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The map label for this gene is proA

Identifier: 59711348

GI number: 59711348

Start: 808500

End: 809741

Strand: Direct

Name: proA

Synonym: VF_0741

Alternate gene names: 59711348

Gene position: 808500-809741 (Clockwise)

Preceding gene: 59711347

Following gene: 59711361

Centisome position: 27.9

GC content: 40.26

Gene sequence:

>1242_bases
ATGGGTCAAGCAGCTAAAGCCGCTGCATTTGACTTAGCTGTTGCTCCAACAGCGCAAAAGAATAAAGCACTTGCAATTAT
TGCTGATGAATTAGAAGCAAATAAAGATGCGATATTAGCAGCCAATGAAAAAGACATTAAAGCCGCAGTAGAAGCAGGCA
TTTCTGAAGCGATGCAAGATCGCTTATTACTAACTGAAGAGCGTTTGGTTGGTATTGCTAATGATGTACGTAATGTTATT
AATCTGAACGATCCTGTAGGTAGTGAGATTGACAGTAAAGTACTTGAAAACGGTATGTCATTATCACGCCGCCGTGTACC
TATTGGGGTTATCGGTGTGATTTATGAAGCTCGCCCAAATGTAACCATTGATATTGCTTCTTTGTGTCTAAAAACAGGAA
ACGCAAGCATTTTACGTGGTGGGAAAGAGACGTTTTTCTCTAATATGGAGTTGGTAAAAGTTATTCAAGTTGCTTTGGAA
AAAGCAGGATTACCAGCAGCGTCTGTTCAATATATCGAAAAGCCAGATCGTGAATTAGTGTCTCAACTTCTGACGATGGA
TGATTATGTAGATATGATCATTCCACGTGGTGGTGCTGGTTTACATAAAATGTGTAAAGAAAACAGCTCTATTCCTGTGA
TCATTGGTGGCTTTGGTATTAGCCATGCGTTTGTTGATGAAAGCGCTGACCTAGAAAGAGCATTAGTTGTTGTTGATAAC
TCAAAAGCACAGCGCCCATCTGCATGTAATGCGCTAGATACTTTATTGGTTCATGAAGCGGTAGCACCACAGTTTTTAGC
ATTACTTGCTAACAATATGGCTGGTCGTGTTGAATTAGTGGCAGAGCCTAAAGCTTACGGACTATTAAAAGAGTTTGAAG
GCGTATCATTACGTGAAGCACAAGAGGGCGATTTTGATACCGAATGGCTAAGTTACACATTAGGTGTGAAAGTAGTGGCA
GATGTGAATGAAGCTGCTGCACATATGCAAAAGCATAACGCGAGTCATTCAGATACTATTTTAACGAATAATATTGAATC
AGCTGAAAAATTTATTAATACAGCTGGCTCTGCTGCAGTATATGTTAATGCATCAACTCGTTTTACTGATGGTGCTCAAT
TTGGTTTGGGTGCTGAAGTTGCAGTATCTACTCAGAAGCTTCACGCGCGTGGTCCAATGGGCTTAGAAGAGCTAACAAGC
TACAAATGGGTAGGTAAAGCGGATTATCTAATCCGTTCTTAA

Upstream 100 bases:

>100_bases
ATATTTACGCAGTATTAGGCTACGAATATGGCCCTGTAGCTATCCATCGTGATGATTTAGTACTGATTTAGTAAAAGGAT
ATATTGTGAATTTAACAACT

Downstream 100 bases:

>100_bases
TTCAATACTCTAAAGTACCTATAAACAAAAAAGGTTGACGCCAAAACGTCAACCTTTTTTATTATTCAGACCCTAGACCC
TAGACCCTAGACCCTAGACC

Product: gamma-glutamyl phosphate reductase

Products: NA

Alternate protein names: GPR; Glutamate-5-semialdehyde dehydrogenase; Glutamyl-gamma-semialdehyde dehydrogenase; GSA dehydrogenase

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MGQAAKAAAFDLAVAPTAQKNKALAIIADELEANKDAILAANEKDIKAAVEAGISEAMQDRLLLTEERLVGIANDVRNVI
NLNDPVGSEIDSKVLENGMSLSRRRVPIGVIGVIYEARPNVTIDIASLCLKTGNASILRGGKETFFSNMELVKVIQVALE
KAGLPAASVQYIEKPDRELVSQLLTMDDYVDMIIPRGGAGLHKMCKENSSIPVIIGGFGISHAFVDESADLERALVVVDN
SKAQRPSACNALDTLLVHEAVAPQFLALLANNMAGRVELVAEPKAYGLLKEFEGVSLREAQEGDFDTEWLSYTLGVKVVA
DVNEAAAHMQKHNASHSDTILTNNIESAEKFINTAGSAAVYVNASTRFTDGAQFGLGAEVAVSTQKLHARGPMGLEELTS
YKWVGKADYLIRS

Sequences:

>Translated_413_residues
MGQAAKAAAFDLAVAPTAQKNKALAIIADELEANKDAILAANEKDIKAAVEAGISEAMQDRLLLTEERLVGIANDVRNVI
NLNDPVGSEIDSKVLENGMSLSRRRVPIGVIGVIYEARPNVTIDIASLCLKTGNASILRGGKETFFSNMELVKVIQVALE
KAGLPAASVQYIEKPDRELVSQLLTMDDYVDMIIPRGGAGLHKMCKENSSIPVIIGGFGISHAFVDESADLERALVVVDN
SKAQRPSACNALDTLLVHEAVAPQFLALLANNMAGRVELVAEPKAYGLLKEFEGVSLREAQEGDFDTEWLSYTLGVKVVA
DVNEAAAHMQKHNASHSDTILTNNIESAEKFINTAGSAAVYVNASTRFTDGAQFGLGAEVAVSTQKLHARGPMGLEELTS
YKWVGKADYLIRS
>Mature_412_residues
GQAAKAAAFDLAVAPTAQKNKALAIIADELEANKDAILAANEKDIKAAVEAGISEAMQDRLLLTEERLVGIANDVRNVIN
LNDPVGSEIDSKVLENGMSLSRRRVPIGVIGVIYEARPNVTIDIASLCLKTGNASILRGGKETFFSNMELVKVIQVALEK
AGLPAASVQYIEKPDRELVSQLLTMDDYVDMIIPRGGAGLHKMCKENSSIPVIIGGFGISHAFVDESADLERALVVVDNS
KAQRPSACNALDTLLVHEAVAPQFLALLANNMAGRVELVAEPKAYGLLKEFEGVSLREAQEGDFDTEWLSYTLGVKVVAD
VNEAAAHMQKHNASHSDTILTNNIESAEKFINTAGSAAVYVNASTRFTDGAQFGLGAEVAVSTQKLHARGPMGLEELTSY
KWVGKADYLIRS

Specific function: Catalyzes the NADPH dependent reduction of L-gamma- glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate

COG id: COG0014

COG function: function code E; Gamma-glutamyl phosphate reductase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gamma-glutamyl phosphate reductase family

Homologues:

Organism=Homo sapiens, GI62912457, Length=409, Percent_Identity=36.6748166259169, Blast_Score=233, Evalue=2e-61,
Organism=Homo sapiens, GI21361368, Length=409, Percent_Identity=36.6748166259169, Blast_Score=233, Evalue=2e-61,
Organism=Escherichia coli, GI1786438, Length=413, Percent_Identity=57.1428571428571, Blast_Score=476, Evalue=1e-135,
Organism=Caenorhabditis elegans, GI17569969, Length=371, Percent_Identity=37.466307277628, Blast_Score=231, Evalue=5e-61,
Organism=Caenorhabditis elegans, GI17569967, Length=371, Percent_Identity=37.466307277628, Blast_Score=231, Evalue=7e-61,
Organism=Saccharomyces cerevisiae, GI6324899, Length=401, Percent_Identity=37.1571072319202, Blast_Score=258, Evalue=2e-69,
Organism=Drosophila melanogaster, GI21357643, Length=408, Percent_Identity=33.3333333333333, Blast_Score=231, Evalue=8e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROA_VIBF1 (Q5E6W0)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_204124.1
- ProteinModelPortal:   Q5E6W0
- SMR:   Q5E6W0
- STRING:   Q5E6W0
- GeneID:   3277391
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_0741
- NMPDR:   fig|312309.3.peg.1242
- eggNOG:   COG0014
- HOGENOM:   HBG318080
- OMA:   QYPAACN
- PhylomeDB:   Q5E6W0
- ProtClustDB:   PRK00197
- BioCyc:   VFIS312309:VF0741-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00412
- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR000965
- InterPro:   IPR020593
- InterPro:   IPR012134
- Gene3D:   G3DSA:3.40.309.10
- Gene3D:   G3DSA:3.40.605.10
- PANTHER:   PTHR11063:SF1
- PIRSF:   PIRSF000151
- TIGRFAMs:   TIGR00407

Pfam domain/function: PF00171 Aldedh; SSF53720 Aldehyde_DH/Histidinol_DH

EC number: =1.2.1.41

Molecular weight: Translated: 44230; Mature: 44099

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS01223 PROA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQAAKAAAFDLAVAPTAQKNKALAIIADELEANKDAILAANEKDIKAAVEAGISEAMQD
CCCCHHHHHEEEEECCCCCCCCEEEEEEECCCCCCCEEEECCCHHHHHHHHHHHHHHHHH
RLLLTEERLVGIANDVRNVINLNDPVGSEIDSKVLENGMSLSRRRVPIGVIGVIYEARPN
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCHHHHHCCCCEEEEEEEEECCCC
VTIDIASLCLKTGNASILRGGKETFFSNMELVKVIQVALEKAGLPAASVQYIEKPDRELV
CEEEHHHHHHHCCCHHEECCCHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHH
SQLLTMDDYVDMIIPRGGAGLHKMCKENSSIPVIIGGFGISHAFVDESADLERALVVVDN
HHHHHHHHHHHHEECCCCCHHHHHHHCCCCCCEEEECCCCCHHHHCCCCCCCEEEEEEEC
SKAQRPSACNALDTLLVHEAVAPQFLALLANNMAGRVELVAEPKAYGLLKEFEGVSLREA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHCCCCCCCC
QEGDFDTEWLSYTLGVKVVADVNEAAAHMQKHNASHSDTILTNNIESAEKFINTAGSAAV
CCCCCCHHHHHHEECEEEEECCHHHHHHHHHCCCCCCCCEEECCHHHHHHHHHCCCCEEE
YVNASTRFTDGAQFGLGAEVAVSTQKLHARGPMGLEELTSYKWVGKADYLIRS
EEECCCCCCCCCCCCCCCEEEEEHHHHHCCCCCCHHHHHCCCCCCCCCCEECC
>Mature Secondary Structure 
GQAAKAAAFDLAVAPTAQKNKALAIIADELEANKDAILAANEKDIKAAVEAGISEAMQD
CCCHHHHHEEEEECCCCCCCCEEEEEEECCCCCCCEEEECCCHHHHHHHHHHHHHHHHH
RLLLTEERLVGIANDVRNVINLNDPVGSEIDSKVLENGMSLSRRRVPIGVIGVIYEARPN
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCHHHHHCCCCEEEEEEEEECCCC
VTIDIASLCLKTGNASILRGGKETFFSNMELVKVIQVALEKAGLPAASVQYIEKPDRELV
CEEEHHHHHHHCCCHHEECCCHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHH
SQLLTMDDYVDMIIPRGGAGLHKMCKENSSIPVIIGGFGISHAFVDESADLERALVVVDN
HHHHHHHHHHHHEECCCCCHHHHHHHCCCCCCEEEECCCCCHHHHCCCCCCCEEEEEEEC
SKAQRPSACNALDTLLVHEAVAPQFLALLANNMAGRVELVAEPKAYGLLKEFEGVSLREA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHCCCCCCCC
QEGDFDTEWLSYTLGVKVVADVNEAAAHMQKHNASHSDTILTNNIESAEKFINTAGSAAV
CCCCCCHHHHHHEECEEEEECCHHHHHHHHHCCCCCCCCEEECCHHHHHHHHHCCCCEEE
YVNASTRFTDGAQFGLGAEVAVSTQKLHARGPMGLEELTSYKWVGKADYLIRS
EEECCCCCCCCCCCCCCCEEEEEHHHHHCCCCCCHHHHHCCCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA