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The map label for this gene is recJ [H]

Identifier: 59711057

GI number: 59711057

Start: 479248

End: 480987

Strand: Direct

Name: recJ [H]

Synonym: VF_0450

Alternate gene names: 59711057

Gene position: 479248-480987 (Clockwise)

Preceding gene: 59711056

Following gene: 172087665

Centisome position: 16.54

GC content: 40.4

Gene sequence:

>1740_bases
ATGATTGAAGTGAAACGTCGAACAGTACCATCCTCAACTCAACTTCCTGATTCGATACCTCCGATTTTGCAGCGAATTTA
TGCAGCGAGAGGGATTACATCAAGCTCGCAGCTTGAGCGTGGTGCAAAATTTTTACTATCATTTCAATCAATGCACGGTA
TTTCTAAAGCTGTAGATATCCTAGTTGAAGCAATTCAAAAGCAAACTCGTATTATCGTTGTTGGGGATTTTGATGCCGAT
GGTGCAACCAGTTCAGCATTATCTGTAATGGCATTTCGTATGATGGGAAGTCAAAATGTCGATTACTTGGTGCCTAACCG
ATTTGAAGACGGCTATGGCTTGAGTCCTGATGTTGTGGATCAAGCAAAAGAGATGGGTGCTGAGATCATCATGACCGTTG
ATAATGGTGTTTCATCCATTGATGGTGTTTTGAAAGCAAAAGAATATGGTATGCAAGTTGTGGTAACGGATCATCACTTA
CCTGGTGCTCATTTGCCTCTTGCTGATGCGATTGTAAACCCTAATTTAGAGGCGTGCGCATTCCCATCTAAATCTTTAGC
AGGTGTAGGTGTTGCCTTTTATTTAATGCTTGCTATTCGTGCTCGTCTTCGTGAAATAAATTGGTTTGAAAAACAAAATA
TAGCGATACCGAATTTAGCTGATTTACTTGATTTAGTTGCGCTTGGGACTGTGGCTGACTTAGTTGCTTTGGATGAAAAT
AATCGAATTCTTGTTCATCAAGGTATTCAACGCATTCGCGCTGGTAAGTGTCGACCAGGGATCCAAGCGCTGATTGAAGT
GGCTAACCGAGTTCCATCACGTATTGTTGCTTCTGACTTTGGGTTTGCGTTAGGCCCAAGAATAAATGCAGCGGGTCGTT
TGGATGATATGTCATTTGGTGTTGAGCTTTTAATGTCAAATAATATTCATGCCGCTCGTCGTATGGCGGCAGAATTAGAT
TCATTAAATCAAACTCGAAAAGAAATTGAGCAGGGCATGAAAGAAGAAGCCATGGCTATTTGTGAGCGATTAGAGTTTGG
TGCTCAAAGTGAATTGCCTTTTGGATTGGTGTTGTTTCAGCGTGATTGGCACCAAGGTGTTATTGGTATTTTGGCTTCAC
GTATAAAAGAAAAATATCATCGTCCTGTCATTGCTTTTGCTGACGGTGGTGATGGTTTTATTAAAGGATCATGCCGCTCT
ATTGCAGGCTTTCATATGCGTGATGCATTAGATTTGATTGATACTAGAAATCCAGGCCTTATTTTGAAATTTGGTGGACA
TGCTATGGCTGCTGGATTAACCATTAAAGAGGCGGATTACAAAAAATTCAGTCAAGCTTTTGATGATGTCGTCCGTGAGT
CGGTAGAAGAAGAAGCTCTTAAAGGGATTATTTTATCTGATGGTGCACTAACACCAGAAGAATTTACATTAGGTACGGCT
GAAATTATCCGAGCGGGTGGACCATGGGGACAAGCTTTTCCTGAGCCTATTTTTGATGGTGAATTTAAAGTATTAAACCA
ACGTCTAGTGGGCGAAAAACATCTTAAATTAATGTTAGAGCCGATTCACCGTGATTTTCCAACCAATAAAATGGTTGATG
CTATCGCATTTAATATTGATGTTCGCCGTTGGCCAGATGCTTCAGTTCAAAAAGTAAAGTTAGCTTATCGACTCGATATT
AATGAATTTCGTGGAAATCAGACCTTACAGCTTATGGTTGAGAACATTGAACCACTTTAA

Upstream 100 bases:

>100_bases
CGAGAGCTTCTATCTGATTTAGAAAAATAATTCAGTAAAGCCATTCATTTTAATCCCCGTATATGCGGGGATTTTTTTAT
TGAAAAACTAGGTGAAATTC

Downstream 100 bases:

>100_bases
TTCATGAATAATCCGTAAAACACGTTAATTTTCACTGAACAACTCATTAAAAAAGACTTAATGAGTTGTTATGTTTATGG
TCTAGTTGCAAACAGATAGG

Product: ssDNA exonuclease RecJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 579; Mature: 579

Protein sequence:

>579_residues
MIEVKRRTVPSSTQLPDSIPPILQRIYAARGITSSSQLERGAKFLLSFQSMHGISKAVDILVEAIQKQTRIIVVGDFDAD
GATSSALSVMAFRMMGSQNVDYLVPNRFEDGYGLSPDVVDQAKEMGAEIIMTVDNGVSSIDGVLKAKEYGMQVVVTDHHL
PGAHLPLADAIVNPNLEACAFPSKSLAGVGVAFYLMLAIRARLREINWFEKQNIAIPNLADLLDLVALGTVADLVALDEN
NRILVHQGIQRIRAGKCRPGIQALIEVANRVPSRIVASDFGFALGPRINAAGRLDDMSFGVELLMSNNIHAARRMAAELD
SLNQTRKEIEQGMKEEAMAICERLEFGAQSELPFGLVLFQRDWHQGVIGILASRIKEKYHRPVIAFADGGDGFIKGSCRS
IAGFHMRDALDLIDTRNPGLILKFGGHAMAAGLTIKEADYKKFSQAFDDVVRESVEEEALKGIILSDGALTPEEFTLGTA
EIIRAGGPWGQAFPEPIFDGEFKVLNQRLVGEKHLKLMLEPIHRDFPTNKMVDAIAFNIDVRRWPDASVQKVKLAYRLDI
NEFRGNQTLQLMVENIEPL

Sequences:

>Translated_579_residues
MIEVKRRTVPSSTQLPDSIPPILQRIYAARGITSSSQLERGAKFLLSFQSMHGISKAVDILVEAIQKQTRIIVVGDFDAD
GATSSALSVMAFRMMGSQNVDYLVPNRFEDGYGLSPDVVDQAKEMGAEIIMTVDNGVSSIDGVLKAKEYGMQVVVTDHHL
PGAHLPLADAIVNPNLEACAFPSKSLAGVGVAFYLMLAIRARLREINWFEKQNIAIPNLADLLDLVALGTVADLVALDEN
NRILVHQGIQRIRAGKCRPGIQALIEVANRVPSRIVASDFGFALGPRINAAGRLDDMSFGVELLMSNNIHAARRMAAELD
SLNQTRKEIEQGMKEEAMAICERLEFGAQSELPFGLVLFQRDWHQGVIGILASRIKEKYHRPVIAFADGGDGFIKGSCRS
IAGFHMRDALDLIDTRNPGLILKFGGHAMAAGLTIKEADYKKFSQAFDDVVRESVEEEALKGIILSDGALTPEEFTLGTA
EIIRAGGPWGQAFPEPIFDGEFKVLNQRLVGEKHLKLMLEPIHRDFPTNKMVDAIAFNIDVRRWPDASVQKVKLAYRLDI
NEFRGNQTLQLMVENIEPL
>Mature_579_residues
MIEVKRRTVPSSTQLPDSIPPILQRIYAARGITSSSQLERGAKFLLSFQSMHGISKAVDILVEAIQKQTRIIVVGDFDAD
GATSSALSVMAFRMMGSQNVDYLVPNRFEDGYGLSPDVVDQAKEMGAEIIMTVDNGVSSIDGVLKAKEYGMQVVVTDHHL
PGAHLPLADAIVNPNLEACAFPSKSLAGVGVAFYLMLAIRARLREINWFEKQNIAIPNLADLLDLVALGTVADLVALDEN
NRILVHQGIQRIRAGKCRPGIQALIEVANRVPSRIVASDFGFALGPRINAAGRLDDMSFGVELLMSNNIHAARRMAAELD
SLNQTRKEIEQGMKEEAMAICERLEFGAQSELPFGLVLFQRDWHQGVIGILASRIKEKYHRPVIAFADGGDGFIKGSCRS
IAGFHMRDALDLIDTRNPGLILKFGGHAMAAGLTIKEADYKKFSQAFDDVVRESVEEEALKGIILSDGALTPEEFTLGTA
EIIRAGGPWGQAFPEPIFDGEFKVLNQRLVGEKHLKLMLEPIHRDFPTNKMVDAIAFNIDVRRWPDASVQKVKLAYRLDI
NEFRGNQTLQLMVENIEPL

Specific function: Single-stranded-DNA-specific exonuclease. Required for many types of recombinational events, although the stringency of the requirement for recJ appears to vary with the type of recombinational event monitored and the other recombination gene products whi

COG id: COG0608

COG function: function code L; Single-stranded DNA-specific exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recJ family [H]

Homologues:

Organism=Escherichia coli, GI1789259, Length=578, Percent_Identity=61.7647058823529, Blast_Score=750, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR003156
- InterPro:   IPR001667
- InterPro:   IPR004610 [H]

Pfam domain/function: PF01368 DHH; PF02272 DHHA1 [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 63722; Mature: 63722

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEVKRRTVPSSTQLPDSIPPILQRIYAARGITSSSQLERGAKFLLSFQSMHGISKAVDI
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LVEAIQKQTRIIVVGDFDADGATSSALSVMAFRMMGSQNVDYLVPNRFEDGYGLSPDVVD
HHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCHHHHH
QAKEMGAEIIMTVDNGVSSIDGVLKAKEYGMQVVVTDHHLPGAHLPLADAIVNPNLEACA
HHHHCCCEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHCCCCCHHHC
FPSKSLAGVGVAFYLMLAIRARLREINWFEKQNIAIPNLADLLDLVALGTVADLVALDEN
CCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHEECCC
NRILVHQGIQRIRAGKCRPGIQALIEVANRVPSRIVASDFGFALGPRINAAGRLDDMSFG
CEEEHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCHHHC
VELLMSNNIHAARRMAAELDSLNQTRKEIEQGMKEEAMAICERLEFGAQSELPFGLVLFQ
HHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEE
RDWHQGVIGILASRIKEKYHRPVIAFADGGDGFIKGSCRSIAGFHMRDALDLIDTRNPGL
CCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEECCHHHHHHHHHHHHHHHHCCCCCCE
ILKFGGHAMAAGLTIKEADYKKFSQAFDDVVRESVEEEALKGIILSDGALTPEEFTLGTA
EEEECCCEEECCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHCCCHH
EIIRAGGPWGQAFPEPIFDGEFKVLNQRLVGEKHLKLMLEPIHRDFPTNKMVDAIAFNID
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHCCCCCCHHHHHHHEEEE
VRRWPDASVQKVKLAYRLDINEFRGNQTLQLMVENIEPL
ECCCCCCCHHHEEEEEEECHHHHCCCCEEEHEECCCCCC
>Mature Secondary Structure
MIEVKRRTVPSSTQLPDSIPPILQRIYAARGITSSSQLERGAKFLLSFQSMHGISKAVDI
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LVEAIQKQTRIIVVGDFDADGATSSALSVMAFRMMGSQNVDYLVPNRFEDGYGLSPDVVD
HHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCHHHHH
QAKEMGAEIIMTVDNGVSSIDGVLKAKEYGMQVVVTDHHLPGAHLPLADAIVNPNLEACA
HHHHCCCEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHCCCCCHHHC
FPSKSLAGVGVAFYLMLAIRARLREINWFEKQNIAIPNLADLLDLVALGTVADLVALDEN
CCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHEECCC
NRILVHQGIQRIRAGKCRPGIQALIEVANRVPSRIVASDFGFALGPRINAAGRLDDMSFG
CEEEHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCHHHC
VELLMSNNIHAARRMAAELDSLNQTRKEIEQGMKEEAMAICERLEFGAQSELPFGLVLFQ
HHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEE
RDWHQGVIGILASRIKEKYHRPVIAFADGGDGFIKGSCRSIAGFHMRDALDLIDTRNPGL
CCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEECCHHHHHHHHHHHHHHHHCCCCCCE
ILKFGGHAMAAGLTIKEADYKKFSQAFDDVVRESVEEEALKGIILSDGALTPEEFTLGTA
EEEECCCEEECCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHCCCHH
EIIRAGGPWGQAFPEPIFDGEFKVLNQRLVGEKHLKLMLEPIHRDFPTNKMVDAIAFNID
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHCCCCCCHHHHHHHEEEE
VRRWPDASVQKVKLAYRLDINEFRGNQTLQLMVENIEPL
ECCCCCCCHHHEEEEEEECHHHHCCCCEEEHEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1987126; 9278503; 2649886 [H]