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The map label for this gene is vpsR [H]

Identifier: 59711061

GI number: 59711061

Start: 484262

End: 485599

Strand: Direct

Name: vpsR [H]

Synonym: VF_0454

Alternate gene names: 59711061

Gene position: 484262-485599 (Clockwise)

Preceding gene: 59711060

Following gene: 59711063

Centisome position: 16.71

GC content: 38.27

Gene sequence:

>1338_bases
ATGGGTACTCAATTTCGTATGGATTCGGTTCCAGGCTCTTTAGTTGTTGTAGGTGGCAGCTATGAACCTTGGTTAGCCGT
TTTAGAACAAGTAGGTTGGCGTTGTCATCGTTGTTATGATCTTCGAGCAGCTCAAGCTCTGTTTGACGAGATTGGGCCAT
GTATTGGTATTGTTGATTTAAGCCAAGATGATTTTAGTCTTAATGGCATTGCAACGTTAGTTAATAACAATAAGCAAGTT
CGCTGGATGGCTTTCATTCGTGAATCTCAATTAAGCTCAGATACTATCTGCCAGTTTATTGTTAACTTCTGTATTGATTT
TTTCACTGCACCAATTCCTGATGCTCAGCTATTAAGCACGATTGGTCACCAGTTGGGTATGCTTAAGTTAGAACGCAAAG
TATGGCCAAATATGGGGAATAAATCGGATCTTGGTATCTTGGGTGAAGCTCCAGCTGTTAAGCGCCTGCGAGATCAAATT
CGTCGTGTTGCTCCAACAGATGTTTCTATTCTTATTTCTGGTGAAAGTGGTGTAGGTAAAGATGCAGTAGCAAAAGCAGT
TCATGAATCTTCTGGTCGTAATAAAGGCCCATTTGTTTCATTAAATTGTGGTTCACTATCTGAAAATAAAATTGAACAAG
AACTGTTTGGTTCTCACTCTTCGTCACCAGAAGAATCTAAACTATTCCAAGCCAATGGTGGTACATTATTTTTAAATGAT
ATTAATGATTTGCCAGCTAGTCAGCAGCAACATCTATTACGATTCTTACAAGAAGGTAATATCGAGACTGAAAAAGGTTT
TGTTGATTTAGATGTTCGAGTAATTGTTGCAAGCCACATCGATTTAGAAAAAGCGATTGAAGAAGGCAGCTTTAAAGAAG
AGTTGTATTATCGCTTAAATGTATTGCGTATTCATGTACCATCTCTAAAAGAGAGAAGTTCAGATATCTCGGTTTTAGCA
GAGCATTTCTTACAGCGTTATGCAAAAGAGTACAATACACAAGCAAGAACGTTCTCAGAAGAAGCATTACAAACGATGTT
GCATTATCAATGGCCTGGTAATGTACGTGAGTTAGTAAACCAAGTGAAACGTGCTGTATTACTTGCAGATAGCATGACGA
TTGATCTTGAGCACCTAGACTTACCGCAACGCAGTGAAACAAAACGCAGTTTGCGTGTTATCCGTGAAAATTCGGAGAGA
GATGCGTTATTAATGGTATTAGAATCTCATGATGGTCAAGTTTCTTCAGCTGCAAAAGAATTAGGTGTTTCACGAGCAAC
TATGTATCGTTTATTAAATAAACATAATTTGATTTCTGAGCCGAGAAGCTATAGCTAA

Upstream 100 bases:

>100_bases
GCATCTCATTTGTGAGACGAGATAAGGTCTTAAAACCTGTATAGTTGCAACTAAGAAACATTTTAAGTTGGTTTAGATAT
AAGAATTTAGAGGTAGTGTT

Downstream 100 bases:

>100_bases
TCGAATCCTTATTGAAGAATAGGCTTACCTTGAATTAGGTAGGCCTATTTTTTTAGTTGAAGAATGAAGTCAGTATAGAA
TGAGTAAATTGAGTTTTTAA

Product: sigma-54 dependent transcriptional regulator VpsR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MGTQFRMDSVPGSLVVVGGSYEPWLAVLEQVGWRCHRCYDLRAAQALFDEIGPCIGIVDLSQDDFSLNGIATLVNNNKQV
RWMAFIRESQLSSDTICQFIVNFCIDFFTAPIPDAQLLSTIGHQLGMLKLERKVWPNMGNKSDLGILGEAPAVKRLRDQI
RRVAPTDVSILISGESGVGKDAVAKAVHESSGRNKGPFVSLNCGSLSENKIEQELFGSHSSSPEESKLFQANGGTLFLND
INDLPASQQQHLLRFLQEGNIETEKGFVDLDVRVIVASHIDLEKAIEEGSFKEELYYRLNVLRIHVPSLKERSSDISVLA
EHFLQRYAKEYNTQARTFSEEALQTMLHYQWPGNVRELVNQVKRAVLLADSMTIDLEHLDLPQRSETKRSLRVIRENSER
DALLMVLESHDGQVSSAAKELGVSRATMYRLLNKHNLISEPRSYS

Sequences:

>Translated_445_residues
MGTQFRMDSVPGSLVVVGGSYEPWLAVLEQVGWRCHRCYDLRAAQALFDEIGPCIGIVDLSQDDFSLNGIATLVNNNKQV
RWMAFIRESQLSSDTICQFIVNFCIDFFTAPIPDAQLLSTIGHQLGMLKLERKVWPNMGNKSDLGILGEAPAVKRLRDQI
RRVAPTDVSILISGESGVGKDAVAKAVHESSGRNKGPFVSLNCGSLSENKIEQELFGSHSSSPEESKLFQANGGTLFLND
INDLPASQQQHLLRFLQEGNIETEKGFVDLDVRVIVASHIDLEKAIEEGSFKEELYYRLNVLRIHVPSLKERSSDISVLA
EHFLQRYAKEYNTQARTFSEEALQTMLHYQWPGNVRELVNQVKRAVLLADSMTIDLEHLDLPQRSETKRSLRVIRENSER
DALLMVLESHDGQVSSAAKELGVSRATMYRLLNKHNLISEPRSYS
>Mature_444_residues
GTQFRMDSVPGSLVVVGGSYEPWLAVLEQVGWRCHRCYDLRAAQALFDEIGPCIGIVDLSQDDFSLNGIATLVNNNKQVR
WMAFIRESQLSSDTICQFIVNFCIDFFTAPIPDAQLLSTIGHQLGMLKLERKVWPNMGNKSDLGILGEAPAVKRLRDQIR
RVAPTDVSILISGESGVGKDAVAKAVHESSGRNKGPFVSLNCGSLSENKIEQELFGSHSSSPEESKLFQANGGTLFLNDI
NDLPASQQQHLLRFLQEGNIETEKGFVDLDVRVIVASHIDLEKAIEEGSFKEELYYRLNVLRIHVPSLKERSSDISVLAE
HFLQRYAKEYNTQARTFSEEALQTMLHYQWPGNVRELVNQVKRAVLLADSMTIDLEHLDLPQRSETKRSLRVIRENSERD
ALLMVLESHDGQVSSAAKELGVSRATMYRLLNKHNLISEPRSYS

Specific function: Member of the two-component regulatory system zraS/zraR. When activated by zraS it acts in conjunction with sigma-54 to regulate the expression of zraP. Positively autoregulates the expression of the zraSR operon [H]

COG id: COG1221

COG function: function code KT; Transcriptional regulators containing an AAA-type ATPase domain and a DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790437, Length=386, Percent_Identity=34.7150259067358, Blast_Score=232, Evalue=5e-62,
Organism=Escherichia coli, GI1788905, Length=348, Percent_Identity=37.6436781609195, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI1788550, Length=428, Percent_Identity=32.4766355140187, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI1790299, Length=334, Percent_Identity=35.3293413173653, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI87082117, Length=321, Percent_Identity=36.1370716510903, Blast_Score=187, Evalue=9e-49,
Organism=Escherichia coli, GI1786524, Length=314, Percent_Identity=35.6687898089172, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI1789087, Length=303, Percent_Identity=35.6435643564356, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI1789233, Length=321, Percent_Identity=33.0218068535826, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI87082152, Length=316, Percent_Identity=33.5443037974684, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI1787583, Length=305, Percent_Identity=34.0983606557377, Blast_Score=171, Evalue=7e-44,
Organism=Escherichia coli, GI87081872, Length=318, Percent_Identity=33.6477987421384, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI87081858, Length=300, Percent_Identity=29.3333333333333, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1789828, Length=251, Percent_Identity=31.8725099601594, Blast_Score=109, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 49927; Mature: 49796

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTQFRMDSVPGSLVVVGGSYEPWLAVLEQVGWRCHRCYDLRAAQALFDEIGPCIGIVDL
CCCCEECCCCCCCEEEECCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCEEEEEEC
SQDDFSLNGIATLVNNNKQVRWMAFIRESQLSSDTICQFIVNFCIDFFTAPIPDAQLLST
CCCCCCCCCEEEEECCCCEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHH
IGHQLGMLKLERKVWPNMGNKSDLGILGEAPAVKRLRDQIRRVAPTDVSILISGESGVGK
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCH
DAVAKAVHESSGRNKGPFVSLNCGSLSENKIEQELFGSHSSSPEESKLFQANGGTLFLND
HHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCHHHEEECCCCEEEEEC
INDLPASQQQHLLRFLQEGNIETEKGFVDLDVRVIVASHIDLEKAIEEGSFKEELYYRLN
CCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEHHCCHHHHHHCCCHHHHHHHHEE
VLRIHVPSLKERSSDISVLAEHFLQRYAKEYNTQARTFSEEALQTMLHYQWPGNVRELVN
EEEEECCCHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHH
QVKRAVLLADSMTIDLEHLDLPQRSETKRSLRVIRENSERDALLMVLESHDGQVSSAAKE
HHHHHHHHHCCCEEEHHHCCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHH
LGVSRATMYRLLNKHNLISEPRSYS
HCCHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
GTQFRMDSVPGSLVVVGGSYEPWLAVLEQVGWRCHRCYDLRAAQALFDEIGPCIGIVDL
CCCEECCCCCCCEEEECCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCEEEEEEC
SQDDFSLNGIATLVNNNKQVRWMAFIRESQLSSDTICQFIVNFCIDFFTAPIPDAQLLST
CCCCCCCCCEEEEECCCCEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHH
IGHQLGMLKLERKVWPNMGNKSDLGILGEAPAVKRLRDQIRRVAPTDVSILISGESGVGK
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCH
DAVAKAVHESSGRNKGPFVSLNCGSLSENKIEQELFGSHSSSPEESKLFQANGGTLFLND
HHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCHHHEEECCCCEEEEEC
INDLPASQQQHLLRFLQEGNIETEKGFVDLDVRVIVASHIDLEKAIEEGSFKEELYYRLN
CCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEHHCCHHHHHHCCCHHHHHHHHEE
VLRIHVPSLKERSSDISVLAEHFLQRYAKEYNTQARTFSEEALQTMLHYQWPGNVRELVN
EEEEECCCHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHH
QVKRAVLLADSMTIDLEHLDLPQRSETKRSLRVIRENSERDALLMVLESHDGQVSSAAKE
HHHHHHHHHCCCEEEHHHCCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHH
LGVSRATMYRLLNKHNLISEPRSYS
HCCHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]