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The map label for this gene is iscS [H]

Identifier: 59711224

GI number: 59711224

Start: 674533

End: 675792

Strand: Direct

Name: iscS [H]

Synonym: VF_0617

Alternate gene names: 59711224

Gene position: 674533-675792 (Clockwise)

Preceding gene: 59711223

Following gene: 59711225

Centisome position: 23.28

GC content: 40.56

Gene sequence:

>1260_bases
ATGTCCGTTCTTAACGGTCAAACGTCTGTATTTTTGGAGATGAAAATGAAACTGCCAATTTACTTTGATTATTCGGCTAC
GTGTCCTGTTGATAAACGTGTAGCGGATAAAATGGTTCAATACATGACAATGGATGGTTACTTTGGTAACCCTGCATCAC
GCTCACACCGTTACGGCTGGCAGGCTGAAGAAGCGGTTGATACTGCACGTGAAAATATTGCCGATTTATTGAATGCTGAT
CCTCGTGAGATCGTATTTACATCGGGTGCAACAGAGTCTGACAACTTAGCAATTAAAGGTGCTGCGCATTTCTATAATAA
GAAAGGTAAGCACATCATTACTTGTAAAACAGAACATAAAGCGGTTCTTGACCCATGTCGTCAACTTGAGCGTGAAGGTT
ATGAAGTAACTTACCTTGAGCCAGAATCAAACGGCCTTATTGACCTAGCTAAACTTGAAGCAGCAATGCGTGATGACACC
GTTCTTGTTTCAATTATGCATGTAAATAACGAAATTGGTGTAATTCAAGATATTGCAGCAATTGGCGAGTTATGTCGTTC
TCGTAAAATTATTTTCCATGTTGATGCTGCACAATCTGCGGGTAAAATCCCACTAGATGTACAAGAAATTAAAGTAGATT
TAATTTCACTATCAGCTCATAAAATTTATGGCCCTAAAGGTATCGGCGCATTATATGTTCGTCGTAAGCCTCGCATTCGT
TTAGAAGCACAAATGCACGGTGGTGGTCATGAGCGTGGTTTCCGTTCTGGTACTCTAGCAACACATCAAATTGTTGGTAT
GGGTGAAGCGTGTCGTATTGCAAAAGAAGAAATGCAAAAAGACTACGATCACTGTTTAGCAATGCGTGACCGTTTATTGG
ACGGCATTAAAGATATGGAAGCGGTACACATTAATGGTGATTTAGACCAACGTGTACCAAGTAACCTGAATATCAGTTTT
GAATTTGTTGAAGGTGAGTCTTTATTAATGGCTCTTAAAGATCTTGCTGTATCTTCTGGTTCTGCATGTACTTCAGCAAG
TCTTGAACCTTCATATGTACTACGTGCATTAGGTCTAAATGATGAACTAGCACACAGTTCAATTCGTTTCTCTTTTGGCC
GTTATACAACAGCAGAAGAGATCGATCACGCGGTTTCACAAATTAGCCAAGCAGTAAACAAACTGCGTGATATGTCACCA
CTGTGGGACATGTACAAAGAAGGCATCGACTTAAACACGGTTGAGTGGGCACACCACTAA

Upstream 100 bases:

>100_bases
GATAATGAAGTTCAGCAAATATCCGATCGACAAAATGTTGATCTTGCCATAACAAATGGCTTAACTACGAATTCAAAATT
AACAAATTCTGTGGGTGTTA

Downstream 100 bases:

>100_bases
AACACGGACGTTACAGAATTTAGAGGTATATATCATGGCTTATAGCGAAAAAGTAATCGACCATTACGAAAACCCTCGTA
ATGTTGGTTCATTTGAAAAA

Product: cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 419; Mature: 418

Protein sequence:

>419_residues
MSVLNGQTSVFLEMKMKLPIYFDYSATCPVDKRVADKMVQYMTMDGYFGNPASRSHRYGWQAEEAVDTARENIADLLNAD
PREIVFTSGATESDNLAIKGAAHFYNKKGKHIITCKTEHKAVLDPCRQLEREGYEVTYLEPESNGLIDLAKLEAAMRDDT
VLVSIMHVNNEIGVIQDIAAIGELCRSRKIIFHVDAAQSAGKIPLDVQEIKVDLISLSAHKIYGPKGIGALYVRRKPRIR
LEAQMHGGGHERGFRSGTLATHQIVGMGEACRIAKEEMQKDYDHCLAMRDRLLDGIKDMEAVHINGDLDQRVPSNLNISF
EFVEGESLLMALKDLAVSSGSACTSASLEPSYVLRALGLNDELAHSSIRFSFGRYTTAEEIDHAVSQISQAVNKLRDMSP
LWDMYKEGIDLNTVEWAHH

Sequences:

>Translated_419_residues
MSVLNGQTSVFLEMKMKLPIYFDYSATCPVDKRVADKMVQYMTMDGYFGNPASRSHRYGWQAEEAVDTARENIADLLNAD
PREIVFTSGATESDNLAIKGAAHFYNKKGKHIITCKTEHKAVLDPCRQLEREGYEVTYLEPESNGLIDLAKLEAAMRDDT
VLVSIMHVNNEIGVIQDIAAIGELCRSRKIIFHVDAAQSAGKIPLDVQEIKVDLISLSAHKIYGPKGIGALYVRRKPRIR
LEAQMHGGGHERGFRSGTLATHQIVGMGEACRIAKEEMQKDYDHCLAMRDRLLDGIKDMEAVHINGDLDQRVPSNLNISF
EFVEGESLLMALKDLAVSSGSACTSASLEPSYVLRALGLNDELAHSSIRFSFGRYTTAEEIDHAVSQISQAVNKLRDMSP
LWDMYKEGIDLNTVEWAHH
>Mature_418_residues
SVLNGQTSVFLEMKMKLPIYFDYSATCPVDKRVADKMVQYMTMDGYFGNPASRSHRYGWQAEEAVDTARENIADLLNADP
REIVFTSGATESDNLAIKGAAHFYNKKGKHIITCKTEHKAVLDPCRQLEREGYEVTYLEPESNGLIDLAKLEAAMRDDTV
LVSIMHVNNEIGVIQDIAAIGELCRSRKIIFHVDAAQSAGKIPLDVQEIKVDLISLSAHKIYGPKGIGALYVRRKPRIRL
EAQMHGGGHERGFRSGTLATHQIVGMGEACRIAKEEMQKDYDHCLAMRDRLLDGIKDMEAVHINGDLDQRVPSNLNISFE
FVEGESLLMALKDLAVSSGSACTSASLEPSYVLRALGLNDELAHSSIRFSFGRYTTAEEIDHAVSQISQAVNKLRDMSPL
WDMYKEGIDLNTVEWAHH

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=402, Percent_Identity=58.2089552238806, Blast_Score=511, Evalue=1e-145,
Organism=Homo sapiens, GI156713448, Length=418, Percent_Identity=32.7751196172249, Blast_Score=199, Evalue=3e-51,
Organism=Escherichia coli, GI48994898, Length=404, Percent_Identity=76.4851485148515, Blast_Score=673, Evalue=0.0,
Organism=Escherichia coli, GI1789175, Length=399, Percent_Identity=26.3157894736842, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1787970, Length=273, Percent_Identity=29.3040293040293, Blast_Score=95, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI25143064, Length=404, Percent_Identity=59.4059405940594, Blast_Score=504, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI17533177, Length=334, Percent_Identity=33.8323353293413, Blast_Score=171, Evalue=5e-43,
Organism=Saccharomyces cerevisiae, GI6319831, Length=401, Percent_Identity=57.356608478803, Blast_Score=494, Evalue=1e-140,
Organism=Drosophila melanogaster, GI20129463, Length=402, Percent_Identity=57.4626865671642, Blast_Score=490, Evalue=1e-139,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 46691; Mature: 46560

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVLNGQTSVFLEMKMKLPIYFDYSATCPVDKRVADKMVQYMTMDGYFGNPASRSHRYGW
CCCCCCCCEEEEEEEEECEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
QAEEAVDTARENIADLLNADPREIVFTSGATESDNLAIKGAAHFYNKKGKHIITCKTEHK
CHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEEHHHHHCCCCCEEEEEECCCH
AVLDPCRQLEREGYEVTYLEPESNGLIDLAKLEAAMRDDTVLVSIMHVNNEIGVIQDIAA
HHHHHHHHHHHCCCEEEEECCCCCCCEEHHHHHHHHCCCEEEEEEEEECCCCCHHHHHHH
IGELCRSRKIIFHVDAAQSAGKIPLDVQEIKVDLISLSAHKIYGPKGIGALYVRRKPRIR
HHHHHHCCEEEEEEECCCCCCCCCCCHHHHEEEEEEECHHEEECCCCCCEEEEECCCCEE
LEAQMHGGGHERGFRSGTLATHQIVGMGEACRIAKEEMQKDYDHCLAMRDRLLDGIKDME
EEEEECCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCE
AVHINGDLDQRVPSNLNISFEFVEGESLLMALKDLAVSSGSACTSASLEPSYVLRALGLN
EEEECCCHHHCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCC
DELAHSSIRFSFGRYTTAEEIDHAVSQISQAVNKLRDMSPLWDMYKEGIDLNTVEWAHH
HHHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SVLNGQTSVFLEMKMKLPIYFDYSATCPVDKRVADKMVQYMTMDGYFGNPASRSHRYGW
CCCCCCCEEEEEEEEECEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
QAEEAVDTARENIADLLNADPREIVFTSGATESDNLAIKGAAHFYNKKGKHIITCKTEHK
CHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEEHHHHHCCCCCEEEEEECCCH
AVLDPCRQLEREGYEVTYLEPESNGLIDLAKLEAAMRDDTVLVSIMHVNNEIGVIQDIAA
HHHHHHHHHHHCCCEEEEECCCCCCCEEHHHHHHHHCCCEEEEEEEEECCCCCHHHHHHH
IGELCRSRKIIFHVDAAQSAGKIPLDVQEIKVDLISLSAHKIYGPKGIGALYVRRKPRIR
HHHHHHCCEEEEEEECCCCCCCCCCCHHHHEEEEEEECHHEEECCCCCCEEEEECCCCEE
LEAQMHGGGHERGFRSGTLATHQIVGMGEACRIAKEEMQKDYDHCLAMRDRLLDGIKDME
EEEEECCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCE
AVHINGDLDQRVPSNLNISFEFVEGESLLMALKDLAVSSGSACTSASLEPSYVLRALGLN
EEEECCCHHHCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCC
DELAHSSIRFSFGRYTTAEEIDHAVSQISQAVNKLRDMSPLWDMYKEGIDLNTVEWAHH
HHHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA