Click here to switch to the map view.

The map label for this gene is proB [H]

Identifier: 59711347

GI number: 59711347

Start: 807361

End: 808470

Strand: Direct

Name: proB [H]

Synonym: VF_0740

Alternate gene names: 59711347

Gene position: 807361-808470 (Clockwise)

Preceding gene: 59711346

Following gene: 59711348

Centisome position: 27.86

GC content: 40.72

Gene sequence:

>1110_bases
TTGAATACACAATCTCAAACCATAGTCGTTAAACTTGGTACAAGTGTACTCACCGGTGGCACATTAAAATTAGATAGAGC
ACATATGGTTGAGTTAGTTCGCCAATGTGTTCAGCTAAAAAAAGCTGGCCATCAAGTTATTGTTGTTACGTCTGGCGCAA
TTGCTGCTGGTCGTGAGCATCTTAACTACCCCGAACTACCCAAAACCATGGCTAACAAGCAGTTATTAGCTGCTGTTGGT
CAAAGTTGCTTAATTCAAGCGTGGCAAAGTTTGTTTGGTATTTATGGTGTTGACGTAGGTCAAATGCTATTAACTCGAGC
TGATTTGGATGACCGTGAGCGTTATTTAAATGCAAGAGATATGCTTCAAGCACTATTAAAAAATAATATCGTTCCTATTG
TTAATGAAAATGATGCGGTAGCAACCAATGAAATTAAAGTTGGTGATAACGATAACCTTTCTGCATTAGTCGGTATTTTA
GCGGGTGCAGATAAATTATTATTGTTAACAGACCAATCGGGTCTATTTACCGCTGATCCACGTAAAGACCCAAAAGCAGA
ACTGATCAAAGAAGTTCATACTATCGATGAAACACTACGCAAGATAGCTGGCGGAAGTGGTACAACTCTTGGTACCGGTG
GTATGGCGACGAAGCTGCAAGCTGCTGATGTGGCGCGCCGAGCGGGTATTGAAGTAATTATTGCTGCTGGTAGTGCTGAG
AATGTAATTACTGATGTGGTTAACTCTAAACCTCAAGGTACGAAGTTCTTACCTGTTGAGTGTGCTTTAGAGAGCCGTAA
ACGTTGGATCTTAGCTGGGCCTCCATCAAAAGGTAGCATTGTTATTGATGAAGGCGCAGTAAATGCGGTTCAACAAAAAG
GCAGCAGTTTATTAAGTAAAGGTATCACTGAAGTATCAGGCCATTTCGTTCGTGGTGGCGTAGCGAAAATTGTGAATACA
AAAGGTGAATTGATTGCTCGTGGTATTTCACGTTATTCAAGTGATGACTTATCTAAGATTTTAGGAAAACACAGTCAGGA
TATTTACGCAGTATTAGGCTACGAATATGGCCCTGTAGCTATCCATCGTGATGATTTAGTACTGATTTAG

Upstream 100 bases:

>100_bases
ATTTGCTTGCTAAAAGTAGTGTCAGTTTTACCAACAACGTTACGATGATTATCGTCGTAAAATTTACATCATTTATTTAA
GTAAAATAGGTAGTAGGGAA

Downstream 100 bases:

>100_bases
TAAAAGGATATATTGTGAATTTAACAACTATGGGTCAAGCAGCTAAAGCCGCTGCATTTGACTTAGCTGTTGCTCCAACA
GCGCAAAAGAATAAAGCACT

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MNTQSQTIVVKLGTSVLTGGTLKLDRAHMVELVRQCVQLKKAGHQVIVVTSGAIAAGREHLNYPELPKTMANKQLLAAVG
QSCLIQAWQSLFGIYGVDVGQMLLTRADLDDRERYLNARDMLQALLKNNIVPIVNENDAVATNEIKVGDNDNLSALVGIL
AGADKLLLLTDQSGLFTADPRKDPKAELIKEVHTIDETLRKIAGGSGTTLGTGGMATKLQAADVARRAGIEVIIAAGSAE
NVITDVVNSKPQGTKFLPVECALESRKRWILAGPPSKGSIVIDEGAVNAVQQKGSSLLSKGITEVSGHFVRGGVAKIVNT
KGELIARGISRYSSDDLSKILGKHSQDIYAVLGYEYGPVAIHRDDLVLI

Sequences:

>Translated_369_residues
MNTQSQTIVVKLGTSVLTGGTLKLDRAHMVELVRQCVQLKKAGHQVIVVTSGAIAAGREHLNYPELPKTMANKQLLAAVG
QSCLIQAWQSLFGIYGVDVGQMLLTRADLDDRERYLNARDMLQALLKNNIVPIVNENDAVATNEIKVGDNDNLSALVGIL
AGADKLLLLTDQSGLFTADPRKDPKAELIKEVHTIDETLRKIAGGSGTTLGTGGMATKLQAADVARRAGIEVIIAAGSAE
NVITDVVNSKPQGTKFLPVECALESRKRWILAGPPSKGSIVIDEGAVNAVQQKGSSLLSKGITEVSGHFVRGGVAKIVNT
KGELIARGISRYSSDDLSKILGKHSQDIYAVLGYEYGPVAIHRDDLVLI
>Mature_369_residues
MNTQSQTIVVKLGTSVLTGGTLKLDRAHMVELVRQCVQLKKAGHQVIVVTSGAIAAGREHLNYPELPKTMANKQLLAAVG
QSCLIQAWQSLFGIYGVDVGQMLLTRADLDDRERYLNARDMLQALLKNNIVPIVNENDAVATNEIKVGDNDNLSALVGIL
AGADKLLLLTDQSGLFTADPRKDPKAELIKEVHTIDETLRKIAGGSGTTLGTGGMATKLQAADVARRAGIEVIIAAGSAE
NVITDVVNSKPQGTKFLPVECALESRKRWILAGPPSKGSIVIDEGAVNAVQQKGSSLLSKGITEVSGHFVRGGVAKIVNT
KGELIARGISRYSSDDLSKILGKHSQDIYAVLGYEYGPVAIHRDDLVLI

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI21361368, Length=294, Percent_Identity=32.9931972789116, Blast_Score=131, Evalue=9e-31,
Organism=Homo sapiens, GI62912457, Length=292, Percent_Identity=32.8767123287671, Blast_Score=129, Evalue=3e-30,
Organism=Escherichia coli, GI1786437, Length=365, Percent_Identity=68.2191780821918, Blast_Score=504, Evalue=1e-144,
Organism=Caenorhabditis elegans, GI17569969, Length=270, Percent_Identity=34.0740740740741, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17569967, Length=270, Percent_Identity=34.0740740740741, Blast_Score=138, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6321822, Length=421, Percent_Identity=36.8171021377672, Blast_Score=226, Evalue=4e-60,
Organism=Saccharomyces cerevisiae, GI6320506, Length=419, Percent_Identity=35.799522673031, Blast_Score=217, Evalue=2e-57,
Organism=Drosophila melanogaster, GI21357643, Length=307, Percent_Identity=31.9218241042345, Blast_Score=124, Evalue=9e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 39328; Mature: 39328

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTQSQTIVVKLGTSVLTGGTLKLDRAHMVELVRQCVQLKKAGHQVIVVTSGAIAAGREH
CCCCCCEEEEEECCCEECCCCEEECHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHCHHH
LNYPELPKTMANKQLLAAVGQSCLIQAWQSLFGIYGVDVGQMLLTRADLDDRERYLNARD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHCCHHH
MLQALLKNNIVPIVNENDAVATNEIKVGDNDNLSALVGILAGADKLLLLTDQSGLFTADP
HHHHHHHCCCEEEECCCCCEEECEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCEECCC
RKDPKAELIKEVHTIDETLRKIAGGSGTTLGTGGMATKLQAADVARRAGIEVIIAAGSAE
CCCCHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCHHHHHHHHHHHHCCCEEEEECCCCH
NVITDVVNSKPQGTKFLPVECALESRKRWILAGPPSKGSIVIDEGAVNAVQQKGSSLLSK
HHHHHHHCCCCCCCEEEEHHHHHCCCCCEEEECCCCCCCEEEECCHHHHHHHHHHHHHHH
GITEVSGHFVRGGVAKIVNTKGELIARGISRYSSDDLSKILGKHSQDIYAVLGYEYGPVA
CHHHHCCHHHHCCHHHHHCCCHHHHHHHHHHCCHHHHHHHHCCCCCCEEEEECCCCCCEE
IHRDDLVLI
EECCCEEEC
>Mature Secondary Structure
MNTQSQTIVVKLGTSVLTGGTLKLDRAHMVELVRQCVQLKKAGHQVIVVTSGAIAAGREH
CCCCCCEEEEEECCCEECCCCEEECHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHCHHH
LNYPELPKTMANKQLLAAVGQSCLIQAWQSLFGIYGVDVGQMLLTRADLDDRERYLNARD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHCCHHH
MLQALLKNNIVPIVNENDAVATNEIKVGDNDNLSALVGILAGADKLLLLTDQSGLFTADP
HHHHHHHCCCEEEECCCCCEEECEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCEECCC
RKDPKAELIKEVHTIDETLRKIAGGSGTTLGTGGMATKLQAADVARRAGIEVIIAAGSAE
CCCCHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCHHHHHHHHHHHHCCCEEEEECCCCH
NVITDVVNSKPQGTKFLPVECALESRKRWILAGPPSKGSIVIDEGAVNAVQQKGSSLLSK
HHHHHHHCCCCCCCEEEEHHHHHCCCCCEEEECCCCCCCEEEECCHHHHHHHHHHHHHHH
GITEVSGHFVRGGVAKIVNTKGELIARGISRYSSDDLSKILGKHSQDIYAVLGYEYGPVA
CHHHHCCHHHHCCHHHHHCCCHHHHHHHHHHCCHHHHHHHHCCCCCCEEEEECCCCCCEE
IHRDDLVLI
EECCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA