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The map label for this gene is rrmJ [H]

Identifier: 59711085

GI number: 59711085

Start: 513372

End: 514001

Strand: Direct

Name: rrmJ [H]

Synonym: VF_0478

Alternate gene names: 59711085

Gene position: 513372-514001 (Clockwise)

Preceding gene: 59711083

Following gene: 59711086

Centisome position: 17.72

GC content: 40.0

Gene sequence:

>630_bases
ATGAGTAAGAATAAAGTTTCAGCGAGCTCTGGTCGCTGGTTAAAAGAACATTTTGATGATAAATACGTTCTTGAGGCTCA
AAAAAGGGGTTATCGCTCTCGTGCTATTTTTAAAATTGAAGAAATTCAAAATAAAGACAAGCTTTTAAAACCAGGAATGA
CAGTCGTTGACCTTGGTGCTGCACCTGGTGGCTGGTCACAATATGCGGTTGAGCAAGTTGGTGATGAAGGACAAGTTATT
GCTTGTGATATCTTGCCAATGGACTCTATTGCTGGTGTAAGTTTTTTACAGGGGGACTTTAGGGAAGAAGCAGTATTGGA
TGCATTATTAGAGAGAATTCAACCTGATATGGTTGATGTTGTAATGTCTGATATGGCGCCAAATATGAGTGGTAACTTAG
CGGTAGATCAGCCTCGTGCGATGTACTTAGTTGAGTTAGCTTTGGATATGTGTCGTCAAGTTCTTGCGCCAAATGGCAGC
TTTACTGTGAAAGTATTCCAAGGTGAAGGCTTTGATCAGTACTTGCAAGAAGTGCGTAATATGTTCAAAGTAGTAAAAAT
CAGAAAGCCTGATTCATCACGTGCTCGTTCACGTGAAGTATATATTGTAGCGACTGGTTACAAAGGATAA

Upstream 100 bases:

>100_bases
ATATTAAGGGTTGAAAACAGCCTATTCTAACGCCATCTAAGCAGGAAGACTATTTTTTGTTGCAGTCTTCTAATTTTAAT
TAAGTATTTTTAGGTAATGC

Downstream 100 bases:

>100_bases
GTTTTACGTAGTAGATACAGAATATAAACTGTAGTACTCTGTCTTTCATCTATTTAGTTAACGCGGGGTTAACACCTTGA
GTGACATGGCAAAAAATCTA

Product: 23S rRNA methyltransferase J

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase [H]

Number of amino acids: Translated: 209; Mature: 208

Protein sequence:

>209_residues
MSKNKVSASSGRWLKEHFDDKYVLEAQKRGYRSRAIFKIEEIQNKDKLLKPGMTVVDLGAAPGGWSQYAVEQVGDEGQVI
ACDILPMDSIAGVSFLQGDFREEAVLDALLERIQPDMVDVVMSDMAPNMSGNLAVDQPRAMYLVELALDMCRQVLAPNGS
FTVKVFQGEGFDQYLQEVRNMFKVVKIRKPDSSRARSREVYIVATGYKG

Sequences:

>Translated_209_residues
MSKNKVSASSGRWLKEHFDDKYVLEAQKRGYRSRAIFKIEEIQNKDKLLKPGMTVVDLGAAPGGWSQYAVEQVGDEGQVI
ACDILPMDSIAGVSFLQGDFREEAVLDALLERIQPDMVDVVMSDMAPNMSGNLAVDQPRAMYLVELALDMCRQVLAPNGS
FTVKVFQGEGFDQYLQEVRNMFKVVKIRKPDSSRARSREVYIVATGYKG
>Mature_208_residues
SKNKVSASSGRWLKEHFDDKYVLEAQKRGYRSRAIFKIEEIQNKDKLLKPGMTVVDLGAAPGGWSQYAVEQVGDEGQVIA
CDILPMDSIAGVSFLQGDFREEAVLDALLERIQPDMVDVVMSDMAPNMSGNLAVDQPRAMYLVELALDMCRQVLAPNGSF
TVKVFQGEGFDQYLQEVRNMFKVVKIRKPDSSRARSREVYIVATGYKG

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit [H]

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family [H]

Homologues:

Organism=Homo sapiens, GI7019377, Length=217, Percent_Identity=34.5622119815668, Blast_Score=134, Evalue=6e-32,
Organism=Homo sapiens, GI194097365, Length=188, Percent_Identity=32.4468085106383, Blast_Score=112, Evalue=2e-25,
Organism=Homo sapiens, GI29029591, Length=199, Percent_Identity=32.6633165829146, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI29029589, Length=199, Percent_Identity=32.6633165829146, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI7110661, Length=199, Percent_Identity=32.6633165829146, Blast_Score=106, Evalue=1e-23,
Organism=Escherichia coli, GI1789569, Length=208, Percent_Identity=74.0384615384615, Blast_Score=322, Evalue=1e-89,
Organism=Caenorhabditis elegans, GI17553474, Length=220, Percent_Identity=32.2727272727273, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71987550, Length=189, Percent_Identity=32.2751322751323, Blast_Score=106, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI71987561, Length=189, Percent_Identity=32.2751322751323, Blast_Score=105, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17553860, Length=189, Percent_Identity=32.2751322751323, Blast_Score=105, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17554650, Length=191, Percent_Identity=30.8900523560209, Blast_Score=101, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6319535, Length=198, Percent_Identity=32.3232323232323, Blast_Score=106, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6319796, Length=210, Percent_Identity=30.952380952381, Blast_Score=101, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6321302, Length=96, Percent_Identity=37.5, Blast_Score=72, Evalue=6e-14,
Organism=Drosophila melanogaster, GI21356387, Length=212, Percent_Identity=34.4339622641509, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI18859957, Length=188, Percent_Identity=35.1063829787234, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24647580, Length=198, Percent_Identity=30.3030303030303, Blast_Score=91, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24648639, Length=202, Percent_Identity=30.6930693069307, Blast_Score=91, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015507
- InterPro:   IPR002877 [H]

Pfam domain/function: PF01728 FtsJ [H]

EC number: =2.1.1.166 [H]

Molecular weight: Translated: 23363; Mature: 23232

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKNKVSASSGRWLKEHFDDKYVLEAQKRGYRSRAIFKIEEIQNKDKLLKPGMTVVDLGA
CCCCCCCCCCCCHHHHHCCCCEEHHHHHCCCCHHHEEEHHHHCCHHHHHCCCCEEEECCC
APGGWSQYAVEQVGDEGQVIACDILPMDSIAGVSFLQGDFREEAVLDALLERIQPDMVDV
CCCCHHHHHHHHCCCCCCEEEEEECCCCHHCCHHHHHHHHHHHHHHHHHHHHHCHHHHHH
VMSDMAPNMSGNLAVDQPRAMYLVELALDMCRQVLAPNGSFTVKVFQGEGFDQYLQEVRN
HHHHCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHH
MFKVVKIRKPDSSRARSREVYIVATGYKG
HHHHHEECCCCCCCCCCCEEEEEEECCCC
>Mature Secondary Structure 
SKNKVSASSGRWLKEHFDDKYVLEAQKRGYRSRAIFKIEEIQNKDKLLKPGMTVVDLGA
CCCCCCCCCCCHHHHHCCCCEEHHHHHCCCCHHHEEEHHHHCCHHHHHCCCCEEEECCC
APGGWSQYAVEQVGDEGQVIACDILPMDSIAGVSFLQGDFREEAVLDALLERIQPDMVDV
CCCCHHHHHHHHCCCCCCEEEEEECCCCHHCCHHHHHHHHHHHHHHHHHHHHHCHHHHHH
VMSDMAPNMSGNLAVDQPRAMYLVELALDMCRQVLAPNGSFTVKVFQGEGFDQYLQEVRN
HHHHCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHH
MFKVVKIRKPDSSRARSREVYIVATGYKG
HHHHHEECCCCCCCCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA