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The map label for this gene is yrbE [H]

Identifier: 59711002

GI number: 59711002

Start: 427861

End: 428655

Strand: Direct

Name: yrbE [H]

Synonym: VF_0395

Alternate gene names: 59711002

Gene position: 427861-428655 (Clockwise)

Preceding gene: 59711001

Following gene: 59711003

Centisome position: 14.77

GC content: 42.01

Gene sequence:

>795_bases
ATGAGTAATTTTTCACTTGTCTCCTCAATAGAACAGATAGGACGACGAGCTTTATCGGTTTGTGAGGCCTTTGGTCGTGC
AACCTTTATGTTGCTTGGCGCGATATTTGCGAAACCTCAACCAATAAAAATGTTTCCTTTGTTGCTAAAGCAACTCTATT
CCGTAGGTGTTTTATCATTAGTGATTATTGTGGTTTCAGGGCTCTTTATTGGCATGGTAATCAGCTTGCAAGGTTATATT
ATTTTAGTTGATTACGGGGCTGAAACCAGTTTAGGGCAGCTTGTTTCCTTATCATTACTTAGAGAGTTAGGGCCTGTGGT
AACAGCGCTATTGTTTGCGGGACGCGCAGGCTCCGCATTAACCGCTGAAATTGGCTTAATGAAAACCACCGAACAAATTT
CAAGTATGGAGATGATGGCTGTAGACCCGTTGCGTCAAATTATCGCACCTCGTTTATGGGCAGGGATCATTTCGATGCCA
GTTCTCGCTGTCATCTTTATGGCTGTGGGTATTTGGGGAGGACAAATTGTCGGAGTTGAATGGAAAGGGATTGATTACGG
TAGTTTTTGGTCGGTAATGCAATCATCGGTTGATGTGCGATATGACATTATAAATAGCTTAATTAAATGTGTGGTGTTTG
CGTTTATTGTTACATGGATTGCTCTGTTTAATGGTTATGATGCAACGCCGACTTCTGAAGGTATTTCATTGGCAACGACA
CGTACAGTAGTGCACTCTTCGTTGGCGGTATTAGGTATTGATTTTGTGCTTACGGCACTGATGTTTGGGAACTAA

Upstream 100 bases:

>100_bases
AATCCCGATCCTCAAGTTCGACAATTTTTGGATGGTGAATCAGATGGACCAGTACCATTTCGTTTTCCGGCTAACACATT
AAATGAGGATCTTTTCTCTT

Downstream 100 bases:

>100_bases
ATTATGCAACAGACACGTAAAGTAGAATTTTTTGTTGGCCTTTTTGTATTAGCAGGAATGGCCGCTCTACTGATGTTATT
ACTTAAGGTTGCGGATGTTC

Product: toluene ABC transporter membrane protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MSNFSLVSSIEQIGRRALSVCEAFGRATFMLLGAIFAKPQPIKMFPLLLKQLYSVGVLSLVIIVVSGLFIGMVISLQGYI
ILVDYGAETSLGQLVSLSLLRELGPVVTALLFAGRAGSALTAEIGLMKTTEQISSMEMMAVDPLRQIIAPRLWAGIISMP
VLAVIFMAVGIWGGQIVGVEWKGIDYGSFWSVMQSSVDVRYDIINSLIKCVVFAFIVTWIALFNGYDATPTSEGISLATT
RTVVHSSLAVLGIDFVLTALMFGN

Sequences:

>Translated_264_residues
MSNFSLVSSIEQIGRRALSVCEAFGRATFMLLGAIFAKPQPIKMFPLLLKQLYSVGVLSLVIIVVSGLFIGMVISLQGYI
ILVDYGAETSLGQLVSLSLLRELGPVVTALLFAGRAGSALTAEIGLMKTTEQISSMEMMAVDPLRQIIAPRLWAGIISMP
VLAVIFMAVGIWGGQIVGVEWKGIDYGSFWSVMQSSVDVRYDIINSLIKCVVFAFIVTWIALFNGYDATPTSEGISLATT
RTVVHSSLAVLGIDFVLTALMFGN
>Mature_263_residues
SNFSLVSSIEQIGRRALSVCEAFGRATFMLLGAIFAKPQPIKMFPLLLKQLYSVGVLSLVIIVVSGLFIGMVISLQGYII
LVDYGAETSLGQLVSLSLLRELGPVVTALLFAGRAGSALTAEIGLMKTTEQISSMEMMAVDPLRQIIAPRLWAGIISMPV
LAVIFMAVGIWGGQIVGVEWKGIDYGSFWSVMQSSVDVRYDIINSLIKCVVFAFIVTWIALFNGYDATPTSEGISLATTR
TVVHSSLAVLGIDFVLTALMFGN

Specific function: Part of the ABC transporter complex mlaFEDB that actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner memb

COG id: COG0767

COG function: function code Q; ABC-type transport system involved in resistance to organic solvents, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaE permease family [H]

Homologues:

Organism=Escherichia coli, GI1789585, Length=259, Percent_Identity=70.2702702702703, Blast_Score=373, Evalue=1e-105,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003453 [H]

Pfam domain/function: PF02405 DUF140 [H]

EC number: NA

Molecular weight: Translated: 28425; Mature: 28294

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNFSLVSSIEQIGRRALSVCEAFGRATFMLLGAIFAKPQPIKMFPLLLKQLYSVGVLSL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
VIIVVSGLFIGMVISLQGYIILVDYGAETSLGQLVSLSLLRELGPVVTALLFAGRAGSAL
HHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
TAEIGLMKTTEQISSMEMMAVDPLRQIIAPRLWAGIISMPVLAVIFMAVGIWGGQIVGVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
WKGIDYGSFWSVMQSSVDVRYDIINSLIKCVVFAFIVTWIALFNGYDATPTSEGISLATT
ECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHH
RTVVHSSLAVLGIDFVLTALMFGN
HHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SNFSLVSSIEQIGRRALSVCEAFGRATFMLLGAIFAKPQPIKMFPLLLKQLYSVGVLSL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
VIIVVSGLFIGMVISLQGYIILVDYGAETSLGQLVSLSLLRELGPVVTALLFAGRAGSAL
HHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
TAEIGLMKTTEQISSMEMMAVDPLRQIIAPRLWAGIISMPVLAVIFMAVGIWGGQIVGVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
WKGIDYGSFWSVMQSSVDVRYDIINSLIKCVVFAFIVTWIALFNGYDATPTSEGISLATT
ECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHH
RTVVHSSLAVLGIDFVLTALMFGN
HHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]