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The map label for this gene is mshE [H]

Identifier: 59710969

GI number: 59710969

Start: 394181

End: 395905

Strand: Direct

Name: mshE [H]

Synonym: VF_0362

Alternate gene names: 59710969

Gene position: 394181-395905 (Clockwise)

Preceding gene: 59710968

Following gene: 59710970

Centisome position: 13.6

GC content: 40.0

Gene sequence:

>1725_bases
ATGCGTCTTAAGTTAAGAAAAAGACTTGGTGATCTATTAGTCGAAGAAAACATTGTTACTGAAGAGCAAATCGAACAGGC
TCTTTCGACTCAAAAGAAAACAGGGCGAAAGTTAGGGGCAACATTAATTCAGTTAGGTTTCCTGAGTGAAAAACAAATGT
TGACGTTTTTATCTCAGCAGTTGGGTGTCCCTTTAGTTGATTTGAACCGTGCTGAAATTGATGCAGCAGCCGTGCAATTG
TTGCCTGAAGTACATGCTCGTCGCTTACGTGCATTAGTTCTTAGTCAGCAAGGTAATACGATTCGAGTAGCAATGAGTGA
TCCTGCTGATCTGTCTGCTCAAGAAGCGTTGTTTTCACAGCTTCAGCAATATCAGGTAGAAATGGTTATTGCTTCTGAAA
GACAGCTCGTTGATAGCTTTGACCGTTTCTATCGTAGAACCAAAGAAATCGCTTCATTTGCAGAAAAATTGCATTCAGAG
CATCAAGTTAACGACGATTTTAATTTTGGTATATCAGATAACGATAATGAAGAAGTTACCGTTGTTAAGCTGATTAACTC
ATTATTCGAAGATGCGATTCAAGTTGGTGCTTCAGATATACATATTGAACCGGATGAAAGAGTCCTGCGTTTACGTCAAC
GTATTGATGGGGTATTACACGAAACATTATTAAATGAAGTTAATATCGCTTCAGCACTGGTTTTACGTTTAAAACTGATG
GCGAATTTGGATATTTCTGAAAAACGTTTGCCACAAGATGGACGATTTAACATTAAATCTCGTGGTCAATCTGTAGATAT
TCGTTTATCGACAATGCCTGTTCAATATGGTGAATCGGTTGTTATGCGTTTATTGAATCAGACATCTGGCGTTCAAGAAC
TAGAACAATCTGGATTACCTGAGCATTTACTTACTCGTCTGCGTAAACAACTCCGTCGTCCACATGGCATGATCTTAGTT
ACGGGGCCTACAGGTTCTGGTAAAACGACAACATTGTATGGTGCTTTAAGTGAATTAAATCAGCCAAGCAAGAAAATCAT
TACAGCAGAAGACCCGGTTGAATACCGTTTACCTCGTGTTAATCAGGTACAAGTAAATAGCAAAATTGATTTAAGCTTTT
CAAAAATTTTAAGAACATTCTTGCGTCAAGACCCGGATATTATCCTTATTGGTGAGATGCGTGATCAAGAAACCGTGGAA
ATTGGTTTACGTGCCGCTCTAACGGGTCACTTAGTGTTATCTACATTACATACGAATGACTCGGTTGATAGTGCATTACG
TATGATGGATATGGGAGCGCCAGGTTATCTGGTTGCTAGTGCAGTTCGTGCGGTATTAGCGCAGCGTCTAGTTAAAAAAG
TGTGTCCAGAGTGCTCTGCTCCTGATGAGTTAGATGAAGCGAGAAAGCAGTGGGTACAAAGCCGTTTTCCAAATCAAGTT
GGAGTAACTTTCCAACGAGGAAATGGATGTCAAAGTTGTAATTTGACAGGCTACCGTGGCCGTATTGGTGTGTTTGAATT
ACTTGAATTAGATCAGCCAATGATGGATGCATTAAGAGCAAATGATGCGGTTGGTTTTTCACAAAAGGCACGTCAAAACC
CTGATTATAAGCCATTGCTTGCTTCTGCAATGGAGCTTGCGATGAGTGGTGTGATTAGCTTGGATGAAGTGATGGTATTA
GGTGAAGGAGATAATGCATCTTCTCATCAACCAATATTGATGTAG

Upstream 100 bases:

>100_bases
CATACCAGACAGCATTAATTAAGCCGGGTTTATCTCGCTCGTCTCAAGCGTTTGTTCGTGATCGCATTAAATTATTAGCA
ACAATGGAAGGAATTGAGTA

Downstream 100 bases:

>100_bases
GCATAAATCATGGCAATTTTTAAATACAGTGGAAGAAACCTTCGAGGTGAGTCAGTTTCTGGTTCAATTGAAGCGACATC
TCAAGAATCTGCTGCTGAAC

Product: MSHA biogenesis protein MshE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 574; Mature: 574

Protein sequence:

>574_residues
MRLKLRKRLGDLLVEENIVTEEQIEQALSTQKKTGRKLGATLIQLGFLSEKQMLTFLSQQLGVPLVDLNRAEIDAAAVQL
LPEVHARRLRALVLSQQGNTIRVAMSDPADLSAQEALFSQLQQYQVEMVIASERQLVDSFDRFYRRTKEIASFAEKLHSE
HQVNDDFNFGISDNDNEEVTVVKLINSLFEDAIQVGASDIHIEPDERVLRLRQRIDGVLHETLLNEVNIASALVLRLKLM
ANLDISEKRLPQDGRFNIKSRGQSVDIRLSTMPVQYGESVVMRLLNQTSGVQELEQSGLPEHLLTRLRKQLRRPHGMILV
TGPTGSGKTTTLYGALSELNQPSKKIITAEDPVEYRLPRVNQVQVNSKIDLSFSKILRTFLRQDPDIILIGEMRDQETVE
IGLRAALTGHLVLSTLHTNDSVDSALRMMDMGAPGYLVASAVRAVLAQRLVKKVCPECSAPDELDEARKQWVQSRFPNQV
GVTFQRGNGCQSCNLTGYRGRIGVFELLELDQPMMDALRANDAVGFSQKARQNPDYKPLLASAMELAMSGVISLDEVMVL
GEGDNASSHQPILM

Sequences:

>Translated_574_residues
MRLKLRKRLGDLLVEENIVTEEQIEQALSTQKKTGRKLGATLIQLGFLSEKQMLTFLSQQLGVPLVDLNRAEIDAAAVQL
LPEVHARRLRALVLSQQGNTIRVAMSDPADLSAQEALFSQLQQYQVEMVIASERQLVDSFDRFYRRTKEIASFAEKLHSE
HQVNDDFNFGISDNDNEEVTVVKLINSLFEDAIQVGASDIHIEPDERVLRLRQRIDGVLHETLLNEVNIASALVLRLKLM
ANLDISEKRLPQDGRFNIKSRGQSVDIRLSTMPVQYGESVVMRLLNQTSGVQELEQSGLPEHLLTRLRKQLRRPHGMILV
TGPTGSGKTTTLYGALSELNQPSKKIITAEDPVEYRLPRVNQVQVNSKIDLSFSKILRTFLRQDPDIILIGEMRDQETVE
IGLRAALTGHLVLSTLHTNDSVDSALRMMDMGAPGYLVASAVRAVLAQRLVKKVCPECSAPDELDEARKQWVQSRFPNQV
GVTFQRGNGCQSCNLTGYRGRIGVFELLELDQPMMDALRANDAVGFSQKARQNPDYKPLLASAMELAMSGVISLDEVMVL
GEGDNASSHQPILM
>Mature_574_residues
MRLKLRKRLGDLLVEENIVTEEQIEQALSTQKKTGRKLGATLIQLGFLSEKQMLTFLSQQLGVPLVDLNRAEIDAAAVQL
LPEVHARRLRALVLSQQGNTIRVAMSDPADLSAQEALFSQLQQYQVEMVIASERQLVDSFDRFYRRTKEIASFAEKLHSE
HQVNDDFNFGISDNDNEEVTVVKLINSLFEDAIQVGASDIHIEPDERVLRLRQRIDGVLHETLLNEVNIASALVLRLKLM
ANLDISEKRLPQDGRFNIKSRGQSVDIRLSTMPVQYGESVVMRLLNQTSGVQELEQSGLPEHLLTRLRKQLRRPHGMILV
TGPTGSGKTTTLYGALSELNQPSKKIITAEDPVEYRLPRVNQVQVNSKIDLSFSKILRTFLRQDPDIILIGEMRDQETVE
IGLRAALTGHLVLSTLHTNDSVDSALRMMDMGAPGYLVASAVRAVLAQRLVKKVCPECSAPDELDEARKQWVQSRFPNQV
GVTFQRGNGCQSCNLTGYRGRIGVFELLELDQPMMDALRANDAVGFSQKARQNPDYKPLLASAMELAMSGVISLDEVMVL
GEGDNASSHQPILM

Specific function: Involved in the translocation of the type IV pilin [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=387, Percent_Identity=42.6356589147287, Blast_Score=324, Evalue=1e-89,
Organism=Escherichia coli, GI1786296, Length=442, Percent_Identity=36.8778280542986, Blast_Score=283, Evalue=2e-77,
Organism=Escherichia coli, GI87082188, Length=195, Percent_Identity=42.0512820512821, Blast_Score=120, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013374
- InterPro:   IPR007831
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 64106; Mature: 64106

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLKLRKRLGDLLVEENIVTEEQIEQALSTQKKTGRKLGATLIQLGFLSEKQMLTFLSQQ
CCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LGVPLVDLNRAEIDAAAVQLLPEVHARRLRALVLSQQGNTIRVAMSDPADLSAQEALFSQ
HCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHH
LQQYQVEMVIASERQLVDSFDRFYRRTKEIASFAEKLHSEHQVNDDFNFGISDNDNEEVT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCH
VVKLINSLFEDAIQVGASDIHIEPDERVLRLRQRIDGVLHETLLNEVNIASALVLRLKLM
HHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ANLDISEKRLPQDGRFNIKSRGQSVDIRLSTMPVQYGESVVMRLLNQTSGVQELEQSGLP
HCCCCCHHHCCCCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHCCCHHHHHHCCCC
EHLLTRLRKQLRRPHGMILVTGPTGSGKTTTLYGALSELNQPSKKIITAEDPVEYRLPRV
HHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCCCCEEECCCCHHHCCCCC
NQVQVNSKIDLSFSKILRTFLRQDPDIILIGEMRDQETVEIGLRAALTGHLVLSTLHTND
CEEEECCEECCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHEECCCCC
SVDSALRMMDMGAPGYLVASAVRAVLAQRLVKKVCPECSAPDELDEARKQWVQSRFPNQV
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHH
GVTFQRGNGCQSCNLTGYRGRIGVFELLELDQPMMDALRANDAVGFSQKARQNPDYKPLL
CEEEECCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHCCCCCCCCHHHCCCCCCHHHH
ASAMELAMSGVISLDEVMVLGEGDNASSHQPILM
HHHHHHHHHCCCCHHHEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MRLKLRKRLGDLLVEENIVTEEQIEQALSTQKKTGRKLGATLIQLGFLSEKQMLTFLSQQ
CCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LGVPLVDLNRAEIDAAAVQLLPEVHARRLRALVLSQQGNTIRVAMSDPADLSAQEALFSQ
HCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHH
LQQYQVEMVIASERQLVDSFDRFYRRTKEIASFAEKLHSEHQVNDDFNFGISDNDNEEVT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCH
VVKLINSLFEDAIQVGASDIHIEPDERVLRLRQRIDGVLHETLLNEVNIASALVLRLKLM
HHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ANLDISEKRLPQDGRFNIKSRGQSVDIRLSTMPVQYGESVVMRLLNQTSGVQELEQSGLP
HCCCCCHHHCCCCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHCCCHHHHHHCCCC
EHLLTRLRKQLRRPHGMILVTGPTGSGKTTTLYGALSELNQPSKKIITAEDPVEYRLPRV
HHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCCCCEEECCCCHHHCCCCC
NQVQVNSKIDLSFSKILRTFLRQDPDIILIGEMRDQETVEIGLRAALTGHLVLSTLHTND
CEEEECCEECCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHEECCCCC
SVDSALRMMDMGAPGYLVASAVRAVLAQRLVKKVCPECSAPDELDEARKQWVQSRFPNQV
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHH
GVTFQRGNGCQSCNLTGYRGRIGVFELLELDQPMMDALRANDAVGFSQKARQNPDYKPLL
CEEEECCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHCCCCCCCCHHHCCCCCCHHHH
ASAMELAMSGVISLDEVMVLGEGDNASSHQPILM
HHHHHHHHHCCCCHHHEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8820654 [H]