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The map label for this gene is ispG [H]

Identifier: 59711236

GI number: 59711236

Start: 685044

End: 686162

Strand: Direct

Name: ispG [H]

Synonym: VF_0629

Alternate gene names: 59711236

Gene position: 685044-686162 (Clockwise)

Preceding gene: 59711235

Following gene: 59711237

Centisome position: 23.64

GC content: 40.3

Gene sequence:

>1119_bases
ATGCATATAGAGTCCCCAATTAAACGTCGTCAATCTACTCGCATTTATGTGGGTAATGTTCCTATTGGTGATGGTGCTCC
CATTGCCGTTCAATCAATGACGAATACACGTACTACTGATGTAGATGCCACTGTTGCTCAAATTAAGTCTCTTGAAAAAG
TAGGCGCAGATATTGTTCGAGTTTCTGTACCGACAATGGACGCTGCTGAAGCATTCAAAGTAATTAAACAACAGGTATCA
GTTCCACTGGTTGCTGATATTCATTTTGACTACCGTATTGCTCTTCAAGTTGCGGAGTATGGTGTTGATTGTTTACGTAT
TAACCCTGGTAATATTGGTAATGAACAGCGTATTCGCTCAGTAGTTGATTGCGCTCGTGATAAAAACATTCCAATTCGAA
TTGGTGTTAACGGCGGCTCATTAGAAAAAGACATTCAAGCGAAATACAAAGAACCAACAGCTGAAGCATTATTAGAATCA
GCAATGCGTCATGTTGATATTTTAGACCGTCTTAATTTTGATCAATTTAAAGTAAGTGTAAAAGCATCAGATGTTTTCCT
TGCGGTTGATTCATATCGTCTATTAGCAAAACAAATTGCTCAGCCATTACATTTAGGCATTACTGAAGCGGGTGGTGCTC
GTGCAGGTTCTGTTAAATCAGCGGTTGGTTTGGGTATGCTGTTATCTGAAGGTATCGGTGACACATTACGCATTTCTTTA
GCTGCGGATCCCGTTGAAGAGATCAAAGTTGGTTTTGATATCTTAAAATCATTGCGTATTCGTTCTCGTGGTATTAACTT
CATTGCTTGTCCTACGTGCTCTCGTCAAGAGTTCGATGTGATAGCAACAGTGAATGAGCTTGAGCAACGTTTAGAAGATT
TAATCACACCAATGGATGTCTCTCTTATTGGATGTGTAGTAAATGGCCCTGGTGAAGCTGAAGTTTCGCATATGGGTATC
GCGGGAAGTAACCGTAAGAGTGCTTTTTATGAAGACGGAGTACGTCAGAAAGAGCGCTTTGATAACGACAACATTGTTGA
TCAGCTAGAAGCAAAGATCCGCGCAAAAGCGGCAACGTTATCAAAAGAAAACCAAATTGATATCAATCAGATCGACTGA

Upstream 100 bases:

>100_bases
TGCGGTAACACTTACTTATAAAGGCAAACCAGTTGATTTATCTGTATACCCTGCAGGTAAAGTTGCACGATTAACCCTAC
CTCAATAATGAGTTGATTCC

Downstream 100 bases:

>100_bases
TTGGTAGTAGATATAAACAAACCCATTGGGAAGTAAACGTGGCTAAAACAATCCAAGCAATTCGAGGCATGAATGATTGC
CTTCCAACACAGTCTCCATT

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MHIESPIKRRQSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVDATVAQIKSLEKVGADIVRVSVPTMDAAEAFKVIKQQVS
VPLVADIHFDYRIALQVAEYGVDCLRINPGNIGNEQRIRSVVDCARDKNIPIRIGVNGGSLEKDIQAKYKEPTAEALLES
AMRHVDILDRLNFDQFKVSVKASDVFLAVDSYRLLAKQIAQPLHLGITEAGGARAGSVKSAVGLGMLLSEGIGDTLRISL
AADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQEFDVIATVNELEQRLEDLITPMDVSLIGCVVNGPGEAEVSHMGI
AGSNRKSAFYEDGVRQKERFDNDNIVDQLEAKIRAKAATLSKENQIDINQID

Sequences:

>Translated_372_residues
MHIESPIKRRQSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVDATVAQIKSLEKVGADIVRVSVPTMDAAEAFKVIKQQVS
VPLVADIHFDYRIALQVAEYGVDCLRINPGNIGNEQRIRSVVDCARDKNIPIRIGVNGGSLEKDIQAKYKEPTAEALLES
AMRHVDILDRLNFDQFKVSVKASDVFLAVDSYRLLAKQIAQPLHLGITEAGGARAGSVKSAVGLGMLLSEGIGDTLRISL
AADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQEFDVIATVNELEQRLEDLITPMDVSLIGCVVNGPGEAEVSHMGI
AGSNRKSAFYEDGVRQKERFDNDNIVDQLEAKIRAKAATLSKENQIDINQID
>Mature_372_residues
MHIESPIKRRQSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVDATVAQIKSLEKVGADIVRVSVPTMDAAEAFKVIKQQVS
VPLVADIHFDYRIALQVAEYGVDCLRINPGNIGNEQRIRSVVDCARDKNIPIRIGVNGGSLEKDIQAKYKEPTAEALLES
AMRHVDILDRLNFDQFKVSVKASDVFLAVDSYRLLAKQIAQPLHLGITEAGGARAGSVKSAVGLGMLLSEGIGDTLRISL
AADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQEFDVIATVNELEQRLEDLITPMDVSLIGCVVNGPGEAEVSHMGI
AGSNRKSAFYEDGVRQKERFDNDNIVDQLEAKIRAKAATLSKENQIDINQID

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=372, Percent_Identity=83.8709677419355, Blast_Score=647, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011005
- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 40591; Mature: 40591

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIESPIKRRQSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVDATVAQIKSLEKVGADIVR
CCCCCHHHHCCCCEEEECCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCEEE
VSVPTMDAAEAFKVIKQQVSVPLVADIHFDYRIALQVAEYGVDCLRINPGNIGNEQRIRS
EECCCCCHHHHHHHHHHHCCCCEEEEECCCEEEEEEEHHCCCEEEEECCCCCCCHHHHHH
VVDCARDKNIPIRIGVNGGSLEKDIQAKYKEPTAEALLESAMRHVDILDRLNFDQFKVSV
HHHHHCCCCCCEEEECCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEE
KASDVFLAVDSYRLLAKQIAQPLHLGITEAGGARAGSVKSAVGLGMLLSEGIGDTLRISL
EECEEEEEEHHHHHHHHHHCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEE
AADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQEFDVIATVNELEQRLEDLITPMDV
ECCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHEEEHHHHHHHHHHHCCCCCC
SLIGCVVNGPGEAEVSHMGIAGSNRKSAFYEDGVRQKERFDNDNIVDQLEAKIRAKAATL
EEEEEEECCCCCCCEEEECCCCCCCCCHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHC
SKENQIDINQID
CCCCCCCCCCCC
>Mature Secondary Structure
MHIESPIKRRQSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVDATVAQIKSLEKVGADIVR
CCCCCHHHHCCCCEEEECCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCEEE
VSVPTMDAAEAFKVIKQQVSVPLVADIHFDYRIALQVAEYGVDCLRINPGNIGNEQRIRS
EECCCCCHHHHHHHHHHHCCCCEEEEECCCEEEEEEEHHCCCEEEEECCCCCCCHHHHHH
VVDCARDKNIPIRIGVNGGSLEKDIQAKYKEPTAEALLESAMRHVDILDRLNFDQFKVSV
HHHHHCCCCCCEEEECCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEE
KASDVFLAVDSYRLLAKQIAQPLHLGITEAGGARAGSVKSAVGLGMLLSEGIGDTLRISL
EECEEEEEEHHHHHHHHHHCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEE
AADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQEFDVIATVNELEQRLEDLITPMDV
ECCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHEEEHHHHHHHHHHHCCCCCC
SLIGCVVNGPGEAEVSHMGIAGSNRKSAFYEDGVRQKERFDNDNIVDQLEAKIRAKAATL
EEEEEEECCCCCCCEEEECCCCCCCCCHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHC
SKENQIDINQID
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA