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The map label for this gene is nqrF [H]

Identifier: 59711338

GI number: 59711338

Start: 796348

End: 797574

Strand: Direct

Name: nqrF [H]

Synonym: VF_0731

Alternate gene names: 59711338

Gene position: 796348-797574 (Clockwise)

Preceding gene: 172087677

Following gene: 59711339

Centisome position: 27.48

GC content: 40.26

Gene sequence:

>1227_bases
ATGCAAAGCATTATTCTTGGTGTAGCGATGTTTACCATTATTGTATTGGCTTTAGTATTAGTGATCTTATTCGCTAAATC
TAAGTTAGTACCATCAGGTGACATTACTATCTCTGTAAATGGCGATGCTGATAAAGCAATCATTACTCAACCAGGTGGTA
AATTACTTAGTGCTCTTGGTAGCGCTGGTATTTTCGTATCATCTGCTTGTGGTGGTGGTGGCTCTTGTGGTCAGTGTCGT
GTGAAAGTGAAATCTGGTGGTGGTGATATTCTACCTACAGAGCTTGATCACATTTCAAAAGGTGAGGCTCGCGAAGGTGA
GCGCCTAGCATGTCAAGTTTCAATGAAAACTGACATGGAAATTGAACTTCCTGAAGAAATCTTTGGTGTTAAAAAGTGGG
AATGTACTGTTATCTCTAATGATAACGAAGCTACTTTCATTAAAGAGTTAGCGCTTGCTATCCCTGAAGGTGAAGAAGTT
CCATTCCGTGCTGGTGGTTATATTCAGATTGAAGCTGAACCACACCATATTAAATATTCGGATTTTGATATTCCTGAAGA
ATATCGTGGTGACTGGGATAAGTTTAACTTATTCCGTTACGAGTCAATCGTTAATGAACATTCAATCCGTGCTTACTCTA
TGGCTTCATACCCAGAAGAGAAAGGCATTATTAAACTGAACGTTCGTATCGCGACTCCGCCGCCAAATAATCCTGATGTT
CCACCTGGTGTAATGTCTTCTTATATTTGGTCACTAAAAGCTGGTGATAAATGTACTATTTCTGGTCCATTTGGTGAGTT
CTTCGCTAAAGATACTGACAATGAAATGGTATTCATCGGTGGTGGTGCAGGTATGGCGCCAATGCGTTCTCATATCTTTG
ACCAATTATTACGTCTTAAGTCTAAGCGTAAGATGTCTTACTGGTATGGTGCACGTTCTAAGCGTGAAATGTTCTATGTT
GAAGATTTCGATGGTCTAGCGGCTGATAACGAAAACTTCGTATGGCACTGTGCACTTTCTGATCCAATGCCAGAAGATAA
CTGGGATGGTTACACAGGCTTTATCCACAATGTTCTTTATGAAAACTACTTACGTGATCATGAAGCACCTGAAGATTGTG
AATACTACATGTGTGGTCCACCAATGATGAACGCTGCTGTTATCGGCATGCTGAAAGATCTTGGTGTTGAAGATGAAAAC
ATCTTACTAGATGACTTCGGTGGTTAA

Upstream 100 bases:

>100_bases
TTGGTATTACTTTCATTACAGTAGGTTTAATGGCGTTAGGCTTTATGTCTTTCTCTGGTGTTCAACTGTAGGGTAAGCAC
CCAATAATAAGGAATAACAA

Downstream 100 bases:

>100_bases
TCTATCGAAGTAAGAAATATTGGCTGACTCAGTACTGAGTCAGCCTTTTTTCGTATTTATGGTCCGTATTTAACCTGACA
AAGTACAGGGTTATAGGTTT

Product: Na(+)-translocating NADH-quinone reductase subunit F

Products: @TETR01txt*tetrahydropteridine!; NO3-; NAD(P); Proton [C]

Alternate protein names: Na(+)-NQR subunit F; Na(+)-translocating NQR subunit F; NQR complex subunit F; NQR-1 subunit F [H]

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MQSIILGVAMFTIIVLALVLVILFAKSKLVPSGDITISVNGDADKAIITQPGGKLLSALGSAGIFVSSACGGGGSCGQCR
VKVKSGGGDILPTELDHISKGEAREGERLACQVSMKTDMEIELPEEIFGVKKWECTVISNDNEATFIKELALAIPEGEEV
PFRAGGYIQIEAEPHHIKYSDFDIPEEYRGDWDKFNLFRYESIVNEHSIRAYSMASYPEEKGIIKLNVRIATPPPNNPDV
PPGVMSSYIWSLKAGDKCTISGPFGEFFAKDTDNEMVFIGGGAGMAPMRSHIFDQLLRLKSKRKMSYWYGARSKREMFYV
EDFDGLAADNENFVWHCALSDPMPEDNWDGYTGFIHNVLYENYLRDHEAPEDCEYYMCGPPMMNAAVIGMLKDLGVEDEN
ILLDDFGG

Sequences:

>Translated_408_residues
MQSIILGVAMFTIIVLALVLVILFAKSKLVPSGDITISVNGDADKAIITQPGGKLLSALGSAGIFVSSACGGGGSCGQCR
VKVKSGGGDILPTELDHISKGEAREGERLACQVSMKTDMEIELPEEIFGVKKWECTVISNDNEATFIKELALAIPEGEEV
PFRAGGYIQIEAEPHHIKYSDFDIPEEYRGDWDKFNLFRYESIVNEHSIRAYSMASYPEEKGIIKLNVRIATPPPNNPDV
PPGVMSSYIWSLKAGDKCTISGPFGEFFAKDTDNEMVFIGGGAGMAPMRSHIFDQLLRLKSKRKMSYWYGARSKREMFYV
EDFDGLAADNENFVWHCALSDPMPEDNWDGYTGFIHNVLYENYLRDHEAPEDCEYYMCGPPMMNAAVIGMLKDLGVEDEN
ILLDDFGG
>Mature_408_residues
MQSIILGVAMFTIIVLALVLVILFAKSKLVPSGDITISVNGDADKAIITQPGGKLLSALGSAGIFVSSACGGGGSCGQCR
VKVKSGGGDILPTELDHISKGEAREGERLACQVSMKTDMEIELPEEIFGVKKWECTVISNDNEATFIKELALAIPEGEEV
PFRAGGYIQIEAEPHHIKYSDFDIPEEYRGDWDKFNLFRYESIVNEHSIRAYSMASYPEEKGIIKLNVRIATPPPNNPDV
PPGVMSSYIWSLKAGDKCTISGPFGEFFAKDTDNEMVFIGGGAGMAPMRSHIFDQLLRLKSKRKMSYWYGARSKREMFYV
EDFDGLAADNENFVWHCALSDPMPEDNWDGYTGFIHNVLYENYLRDHEAPEDCEYYMCGPPMMNAAVIGMLKDLGVEDEN
ILLDDFGG

Specific function: NQR complex catalyzes the reduction of ubiquinone-1 to ubiquinol by two successive reactions, coupled with the transport of Na(+) ions from the cytoplasm to the periplasm. The first step is catalyzed by nqrF, which accepts electrons from NADH and reduces

COG id: COG2871

COG function: function code C; Na+-transporting NADH:ubiquinone oxidoreductase, subunit NqrF

Gene ontology:

Cell location: Cell inner membrane (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6323552, Length=195, Percent_Identity=25.6410256410256, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR010205
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.5.1.34 [C]

Molecular weight: Translated: 45085; Mature: 45085

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSIILGVAMFTIIVLALVLVILFAKSKLVPSGDITISVNGDADKAIITQPGGKLLSALG
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEEEECCCHHHHHHHC
SAGIFVSSACGGGGSCGQCRVKVKSGGGDILPTELDHISKGEAREGERLACQVSMKTDME
CCCEEEEECCCCCCCCCEEEEEEECCCCCCCCCHHHHCCCCCCCCCCEEEEEEEECCCCE
IELPEEIFGVKKWECTVISNDNEATFIKELALAIPEGEEVPFRAGGYIQIEAEPHHIKYS
EECCHHHHCCCEEEEEEEECCCCHHHHHHHHHHCCCCCCCCEECCCEEEEECCCCEEEEC
DFDIPEEYRGDWDKFNLFRYESIVNEHSIRAYSMASYPEEKGIIKLNVRIATPPPNNPDV
CCCCCHHHCCCCCCEEHHHHHHHHCCCCCEEEEECCCCCCCCEEEEEEEEECCCCCCCCC
PPGVMSSYIWSLKAGDKCTISGPFGEFFAKDTDNEMVFIGGGAGMAPMRSHIFDQLLRLK
CHHHHHHHHEEECCCCEEEEECCCHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHH
SKRKMSYWYGARSKREMFYVEDFDGLAADNENFVWHCALSDPMPEDNWDGYTGFIHNVLY
HHCCCEEEECCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHH
ENYLRDHEAPEDCEYYMCGPPMMNAAVIGMLKDLGVEDENILLDDFGG
HHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCEEEECCCC
>Mature Secondary Structure
MQSIILGVAMFTIIVLALVLVILFAKSKLVPSGDITISVNGDADKAIITQPGGKLLSALG
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEEEECCCHHHHHHHC
SAGIFVSSACGGGGSCGQCRVKVKSGGGDILPTELDHISKGEAREGERLACQVSMKTDME
CCCEEEEECCCCCCCCCEEEEEEECCCCCCCCCHHHHCCCCCCCCCCEEEEEEEECCCCE
IELPEEIFGVKKWECTVISNDNEATFIKELALAIPEGEEVPFRAGGYIQIEAEPHHIKYS
EECCHHHHCCCEEEEEEEECCCCHHHHHHHHHHCCCCCCCCEECCCEEEEECCCCEEEEC
DFDIPEEYRGDWDKFNLFRYESIVNEHSIRAYSMASYPEEKGIIKLNVRIATPPPNNPDV
CCCCCHHHCCCCCCEEHHHHHHHHCCCCCEEEEECCCCCCCCEEEEEEEEECCCCCCCCC
PPGVMSSYIWSLKAGDKCTISGPFGEFFAKDTDNEMVFIGGGAGMAPMRSHIFDQLLRLK
CHHHHHHHHEEECCCCEEEEECCCHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHH
SKRKMSYWYGARSKREMFYVEDFDGLAADNENFVWHCALSDPMPEDNWDGYTGFIHNVLY
HHCCCEEEECCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHH
ENYLRDHEAPEDCEYYMCGPPMMNAAVIGMLKDLGVEDENILLDDFGG
HHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dihydropteridine; 2 nitric oxide; O2; NAD(P)H [C]

Specific reaction: NAD(P)H + dihydropteridine = NAD(P)+ @TETR01.txt*tetrahydropteridine! 2 nitric oxide + 2 O2 + NAD(P)H = 2 NO3- + NAD(P) + Proton [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA