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The map label for this gene is nudH

Identifier: 59711063

GI number: 59711063

Start: 486918

End: 487430

Strand: Direct

Name: nudH

Synonym: VF_0456

Alternate gene names: 59711063

Gene position: 486918-487430 (Clockwise)

Preceding gene: 59711061

Following gene: 59711064

Centisome position: 16.8

GC content: 42.3

Gene sequence:

>513_bases
GTGATAGATGGCGATGGTTTCCGCCCGAACGTCGGGATAGTGATATGTAATAGCCATGGTCAAGTATTCTGGGCGAAGCG
ATACGGACAGCATTCGTGGCAATTTCCGCAAGGTGGTATTGATGATGGTGAGACTCCAGAACAAGCCATGTACCGTGAAT
TATACGAAGAGGTAGGTCTGACAAAAAATGATGTTCGTATTTTAGCAAGCAGCCGTCATTGGCTACGATATAAATTACCG
AAACGTCTAGTTAGATGGGACTCTAAACCTGTTTGTATCGGACAAAAACAAAAATGGTTTTTGTTACGCTTAGAATGTGA
TGAATCCAAGGTTAATATGCAAAGAGATCGCTCGCCTGAATTTGATGGTTGGCGATGGGTAAGCTACTGGTACCCTGTAA
GACAAGTGGTTTCATTTAAGCGTGATGTTTATCGTCGAGCACTAAAAGAGTTCGCGGTGATCGCTATGCCGTTTAAGGAA
CGAAAATTTAAAAGGAAAGGTAAAAAAGGTTAA

Upstream 100 bases:

>100_bases
TATTTTGGTAAAACGTGACAAGAAAGCGTGATCCTGTTTGCTCAAAGAGCCGATCTGTGAAAAAATCAGAGTCAATATAA
GTTTATGATGAGGTCATCCA

Downstream 100 bases:

>100_bases
GCGGTCGATACCTTATATAACCTTAAGGATTGGCAAGCATGCTTACTCAACTCAGGGATATAGTTGATCACGTAGCTAAA
GCGGAGTCTCTTGATGATGC

Product: dinucleoside polyphosphate hydrolase

Products: GTP; GMP

Alternate protein names: (Di)nucleoside polyphosphate hydrolase

Number of amino acids: Translated: 170; Mature: 170

Protein sequence:

>170_residues
MIDGDGFRPNVGIVICNSHGQVFWAKRYGQHSWQFPQGGIDDGETPEQAMYRELYEEVGLTKNDVRILASSRHWLRYKLP
KRLVRWDSKPVCIGQKQKWFLLRLECDESKVNMQRDRSPEFDGWRWVSYWYPVRQVVSFKRDVYRRALKEFAVIAMPFKE
RKFKRKGKKG

Sequences:

>Translated_170_residues
MIDGDGFRPNVGIVICNSHGQVFWAKRYGQHSWQFPQGGIDDGETPEQAMYRELYEEVGLTKNDVRILASSRHWLRYKLP
KRLVRWDSKPVCIGQKQKWFLLRLECDESKVNMQRDRSPEFDGWRWVSYWYPVRQVVSFKRDVYRRALKEFAVIAMPFKE
RKFKRKGKKG
>Mature_170_residues
MIDGDGFRPNVGIVICNSHGQVFWAKRYGQHSWQFPQGGIDDGETPEQAMYRELYEEVGLTKNDVRILASSRHWLRYKLP
KRLVRWDSKPVCIGQKQKWFLLRLECDESKVNMQRDRSPEFDGWRWVSYWYPVRQVVSFKRDVYRRALKEFAVIAMPFKE
RKFKRKGKKG

Specific function: Accelerates the degradation of transcripts by removing pyrophosphate from the 5'-end of triphosphorylated RNA, leading to a more labile monophosphorylated state that can stimulate subsequent ribonuclease cleavage

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain

Homologues:

Organism=Escherichia coli, GI1789194, Length=164, Percent_Identity=75, Blast_Score=270, Evalue=4e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RPPH_VIBF1 (Q5E7P5)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_203839.1
- ProteinModelPortal:   Q5E7P5
- SMR:   Q5E7P5
- STRING:   Q5E7P5
- GeneID:   3278152
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_0456
- NMPDR:   fig|312309.3.peg.456
- eggNOG:   COG0494
- HOGENOM:   HBG302451
- OMA:   GQKQIWY
- PhylomeDB:   Q5E7P5
- ProtClustDB:   PRK00714
- BioCyc:   VFIS312309:VF0456-MONOMER
- HAMAP:   MF_00298
- InterPro:   IPR020476
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797
- InterPro:   IPR022927
- Gene3D:   G3DSA:3.90.79.10
- PRINTS:   PR00502

Pfam domain/function: PF00293 NUDIX; SSF55811 NUDIX_hydrolase

EC number: 3.6.1.17

Molecular weight: Translated: 20377; Mature: 20377

Theoretical pI: Translated: 10.46; Mature: 10.46

Prosite motif: PS51462 NUDIX; PS00893 NUDIX_BOX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDGDGFRPNVGIVICNSHGQVFWAKRYGQHSWQFPQGGIDDGETPEQAMYRELYEEVGL
CCCCCCCCCCCCEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCC
TKNDVRILASSRHWLRYKLPKRLVRWDSKPVCIGQKQKWFLLRLECDESKVNMQRDRSPE
CCCCEEEEECCCCCEEHHHHHHHHHCCCCCEEECCCCCEEEEEEECCCCCCCCCCCCCCC
FDGWRWVSYWYPVRQVVSFKRDVYRRALKEFAVIAMPFKERKFKRKGKKG
CCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHCCCCC
>Mature Secondary Structure
MIDGDGFRPNVGIVICNSHGQVFWAKRYGQHSWQFPQGGIDDGETPEQAMYRELYEEVGL
CCCCCCCCCCCCEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCC
TKNDVRILASSRHWLRYKLPKRLVRWDSKPVCIGQKQKWFLLRLECDESKVNMQRDRSPE
CCCCEEEEECCCCCEEHHHHHHHHHCCCCCEEECCCCCEEEEEEECCCCCCCCCCCCCCC
FDGWRWVSYWYPVRQVVSFKRDVYRRALKEFAVIAMPFKERKFKRKGKKG
CCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: P1,P4-bis(5'-guanosyl) tetraphosphate; H2O

Specific reaction: P1,P4-bis(5'-guanosyl) tetraphosphate + H2O = GTP + GMP

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA