPLGIEYLRTMSFILVI
AVVVQFTEMVVHKTSPTLYRVLGIFLPLITTNCAVLGVALLNVTENHNFVESIIYGFGAAVGFSLVLILFSAMRERIAAA
DVPLPFKGASIAMITAGLMSLAFMGFTGLVKL

Specific function: May be part of a membrane complex involved in electron transport [H]

COG id: COG4657

COG function: function code C; Predicted NADH:ubiquinone oxidoreductase, subunit RnfA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nqrDE/rnfAE family [H]

Homologues:

Organism=Escherichia coli, GI1787914, Length=193, Percent_Identity=78.2383419689119, Blast_Score=313, Evalue=4e-87,
Organism=Escherichia coli, GI1787919, Length=171, Percent_Identity=32.7485380116959, Blast_Score=60, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003667
- InterPro:   IPR011293 [H]

Pfam domain/function: PF02508 Rnf-Nqr [H]

EC number: NA

Molecular weight: Translated: 20800; Mature: 20668

Theoretical pI: Translated: 8.04; Mature: 8.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEYLLLLIGTVLVNNFVLVKFLGLCPFMGVSKKLESAIGMGLATTFVLTLASVCSYLVE
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TYILSPLGIEYLRTMSFILVIAVVVQFTEMVVHKTSPTLYRVLGIFLPLITTNCAVLGVA
HHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
LLNVTENHNFVESIIYGFGAAVGFSLVLILFSAMRERIAAADVPLPFKGASIAMITAGLM
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
SLAFMGFTGLVKL
HHHHHHHHHHHCC
>Mature Secondary Structure 
TEYLLLLIGTVLVNNFVLVKFLGLCPFMGVSKKLESAIGMGLATTFVLTLASVCSYLVE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TYILSPLGIEYLRTMSFILVIAVVVQFTEMVVHKTSPTLYRVLGIFLPLITTNCAVLGVA
HHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
LLNVTENHNFVESIIYGFGAAVGFSLVLILFSAMRERIAAADVPLPFKGASIAMITAGLM
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
SLAFMGFTGLVKL
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA