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The map label for this gene is ptsP [H]

Identifier: 59711064

GI number: 59711064

Start: 487469

End: 489730

Strand: Direct

Name: ptsP [H]

Synonym: VF_0457

Alternate gene names: 59711064

Gene position: 487469-489730 (Clockwise)

Preceding gene: 59711063

Following gene: 59711065

Centisome position: 16.82

GC content: 38.55

Gene sequence:

>2262_bases
ATGCTTACTCAACTCAGGGATATAGTTGATCACGTAGCTAAAGCGGAGTCTCTTGATGATGCTTTAGCGTTGCTCGTGAA
AAATACTCGTCACTCTATGCATACAGAGTGCTGCTCAGTCTATTTGATGAGTAATGATAACCTGCGCTTAGAATTAATGG
CGACAGAAGGACTAAAAGCGAAACCAAAAACCGTTTCATTGGGGATTGATGAAGGTTTAGTTGGATTGGTTGCGCGTAGT
TGTGAACCGTTAAACTTAGCGAATGCTTCTGTACACCCTTCATATAAATACATTCCTAGTATTGCTGAATATAAGTTTAA
CTCCTTCCTAGCAACCCCGATTATTTACCGACGCCAAAACCTTGGTGTGCTTGTTGTACAACAAAAGGAAAAACGTCAGT
TTACTGAAGTTGAAGAATCCTTTTTAGTCACCTTATCCGCGCAACTTGCAGTTATACTTGCTCATGCTAAAGCTCATGAA
AGTTGGTCAACTCAATTACAACCCCAATCGATTGAACAAAAGCAATTCAAAGGTATCTCTGCATCAAATGGTGTGGCTAT
TGCTCCATTATGGTTTGACGATGCTCAACCAAGAATAGAATCCATTCTACCAAGTTCATGTCTTGATACAGAGCAAGAAC
AAGATCGTTTAGGTACTGCGGTTGAGTTAGCTTTAAAAGATTTCCGCCGAGCTAAAAAACGCTTTGAGCAAGAATTAAAT
ACCGAAACGTTGGCTATTTTTGATCTTTTCACTCACTTATTAAATGATCCTATGTTGAAAGGAGATCTGAAAAAACAGAT
CAACCAAGGTGATTCCGCCGAGTGGGCATTGCGTCAAGTTATTGAGTCATACGCCGGGCGTTTTGAAAAAATGAGTGATT
CATACCTCAGTGAGCGAGCGAATGATGTTCGTGAGCTAGGACAGAGATTACTTTATTTTTTAAATTATGAAGGTAAGTTA
GAGCTGGATTTAGCTCATCCCGTGATTTTAGTGACGACTCAGTTAACGGCTTCTATGTTAGCGAGTGTTCCTCAAGATAA
ATTAAAAGCCGTTATTTCGCTTGAAGGTGCGGCAAACTCTCACGCAGCCATTTTATCAAGAGCGCTTGGTATTCCTGCAA
TTATGGGGGTGGAGTTTAATCCAACTCATTACCATCAAAACCTAGCTATTGTAGATGGCTATAGTGGTGCTCTATACATC
AAGCCAACAGAAGCGCTAATTCAAGAATATACAGAGCTACAAAATGAAGAGTTAGCCCTTTCTGAATTAATCACTCAAGA
TCTTGATAAACCTACATTTACACGTGATGAGCATCAAATGAAAATTTATTTGAATGCAGGGTTAAGTGCAGATACGAGTA
TTGCTGTTAATCAGGGAGTTGATGGTGTGGGTCTATATCGAACTGAAATTCCTTTTTTATTACAACAGAGATTTCCATCA
GAAGAAGAGCAGTACCTACAATACAAAACTATTTTAGAAACGTACCCTAAAAAGCCAGTCATTATGAGGACGTTAGATAT
TGGTGGTGATAAACCATTACCGTATTTACCAATTGAAGAAGAGAATCCATTTTTAGGTTGGCGTGGTATTCGTTTTACTT
TAGATCATCCTGATATCTTTCTTCTGCAACTAAAAGCGATGGTTAGAGCAAATATAGGTAATGGTAATTTGTCCATTATG
TTACCTATGATTTCAGGCTTACCTGAATTAAAGCAAGCAAGAAAGCTTATTAACCAAGCCTATGAAGAAGTGCTTGGTGA
GTCGGAAGGAGACTTTTCACAACCTCGTATAGGTATGATGATAGAAGTGCCTTCTATGCTGTATTTGTTACCAAGAGTTG
TTGGATTAGTGGATTTTATTTCTGTAGGTACTAATGACTTAACTCAATACCTTTTAGCGGTGGATCGAAATAACGCACGA
GTTTCACATGTTTATGAATCTTATCATCCCGCAGTGTTACTCGCATTAAAGCAAATTATTGATACGGCAAATCAATATAA
ATTGCCTGTTAGTGTTTGTGGAGAACTTGCGGGGGACCCAATTGGTTGTTTATTACTGCTTGGTTTGGGTTATCAAAGCT
TAAGTATGAACACCTCTAACGTAGCCAAAGTAAAATATATTATTAGGCAAGTATCGCAGCAAGAAGTTGAACAATTAGTT
GCGACTCTTTTACATCACGATGATGCAGAGCAAATTTTAACTGATATGATTGGCTTCTTTGAAGAGAAAGATCTCTCTGG
GTTTATTCGAGCAGGCAGGTAA

Upstream 100 bases:

>100_bases
TCGCGGTGATCGCTATGCCGTTTAAGGAACGAAAATTTAAAAGGAAAGGTAAAAAAGGTTAAGCGGTCGATACCTTATAT
AACCTTAAGGATTGGCAAGC

Downstream 100 bases:

>100_bases
TGACAGTGGATTTTAGTTCGCTTTTTGTTTTATATCTTTTGCTAGGTAGTATCGTTGGCGTAATGGCTGGATTACTTGGA
ATCGGTGGTGGTTTGTTAGT

Product: fused phosphoenolpyruvate-protein phosphotransferase PtsP/GAF domain

Products: NA

Alternate protein names: Enzyme I-Ntr; Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 753; Mature: 753

Protein sequence:

>753_residues
MLTQLRDIVDHVAKAESLDDALALLVKNTRHSMHTECCSVYLMSNDNLRLELMATEGLKAKPKTVSLGIDEGLVGLVARS
CEPLNLANASVHPSYKYIPSIAEYKFNSFLATPIIYRRQNLGVLVVQQKEKRQFTEVEESFLVTLSAQLAVILAHAKAHE
SWSTQLQPQSIEQKQFKGISASNGVAIAPLWFDDAQPRIESILPSSCLDTEQEQDRLGTAVELALKDFRRAKKRFEQELN
TETLAIFDLFTHLLNDPMLKGDLKKQINQGDSAEWALRQVIESYAGRFEKMSDSYLSERANDVRELGQRLLYFLNYEGKL
ELDLAHPVILVTTQLTASMLASVPQDKLKAVISLEGAANSHAAILSRALGIPAIMGVEFNPTHYHQNLAIVDGYSGALYI
KPTEALIQEYTELQNEELALSELITQDLDKPTFTRDEHQMKIYLNAGLSADTSIAVNQGVDGVGLYRTEIPFLLQQRFPS
EEEQYLQYKTILETYPKKPVIMRTLDIGGDKPLPYLPIEEENPFLGWRGIRFTLDHPDIFLLQLKAMVRANIGNGNLSIM
LPMISGLPELKQARKLINQAYEEVLGESEGDFSQPRIGMMIEVPSMLYLLPRVVGLVDFISVGTNDLTQYLLAVDRNNAR
VSHVYESYHPAVLLALKQIIDTANQYKLPVSVCGELAGDPIGCLLLLGLGYQSLSMNTSNVAKVKYIIRQVSQQEVEQLV
ATLLHHDDAEQILTDMIGFFEEKDLSGFIRAGR

Sequences:

>Translated_753_residues
MLTQLRDIVDHVAKAESLDDALALLVKNTRHSMHTECCSVYLMSNDNLRLELMATEGLKAKPKTVSLGIDEGLVGLVARS
CEPLNLANASVHPSYKYIPSIAEYKFNSFLATPIIYRRQNLGVLVVQQKEKRQFTEVEESFLVTLSAQLAVILAHAKAHE
SWSTQLQPQSIEQKQFKGISASNGVAIAPLWFDDAQPRIESILPSSCLDTEQEQDRLGTAVELALKDFRRAKKRFEQELN
TETLAIFDLFTHLLNDPMLKGDLKKQINQGDSAEWALRQVIESYAGRFEKMSDSYLSERANDVRELGQRLLYFLNYEGKL
ELDLAHPVILVTTQLTASMLASVPQDKLKAVISLEGAANSHAAILSRALGIPAIMGVEFNPTHYHQNLAIVDGYSGALYI
KPTEALIQEYTELQNEELALSELITQDLDKPTFTRDEHQMKIYLNAGLSADTSIAVNQGVDGVGLYRTEIPFLLQQRFPS
EEEQYLQYKTILETYPKKPVIMRTLDIGGDKPLPYLPIEEENPFLGWRGIRFTLDHPDIFLLQLKAMVRANIGNGNLSIM
LPMISGLPELKQARKLINQAYEEVLGESEGDFSQPRIGMMIEVPSMLYLLPRVVGLVDFISVGTNDLTQYLLAVDRNNAR
VSHVYESYHPAVLLALKQIIDTANQYKLPVSVCGELAGDPIGCLLLLGLGYQSLSMNTSNVAKVKYIIRQVSQQEVEQLV
ATLLHHDDAEQILTDMIGFFEEKDLSGFIRAGR
>Mature_753_residues
MLTQLRDIVDHVAKAESLDDALALLVKNTRHSMHTECCSVYLMSNDNLRLELMATEGLKAKPKTVSLGIDEGLVGLVARS
CEPLNLANASVHPSYKYIPSIAEYKFNSFLATPIIYRRQNLGVLVVQQKEKRQFTEVEESFLVTLSAQLAVILAHAKAHE
SWSTQLQPQSIEQKQFKGISASNGVAIAPLWFDDAQPRIESILPSSCLDTEQEQDRLGTAVELALKDFRRAKKRFEQELN
TETLAIFDLFTHLLNDPMLKGDLKKQINQGDSAEWALRQVIESYAGRFEKMSDSYLSERANDVRELGQRLLYFLNYEGKL
ELDLAHPVILVTTQLTASMLASVPQDKLKAVISLEGAANSHAAILSRALGIPAIMGVEFNPTHYHQNLAIVDGYSGALYI
KPTEALIQEYTELQNEELALSELITQDLDKPTFTRDEHQMKIYLNAGLSADTSIAVNQGVDGVGLYRTEIPFLLQQRFPS
EEEQYLQYKTILETYPKKPVIMRTLDIGGDKPLPYLPIEEENPFLGWRGIRFTLDHPDIFLLQLKAMVRANIGNGNLSIM
LPMISGLPELKQARKLINQAYEEVLGESEGDFSQPRIGMMIEVPSMLYLLPRVVGLVDFISVGTNDLTQYLLAVDRNNAR
VSHVYESYHPAVLLALKQIIDTANQYKLPVSVCGELAGDPIGCLLLLGLGYQSLSMNTSNVAKVKYIIRQVSQQEVEQLV
ATLLHHDDAEQILTDMIGFFEEKDLSGFIRAGR

Specific function: Component of the phosphoenolpyruvate-dependent nitrogen- metabolic phosphotransferase system (nitrogen-metabolic PTS), that seems to be involved in regulating nitrogen metabolism. Enzyme I- Ntr transfers the phosphoryl group from phosphoenolpyruvate (PEP)

COG id: COG3605

COG function: function code T; Signal transduction protein containing GAF and PtsI domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GAF domain [H]

Homologues:

Organism=Escherichia coli, GI1789193, Length=754, Percent_Identity=49.6021220159151, Blast_Score=729, Evalue=0.0,
Organism=Escherichia coli, GI1788756, Length=479, Percent_Identity=37.9958246346555, Blast_Score=316, Evalue=4e-87,
Organism=Escherichia coli, GI1788726, Length=550, Percent_Identity=32.7272727272727, Blast_Score=263, Evalue=3e-71,
Organism=Escherichia coli, GI48994992, Length=561, Percent_Identity=31.729055258467, Blast_Score=260, Evalue=3e-70,
Organism=Escherichia coli, GI1787994, Length=419, Percent_Identity=26.491646778043, Blast_Score=97, Evalue=4e-21,
Organism=Escherichia coli, GI226510935, Length=177, Percent_Identity=28.2485875706215, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003018
- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF01590 GAF; PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 84293; Mature: 84293

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTQLRDIVDHVAKAESLDDALALLVKNTRHSMHTECCSVYLMSNDNLRLELMATEGLKA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCEEEEEEECCCCCC
KPKTVSLGIDEGLVGLVARSCEPLNLANASVHPSYKYIPSIAEYKFNSFLATPIIYRRQN
CCCEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHCHHHEEECC
LGVLVVQQKEKRQFTEVEESFLVTLSAQLAVILAHAKAHESWSTQLQPQSIEQKQFKGIS
CCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCC
ASNGVAIAPLWFDDAQPRIESILPSSCLDTEQEQDRLGTAVELALKDFRRAKKRFEQELN
CCCCEEEEEEECCCCCHHHHHHCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TETLAIFDLFTHLLNDPMLKGDLKKQINQGDSAEWALRQVIESYAGRFEKMSDSYLSERA
CHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NDVRELGQRLLYFLNYEGKLELDLAHPVILVTTQLTASMLASVPQDKLKAVISLEGAANS
HHHHHHHHHHHHHCCCCCEEEEEECCCEEEEEHHHHHHHHHHCCHHHHHEEEEECCCCCC
HAAILSRALGIPAIMGVEFNPTHYHQNLAIVDGYSGALYIKPTEALIQEYTELQNEELAL
HHHHHHHHHCCCEEEEECCCCCHHHCCEEEEECCCCEEEECCHHHHHHHHHHHCCHHHHH
SELITQDLDKPTFTRDEHQMKIYLNAGLSADTSIAVNQGVDGVGLYRTEIPFLLQQRFPS
HHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCEEECCCCCCCEEEHHHHHHHHHHHCCC
EEEQYLQYKTILETYPKKPVIMRTLDIGGDKPLPYLPIEEENPFLGWRGIRFTLDHPDIF
CHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCEEECCEEEEECCCCCH
LLQLKAMVRANIGNGNLSIMLPMISGLPELKQARKLINQAYEEVLGESEGDFSQPRIGMM
HHHHHHHHHHCCCCCCEEEEEEHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEE
IEVPSMLYLLPRVVGLVDFISVGTNDLTQYLLAVDRNNARVSHVYESYHPAVLLALKQII
EEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHCHHHHHHHHHHH
DTANQYKLPVSVCGELAGDPIGCLLLLGLGYQSLSMNTSNVAKVKYIIRQVSQQEVEQLV
CCHHCCCCCHHHHHHHCCCHHHHHHHHHCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
ATLLHHDDAEQILTDMIGFFEEKDLSGFIRAGR
HHHHCCCCHHHHHHHHHHHHHHCCCHHHHCCCC
>Mature Secondary Structure
MLTQLRDIVDHVAKAESLDDALALLVKNTRHSMHTECCSVYLMSNDNLRLELMATEGLKA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCEEEEEEECCCCCC
KPKTVSLGIDEGLVGLVARSCEPLNLANASVHPSYKYIPSIAEYKFNSFLATPIIYRRQN
CCCEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHCHHHEEECC
LGVLVVQQKEKRQFTEVEESFLVTLSAQLAVILAHAKAHESWSTQLQPQSIEQKQFKGIS
CCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCC
ASNGVAIAPLWFDDAQPRIESILPSSCLDTEQEQDRLGTAVELALKDFRRAKKRFEQELN
CCCCEEEEEEECCCCCHHHHHHCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TETLAIFDLFTHLLNDPMLKGDLKKQINQGDSAEWALRQVIESYAGRFEKMSDSYLSERA
CHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NDVRELGQRLLYFLNYEGKLELDLAHPVILVTTQLTASMLASVPQDKLKAVISLEGAANS
HHHHHHHHHHHHHCCCCCEEEEEECCCEEEEEHHHHHHHHHHCCHHHHHEEEEECCCCCC
HAAILSRALGIPAIMGVEFNPTHYHQNLAIVDGYSGALYIKPTEALIQEYTELQNEELAL
HHHHHHHHHCCCEEEEECCCCCHHHCCEEEEECCCCEEEECCHHHHHHHHHHHCCHHHHH
SELITQDLDKPTFTRDEHQMKIYLNAGLSADTSIAVNQGVDGVGLYRTEIPFLLQQRFPS
HHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCEEECCCCCCCEEEHHHHHHHHHHHCCC
EEEQYLQYKTILETYPKKPVIMRTLDIGGDKPLPYLPIEEENPFLGWRGIRFTLDHPDIF
CHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCEEECCEEEEECCCCCH
LLQLKAMVRANIGNGNLSIMLPMISGLPELKQARKLINQAYEEVLGESEGDFSQPRIGMM
HHHHHHHHHHCCCCCCEEEEEEHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEE
IEVPSMLYLLPRVVGLVDFISVGTNDLTQYLLAVDRNNARVSHVYESYHPAVLLALKQII
EEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHCHHHHHHHHHHH
DTANQYKLPVSVCGELAGDPIGCLLLLGLGYQSLSMNTSNVAKVKYIIRQVSQQEVEQLV
CCHHCCCCCHHHHHHHCCCHHHHHHHHHCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
ATLLHHDDAEQILTDMIGFFEEKDLSGFIRAGR
HHHHCCCCHHHHHHHHHHHHHHCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 7896715; 8973315 [H]