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The map label for this gene is prmC [H]

Identifier: 59711376

GI number: 59711376

Start: 839464

End: 840318

Strand: Direct

Name: prmC [H]

Synonym: VF_0769

Alternate gene names: 59711376

Gene position: 839464-840318 (Clockwise)

Preceding gene: 59711375

Following gene: 59711377

Centisome position: 28.97

GC content: 38.95

Gene sequence:

>855_bases
ATGAGCTTGTCTATTGAAGCGTTATTAAAGCGCTCAATTCAATCTCTGACGCTTGAGGGGAGTGATTCTCCTCAAGTTGA
TGCTGCTGTTCTTCTTTGTCATGTGTTAGATAAGCCAAGAAGTTATCTATTAACTTGGCCTGAGAAAATAGTATCAGACG
AAGAGCTGGGTAATTTTAATGCGTTACTAGAGCGTCGTCTTGCTGGTGAACCGATTGCTTATATCGTGGGTTATCGTGAA
TTTTGGTCATTACCACTTAAGGTATCGCCAACAACATTAATCCCAAGACCTGATACCGAACGTCTGGTTGAAGTGGCATT
AGATCATTTAACACCTAATGCTCAATCCATTTTGGATTTAGGAACAGGTACAGGAGCCATTGCGTTAGCTATTGCTTCTG
AAATGCCTACTCTTAATGTGATAGGTGTCGATTATCAAGACGATGCGGTTGAGTTAGCAAAAGGGAATGCGAAAATAAAT
CACATTAATAATGTTGAATTTAGACAAGGAAGTTGGTTTGAACCTATTTCTTTGAGTGATAAGTTCGACATTATTGTGAG
TAATCCCCCTTATATTGATGGTAACGATCCTCATTTATCTGAAGGGGATGTTCGTTTTGAACCGCAAACCGCTTTGGTTG
CAGAGCAAAATGGTTTTTCAGATTTAATTCACATTATGCAACACGGCCGAGAGTATTTATTAAATGGCGGTTGGTTAATG
ATGGAGCATGGTTTTGAACAAGGTGAACAATTACGTCACTTTTTTGAAGAGCATGGTTATATTAATGTGAAAACAGAGCA
AGATTATGCTGGCAATGACCGAGTTACTTTAGGTCAATGGGTAGTAGATAACTAG

Upstream 100 bases:

>100_bases
GTTTAAATGAAGTGATGGAAGGTGATTTGGGCTGTCTAATTGAGCCAGTTGTTCTTGAGTACCAAGCAGACCAATTAGCT
GCATTGGCTGAACAAAACTA

Downstream 100 bases:

>100_bases
AATAAAGGATAATTCATGTATACCGCAGTTAAACACATACACTTATTATTAGTCGCTTGTAGCGTATTGTTTTTTGTCGT
TCGCTATTGTTTGATGATGG

Product: N5-glutamine methyltransferase, modifies release factors RF-1 and RF-2

Products: NA

Alternate protein names: M.VchAHemK2P [H]

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MSLSIEALLKRSIQSLTLEGSDSPQVDAAVLLCHVLDKPRSYLLTWPEKIVSDEELGNFNALLERRLAGEPIAYIVGYRE
FWSLPLKVSPTTLIPRPDTERLVEVALDHLTPNAQSILDLGTGTGAIALAIASEMPTLNVIGVDYQDDAVELAKGNAKIN
HINNVEFRQGSWFEPISLSDKFDIIVSNPPYIDGNDPHLSEGDVRFEPQTALVAEQNGFSDLIHIMQHGREYLLNGGWLM
MEHGFEQGEQLRHFFEEHGYINVKTEQDYAGNDRVTLGQWVVDN

Sequences:

>Translated_284_residues
MSLSIEALLKRSIQSLTLEGSDSPQVDAAVLLCHVLDKPRSYLLTWPEKIVSDEELGNFNALLERRLAGEPIAYIVGYRE
FWSLPLKVSPTTLIPRPDTERLVEVALDHLTPNAQSILDLGTGTGAIALAIASEMPTLNVIGVDYQDDAVELAKGNAKIN
HINNVEFRQGSWFEPISLSDKFDIIVSNPPYIDGNDPHLSEGDVRFEPQTALVAEQNGFSDLIHIMQHGREYLLNGGWLM
MEHGFEQGEQLRHFFEEHGYINVKTEQDYAGNDRVTLGQWVVDN
>Mature_283_residues
SLSIEALLKRSIQSLTLEGSDSPQVDAAVLLCHVLDKPRSYLLTWPEKIVSDEELGNFNALLERRLAGEPIAYIVGYREF
WSLPLKVSPTTLIPRPDTERLVEVALDHLTPNAQSILDLGTGTGAIALAIASEMPTLNVIGVDYQDDAVELAKGNAKINH
INNVEFRQGSWFEPISLSDKFDIIVSNPPYIDGNDPHLSEGDVRFEPQTALVAEQNGFSDLIHIMQHGREYLLNGGWLMM
EHGFEQGEQLRHFFEEHGYINVKTEQDYAGNDRVTLGQWVVDN

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=271, Percent_Identity=29.8892988929889, Blast_Score=88, Evalue=8e-18,
Organism=Escherichia coli, GI1787463, Length=263, Percent_Identity=53.2319391634981, Blast_Score=280, Evalue=8e-77,
Organism=Escherichia coli, GI87082085, Length=269, Percent_Identity=27.1375464684015, Blast_Score=79, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324266, Length=176, Percent_Identity=30.1136363636364, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24582226, Length=240, Percent_Identity=27.5, Blast_Score=92, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 31750; Mature: 31619

Theoretical pI: Translated: 4.34; Mature: 4.34

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLSIEALLKRSIQSLTLEGSDSPQVDAAVLLCHVLDKPRSYLLTWPEKIVSDEELGNFN
CCCCHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHCCCCCEEEECHHHHCCCCCCCCHH
ALLERRLAGEPIAYIVGYREFWSLPLKVSPTTLIPRPDTERLVEVALDHLTPNAQSILDL
HHHHHHHCCCCEEEEEECHHHHCCCEEECCCEECCCCCHHHHHHHHHHHCCCCHHHHHCC
GTGTGAIALAIASEMPTLNVIGVDYQDDAVELAKGNAKINHINNVEFRQGSWFEPISLSD
CCCCCEEEEEEECCCCEEEEEEECCCCCHHHHHCCCEEEEECCCCEECCCCCCCCCCCCC
KFDIIVSNPPYIDGNDPHLSEGDVRFEPQTALVAEQNGFSDLIHIMQHGREYLLNGGWLM
CEEEEECCCCEECCCCCCCCCCCEEECCCEEEEECCCCHHHHHHHHHCCHHEEECCCEEE
MEHGFEQGEQLRHFFEEHGYINVKTEQDYAGNDRVTLGQWVVDN
EECCCCHHHHHHHHHHHCCEEEEEECCCCCCCCCEEECCEEECC
>Mature Secondary Structure 
SLSIEALLKRSIQSLTLEGSDSPQVDAAVLLCHVLDKPRSYLLTWPEKIVSDEELGNFN
CCCHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHCCCCCEEEECHHHHCCCCCCCCHH
ALLERRLAGEPIAYIVGYREFWSLPLKVSPTTLIPRPDTERLVEVALDHLTPNAQSILDL
HHHHHHHCCCCEEEEEECHHHHCCCEEECCCEECCCCCHHHHHHHHHHHCCCCHHHHHCC
GTGTGAIALAIASEMPTLNVIGVDYQDDAVELAKGNAKINHINNVEFRQGSWFEPISLSD
CCCCCEEEEEEECCCCEEEEEEECCCCCHHHHHCCCEEEEECCCCEECCCCCCCCCCCCC
KFDIIVSNPPYIDGNDPHLSEGDVRFEPQTALVAEQNGFSDLIHIMQHGREYLLNGGWLM
CEEEEECCCCEECCCCCCCCCCCEEECCCEEEEECCCCHHHHHHHHHCCHHEEECCCEEE
MEHGFEQGEQLRHFFEEHGYINVKTEQDYAGNDRVTLGQWVVDN
EECCCCHHHHHHHHHHHCCEEEEEECCCCCCCCCEEECCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]