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The map label for this gene is prfA [H]

Identifier: 59711375

GI number: 59711375

Start: 838376

End: 839464

Strand: Direct

Name: prfA [H]

Synonym: VF_0768

Alternate gene names: 59711375

Gene position: 838376-839464 (Clockwise)

Preceding gene: 59711374

Following gene: 59711376

Centisome position: 28.93

GC content: 43.25

Gene sequence:

>1089_bases
ATGAAAGCATCCATTCGAGTAAAATTAGAGACGCTGGTTGAGCGTTATGAAGAAGTTCAGCATTTATTAGGCGATCCTGA
TGTTATCGGAGACCAAAATAAGTTTCGTGCTTTATCTCGTGAATACTCACAGCTAGAAGAAGTTACACAATGCTTCCAAG
CGTATGAGCAAGCTCAAGAAGATCTTGTTGCAGCCCAAGAAATGGCGCAAGAAGATGATGAAGAAATGCGTGAGATGGCA
CAAGAAGAAATTAAAGATGCTAAAGAAGCTATTGAACGTTTAACTGATGAACTTCAAGTTCTTCTTCTTCCAAAAGATCC
AAACGATGAACGTAACTGTTTCTTAGAAATCCGTGCTGGTGCAGGTGGTGATGAAGCGGGTATTTTTGCTGGTAACTTAT
TCCGCATGTATTCACGTTTTGCTGAGAAAAAGGGCTGGCGCGTTGAAGTAATGAGCTCAAACGAAGCTGAACACGGTGGT
TATAAAGAAATGATCGCAAAAGTGAGCGGTGAAGGTGCATACGGTGTACTTAAGTTTGAATCAGGCGGTCACCGTGTACA
ACGTGTACCAGAAACTGAATCTCAAGGCCGTGTACACACATCGGCATGTACGGTTGCAGTTATGGCTGAAATCCCAGAAG
CGGATCTTCCTGAAATTAAAGCAGCAGACTTAAAAATTGATACTTTCCGTGCATCAGGCGCGGGTGGTCAGCACGTTAAC
ACCACGGATTCCGCAATCCGTATTACTCACTTACCAACAGGTACGGTTGTTGAATGTCAGGATGAACGTTCACAACATAA
AAACAAAGCAAAAGCGATGTCTGTTCTTGCTGCTCGTATTATTCAAGCAGAAGAAGCTCGTCGTGCTGCTGCTGTTTCAG
ATACTCGCCGTAACTTGTTAGGTTCTGGTGACCGTTCAGATCGAATCCGTACTTATAACTACCCACAAGGTCGTGTATCG
GATCACCGCATTAACTTAACGGTTTACCGTTTAAATGAAGTGATGGAAGGTGATTTGGGCTGTCTAATTGAGCCAGTTGT
TCTTGAGTACCAAGCAGACCAATTAGCTGCATTGGCTGAACAAAACTAA

Upstream 100 bases:

>100_bases
AAAAAACGGTGAAACTGAAAAATTAACAGTAATCCGAACCACTATTGGTCTAGACAACTAAGCGTAGCGTTCGCTACCAA
CAAAAAGAACAAATATTATT

Downstream 100 bases:

>100_bases
TGAGCTTGTCTATTGAAGCGTTATTAAAGCGCTCAATTCAATCTCTGACGCTTGAGGGGAGTGATTCTCCTCAAGTTGAT
GCTGCTGTTCTTCTTTGTCA

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
MKASIRVKLETLVERYEEVQHLLGDPDVIGDQNKFRALSREYSQLEEVTQCFQAYEQAQEDLVAAQEMAQEDDEEMREMA
QEEIKDAKEAIERLTDELQVLLLPKDPNDERNCFLEIRAGAGGDEAGIFAGNLFRMYSRFAEKKGWRVEVMSSNEAEHGG
YKEMIAKVSGEGAYGVLKFESGGHRVQRVPETESQGRVHTSACTVAVMAEIPEADLPEIKAADLKIDTFRASGAGGQHVN
TTDSAIRITHLPTGTVVECQDERSQHKNKAKAMSVLAARIIQAEEARRAAAVSDTRRNLLGSGDRSDRIRTYNYPQGRVS
DHRINLTVYRLNEVMEGDLGCLIEPVVLEYQADQLAALAEQN

Sequences:

>Translated_362_residues
MKASIRVKLETLVERYEEVQHLLGDPDVIGDQNKFRALSREYSQLEEVTQCFQAYEQAQEDLVAAQEMAQEDDEEMREMA
QEEIKDAKEAIERLTDELQVLLLPKDPNDERNCFLEIRAGAGGDEAGIFAGNLFRMYSRFAEKKGWRVEVMSSNEAEHGG
YKEMIAKVSGEGAYGVLKFESGGHRVQRVPETESQGRVHTSACTVAVMAEIPEADLPEIKAADLKIDTFRASGAGGQHVN
TTDSAIRITHLPTGTVVECQDERSQHKNKAKAMSVLAARIIQAEEARRAAAVSDTRRNLLGSGDRSDRIRTYNYPQGRVS
DHRINLTVYRLNEVMEGDLGCLIEPVVLEYQADQLAALAEQN
>Mature_362_residues
MKASIRVKLETLVERYEEVQHLLGDPDVIGDQNKFRALSREYSQLEEVTQCFQAYEQAQEDLVAAQEMAQEDDEEMREMA
QEEIKDAKEAIERLTDELQVLLLPKDPNDERNCFLEIRAGAGGDEAGIFAGNLFRMYSRFAEKKGWRVEVMSSNEAEHGG
YKEMIAKVSGEGAYGVLKFESGGHRVQRVPETESQGRVHTSACTVAVMAEIPEADLPEIKAADLKIDTFRASGAGGQHVN
TTDSAIRITHLPTGTVVECQDERSQHKNKAKAMSVLAARIIQAEEARRAAAVSDTRRNLLGSGDRSDRIRTYNYPQGRVS
DHRINLTVYRLNEVMEGDLGCLIEPVVLEYQADQLAALAEQN

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=292, Percent_Identity=44.5205479452055, Blast_Score=258, Evalue=5e-69,
Organism=Homo sapiens, GI34577120, Length=341, Percent_Identity=39.2961876832845, Blast_Score=227, Evalue=1e-59,
Organism=Homo sapiens, GI166795305, Length=207, Percent_Identity=43.4782608695652, Blast_Score=181, Evalue=1e-45,
Organism=Escherichia coli, GI1787462, Length=361, Percent_Identity=73.1301939058172, Blast_Score=533, Evalue=1e-153,
Organism=Escherichia coli, GI2367172, Length=335, Percent_Identity=39.4029850746269, Blast_Score=218, Evalue=6e-58,
Organism=Caenorhabditis elegans, GI17542784, Length=293, Percent_Identity=35.8361774744027, Blast_Score=184, Evalue=5e-47,
Organism=Saccharomyces cerevisiae, GI6321295, Length=307, Percent_Identity=41.042345276873, Blast_Score=240, Evalue=3e-64,
Organism=Drosophila melanogaster, GI19921226, Length=330, Percent_Identity=42.1212121212121, Blast_Score=225, Evalue=4e-59,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40404; Mature: 40404

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKASIRVKLETLVERYEEVQHLLGDPDVIGDQNKFRALSREYSQLEEVTQCFQAYEQAQE
CCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLVAAQEMAQEDDEEMREMAQEEIKDAKEAIERLTDELQVLLLPKDPNDERNCFLEIRAG
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCEEEEEECC
AGGDEAGIFAGNLFRMYSRFAEKKGWRVEVMSSNEAEHGGYKEMIAKVSGEGAYGVLKFE
CCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHCCCCCEEEEEEC
SGGHRVQRVPETESQGRVHTSACTVAVMAEIPEADLPEIKAADLKIDTFRASGAGGQHVN
CCCCCEEECCCCCCCCCEEHHHHHHHHHHHCCCCCCCCCEECCEEEEEEECCCCCCCCCC
TTDSAIRITHLPTGTVVECQDERSQHKNKAKAMSVLAARIIQAEEARRAAAVSDTRRNLL
CCCCEEEEEECCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GSGDRSDRIRTYNYPQGRVSDHRINLTVYRLNEVMEGDLGCLIEPVVLEYQADQLAALAE
CCCCCCCCCEECCCCCCCCCCCEEEEEEEEEHHHHCCCCCCEECHHHHHHCHHHHHHHHC
QN
CC
>Mature Secondary Structure
MKASIRVKLETLVERYEEVQHLLGDPDVIGDQNKFRALSREYSQLEEVTQCFQAYEQAQE
CCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLVAAQEMAQEDDEEMREMAQEEIKDAKEAIERLTDELQVLLLPKDPNDERNCFLEIRAG
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCEEEEEECC
AGGDEAGIFAGNLFRMYSRFAEKKGWRVEVMSSNEAEHGGYKEMIAKVSGEGAYGVLKFE
CCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHCCCCCEEEEEEC
SGGHRVQRVPETESQGRVHTSACTVAVMAEIPEADLPEIKAADLKIDTFRASGAGGQHVN
CCCCCEEECCCCCCCCCEEHHHHHHHHHHHCCCCCCCCCEECCEEEEEEECCCCCCCCCC
TTDSAIRITHLPTGTVVECQDERSQHKNKAKAMSVLAARIIQAEEARRAAAVSDTRRNLL
CCCCEEEEEECCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GSGDRSDRIRTYNYPQGRVSDHRINLTVYRLNEVMEGDLGCLIEPVVLEYQADQLAALAE
CCCCCCCCCEECCCCCCCCCCCEEEEEEEEEHHHHCCCCCCEECHHHHHHCHHHHHHHHC
QN
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA