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The map label for this gene is seqA [H]

Identifier: 59711422

GI number: 59711422

Start: 893856

End: 894398

Strand: Direct

Name: seqA [H]

Synonym: VF_0815

Alternate gene names: 59711422

Gene position: 893856-894398 (Clockwise)

Preceding gene: 59711418

Following gene: 59711423

Centisome position: 30.85

GC content: 39.96

Gene sequence:

>543_bases
ATGAAGACAATTGAAGTAGACGAAGAACTATACCGCTTTATTGCGAGCCAAACTCAACACATCGGTGAAAGTGCCTCTGA
TATTTTACGTCGTCTCTTAATGCAGTCATCACCTTCAGACCTTGCTGTTTCTGCTCCTGTTGAAGTACAGAAAAAAGGCA
TTGTAGTCAGTAAAGATGCTGGTAAAGAGGCATCGGTAGATCGCGTTAAAGCGGTGCGTACCTTATTAATCTCTGATGAA
TTCTCAGCATTAGATAAAGCGATCGATCGCTTTTTAACTGTATTATCTGAATTATATAAAATTGACTCAAAAGCTTTCTC
TGAAGCAACGGAAGTAAAAGGCCGTACTCGAGTTTATTTTGCTGACAATCAAGAGACATTAATCGCGAGCGGTAAAACGA
CAAAGCCTCGTGAAATTGTAGGTACTCCATTCTGGGTAATCACAAATACAAATACTAATCGCAAGCGCCACATGGTTGAA
TTATTAATGGAGCGAATGGGCTTCCAACATGATTTAACAGAAAAAGTGTGTGCGGCGATCTAA

Upstream 100 bases:

>100_bases
ATCATTTTTCTATCGTTGTGTTAGGTTTGTCGTAGAGTATCACGATTTTACAGGGTATAATCGTGCCTTCATAAATAGAT
TATAGAATACGAACCATATA

Downstream 100 bases:

>100_bases
TTTTTTGAGTCATAACTCAAATCATAATTTGTCACAAGGAATCGTCAAATGGCTATACATCCTCGTGCTGGGCAGAAAGC
TCAGCAAGAAGATTTACACA

Product: replication initiation regulator SeqA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 180; Mature: 180

Protein sequence:

>180_residues
MKTIEVDEELYRFIASQTQHIGESASDILRRLLMQSSPSDLAVSAPVEVQKKGIVVSKDAGKEASVDRVKAVRTLLISDE
FSALDKAIDRFLTVLSELYKIDSKAFSEATEVKGRTRVYFADNQETLIASGKTTKPREIVGTPFWVITNTNTNRKRHMVE
LLMERMGFQHDLTEKVCAAI

Sequences:

>Translated_180_residues
MKTIEVDEELYRFIASQTQHIGESASDILRRLLMQSSPSDLAVSAPVEVQKKGIVVSKDAGKEASVDRVKAVRTLLISDE
FSALDKAIDRFLTVLSELYKIDSKAFSEATEVKGRTRVYFADNQETLIASGKTTKPREIVGTPFWVITNTNTNRKRHMVE
LLMERMGFQHDLTEKVCAAI
>Mature_180_residues
MKTIEVDEELYRFIASQTQHIGESASDILRRLLMQSSPSDLAVSAPVEVQKKGIVVSKDAGKEASVDRVKAVRTLLISDE
FSALDKAIDRFLTVLSELYKIDSKAFSEATEVKGRTRVYFADNQETLIASGKTTKPREIVGTPFWVITNTNTNRKRHMVE
LLMERMGFQHDLTEKVCAAI

Specific function: Negative regulator of replication initiation. Required for sequestration, a process that blocks secondary initiation events. Could block the allosteric transition of dnaA from an inactive form to an active form. Binds hemimethylated oriC. This process has

COG id: COG3057

COG function: function code L; Negative regulator of replication initiationR

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786903, Length=183, Percent_Identity=53.0054644808743, Blast_Score=191, Evalue=2e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005621
- InterPro:   IPR010985 [H]

Pfam domain/function: PF03925 SeqA [H]

EC number: NA

Molecular weight: Translated: 20208; Mature: 20208

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTIEVDEELYRFIASQTQHIGESASDILRRLLMQSSPSDLAVSAPVEVQKKGIVVSKDA
CCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEECCCHHHHCCEEEECCC
GKEASVDRVKAVRTLLISDEFSALDKAIDRFLTVLSELYKIDSKAFSEATEVKGRTRVYF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
ADNQETLIASGKTTKPREIVGTPFWVITNTNTNRKRHMVELLMERMGFQHDLTEKVCAAI
ECCCEEEEECCCCCCCHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHHCC
>Mature Secondary Structure
MKTIEVDEELYRFIASQTQHIGESASDILRRLLMQSSPSDLAVSAPVEVQKKGIVVSKDA
CCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEECCCHHHHCCEEEECCC
GKEASVDRVKAVRTLLISDEFSALDKAIDRFLTVLSELYKIDSKAFSEATEVKGRTRVYF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
ADNQETLIASGKTTKPREIVGTPFWVITNTNTNRKRHMVELLMERMGFQHDLTEKVCAAI
ECCCEEEEECCCCCCCHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]