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The map label for this gene is nagE [H]

Identifier: 59711415

GI number: 59711415

Start: 887540

End: 889033

Strand: Direct

Name: nagE [H]

Synonym: VF_0808

Alternate gene names: 59711415

Gene position: 887540-889033 (Clockwise)

Preceding gene: 59711410

Following gene: 59711416

Centisome position: 30.63

GC content: 42.77

Gene sequence:

>1494_bases
GTGAATATTTTAGGATATTTTCAAAAAGTAGGTAAGGCACTGATGGTACCAGTTGCTACATTACCTGCAGCAGCAATTTT
AATGGGTATTGGTTACTGGATTGACCCGGTAGCTTGGGGTGGCAACAGTGCACTTGCAGCGTTTTTAATCAAAGCTGGTG
CGGCTATCATCGACAACATGTCAGTACTGTTCGCAGTAGGTGTTGCTTATGGTATGTCTAAAGATAAAGATGGTGCAGCT
GCACTTTCTGGTTTTGTTGGTTTCCTAGTTGTTACTACTCTTCTTTCACCAGGAGCAGTTGCTCAAATTCAGGGTATTGA
TCCTGCTGCAGTTCCAGCTGCATTTGGTAAAATCAATAACCAATTTGTTGGTATCCTAGTTGGTATCGTATCTGCTGAAA
TCTACAACCGTTTCTCTTCTGTAGAACTGCATAAAGCACTTGCATTCTTCTCAGGTAAGCGTCTTGTACCTATCCTAACT
TCTTTCGCAGGTATCTTAATTGCGTTTGTACTTATGTACATCTGGCCTGCAATTTACGGTGGCCTAGTACACTTTGGTGA
ATCAATCCAAGGTATGGGTTCTGTTGGTGCTGGTATCTACGCATTCTTCAACCGTCTACTTATCCCTGTTGGTCTTCACC
ACGCACTTAACTCTGTGTTCTGGTTTGACGTAGCGGGTATTAACGATATTCCTAACTTCCTAGGTGGCGCTAAGTCTATC
GCTGAAGGTACTGCAACTGTAGGTGTTACAGGTATGTACCAAGCTGGTTTCTTCCCAATCATGATGTTTGGTCTACCAGG
TGCAGCACTAGCTATCTACCACACTGCTAAAGTTGAGAACAAAGAAAAAGTTGCATCTATCATGATTGCTGCTGGTTTCG
CATCATTCTTCACTGGTGTAACAGAACCTCTAGAATTCTCATTCATGTTCCTTGCTCCTGCACTATACGTAGTTCACGCA
GCATTAACAGGTATCTCTGTATTCATCGCAGCATCTATGCAATGGATTGCAGGTTTCGGTTTCTCAGCTGGTCTTGTAGA
TATGGTACTTTCTACTCGTAACCCTCTAGCTGTTAACTGGTACATGCTAATTATCCAAGGTATCGTATTCTTCGCAATCT
ACTACTTCGTATTCCGCGCTGTAATCGTTAAGTTCAACCTTAAGACTCCAGGCCGTGAAGACGACGAAGACGAAGAAGGT
TCAACAGTTGGTGGTTCTAAAGAAACTGGTGAGCTAGCTAAACAATACCTTAAAGCTCTAGGTGGTCACGAAAACCTAGA
AAACATCGATGCTTGTATCACTCGTCTACGTCTAACTCTTAAAGACACTAGCGTAATCAGCGAGAAGCAACTTAAAGCTC
TAGGTGCAATGGGTGTGGTTAAACTTGGTTCAAACAACGTTCAAGTTATCCTTGGTCCTCTTGCTGAAATCGTAGCTGGC
GAAATGAAAAAAATCCCAGCTTCTGAAGACCTAACAGCAGTTGCACTACCATAA

Upstream 100 bases:

>100_bases
TACACTGACCCTGTTTATAACGAGTAACGAAATAAGTTTTGTGAAGAACAGGTTAAGTTATTCAAAAAAAATCTAAAATC
CTTATAGGGGGAATCTAAAG

Downstream 100 bases:

>100_bases
TTATTGAGTAATTAACTCAAAATCAACAAGCCGCTCTCTTTAGAGCGGCTTTTTTTATATAAATAACCAAACTATTTGAT
TCTATATATTGAGTATCTGC

Product: fused N-acetyl glucosamine specific PTS enzyme: IIC and IIB components

Products: NA

Alternate protein names: EIICBA-Nag; EII-Nag; N-acetylglucosamine permease IIC component; PTS system N-acetylglucosamine-specific EIIC component; N-acetylglucosamine-specific phosphotransferase enzyme IIB component; PTS system N-acetylglucosamine-specific EIIB component; N-acetylglucosamine-specific phosphotransferase enzyme IIA component; PTS system N-acetylglucosamine-specific EIIA component [H]

Number of amino acids: Translated: 497; Mature: 497

Protein sequence:

>497_residues
MNILGYFQKVGKALMVPVATLPAAAILMGIGYWIDPVAWGGNSALAAFLIKAGAAIIDNMSVLFAVGVAYGMSKDKDGAA
ALSGFVGFLVVTTLLSPGAVAQIQGIDPAAVPAAFGKINNQFVGILVGIVSAEIYNRFSSVELHKALAFFSGKRLVPILT
SFAGILIAFVLMYIWPAIYGGLVHFGESIQGMGSVGAGIYAFFNRLLIPVGLHHALNSVFWFDVAGINDIPNFLGGAKSI
AEGTATVGVTGMYQAGFFPIMMFGLPGAALAIYHTAKVENKEKVASIMIAAGFASFFTGVTEPLEFSFMFLAPALYVVHA
ALTGISVFIAASMQWIAGFGFSAGLVDMVLSTRNPLAVNWYMLIIQGIVFFAIYYFVFRAVIVKFNLKTPGREDDEDEEG
STVGGSKETGELAKQYLKALGGHENLENIDACITRLRLTLKDTSVISEKQLKALGAMGVVKLGSNNVQVILGPLAEIVAG
EMKKIPASEDLTAVALP

Sequences:

>Translated_497_residues
MNILGYFQKVGKALMVPVATLPAAAILMGIGYWIDPVAWGGNSALAAFLIKAGAAIIDNMSVLFAVGVAYGMSKDKDGAA
ALSGFVGFLVVTTLLSPGAVAQIQGIDPAAVPAAFGKINNQFVGILVGIVSAEIYNRFSSVELHKALAFFSGKRLVPILT
SFAGILIAFVLMYIWPAIYGGLVHFGESIQGMGSVGAGIYAFFNRLLIPVGLHHALNSVFWFDVAGINDIPNFLGGAKSI
AEGTATVGVTGMYQAGFFPIMMFGLPGAALAIYHTAKVENKEKVASIMIAAGFASFFTGVTEPLEFSFMFLAPALYVVHA
ALTGISVFIAASMQWIAGFGFSAGLVDMVLSTRNPLAVNWYMLIIQGIVFFAIYYFVFRAVIVKFNLKTPGREDDEDEEG
STVGGSKETGELAKQYLKALGGHENLENIDACITRLRLTLKDTSVISEKQLKALGAMGVVKLGSNNVQVILGPLAEIVAG
EMKKIPASEDLTAVALP
>Mature_497_residues
MNILGYFQKVGKALMVPVATLPAAAILMGIGYWIDPVAWGGNSALAAFLIKAGAAIIDNMSVLFAVGVAYGMSKDKDGAA
ALSGFVGFLVVTTLLSPGAVAQIQGIDPAAVPAAFGKINNQFVGILVGIVSAEIYNRFSSVELHKALAFFSGKRLVPILT
SFAGILIAFVLMYIWPAIYGGLVHFGESIQGMGSVGAGIYAFFNRLLIPVGLHHALNSVFWFDVAGINDIPNFLGGAKSI
AEGTATVGVTGMYQAGFFPIMMFGLPGAALAIYHTAKVENKEKVASIMIAAGFASFFTGVTEPLEFSFMFLAPALYVVHA
ALTGISVFIAASMQWIAGFGFSAGLVDMVLSTRNPLAVNWYMLIIQGIVFFAIYYFVFRAVIVKFNLKTPGREDDEDEEG
STVGGSKETGELAKQYLKALGGHENLENIDACITRLRLTLKDTSVISEKQLKALGAMGVVKLGSNNVQVILGPLAEIVAG
EMKKIPASEDLTAVALP

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786894, Length=498, Percent_Identity=45.1807228915663, Blast_Score=357, Evalue=1e-99,
Organism=Escherichia coli, GI1787343, Length=498, Percent_Identity=37.5502008032129, Blast_Score=293, Evalue=2e-80,
Organism=Escherichia coli, GI1787908, Length=513, Percent_Identity=33.7231968810916, Blast_Score=271, Evalue=8e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011055
- InterPro:   IPR018113
- InterPro:   IPR004719
- InterPro:   IPR001127
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535
- InterPro:   IPR010974 [H]

Pfam domain/function: PF00358 PTS_EIIA_1; PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 52523; Mature: 52523

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILGYFQKVGKALMVPVATLPAAAILMGIGYWIDPVAWGGNSALAAFLIKAGAAIIDNM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
SVLFAVGVAYGMSKDKDGAAALSGFVGFLVVTTLLSPGAVAQIQGIDPAAVPAAFGKINN
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHCC
QFVGILVGIVSAEIYNRFSSVELHKALAFFSGKRLVPILTSFAGILIAFVLMYIWPAIYG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GLVHFGESIQGMGSVGAGIYAFFNRLLIPVGLHHALNSVFWFDVAGINDIPNFLGGAKSI
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHCCHHHH
AEGTATVGVTGMYQAGFFPIMMFGLPGAALAIYHTAKVENKEKVASIMIAAGFASFFTGV
HCCCHHCCCCHHHHHCHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCC
TEPLEFSFMFLAPALYVVHAALTGISVFIAASMQWIAGFGFSAGLVDMVLSTRNPLAVNW
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHH
YMLIIQGIVFFAIYYFVFRAVIVKFNLKTPGREDDEDEEGSTVGGSKETGELAKQYLKAL
HHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHC
GGHENLENIDACITRLRLTLKDTSVISEKQLKALGAMGVVKLGSNNVQVILGPLAEIVAG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEHHHHHHHHH
EMKKIPASEDLTAVALP
HHHCCCCCCCCEEEECC
>Mature Secondary Structure
MNILGYFQKVGKALMVPVATLPAAAILMGIGYWIDPVAWGGNSALAAFLIKAGAAIIDNM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
SVLFAVGVAYGMSKDKDGAAALSGFVGFLVVTTLLSPGAVAQIQGIDPAAVPAAFGKINN
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHCC
QFVGILVGIVSAEIYNRFSSVELHKALAFFSGKRLVPILTSFAGILIAFVLMYIWPAIYG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GLVHFGESIQGMGSVGAGIYAFFNRLLIPVGLHHALNSVFWFDVAGINDIPNFLGGAKSI
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHCCHHHH
AEGTATVGVTGMYQAGFFPIMMFGLPGAALAIYHTAKVENKEKVASIMIAAGFASFFTGV
HCCCHHCCCCHHHHHCHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCC
TEPLEFSFMFLAPALYVVHAALTGISVFIAASMQWIAGFGFSAGLVDMVLSTRNPLAVNW
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHH
YMLIIQGIVFFAIYYFVFRAVIVKFNLKTPGREDDEDEEGSTVGGSKETGELAKQYLKAL
HHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHC
GGHENLENIDACITRLRLTLKDTSVISEKQLKALGAMGVVKLGSNNVQVILGPLAEIVAG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEHHHHHHHHH
EMKKIPASEDLTAVALP
HHHCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3284790; 3056518; 8905232; 9278503 [H]