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The map label for this gene is yfgB [H]

Identifier: 59711233

GI number: 59711233

Start: 682216

End: 683334

Strand: Direct

Name: yfgB [H]

Synonym: VF_0626

Alternate gene names: 59711233

Gene position: 682216-683334 (Clockwise)

Preceding gene: 59711232

Following gene: 59711234

Centisome position: 23.54

GC content: 40.13

Gene sequence:

>1119_bases
ATGACTACAGCTAAAATCAATCTACTGGACTTTGATCGTAAAGGACTTCGAGCATTTTTTTCTGAGGAATTAGGAGAGAA
AGCCTTCCGTGCAGATCAAGTAATGAAGTGGATGTATCACTTTGGTTGTGATGACTTCGATCAAATGAACAACATCAATA
AAAAGCTTCGTGAAAAACTAAAGCATAAGTGTGAAATTCGCGCACCTTACGTATCAGAAGCACAGCATTCATCTGATGGC
ACGATTAAGTGGGCGATGAAAGTTGGCGATCAAGACGTAGAAACGGTATACATCCCTGATGGTGACCGTGCAACGCTTTG
TGTATCTTCACAGGTTGGTTGTGCATTAGAATGTAAATTCTGTTCAACAGCTCAACAAGGCTTTAACCGTAACCTAAAAG
TTTCTGAGATTGTGGGTCAAATCTGGCGTGCAGCTCGTGAAATTGGTCTAGAAAAAGAAACGGGTCGTCGTCCAATTACT
AACGTCGTAATGATGGGGATGGGTGAGCCATTACTTAACATGAAAAACCTAATTCCAGCATTAGAAATTATGCTAGATGA
TTTAGGTTTTGCTCTATCTAAGCGTCGTGTAACTGTTTCTACATCAGGTGTTGTTTCTGGTCTTGACCAAATGACAGGTA
AAATTGATGTTGCATTAGCGATTTCGCTGCATGCACCAACTGATGAACTTCGTAGCCAAATTATGCCTATCAACGATCGT
TGGGATATTGATGCGTTTTTAGCTTCAGTTCGTCGTTACATTGCATCATCAAATGCAAACCGTGGTCGTGTAACGGTTGA
GTATGTTCTTCTTGATCATGTTAATGACGATATGGATCATGCAAGACAACTCGCAGAATTGCTAAAAGATACGCCTGCGA
AGATTAATTTGATTCCATTTAACCCTTATCCTGGGTCACCATATAAGAAACCAAGTAACTCACGTATTGATCGTTTCATG
AAAACCTTAATGGAATACGACTATACCGTGACAATTCGTAAAACTCGTGGCGATGATATCGATGCTGCTTGTGGTCAATT
AGTTGGTGATGTAATCGACAGAACTAAACGCACTAAAGTTAAACAGCAAGGTGAGGCAATCCCTGTAAAAACTGTTTAA

Upstream 100 bases:

>100_bases
AACCTCGAACCTCGAACCTCGAACCCCTAGTAATATCCTCCCAAAGGCTGTACAATTCTGCGCCCATATTCGCGGTGATG
TCATTTATCAGAGAGGCAAC

Downstream 100 bases:

>100_bases
TCTTGGTGTATTACAGCAAGGGAAGGCAGGATGAGAAAATGGAGTGCAGCACTATTAACGATTGGATTGTTAACAAGTGC
TGGTTGCGTCACTGTTGACG

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 372; Mature: 371

Protein sequence:

>372_residues
MTTAKINLLDFDRKGLRAFFSEELGEKAFRADQVMKWMYHFGCDDFDQMNNINKKLREKLKHKCEIRAPYVSEAQHSSDG
TIKWAMKVGDQDVETVYIPDGDRATLCVSSQVGCALECKFCSTAQQGFNRNLKVSEIVGQIWRAAREIGLEKETGRRPIT
NVVMMGMGEPLLNMKNLIPALEIMLDDLGFALSKRRVTVSTSGVVSGLDQMTGKIDVALAISLHAPTDELRSQIMPINDR
WDIDAFLASVRRYIASSNANRGRVTVEYVLLDHVNDDMDHARQLAELLKDTPAKINLIPFNPYPGSPYKKPSNSRIDRFM
KTLMEYDYTVTIRKTRGDDIDAACGQLVGDVIDRTKRTKVKQQGEAIPVKTV

Sequences:

>Translated_372_residues
MTTAKINLLDFDRKGLRAFFSEELGEKAFRADQVMKWMYHFGCDDFDQMNNINKKLREKLKHKCEIRAPYVSEAQHSSDG
TIKWAMKVGDQDVETVYIPDGDRATLCVSSQVGCALECKFCSTAQQGFNRNLKVSEIVGQIWRAAREIGLEKETGRRPIT
NVVMMGMGEPLLNMKNLIPALEIMLDDLGFALSKRRVTVSTSGVVSGLDQMTGKIDVALAISLHAPTDELRSQIMPINDR
WDIDAFLASVRRYIASSNANRGRVTVEYVLLDHVNDDMDHARQLAELLKDTPAKINLIPFNPYPGSPYKKPSNSRIDRFM
KTLMEYDYTVTIRKTRGDDIDAACGQLVGDVIDRTKRTKVKQQGEAIPVKTV
>Mature_371_residues
TTAKINLLDFDRKGLRAFFSEELGEKAFRADQVMKWMYHFGCDDFDQMNNINKKLREKLKHKCEIRAPYVSEAQHSSDGT
IKWAMKVGDQDVETVYIPDGDRATLCVSSQVGCALECKFCSTAQQGFNRNLKVSEIVGQIWRAAREIGLEKETGRRPITN
VVMMGMGEPLLNMKNLIPALEIMLDDLGFALSKRRVTVSTSGVVSGLDQMTGKIDVALAISLHAPTDELRSQIMPINDRW
DIDAFLASVRRYIASSNANRGRVTVEYVLLDHVNDDMDHARQLAELLKDTPAKINLIPFNPYPGSPYKKPSNSRIDRFMK
TLMEYDYTVTIRKTRGDDIDAACGQLVGDVIDRTKRTKVKQQGEAIPVKTV

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=370, Percent_Identity=72.4324324324324, Blast_Score=569, Evalue=1e-164,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 41916; Mature: 41785

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTAKINLLDFDRKGLRAFFSEELGEKAFRADQVMKWMYHFGCDDFDQMNNINKKLREKL
CCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
KHKCEIRAPYVSEAQHSSDGTIKWAMKVGDQDVETVYIPDGDRATLCVSSQVGCALECKF
HHHCCCCCCCHHCCCCCCCCCEEEEEECCCCCCCEEEECCCCCEEEEEECCCCCEEEEHH
CSTAQQGFNRNLKVSEIVGQIWRAAREIGLEKETGRRPITNVVMMGMGEPLLNMKNLIPA
HHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHCCCCHHHHHHHHCCCCHHHHHHHHHHH
LEIMLDDLGFALSKRRVTVSTSGVVSGLDQMTGKIDVALAISLHAPTDELRSQIMPINDR
HHHHHHHHHHHHHCCEEEEECCHHHHHHHHHCCCEEEEEEEEECCCHHHHHHHCCCCCCC
WDIDAFLASVRRYIASSNANRGRVTVEYVLLDHVNDDMDHARQLAELLKDTPAKINLIPF
CCHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEC
NPYPGSPYKKPSNSRIDRFMKTLMEYDYTVTIRKTRGDDIDAACGQLVGDVIDRTKRTKV
CCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
KQQGEAIPVKTV
HHCCCCCCCCCC
>Mature Secondary Structure 
TTAKINLLDFDRKGLRAFFSEELGEKAFRADQVMKWMYHFGCDDFDQMNNINKKLREKL
CCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
KHKCEIRAPYVSEAQHSSDGTIKWAMKVGDQDVETVYIPDGDRATLCVSSQVGCALECKF
HHHCCCCCCCHHCCCCCCCCCEEEEEECCCCCCCEEEECCCCCEEEEEECCCCCEEEEHH
CSTAQQGFNRNLKVSEIVGQIWRAAREIGLEKETGRRPITNVVMMGMGEPLLNMKNLIPA
HHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHCCCCHHHHHHHHCCCCHHHHHHHHHHH
LEIMLDDLGFALSKRRVTVSTSGVVSGLDQMTGKIDVALAISLHAPTDELRSQIMPINDR
HHHHHHHHHHHHHCCEEEEECCHHHHHHHHHCCCEEEEEEEEECCCHHHHHHHCCCCCCC
WDIDAFLASVRRYIASSNANRGRVTVEYVLLDHVNDDMDHARQLAELLKDTPAKINLIPF
CCHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEC
NPYPGSPYKKPSNSRIDRFMKTLMEYDYTVTIRKTRGDDIDAACGQLVGDVIDRTKRTKV
CCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
KQQGEAIPVKTV
HHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA