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The map label for this gene is dsbC [H]

Identifier: 59711056

GI number: 59711056

Start: 478401

End: 479177

Strand: Direct

Name: dsbC [H]

Synonym: VF_0449

Alternate gene names: 59711056

Gene position: 478401-479177 (Clockwise)

Preceding gene: 59711055

Following gene: 59711057

Centisome position: 16.51

GC content: 37.71

Gene sequence:

>777_bases
ATGTCATTTATTCGCCGAACTAGTGCGATGATCATTGCTTTAATGAGCGTAGTTTCTTTTACTGCATGTTCAGATGAACA
AGCAAAAAACCAGGATGCGTCACCTTCTGCTGTTGAGGCTGCTCAACCTGATGCAGAACAAGCTATTACTCAGCGACTAA
CGACTTTAGGTTTGCCTGTAGAAGCTATCCATGATTCTGAAGTAGATGGTTTTAAACAAGTTGAAACACCGTTTGGGATT
TTTTACGTATCTTCAGATGGTAAGCATTTTATTCAAGGTCGAATTTTTGAGTTTGATGAAAATGGTGACATGAAAGATTT
ATTAGCTGCTCGTTTTGCTAAATTGGTGGATAGCCAAAGCGAAAATATGATTGTATTCCCAGCTAAAAACGAAAAACACG
TTATTACTGTATTTACAGATATTACTTGTGGTTACTGTACAAAACTGCATAAAGAGATGAAAGACTATAATGATGCGGGT
ATTACCGTTCGTTATTTAGCATACCCTCGCCAAGGCTACCAAGGATCAGTTGCTGATAAAATGGCTCAAATTTGGTGTGC
TGATGATAAACAGCAAGCAATGGAAGATGCAAAATCAAATCGTCCAATTAATGGTTCAAAACCTGCAACTGCAGAGTGTA
AAAACATCATTAAAGAACAGTATTTATTAGGCAGAAAGATGGGTGTAAATGGAACACCAGCAATCGTTCTTCCAAGTGGT
GATCTTGTTCCTGGATACAAACCAGCACGAGAGCTTCTATCTGATTTAGAAAAATAA

Upstream 100 bases:

>100_bases
ATCAAGAGCATCACCCTCGCTCATAATTGAGGGAACTTCTGGTGGTTTAAAAACTCTAAACTTCAAATCTTCTACAATTA
CATTTTTTATAGGTACCCTT

Downstream 100 bases:

>100_bases
TTCAGTAAAGCCATTCATTTTAATCCCCGTATATGCGGGGATTTTTTTATTGAAAAACTAGGTGAAATTCATGATTGAAG
TGAAACGTCGAACAGTACCA

Product: protein disulfide isomerase II

Products: 3-oxo-D 4-steroid; protein with oxidized disulfide bonds [C]

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MSFIRRTSAMIIALMSVVSFTACSDEQAKNQDASPSAVEAAQPDAEQAITQRLTTLGLPVEAIHDSEVDGFKQVETPFGI
FYVSSDGKHFIQGRIFEFDENGDMKDLLAARFAKLVDSQSENMIVFPAKNEKHVITVFTDITCGYCTKLHKEMKDYNDAG
ITVRYLAYPRQGYQGSVADKMAQIWCADDKQQAMEDAKSNRPINGSKPATAECKNIIKEQYLLGRKMGVNGTPAIVLPSG
DLVPGYKPARELLSDLEK

Sequences:

>Translated_258_residues
MSFIRRTSAMIIALMSVVSFTACSDEQAKNQDASPSAVEAAQPDAEQAITQRLTTLGLPVEAIHDSEVDGFKQVETPFGI
FYVSSDGKHFIQGRIFEFDENGDMKDLLAARFAKLVDSQSENMIVFPAKNEKHVITVFTDITCGYCTKLHKEMKDYNDAG
ITVRYLAYPRQGYQGSVADKMAQIWCADDKQQAMEDAKSNRPINGSKPATAECKNIIKEQYLLGRKMGVNGTPAIVLPSG
DLVPGYKPARELLSDLEK
>Mature_257_residues
SFIRRTSAMIIALMSVVSFTACSDEQAKNQDASPSAVEAAQPDAEQAITQRLTTLGLPVEAIHDSEVDGFKQVETPFGIF
YVSSDGKHFIQGRIFEFDENGDMKDLLAARFAKLVDSQSENMIVFPAKNEKHVITVFTDITCGYCTKLHKEMKDYNDAGI
TVRYLAYPRQGYQGSVADKMAQIWCADDKQQAMEDAKSNRPINGSKPATAECKNIIKEQYLLGRKMGVNGTPAIVLPSGD
LVPGYKPARELLSDLEK

Specific function: Required for disulfide bond formation in some periplasmic proteins. Acts by transferring its disulfide bond to other proteins and is reduced in the process. DsbC is reoxidized by a yet uncharacterized protein. Also acts as a disulfide isomerase [H]

COG id: COG1651

COG function: function code O; Protein-disulfide isomerase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain [H]

Homologues:

Organism=Escherichia coli, GI1789260, Length=212, Percent_Identity=44.811320754717, Blast_Score=188, Evalue=3e-49,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018950
- InterPro:   IPR009094
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR017937
- InterPro:   IPR012335 [H]

Pfam domain/function: PF10411 DsbC_N [H]

EC number: NA

Molecular weight: Translated: 28486; Mature: 28354

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00194 THIOREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFIRRTSAMIIALMSVVSFTACSDEQAKNQDASPSAVEAAQPDAEQAITQRLTTLGLPV
CCHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCHHHHCCCCHHHHHHHHHHHHCCCH
EAIHDSEVDGFKQVETPFGIFYVSSDGKHFIQGRIFEFDENGDMKDLLAARFAKLVDSQS
HHHCCCCCCCHHHHCCCEEEEEECCCCCEEEECEEEEECCCCCHHHHHHHHHHHHHCCCC
ENMIVFPAKNEKHVITVFTDITCGYCTKLHKEMKDYNDAGITVRYLAYPRQGYQGSVADK
CCEEEEECCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHHH
MAQIWCADDKQQAMEDAKSNRPINGSKPATAECKNIIKEQYLLGRKMGVNGTPAIVLPSG
HHHHHCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCC
DLVPGYKPARELLSDLEK
CCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SFIRRTSAMIIALMSVVSFTACSDEQAKNQDASPSAVEAAQPDAEQAITQRLTTLGLPV
CHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCHHHHCCCCHHHHHHHHHHHHCCCH
EAIHDSEVDGFKQVETPFGIFYVSSDGKHFIQGRIFEFDENGDMKDLLAARFAKLVDSQS
HHHCCCCCCCHHHHCCCEEEEEECCCCCEEEECEEEEECCCCCHHHHHHHHHHHHHCCCC
ENMIVFPAKNEKHVITVFTDITCGYCTKLHKEMKDYNDAGITVRYLAYPRQGYQGSVADK
CCEEEEECCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHHH
MAQIWCADDKQQAMEDAKSNRPINGSKPATAECKNIIKEQYLLGRKMGVNGTPAIVLPSG
HHHHHCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCC
DLVPGYKPARELLSDLEK
CCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-oxo-D 5-steroid; protein with reduced SH groups [C]

Specific reaction: 3-oxo-D 5-steroid = 3-oxo-D 4-steroid protein with reduced SH groups = protein with oxidized disulfide bonds [C]

General reaction: Isomerases; Intramolecular Oxidoreductases; Transposing S-S Bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8168497 [H]