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The map label for this gene is thyA [H]

Identifier: 59711067

GI number: 59711067

Start: 491451

End: 492308

Strand: Direct

Name: thyA [H]

Synonym: VF_0460

Alternate gene names: 59711067

Gene position: 491451-492308 (Clockwise)

Preceding gene: 59711066

Following gene: 59711069

Centisome position: 16.96

GC content: 38.69

Gene sequence:

>858_bases
ATGACCGTGAAGCAGTATTTAGATTTATGCCAACGCATTGTAGATGAAGGAACTTGGATCGAAAACGAGCGCACAGGAAA
GCGTTGTTTAACGGTGATTAATGCGGATTTAACTTATGATGTAGAAAATGGTGTTTTCCCTCTTGTGACAACGCGTAAAA
GCTTTTGGAAAGCGGCAGTAGCTGAAATGATTGGTTATATTCGTGGTTACGATAGCGCAGAAGACTTCCGTAAAATTGGA
ACAAAAACATGGGATGCGAATGCTAACCTTAACGATGCATGGCTAAACAACCCTCATCGTAAAGGTGATGATGACATGGG
CCGAGTATATGGTGTGCAAGGTCGTTCATGGGCAAAACCAAATGGTGAGTTTGTCGATCAACTGAAAAAAATCATTGATG
ATTTAAGTCAGGGAATTGATGATCGTGGTGAGATTTTAAATTTTTATAACCCAGGTGAGTTTGATATGGGATGTTTGCGT
CCGTGTATGTACTCACACCATTTTTCATTACTTGGTGATACGTTATATTTAAACAGTACGCAACGTTCATGTGATGTTCC
GCTAGGGCTTAATTTCAATATGGTTCAAGTGTATTTCTTATTAGCGATAGTGGCACAAATTACAGGGCATAAAGCAGGTA
AGGCGTACCATAAGATTGTAAATGCACATATTTATGAAGACCAACTTGAACTAATGCAAAGTGTGCAGCTAAAGCGTGAA
CCGTTACAAGCCCCAATCTTTAAAATAAACCCTGAAATTAAGTCACTAGAAGATTTAGAAACATGGGTAACTATGGATGA
CTTTGAAGTGATTGGCTATGAAAGCCACGAAGCAATTAAGTATCCTTTTTCTGTTTAA

Upstream 100 bases:

>100_bases
ACCACAAAGTACGACAAAGAAAAAATAAGTTAGAATAGTTTTATTATATAGTGGCCTCTGAACTCATGAGGCCATTTTTT
TAAGTTAAATTTTGAGAGAA

Downstream 100 bases:

>100_bases
TATTATTTATTAGATATAATAAAAAAGCCCCGAAGCATAACTTCGGGGCTTTTTTTATTCGTGTTTTTTAGTTTTAAACT
GTTAGTGCTAATACACTACC

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase [H]

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MTVKQYLDLCQRIVDEGTWIENERTGKRCLTVINADLTYDVENGVFPLVTTRKSFWKAAVAEMIGYIRGYDSAEDFRKIG
TKTWDANANLNDAWLNNPHRKGDDDMGRVYGVQGRSWAKPNGEFVDQLKKIIDDLSQGIDDRGEILNFYNPGEFDMGCLR
PCMYSHHFSLLGDTLYLNSTQRSCDVPLGLNFNMVQVYFLLAIVAQITGHKAGKAYHKIVNAHIYEDQLELMQSVQLKRE
PLQAPIFKINPEIKSLEDLETWVTMDDFEVIGYESHEAIKYPFSV

Sequences:

>Translated_285_residues
MTVKQYLDLCQRIVDEGTWIENERTGKRCLTVINADLTYDVENGVFPLVTTRKSFWKAAVAEMIGYIRGYDSAEDFRKIG
TKTWDANANLNDAWLNNPHRKGDDDMGRVYGVQGRSWAKPNGEFVDQLKKIIDDLSQGIDDRGEILNFYNPGEFDMGCLR
PCMYSHHFSLLGDTLYLNSTQRSCDVPLGLNFNMVQVYFLLAIVAQITGHKAGKAYHKIVNAHIYEDQLELMQSVQLKRE
PLQAPIFKINPEIKSLEDLETWVTMDDFEVIGYESHEAIKYPFSV
>Mature_284_residues
TVKQYLDLCQRIVDEGTWIENERTGKRCLTVINADLTYDVENGVFPLVTTRKSFWKAAVAEMIGYIRGYDSAEDFRKIGT
KTWDANANLNDAWLNNPHRKGDDDMGRVYGVQGRSWAKPNGEFVDQLKKIIDDLSQGIDDRGEILNFYNPGEFDMGCLRP
CMYSHHFSLLGDTLYLNSTQRSCDVPLGLNFNMVQVYFLLAIVAQITGHKAGKAYHKIVNAHIYEDQLELMQSVQLKREP
LQAPIFKINPEIKSLEDLETWVTMDDFEVIGYESHEAIKYPFSV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis [H]

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507751, Length=289, Percent_Identity=31.8339100346021, Blast_Score=150, Evalue=1e-36,
Organism=Escherichia coli, GI1789191, Length=283, Percent_Identity=31.095406360424, Blast_Score=147, Evalue=7e-37,
Organism=Caenorhabditis elegans, GI71993377, Length=292, Percent_Identity=27.7397260273973, Blast_Score=129, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6324648, Length=303, Percent_Identity=28.3828382838284, Blast_Score=101, Evalue=1e-22,
Organism=Drosophila melanogaster, GI17137556, Length=291, Percent_Identity=27.4914089347079, Blast_Score=122, Evalue=3e-28,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000398
- InterPro:   IPR020940 [H]

Pfam domain/function: PF00303 Thymidylat_synt [H]

EC number: =2.1.1.45 [H]

Molecular weight: Translated: 32664; Mature: 32533

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVKQYLDLCQRIVDEGTWIENERTGKRCLTVINADLTYDVENGVFPLVTTRKSFWKAAV
CCHHHHHHHHHHHHCCCCCCCCCCCHHHHEEEEECCEEEECCCCEEEEEECHHHHHHHHH
AEMIGYIRGYDSAEDFRKIGTKTWDANANLNDAWLNNPHRKGDDDMGRVYGVQGRSWAKP
HHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCHHHEEECCCCCCCCC
NGEFVDQLKKIIDDLSQGIDDRGEILNFYNPGEFDMGCLRPCMYSHHFSLLGDTLYLNST
CHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHCCEEEECCC
QRSCDVPLGLNFNMVQVYFLLAIVAQITGHKAGKAYHKIVNAHIYEDQLELMQSVQLKRE
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PLQAPIFKINPEIKSLEDLETWVTMDDFEVIGYESHEAIKYPFSV
CCCCCCEEECCCHHHHHHHHHHHCCCCEEEECCCCCCCEECCCCC
>Mature Secondary Structure 
TVKQYLDLCQRIVDEGTWIENERTGKRCLTVINADLTYDVENGVFPLVTTRKSFWKAAV
CHHHHHHHHHHHHCCCCCCCCCCCHHHHEEEEECCEEEECCCCEEEEEECHHHHHHHHH
AEMIGYIRGYDSAEDFRKIGTKTWDANANLNDAWLNNPHRKGDDDMGRVYGVQGRSWAKP
HHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCHHHEEECCCCCCCCC
NGEFVDQLKKIIDDLSQGIDDRGEILNFYNPGEFDMGCLRPCMYSHHFSLLGDTLYLNST
CHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHCCEEEECCC
QRSCDVPLGLNFNMVQVYFLLAIVAQITGHKAGKAYHKIVNAHIYEDQLELMQSVQLKRE
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PLQAPIFKINPEIKSLEDLETWVTMDDFEVIGYESHEAIKYPFSV
CCCCCCEEECCCHHHHHHHHHHHCCCCEEEECCCCCCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA