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The map label for this gene is hisS

Identifier: 59711237

GI number: 59711237

Start: 686201

End: 687469

Strand: Direct

Name: hisS

Synonym: VF_0630

Alternate gene names: 59711237

Gene position: 686201-687469 (Clockwise)

Preceding gene: 59711236

Following gene: 59711238

Centisome position: 23.68

GC content: 41.13

Gene sequence:

>1269_bases
GTGGCTAAAACAATCCAAGCAATTCGAGGCATGAATGATTGCCTTCCAACACAGTCTCCATTGTGGCAAAAAGTTGAAGG
TAGCGTAAAACGCGTTATCAGCGCATATGGTTATAACGAAGTTCGTATGCCAATTGTTGAGCAAACTCATCTATTTAAAC
GCGCTATCGGTGAAGTAACTGATGTTGTAGAAAAAGAGATGTATACGTTTGAAGACCGTAATGGCGATAGCTTGACTCTT
CGTCCAGAAGGTACAGCGGGTTGTGTTCGTGCAGGTATTGAAAATGGTTTACTGTACAACCAAGAGCAACGTTTATGGTA
CATGGGTCCAATGTTCCGTCATGAACGTCCTCAAAAAGGTCGTTACCGTCAATTCCACCAAGTAGGTGTTGAAGTTTTTG
GCTTAAATGGTCCTGATGTTGATGCTGAATTGATCATGATGACAGCTCGTTTATGGCGTGAATTAGGTATCGATCAACAC
GTTCGTTTAGAGTTGAACTCAATTGGTTCACTAGAAGCTCGTGCTAACTACCGTACAGCATTAGTTGCTTTCTTAGAGCA
GCATTTAGATGTATTAGATGAAGATTGTAAGCGTCGTATGCACACAAACCCAATGCGTGTATTAGATACTAAGAACCCTG
ATGTTCAAGCTATTTTAGGTGATGCACCTAAGCTATCAGAATACTTAGATGACGATTCTAAAGCACATTTTTCTGGATTA
TGTGAACTACTTGACGCTGCTGGCATCCAATATCAAGTTAATGAACGTTTAGTTCGCGGTTTAGATTATTATAACCGTAC
TGTTTTTGAGTGGATTACTGAAAGTCTAGGCGCTCAAGGTACAGTATGTGGTGGTGGTCGTTACGATGGTCTAGTTGAGC
AGCTTGGTGGTAAAACAACACCAGCAGTTGGCTTTGCTATGGGCTTAGAACGTCTTGTTCTTCTAATGGAAACATTAGAA
TTAACAGATGTTCGTCGTTCTGTTGACGTATACATGGTAACCGCTGGTGAAGGTACACTTATGGCTGGTATGAAACTGGC
TGAATCACTACGTGAACAAGTTCCAGGCTTACGAGTTATGTGTCACTTTGGTGGTGGTAATTTTAAAAAGCAATTTAAAC
GTGCTGATAATGCAGGTGCAGCAGTAGCCCTAATCCTAGGTGAAACTGAAGTTGCAGAGCAAACAGTTAACGTTAAAGAT
CTACGTAATGGCGAGCAAGTGACTCTGCCACAATCAGACGTATTCACAAAATTAGCTGAATTGATTTAA

Upstream 100 bases:

>100_bases
TCCGCGCAAAAGCGGCAACGTTATCAAAAGAAAACCAAATTGATATCAATCAGATCGACTGATTGGTAGTAGATATAAAC
AAACCCATTGGGAAGTAAAC

Downstream 100 bases:

>100_bases
GAGGATAGGACGTGGAAGCCTACGAAACTGAAGAACAACAAGTTGAAGCCATTAAAAGTTGGTGGAAAGAAAATGGCAAG
GCTGTCGTTATTGGTGGTGT

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 422; Mature: 421

Protein sequence:

>422_residues
MAKTIQAIRGMNDCLPTQSPLWQKVEGSVKRVISAYGYNEVRMPIVEQTHLFKRAIGEVTDVVEKEMYTFEDRNGDSLTL
RPEGTAGCVRAGIENGLLYNQEQRLWYMGPMFRHERPQKGRYRQFHQVGVEVFGLNGPDVDAELIMMTARLWRELGIDQH
VRLELNSIGSLEARANYRTALVAFLEQHLDVLDEDCKRRMHTNPMRVLDTKNPDVQAILGDAPKLSEYLDDDSKAHFSGL
CELLDAAGIQYQVNERLVRGLDYYNRTVFEWITESLGAQGTVCGGGRYDGLVEQLGGKTTPAVGFAMGLERLVLLMETLE
LTDVRRSVDVYMVTAGEGTLMAGMKLAESLREQVPGLRVMCHFGGGNFKKQFKRADNAGAAVALILGETEVAEQTVNVKD
LRNGEQVTLPQSDVFTKLAELI

Sequences:

>Translated_422_residues
MAKTIQAIRGMNDCLPTQSPLWQKVEGSVKRVISAYGYNEVRMPIVEQTHLFKRAIGEVTDVVEKEMYTFEDRNGDSLTL
RPEGTAGCVRAGIENGLLYNQEQRLWYMGPMFRHERPQKGRYRQFHQVGVEVFGLNGPDVDAELIMMTARLWRELGIDQH
VRLELNSIGSLEARANYRTALVAFLEQHLDVLDEDCKRRMHTNPMRVLDTKNPDVQAILGDAPKLSEYLDDDSKAHFSGL
CELLDAAGIQYQVNERLVRGLDYYNRTVFEWITESLGAQGTVCGGGRYDGLVEQLGGKTTPAVGFAMGLERLVLLMETLE
LTDVRRSVDVYMVTAGEGTLMAGMKLAESLREQVPGLRVMCHFGGGNFKKQFKRADNAGAAVALILGETEVAEQTVNVKD
LRNGEQVTLPQSDVFTKLAELI
>Mature_421_residues
AKTIQAIRGMNDCLPTQSPLWQKVEGSVKRVISAYGYNEVRMPIVEQTHLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLR
PEGTAGCVRAGIENGLLYNQEQRLWYMGPMFRHERPQKGRYRQFHQVGVEVFGLNGPDVDAELIMMTARLWRELGIDQHV
RLELNSIGSLEARANYRTALVAFLEQHLDVLDEDCKRRMHTNPMRVLDTKNPDVQAILGDAPKLSEYLDDDSKAHFSGLC
ELLDAAGIQYQVNERLVRGLDYYNRTVFEWITESLGAQGTVCGGGRYDGLVEQLGGKTTPAVGFAMGLERLVLLMETLEL
TDVRRSVDVYMVTAGEGTLMAGMKLAESLREQVPGLRVMCHFGGGNFKKQFKRADNAGAAVALILGETEVAEQTVNVKDL
RNGEQVTLPQSDVFTKLAELI

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI6996014, Length=465, Percent_Identity=23.2258064516129, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI15029520, Length=459, Percent_Identity=22.8758169934641, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1788861, Length=423, Percent_Identity=72.1040189125296, Blast_Score=648, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71993686, Length=463, Percent_Identity=23.9740820734341, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71993693, Length=463, Percent_Identity=23.9740820734341, Blast_Score=86, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_VIBF1 (Q5E771)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_204013.1
- ProteinModelPortal:   Q5E771
- SMR:   Q5E771
- STRING:   Q5E771
- GeneID:   3277352
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_0630
- NMPDR:   fig|312309.3.peg.1131
- eggNOG:   COG0124
- HOGENOM:   HBG616575
- OMA:   VFEWVTT
- PhylomeDB:   Q5E771
- ProtClustDB:   PRK00037
- BioCyc:   VFIS312309:VF0630-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 47238; Mature: 47107

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKTIQAIRGMNDCLPTQSPLWQKVEGSVKRVISAYGYNEVRMPIVEQTHLFKRAIGEVT
CCCHHHHHCCCHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHHHH
DVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIENGLLYNQEQRLWYMGPMFRHERPQKG
HHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHCCCEECCCCCEEEECCHHHCCCCCCC
RYRQFHQVGVEVFGLNGPDVDAELIMMTARLWRELGIDQHVRLELNSIGSLEARANYRTA
HHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHH
LVAFLEQHLDVLDEDCKRRMHTNPMRVLDTKNPDVQAILGDAPKLSEYLDDDSKAHFSGL
HHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCEEEECCCHHHHHHCCCCCHHHHHHH
CELLDAAGIQYQVNERLVRGLDYYNRTVFEWITESLGAQGTVCGGGRYDGLVEQLGGKTT
HHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHCCCCC
PAVGFAMGLERLVLLMETLELTDVRRSVDVYMVTAGEGTLMAGMKLAESLREQVPGLRVM
CHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEE
CHFGGGNFKKQFKRADNAGAAVALILGETEVAEQTVNVKDLRNGEQVTLPQSDVFTKLAE
EEECCCCHHHHHHHCCCCCCEEEEEECCHHHHHHHCCHHHCCCCCEEECCHHHHHHHHHH
LI
HC
>Mature Secondary Structure 
AKTIQAIRGMNDCLPTQSPLWQKVEGSVKRVISAYGYNEVRMPIVEQTHLFKRAIGEVT
CCHHHHHCCCHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHHHH
DVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIENGLLYNQEQRLWYMGPMFRHERPQKG
HHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHCCCEECCCCCEEEECCHHHCCCCCCC
RYRQFHQVGVEVFGLNGPDVDAELIMMTARLWRELGIDQHVRLELNSIGSLEARANYRTA
HHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHH
LVAFLEQHLDVLDEDCKRRMHTNPMRVLDTKNPDVQAILGDAPKLSEYLDDDSKAHFSGL
HHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCEEEECCCHHHHHHCCCCCHHHHHHH
CELLDAAGIQYQVNERLVRGLDYYNRTVFEWITESLGAQGTVCGGGRYDGLVEQLGGKTT
HHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHCCCCC
PAVGFAMGLERLVLLMETLELTDVRRSVDVYMVTAGEGTLMAGMKLAESLREQVPGLRVM
CHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEE
CHFGGGNFKKQFKRADNAGAAVALILGETEVAEQTVNVKDLRNGEQVTLPQSDVFTKLAE
EEECCCCHHHHHHHCCCCCCEEEEEECCHHHHHHHCCHHHCCCCCEEECCHHHHHHHHHH
LI
HC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA