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The map label for this gene is lysS [H]

Identifier: 59711060

GI number: 59711060

Start: 482369

End: 483874

Strand: Direct

Name: lysS [H]

Synonym: VF_0453

Alternate gene names: 59711060

Gene position: 482369-483874 (Clockwise)

Preceding gene: 172087665

Following gene: 59711061

Centisome position: 16.65

GC content: 41.3

Gene sequence:

>1506_bases
ATGACTGAACAAGTACAACTAGATGAAAACAAACTGATTGCTGAACGTCGTGGTAAATTAGACCATATTCGTCAAGCATG
TAAAGCTAATGGCCACCCAAATGACTTCCGTCGTGATAGCCTAGCGGGTGATCTTCAAGCTGAATTTGGTGAAAAGACCA
AAGAAGAGCTAGAAGAACTGAACCACGTTGTTGCAATTGCTGGCCGTGTTATGGCTAAGCGTGGTCCTTTCTTATTGATT
CAAGAAGTATCTGGTAAGATCCAAGCTTACGCAGCAAAAGAAGTTCAAAAAGAACTAAAAGACAAATACCAAGGTTTGGA
TATCGGTGACATCATCGGTGTTAAAGGTGCACTTCATAAGTCAGGTAAAGGTGATCTTTACGTGAACATGGAAGAGTATG
TACTGCTAACAAAAGCATTACGTCCATTACCAGAAAAATTCCATGGTTTAACAGACCAAGAGATGCGTTACCGTCAACGT
TACGTAGATCTAATCGTAAATGATGACTCACGTCATGCATTTATCGTTCGTTCTAAAGTGGTTGCTGCTATCCGTAACTT
CATGGTTGATAAAGGCTTCATGGAAGTTGAAACTCCTATGATGCACGTTATCCCTGGCGGTGCTTCTGCGCGTCCATTCG
TAACGCACCATAATGCACTTGATGTTGATATGTACCTACGTATTGCTCCTGAGCTTTACTTAAAACGTCTAGTTGTTGGT
GGTTTTGAACGTGTATTCGAAATTAACCGTAACTTCCGTAACGAAGGTCTATCTCCACGTCATAACCCAGAATTCACAAT
GATGGAATTCTACATGGCGTATGCAGATTACAACGATCTAATGGACCTTACAGAAGAAATGCTATCTACAGTAGCAACAT
CTGTTTTAGGTAGCGACAAAATGCCTTATGGTGAGCACACGGTTGATTTTGGTGGCAAATACGCTCGTATGAGCATGTTA
GATGCGATCAAACAATACAACCCTGATCACGCAGAGATCCAAGCGCTAACGTATGAAGGCGTTAAAGATCGCGATCTAAT
GGTATCTATTGCTAAATCTGTACATGTTGACGTTGAAAGCTTCTGGACATGTGGTCAGCTTCTTGAAGAGATCTTCGGTG
AAACTGCTGAACCTAAGCTAATGCAACCTACATTCATTACTGAATACCCAGCAGATATTTCTCCATTAGCACGTCGTAAC
GATGACAATGATTTCATTACTGATCGTTTTGAATTCTTCATTGGTGGTCGTGAAGTAGCAAATGGTTTCTCTGAGCTGAA
TGATGCACAAGATCAAGACGAGCGTTTTAAAGCGCAAGTTAATGCTAAAGAATCTGGTGATGATGAAGCTATGTACTACG
ATGCAGACTACATCACAGCTCTTGAGCATGGTTTACCACCAACTGCTGGTCAAGGTATCGGTATTGACCGTTTAGTTATG
CTATTAACTAACACGCACACAATTCGTGACGTTATTTTATTCCCATCAATGCGTCCACAAGCGTAA

Upstream 100 bases:

>100_bases
ACCGGCATTGAAAACCGTAATACGCAAGCCGTTCTAGACGGTGATTTAGATAAATTTATCGAAGCCAGCTTAAAATCTGG
ATTATAAGAAAGGGTTCATT

Downstream 100 bases:

>100_bases
TAAAGAATAAATAAAGATGATGAAAGAGCGGCTAATTAGCCGCTCTTTTTTTATTTATCTTACTCAAAGCACTAAAAATG
AGAAAATAACTACACTTTTT

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 501; Mature: 500

Protein sequence:

>501_residues
MTEQVQLDENKLIAERRGKLDHIRQACKANGHPNDFRRDSLAGDLQAEFGEKTKEELEELNHVVAIAGRVMAKRGPFLLI
QEVSGKIQAYAAKEVQKELKDKYQGLDIGDIIGVKGALHKSGKGDLYVNMEEYVLLTKALRPLPEKFHGLTDQEMRYRQR
YVDLIVNDDSRHAFIVRSKVVAAIRNFMVDKGFMEVETPMMHVIPGGASARPFVTHHNALDVDMYLRIAPELYLKRLVVG
GFERVFEINRNFRNEGLSPRHNPEFTMMEFYMAYADYNDLMDLTEEMLSTVATSVLGSDKMPYGEHTVDFGGKYARMSML
DAIKQYNPDHAEIQALTYEGVKDRDLMVSIAKSVHVDVESFWTCGQLLEEIFGETAEPKLMQPTFITEYPADISPLARRN
DDNDFITDRFEFFIGGREVANGFSELNDAQDQDERFKAQVNAKESGDDEAMYYDADYITALEHGLPPTAGQGIGIDRLVM
LLTNTHTIRDVILFPSMRPQA

Sequences:

>Translated_501_residues
MTEQVQLDENKLIAERRGKLDHIRQACKANGHPNDFRRDSLAGDLQAEFGEKTKEELEELNHVVAIAGRVMAKRGPFLLI
QEVSGKIQAYAAKEVQKELKDKYQGLDIGDIIGVKGALHKSGKGDLYVNMEEYVLLTKALRPLPEKFHGLTDQEMRYRQR
YVDLIVNDDSRHAFIVRSKVVAAIRNFMVDKGFMEVETPMMHVIPGGASARPFVTHHNALDVDMYLRIAPELYLKRLVVG
GFERVFEINRNFRNEGLSPRHNPEFTMMEFYMAYADYNDLMDLTEEMLSTVATSVLGSDKMPYGEHTVDFGGKYARMSML
DAIKQYNPDHAEIQALTYEGVKDRDLMVSIAKSVHVDVESFWTCGQLLEEIFGETAEPKLMQPTFITEYPADISPLARRN
DDNDFITDRFEFFIGGREVANGFSELNDAQDQDERFKAQVNAKESGDDEAMYYDADYITALEHGLPPTAGQGIGIDRLVM
LLTNTHTIRDVILFPSMRPQA
>Mature_500_residues
TEQVQLDENKLIAERRGKLDHIRQACKANGHPNDFRRDSLAGDLQAEFGEKTKEELEELNHVVAIAGRVMAKRGPFLLIQ
EVSGKIQAYAAKEVQKELKDKYQGLDIGDIIGVKGALHKSGKGDLYVNMEEYVLLTKALRPLPEKFHGLTDQEMRYRQRY
VDLIVNDDSRHAFIVRSKVVAAIRNFMVDKGFMEVETPMMHVIPGGASARPFVTHHNALDVDMYLRIAPELYLKRLVVGG
FERVFEINRNFRNEGLSPRHNPEFTMMEFYMAYADYNDLMDLTEEMLSTVATSVLGSDKMPYGEHTVDFGGKYARMSMLD
AIKQYNPDHAEIQALTYEGVKDRDLMVSIAKSVHVDVESFWTCGQLLEEIFGETAEPKLMQPTFITEYPADISPLARRND
DNDFITDRFEFFIGGREVANGFSELNDAQDQDERFKAQVNAKESGDDEAMYYDADYITALEHGLPPTAGQGIGIDRLVML
LTNTHTIRDVILFPSMRPQA

Specific function: Also Can Synthesize A Number Of Adenyl Dinucleotides (In Particular Appppa). These Dinucleotides Have Been Proposed To Act As Modulators Of The Heat-Shock Response And Stress Response. [C]

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=492, Percent_Identity=42.6829268292683, Blast_Score=385, Evalue=1e-107,
Organism=Homo sapiens, GI194272210, Length=492, Percent_Identity=42.6829268292683, Blast_Score=385, Evalue=1e-107,
Organism=Homo sapiens, GI40789249, Length=246, Percent_Identity=25.609756097561, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1790571, Length=500, Percent_Identity=62, Blast_Score=637, Evalue=0.0,
Organism=Escherichia coli, GI1789256, Length=499, Percent_Identity=60.9218436873748, Blast_Score=620, Evalue=1e-179,
Organism=Escherichia coli, GI87082379, Length=332, Percent_Identity=31.6265060240964, Blast_Score=156, Evalue=3e-39,
Organism=Escherichia coli, GI1788173, Length=194, Percent_Identity=25.7731958762887, Blast_Score=72, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17535925, Length=504, Percent_Identity=39.484126984127, Blast_Score=362, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI17535927, Length=504, Percent_Identity=39.484126984127, Blast_Score=362, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI71994340, Length=504, Percent_Identity=39.2857142857143, Blast_Score=362, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI32566633, Length=283, Percent_Identity=27.5618374558304, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17551876, Length=355, Percent_Identity=25.3521126760563, Blast_Score=88, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6320242, Length=523, Percent_Identity=40.1529636711281, Blast_Score=361, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6324256, Length=388, Percent_Identity=30.6701030927835, Blast_Score=173, Evalue=6e-44,
Organism=Saccharomyces cerevisiae, GI6323011, Length=329, Percent_Identity=27.0516717325228, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24640851, Length=519, Percent_Identity=40.4624277456647, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24640849, Length=519, Percent_Identity=40.4624277456647, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24584738, Length=245, Percent_Identity=28.1632653061224, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI17136276, Length=464, Percent_Identity=23.2758620689655, Blast_Score=90, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 56898; Mature: 56767

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEQVQLDENKLIAERRGKLDHIRQACKANGHPNDFRRDSLAGDLQAEFGEKTKEELEEL
CCCCEECCCHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHCCHHHHHHCHHHHHHHHHH
NHVVAIAGRVMAKRGPFLLIQEVSGKIQAYAAKEVQKELKDKYQGLDIGDIIGVKGALHK
HHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHCC
SGKGDLYVNMEEYVLLTKALRPLPEKFHGLTDQEMRYRQRYVDLIVNDDSRHAFIVRSKV
CCCCCEEECHHHHHHHHHHHHHCHHHHCCCCHHHHHHHHHHHEEEECCCCCEEEEHHHHH
VAAIRNFMVDKGFMEVETPMMHVIPGGASARPFVTHHNALDVDMYLRIAPELYLKRLVVG
HHHHHHHHHCCCCHHHCCCEEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHH
GFERVFEINRNFRNEGLSPRHNPEFTMMEFYMAYADYNDLMDLTEEMLSTVATSVLGSDK
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCC
MPYGEHTVDFGGKYARMSMLDAIKQYNPDHAEIQALTYEGVKDRDLMVSIAKSVHVDVES
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHEEEEEECCCCCHHHHHHHHHHHCCCHHH
FWTCGQLLEEIFGETAEPKLMQPTFITEYPADISPLARRNDDNDFITDRFEFFIGGREVA
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHCCCHHHH
NGFSELNDAQDQDERFKAQVNAKESGDDEAMYYDADYITALEHGLPPTAGQGIGIDRLVM
HHHHHHCCCCCHHHHHHHHCCCCCCCCCCEEEECHHHHHHHHHCCCCCCCCCCCHHHHHH
LLTNTHTIRDVILFPSMRPQA
HHHCCHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TEQVQLDENKLIAERRGKLDHIRQACKANGHPNDFRRDSLAGDLQAEFGEKTKEELEEL
CCCEECCCHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHCCHHHHHHCHHHHHHHHHH
NHVVAIAGRVMAKRGPFLLIQEVSGKIQAYAAKEVQKELKDKYQGLDIGDIIGVKGALHK
HHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHCC
SGKGDLYVNMEEYVLLTKALRPLPEKFHGLTDQEMRYRQRYVDLIVNDDSRHAFIVRSKV
CCCCCEEECHHHHHHHHHHHHHCHHHHCCCCHHHHHHHHHHHEEEECCCCCEEEEHHHHH
VAAIRNFMVDKGFMEVETPMMHVIPGGASARPFVTHHNALDVDMYLRIAPELYLKRLVVG
HHHHHHHHHCCCCHHHCCCEEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHH
GFERVFEINRNFRNEGLSPRHNPEFTMMEFYMAYADYNDLMDLTEEMLSTVATSVLGSDK
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCC
MPYGEHTVDFGGKYARMSMLDAIKQYNPDHAEIQALTYEGVKDRDLMVSIAKSVHVDVES
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHEEEEEECCCCCHHHHHHHHHHHCCCHHH
FWTCGQLLEEIFGETAEPKLMQPTFITEYPADISPLARRNDDNDFITDRFEFFIGGREVA
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHCCCHHHH
NGFSELNDAQDQDERFKAQVNAKESGDDEAMYYDADYITALEHGLPPTAGQGIGIDRLVM
HHHHHHCCCCCHHHHHHHHCCCCCCCCCCEEEECHHHHHHHHHCCCCCCCCCCCHHHHHH
LLTNTHTIRDVILFPSMRPQA
HHHCCHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA