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The map label for this gene is pgk [H]

Identifier: 59711049

GI number: 59711049

Start: 470388

End: 471551

Strand: Direct

Name: pgk [H]

Synonym: VF_0442

Alternate gene names: 59711049

Gene position: 470388-471551 (Clockwise)

Preceding gene: 59711048

Following gene: 59711050

Centisome position: 16.23

GC content: 43.81

Gene sequence:

>1164_bases
ATGTCTGTAATCAAGATGACTGACCTGGAACTTGCAGGTAAACGCGTATTTATCCGTGCTGACCTAAACGTACCAGTTAA
AGATGGTAAAGTAACTTCTGATGCACGTATCCTTGCATCTCTACCAACTATCAAGCTTTGCTTGGAAGCTGGCGCTAAAG
TAATGGTTACTTCTCACCTAGGTCGTCCAACGGAAGGCGAGTACAACGAAGAGTTCTCTCTAGCTCCTGTAGTTAACTAC
TTGAATGACGCACTAGATTGTGACGTTAAGCTAGCAAAAGATTACCTAGACGGTCTTGAACTGAACGCTGGTGAATTAGT
TGTTCTTGAAAACGTTCGCTTCAACAAAGGCGAGAAGAAGAACGAAGAAGAGCTTTCTAAGAAATACGCTGCACTATGTG
ACATCTTCGTAATGGATGCATTTGGTACTGCTCACCGTGCACAAGCATCTACTCACGGTGTTGGCATGAACGCTCCTGTA
GCGTGTGCTGGTCCACTACTAGCTGCAGAACTTGAAGCACTTGGTAAAGCAATGTCTAACCCTGAGCGTCCACTAGTTGC
TATCGTTGGTGGTTCAAAAGTATCTACTAAGCTAACAGTTCTTGAGTCACTATCTAAAATCGCTGACCAACTTGTTGTTG
GTGGTGGTATCGCGAATACATTCATCGCTGCTGAAGGTCACAACGTAGGTAAATCACTATACGAAGCAGACCTAGTTGAA
ACAGCTCAAAAACTAATGAAAGAGTGTGCAATTCCAGTTGCTACTGATGTTGCATGTGCGAAAGCATTTGACGAAAACGC
TGAAGCTGAAATCAAACACGTTTCTGAAGTTCAAGATGACGACATGATTTTCGACCTTGGTCCAGATTCAACAGCTGCAC
TTGCTGAAATCATCGGCAATGCGAAAACAATTCTTTGGAATGGTCCAGTTGGCGTATTCGAATTCAAAAACTTCGAAGCG
GGTACAGCGGGTATCTCTAAAGCAATCGCTGAATCTGCAGGTTTCTCTGTAGCAGGTGGTGGTGATACACTAGCAGCTAT
CGACAAGTTCGGTATTAAAGCTGACGTATCTTACATCTCTACAGGTGGTGGTGCATTCCTTGAATTCGTTGAAGGTAAAG
TACTTCCTGCAGTAGCAATGCTTGAAGAGCGTGCTAAAGCATAA

Upstream 100 bases:

>100_bases
ACTGCATCCCTATTTACTGAATAATGCAGTAAGAATTTAATCGGTTTGGCAATGACGCCGAGCTTGAAAACTTTTTATTA
TTTAATTGAGAGGACAAAAC

Downstream 100 bases:

>100_bases
TTATTTAAGAGCGGGAATCTAATCATTCCCGCTTTTTACGTTTGTTGATGGCAAGGTTTATTGATACAATACCGACAGTT
GTGAGCAAACGTTTGCAAAA

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MSVIKMTDLELAGKRVFIRADLNVPVKDGKVTSDARILASLPTIKLCLEAGAKVMVTSHLGRPTEGEYNEEFSLAPVVNY
LNDALDCDVKLAKDYLDGLELNAGELVVLENVRFNKGEKKNEEELSKKYAALCDIFVMDAFGTAHRAQASTHGVGMNAPV
ACAGPLLAAELEALGKAMSNPERPLVAIVGGSKVSTKLTVLESLSKIADQLVVGGGIANTFIAAEGHNVGKSLYEADLVE
TAQKLMKECAIPVATDVACAKAFDENAEAEIKHVSEVQDDDMIFDLGPDSTAALAEIIGNAKTILWNGPVGVFEFKNFEA
GTAGISKAIAESAGFSVAGGGDTLAAIDKFGIKADVSYISTGGGAFLEFVEGKVLPAVAMLEERAKA

Sequences:

>Translated_387_residues
MSVIKMTDLELAGKRVFIRADLNVPVKDGKVTSDARILASLPTIKLCLEAGAKVMVTSHLGRPTEGEYNEEFSLAPVVNY
LNDALDCDVKLAKDYLDGLELNAGELVVLENVRFNKGEKKNEEELSKKYAALCDIFVMDAFGTAHRAQASTHGVGMNAPV
ACAGPLLAAELEALGKAMSNPERPLVAIVGGSKVSTKLTVLESLSKIADQLVVGGGIANTFIAAEGHNVGKSLYEADLVE
TAQKLMKECAIPVATDVACAKAFDENAEAEIKHVSEVQDDDMIFDLGPDSTAALAEIIGNAKTILWNGPVGVFEFKNFEA
GTAGISKAIAESAGFSVAGGGDTLAAIDKFGIKADVSYISTGGGAFLEFVEGKVLPAVAMLEERAKA
>Mature_386_residues
SVIKMTDLELAGKRVFIRADLNVPVKDGKVTSDARILASLPTIKLCLEAGAKVMVTSHLGRPTEGEYNEEFSLAPVVNYL
NDALDCDVKLAKDYLDGLELNAGELVVLENVRFNKGEKKNEEELSKKYAALCDIFVMDAFGTAHRAQASTHGVGMNAPVA
CAGPLLAAELEALGKAMSNPERPLVAIVGGSKVSTKLTVLESLSKIADQLVVGGGIANTFIAAEGHNVGKSLYEADLVET
AQKLMKECAIPVATDVACAKAFDENAEAEIKHVSEVQDDDMIFDLGPDSTAALAEIIGNAKTILWNGPVGVFEFKNFEAG
TAGISKAIAESAGFSVAGGGDTLAAIDKFGIKADVSYISTGGGAFLEFVEGKVLPAVAMLEERAKA

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI4505763, Length=413, Percent_Identity=38.498789346247, Blast_Score=250, Evalue=1e-66,
Organism=Homo sapiens, GI31543397, Length=413, Percent_Identity=38.0145278450363, Blast_Score=249, Evalue=2e-66,
Organism=Escherichia coli, GI1789294, Length=386, Percent_Identity=76.9430051813471, Blast_Score=597, Evalue=1e-172,
Organism=Caenorhabditis elegans, GI17508823, Length=418, Percent_Identity=39.9521531100478, Blast_Score=261, Evalue=6e-70,
Organism=Saccharomyces cerevisiae, GI6319857, Length=419, Percent_Identity=40.3341288782816, Blast_Score=267, Evalue=2e-72,
Organism=Drosophila melanogaster, GI17136394, Length=415, Percent_Identity=39.7590361445783, Blast_Score=253, Evalue=2e-67,
Organism=Drosophila melanogaster, GI24581203, Length=416, Percent_Identity=37.9807692307692, Blast_Score=239, Evalue=3e-63,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 40654; Mature: 40523

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVIKMTDLELAGKRVFIRADLNVPVKDGKVTSDARILASLPTIKLCLEAGAKVMVTSHL
CCEEEECCCEECCCEEEEEECCCCCCCCCCCCCCCHHHHHCCHHHHHHHCCCEEEEEECC
GRPTEGEYNEEFSLAPVVNYLNDALDCDVKLAKDYLDGLELNAGELVVLENVRFNKGEKK
CCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCC
NEEELSKKYAALCDIFVMDAFGTAHRAQASTHGVGMNAPVACAGPLLAAELEALGKAMSN
CHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHCC
PERPLVAIVGGSKVSTKLTVLESLSKIADQLVVGGGIANTFIAAEGHNVGKSLYEADLVE
CCCCEEEEECCCCHHHHHHHHHHHHHHHHHHEECCCHHHHEEEECCCHHHHHHHHHHHHH
TAQKLMKECAIPVATDVACAKAFDENAEAEIKHVSEVQDDDMIFDLGPDSTAALAEIIGN
HHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHCC
AKTILWNGPVGVFEFKNFEAGTAGISKAIAESAGFSVAGGGDTLAAIDKFGIKADVSYIS
CEEEEECCCCCEEEECCCCCCHHHHHHHHHHHCCCEEECCCCHHHHHHHCCCEEECCEEE
TGGGAFLEFVEGKVLPAVAMLEERAKA
CCCHHHHHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SVIKMTDLELAGKRVFIRADLNVPVKDGKVTSDARILASLPTIKLCLEAGAKVMVTSHL
CEEEECCCEECCCEEEEEECCCCCCCCCCCCCCCHHHHHCCHHHHHHHCCCEEEEEECC
GRPTEGEYNEEFSLAPVVNYLNDALDCDVKLAKDYLDGLELNAGELVVLENVRFNKGEKK
CCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCC
NEEELSKKYAALCDIFVMDAFGTAHRAQASTHGVGMNAPVACAGPLLAAELEALGKAMSN
CHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHCC
PERPLVAIVGGSKVSTKLTVLESLSKIADQLVVGGGIANTFIAAEGHNVGKSLYEADLVE
CCCCEEEEECCCCHHHHHHHHHHHHHHHHHHEECCCHHHHEEEECCCHHHHHHHHHHHHH
TAQKLMKECAIPVATDVACAKAFDENAEAEIKHVSEVQDDDMIFDLGPDSTAALAEIIGN
HHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHCC
AKTILWNGPVGVFEFKNFEAGTAGISKAIAESAGFSVAGGGDTLAAIDKFGIKADVSYIS
CEEEEECCCCCEEEECCCCCCHHHHHHHHHHHCCCEEECCCCHHHHHHHCCCEEECCEEE
TGGGAFLEFVEGKVLPAVAMLEERAKA
CCCHHHHHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA