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The map label for this gene is ubiF [H]

Identifier: 59711367

GI number: 59711367

Start: 829164

End: 830321

Strand: Direct

Name: ubiF [H]

Synonym: VF_0760

Alternate gene names: 59711367

Gene position: 829164-830321 (Clockwise)

Preceding gene: 59711361

Following gene: 59711374

Centisome position: 28.62

GC content: 37.39

Gene sequence:

>1158_bases
ATGATAAAAGAATATGATTATGCCGTAGTCGGTGGTGGCATGGTTGGCGCAGCAACTGCGGTTGGTTTGGCTAAATTAGG
TCATTCAGTTGTTTTACTTGAAGCAAGAGAACCAGCGATTTATGACAAAGCACAACCGATGGATATTCGAGTTTCGGCTA
TTTCACAATCATCAGTCGACTTATTAGATTCTTTAGGCGCTTGGAAATTTATAAGCGAAACAAGAGTATGTCCTTATAAG
CGGTTAGAAACATGGGAACATCCTGAGTGTAGGACACGTTTTCATTCAGATGCTCTTAATATGGATCAACTTGGCTTTAT
TGTTGAAAACCGATTAATCCAATTAGGTCTTTGGAATGAATTTTCTCAGTACAGTAACTTAAATGTACTTACTCCAATAA
GCATTAATACAATCACTTTTCATCAAGAATACAATGAGTTAACTCTAAGTGATAATTCCGCATTAAAAGCAAAATGGGTA
ATTGGAGCTGATGGCGCTAACTCTTATGTTCGTCAACAAGCAAAAATAGGAATAACTGCTTGGGATTATCGTCAACATTG
TATGTTGATTAATATTGAAACCGAGCAAGAACAGCAAGACATTACATGGCAATGGTTTACACCGTCAGGGCCAAGATCAT
TCTTACCTTTATTTGGAAATAAAGGCTCATTAGTTTGGTATGACTCACCTACTCGTATTAAGCAATTAACTGCAATGAGT
GATACTCAATTAGAAGAACAAATCAAGCGACACTTTCCTGCAGAACTTGGTGGTTTTAAAGTTGAACGCTTTGGATCTTT
TCCTCTTACACGCAGACATGCTCAACAATATGTAAACCATCGGTGTGTGTTACTTGGTGATGCGGCACATACCATTAATC
CGCTAGCAGGGCAGGGTGTTAATTTAGGTTTTAAAGATGTAAATGTCTTTTTAGAAGAAGTCGAAAAAAATGGCGTAGAG
AGTGATAAAGCACCATCAAGATATGAAAGCAGAAGACGAACAGATAATTTACTAATGCAAACCAGTATGGACGTTTTTTA
TACCTTATTTAGCAATACACTGCCTCCGGTTAAATTATTACGTAATATTGGATTAAGAGCTGCAGAATCAGCTGGTCCAT
TAAAGATCCAAGCACTGAAGTATGCGTTAGGATTATAG

Upstream 100 bases:

>100_bases
AGTAGACCATGAAAATTATAAGGACATGCAGTGAGTGATTTCGATTACTGATAAATCAGGCTAGAATAAGGGAAAATAAT
TGATTCAATAAGGTCGATGG

Downstream 100 bases:

>100_bases
AAAATATTAGGGACAAGGGACAAGTAGGAAAGAACTGGAAACGCAGAAGGTGAGCATTAATTGGTCACCTTTTTTGTATC
TGATTAACTAATTATCCTGA

Product: 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase

Products: 2-Octaprenyl-3-Methyl-5-Hydroxy-6-Methoxy-1,4- Benzoquinol [C]

Alternate protein names: NA

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MIKEYDYAVVGGGMVGAATAVGLAKLGHSVVLLEAREPAIYDKAQPMDIRVSAISQSSVDLLDSLGAWKFISETRVCPYK
RLETWEHPECRTRFHSDALNMDQLGFIVENRLIQLGLWNEFSQYSNLNVLTPISINTITFHQEYNELTLSDNSALKAKWV
IGADGANSYVRQQAKIGITAWDYRQHCMLINIETEQEQQDITWQWFTPSGPRSFLPLFGNKGSLVWYDSPTRIKQLTAMS
DTQLEEQIKRHFPAELGGFKVERFGSFPLTRRHAQQYVNHRCVLLGDAAHTINPLAGQGVNLGFKDVNVFLEEVEKNGVE
SDKAPSRYESRRRTDNLLMQTSMDVFYTLFSNTLPPVKLLRNIGLRAAESAGPLKIQALKYALGL

Sequences:

>Translated_385_residues
MIKEYDYAVVGGGMVGAATAVGLAKLGHSVVLLEAREPAIYDKAQPMDIRVSAISQSSVDLLDSLGAWKFISETRVCPYK
RLETWEHPECRTRFHSDALNMDQLGFIVENRLIQLGLWNEFSQYSNLNVLTPISINTITFHQEYNELTLSDNSALKAKWV
IGADGANSYVRQQAKIGITAWDYRQHCMLINIETEQEQQDITWQWFTPSGPRSFLPLFGNKGSLVWYDSPTRIKQLTAMS
DTQLEEQIKRHFPAELGGFKVERFGSFPLTRRHAQQYVNHRCVLLGDAAHTINPLAGQGVNLGFKDVNVFLEEVEKNGVE
SDKAPSRYESRRRTDNLLMQTSMDVFYTLFSNTLPPVKLLRNIGLRAAESAGPLKIQALKYALGL
>Mature_385_residues
MIKEYDYAVVGGGMVGAATAVGLAKLGHSVVLLEAREPAIYDKAQPMDIRVSAISQSSVDLLDSLGAWKFISETRVCPYK
RLETWEHPECRTRFHSDALNMDQLGFIVENRLIQLGLWNEFSQYSNLNVLTPISINTITFHQEYNELTLSDNSALKAKWV
IGADGANSYVRQQAKIGITAWDYRQHCMLINIETEQEQQDITWQWFTPSGPRSFLPLFGNKGSLVWYDSPTRIKQLTAMS
DTQLEEQIKRHFPAELGGFKVERFGSFPLTRRHAQQYVNHRCVLLGDAAHTINPLAGQGVNLGFKDVNVFLEEVEKNGVE
SDKAPSRYESRRRTDNLLMQTSMDVFYTLFSNTLPPVKLLRNIGLRAAESAGPLKIQALKYALGL

Specific function: Oxygenase that introduces the hydroxyl group at carbon five of 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol resulting in the formation of 2-octaprenyl-3-methyl-5-hydroxy-6-methoxy-1,4- benzoquinol [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiH/COQ6 family [H]

Homologues:

Organism=Homo sapiens, GI32967305, Length=433, Percent_Identity=31.6397228637413, Blast_Score=179, Evalue=5e-45,
Organism=Homo sapiens, GI32967307, Length=335, Percent_Identity=30.1492537313433, Blast_Score=148, Evalue=9e-36,
Organism=Escherichia coli, GI1786883, Length=385, Percent_Identity=49.8701298701299, Blast_Score=383, Evalue=1e-107,
Organism=Escherichia coli, GI1789273, Length=392, Percent_Identity=38.7755102040816, Blast_Score=256, Evalue=2e-69,
Organism=Escherichia coli, GI1789274, Length=388, Percent_Identity=26.2886597938144, Blast_Score=139, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI25146631, Length=431, Percent_Identity=29.9303944315545, Blast_Score=187, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6321694, Length=342, Percent_Identity=26.6081871345029, Blast_Score=100, Evalue=3e-22,
Organism=Drosophila melanogaster, GI19920760, Length=431, Percent_Identity=30.3944315545244, Blast_Score=179, Evalue=3e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042
- InterPro:   IPR018168
- InterPro:   IPR010971 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.- [C]

Molecular weight: Translated: 43447; Mature: 43447

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKEYDYAVVGGGMVGAATAVGLAKLGHSVVLLEAREPAIYDKAQPMDIRVSAISQSSVD
CCCCCCEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEEECCCCHHH
LLDSLGAWKFISETRVCPYKRLETWEHPECRTRFHSDALNMDQLGFIVENRLIQLGLWNE
HHHHCCCHHHHHCCCCCCHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHCCEEEEECCHH
FSQYSNLNVLTPISINTITFHQEYNELTLSDNSALKAKWVIGADGANSYVRQQAKIGITA
HHHHCCCCEECCEEEEEEEEECCCCEEEECCCCCEEEEEEEECCCCHHHHHHHHHCCEEE
WDYRQHCMLINIETEQEQQDITWQWFTPSGPRSFLPLFGNKGSLVWYDSPTRIKQLTAMS
EECCCCEEEEEEECCCCCCCCEEEEECCCCCCCCCCEECCCCCEEEECCCHHHHHHHHCC
DTQLEEQIKRHFPAELGGFKVERFGSFPLTRRHAQQYVNHRCVLLGDAAHTINPLAGQGV
HHHHHHHHHHHCCHHHCCEEEEECCCCCCHHHHHHHHHCCEEEEEECCHHHCCCCCCCCC
NLGFKDVNVFLEEVEKNGVESDKAPSRYESRRRTDNLLMQTSMDVFYTLFSNTLPPVKLL
CCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
RNIGLRAAESAGPLKIQALKYALGL
HHCCCEECCCCCCEEEEEHHHHHCC
>Mature Secondary Structure
MIKEYDYAVVGGGMVGAATAVGLAKLGHSVVLLEAREPAIYDKAQPMDIRVSAISQSSVD
CCCCCCEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEEECCCCHHH
LLDSLGAWKFISETRVCPYKRLETWEHPECRTRFHSDALNMDQLGFIVENRLIQLGLWNE
HHHHCCCHHHHHCCCCCCHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHCCEEEEECCHH
FSQYSNLNVLTPISINTITFHQEYNELTLSDNSALKAKWVIGADGANSYVRQQAKIGITA
HHHHCCCCEECCEEEEEEEEECCCCEEEECCCCCEEEEEEEECCCCHHHHHHHHHCCEEE
WDYRQHCMLINIETEQEQQDITWQWFTPSGPRSFLPLFGNKGSLVWYDSPTRIKQLTAMS
EECCCCEEEEEEECCCCCCCCEEEEECCCCCCCCCCEECCCCCEEEECCCHHHHHHHHCC
DTQLEEQIKRHFPAELGGFKVERFGSFPLTRRHAQQYVNHRCVLLGDAAHTINPLAGQGV
HHHHHHHHHHHCCHHHCCEEEEECCCCCCHHHHHHHHHCCEEEEEECCHHHCCCCCCCCC
NLGFKDVNVFLEEVEKNGVESDKAPSRYESRRRTDNLLMQTSMDVFYTLFSNTLPPVKLL
CCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
RNIGLRAAESAGPLKIQALKYALGL
HHCCCEECCCCCCEEEEEHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: @OCTA02.txt*2-Octaprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol! [C]

Specific reaction: @OCTA02.txt*2-Octaprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol! = 2-Octaprenyl-3-Methyl-5-Hydroxy-6-Methoxy-1,4- Benzoquinol [C]

General reaction: Hydroxylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10802164 [H]