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The map label for this gene is greA [H]

Identifier: 59711083

GI number: 59711083

Start: 512384

End: 512857

Strand: Direct

Name: greA [H]

Synonym: VF_0476

Alternate gene names: 59711083

Gene position: 512384-512857 (Clockwise)

Preceding gene: 59711080

Following gene: 59711085

Centisome position: 17.68

GC content: 37.97

Gene sequence:

>474_bases
ATGGATAAGGTACCAATGACGGTACGTGGCGAAAAGCTACTGCGTGATGAATTAGAAGTATTATTAAAGCGTCGTCCTTT
GATTTCAAAAGCGATCGGTGAAGCTCGTGAGCTAGGTGATTTAAAAGAAAATGCTGAATATCACGCAGCACGTGAAGAGC
AAGGTATTTGTGAAGCACAGATTCGTGATATTGAATATAAGTTATCTGTAGCTCAGGTTATTGATGTAACTAAAATGACC
AACACTGGTAAAGTTATTTTTGGTACAACAGTGACTTTAATTGATGTGAATACAGACGAAGAAGTGAAATACCAAATCGT
TGCTGATGATGAAGCGAACATTAAAGAAGGTCGAATTTCTGTAAATTCACCGATTGCTCGTGGTTTGATTGGTAAAATGG
AAGGGGATGAAGTAAATATTCAAACTCCTGGCGGTACGAAAGAGTTCGAGATTGACGCTGTAGATTACATTTAA

Upstream 100 bases:

>100_bases
GAAACACTTAAGGTCTACGCTCAATAGTCTGTTTATTGAGAGCCTTTGGTGAGATTTTTATTTGCTGAATTTCTGATTTC
AGCTCTAGAGGTATATAAAT

Downstream 100 bases:

>100_bases
TATTTATTACTTGTATGAAACACAAAGGCCGCATAGTTAGCTCTACTTTAAAGATAACTAATACGGCCTTTTTAATTTCT
TTAGACTAAATGAAAGAAAT

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA [H]

Number of amino acids: Translated: 157; Mature: 157

Protein sequence:

>157_residues
MDKVPMTVRGEKLLRDELEVLLKRRPLISKAIGEARELGDLKENAEYHAAREEQGICEAQIRDIEYKLSVAQVIDVTKMT
NTGKVIFGTTVTLIDVNTDEEVKYQIVADDEANIKEGRISVNSPIARGLIGKMEGDEVNIQTPGGTKEFEIDAVDYI

Sequences:

>Translated_157_residues
MDKVPMTVRGEKLLRDELEVLLKRRPLISKAIGEARELGDLKENAEYHAAREEQGICEAQIRDIEYKLSVAQVIDVTKMT
NTGKVIFGTTVTLIDVNTDEEVKYQIVADDEANIKEGRISVNSPIARGLIGKMEGDEVNIQTPGGTKEFEIDAVDYI
>Mature_157_residues
MDKVPMTVRGEKLLRDELEVLLKRRPLISKAIGEARELGDLKENAEYHAAREEQGICEAQIRDIEYKLSVAQVIDVTKMT
NTGKVIFGTTVTLIDVNTDEEVKYQIVADDEANIKEGRISVNSPIARGLIGKMEGDEVNIQTPGGTKEFEIDAVDYI

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082225, Length=158, Percent_Identity=64.5569620253165, Blast_Score=199, Evalue=9e-53,
Organism=Escherichia coli, GI87082261, Length=159, Percent_Identity=30.8176100628931, Blast_Score=75, Evalue=3e-15,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 17491; Mature: 17491

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: PS00829 GREAB_1 ; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKVPMTVRGEKLLRDELEVLLKRRPLISKAIGEARELGDLKENAEYHAAREEQGICEAQ
CCCCCCEECCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHCCHHHHCCCCCCCHHHH
IRDIEYKLSVAQVIDVTKMTNTGKVIFGTTVTLIDVNTDEEVKYQIVADDEANIKEGRIS
HHCCCEEEHHHHHHHHHCCCCCCCEEEEEEEEEEECCCCCCEEEEEEECCCCCCCCCEEE
VNSPIARGLIGKMEGDEVNIQTPGGTKEFEIDAVDYI
ECCCHHHHHHCCCCCCEEEEECCCCCCEEEEEEECCC
>Mature Secondary Structure
MDKVPMTVRGEKLLRDELEVLLKRRPLISKAIGEARELGDLKENAEYHAAREEQGICEAQ
CCCCCCEECCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHCCHHHHCCCCCCCHHHH
IRDIEYKLSVAQVIDVTKMTNTGKVIFGTTVTLIDVNTDEEVKYQIVADDEANIKEGRIS
HHCCCEEEHHHHHHHHHCCCCCCCEEEEEEEEEEECCCCCCEEEEEEECCCCCCCCCEEE
VNSPIARGLIGKMEGDEVNIQTPGGTKEFEIDAVDYI
ECCCHHHHHHCCCCCCEEEEECCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]