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The map label for this gene is mshG [H]

Identifier: 59710970

GI number: 59710970

Start: 395915

End: 397141

Strand: Direct

Name: mshG [H]

Synonym: VF_0363

Alternate gene names: 59710970

Gene position: 395915-397141 (Clockwise)

Preceding gene: 59710969

Following gene: 59710971

Centisome position: 13.66

GC content: 39.85

Gene sequence:

>1227_bases
ATGGCAATTTTTAAATACAGTGGAAGAAACCTTCGAGGTGAGTCAGTTTCTGGTTCAATTGAAGCGACATCTCAAGAATC
TGCTGCTGAACAGTTGATGAATGATGGAGTGATTCCTACTGGAATAAAAGCCAATAAATTTGGTGGAACATCTCGCTCCA
TGGACTGGAAAAAATGGTTGCAGCCAAGCATTCCTTTAGAGGTTTTGGTTATTTTTTGTCGTCAAATGTACAGCTTAACT
AAAGCGGGTGTGCCTTTACTGCGAGCAATGAATGGCTTAGCTCAGAACAGTAATAACAAATTATTAAAAACAACATTGGA
AGAGGTGACTGCTGATTTAACTAACGGGCGAAGTTTGTCTGTATCTATGCAGCAACACCCACAAGTGTTTAGCTCTTTGT
TTGTTTCAATGATCCATGTTGGTGAGAATACCGGTCGATTGGATCAATCTCTCATGCAATTGGCTAATTACTACGAACAA
GAGATGGAGACCCGTAAGCGAATTAAGTCAGCAATGCGTTATCCCGTATTTGTTTTAACTTTTATTTTGCTTGCGATGTT
TGTACTAAATATCAAAGTGATCCCTCAGTTTGCAGGGATGTTTGCTCGTTTTGGTGTGGAATTACCATTGCCAACTCGAA
TACTTATCGCAACATCGAGTTTCTTTGTTAATTATTGGCATTTATTAATTGGCGCAATGACTGGCGCGATGTTTGCTTTT
CAAGCTTGGATTAGCCGAGCTGAAGGCCGAGAAAAATGGGATAAATTTCGATTACGTTTACCGATTGTTGGTGGAATTGT
CACTCGAGCTCAAATGTCTCGTTTCTCAAGAACATTTGCATTGATGTTGAAGTCGGGGGTTCCATTAAATCAATCCCTTG
CACTATCAGCAGAGTCTCTAGGGAATAAATATTTAGAGAACCGATTACTAGAAATGAAAGCTGGCATTGAATCGGGTACC
AGTATGTCTGCGACAGCGGCACAAAGTGGAATCTTTACTCCTTTGGTACAACAGATGATTGCGGTTGGCGAAGAGACGGG
TCAAATAGACGATTTACTATTGGAAGTCTCTGATTTTTATGATCGTGAAGTGGATTATGATTTGAAGACGTTAACTGCAC
GAATTGAACCTATTCTATTGGTCATTGTGTCGGGGATGGTATTGATTTTAGCATTAGGTATTTTCTTACCGATGTGGAAT
ATGTTGGATGTAGTAAAGGGAACCTAA

Upstream 100 bases:

>100_bases
GCTTGCGATGAGTGGTGTGATTAGCTTGGATGAAGTGATGGTATTAGGTGAAGGAGATAATGCATCTTCTCATCAACCAA
TATTGATGTAGGCATAAATC

Downstream 100 bases:

>100_bases
TTATGTCAGCAACCCGGATTTTATGGGGCGCGCTTGTCATTATTTTATTGGCTGTGATTTTTATTAATTGGGAAAAAATC
GAACCCGAAGTAGAAGATAC

Product: MSHA biogenesis protein MshG

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 408; Mature: 407

Protein sequence:

>408_residues
MAIFKYSGRNLRGESVSGSIEATSQESAAEQLMNDGVIPTGIKANKFGGTSRSMDWKKWLQPSIPLEVLVIFCRQMYSLT
KAGVPLLRAMNGLAQNSNNKLLKTTLEEVTADLTNGRSLSVSMQQHPQVFSSLFVSMIHVGENTGRLDQSLMQLANYYEQ
EMETRKRIKSAMRYPVFVLTFILLAMFVLNIKVIPQFAGMFARFGVELPLPTRILIATSSFFVNYWHLLIGAMTGAMFAF
QAWISRAEGREKWDKFRLRLPIVGGIVTRAQMSRFSRTFALMLKSGVPLNQSLALSAESLGNKYLENRLLEMKAGIESGT
SMSATAAQSGIFTPLVQQMIAVGEETGQIDDLLLEVSDFYDREVDYDLKTLTARIEPILLVIVSGMVLILALGIFLPMWN
MLDVVKGT

Sequences:

>Translated_408_residues
MAIFKYSGRNLRGESVSGSIEATSQESAAEQLMNDGVIPTGIKANKFGGTSRSMDWKKWLQPSIPLEVLVIFCRQMYSLT
KAGVPLLRAMNGLAQNSNNKLLKTTLEEVTADLTNGRSLSVSMQQHPQVFSSLFVSMIHVGENTGRLDQSLMQLANYYEQ
EMETRKRIKSAMRYPVFVLTFILLAMFVLNIKVIPQFAGMFARFGVELPLPTRILIATSSFFVNYWHLLIGAMTGAMFAF
QAWISRAEGREKWDKFRLRLPIVGGIVTRAQMSRFSRTFALMLKSGVPLNQSLALSAESLGNKYLENRLLEMKAGIESGT
SMSATAAQSGIFTPLVQQMIAVGEETGQIDDLLLEVSDFYDREVDYDLKTLTARIEPILLVIVSGMVLILALGIFLPMWN
MLDVVKGT
>Mature_407_residues
AIFKYSGRNLRGESVSGSIEATSQESAAEQLMNDGVIPTGIKANKFGGTSRSMDWKKWLQPSIPLEVLVIFCRQMYSLTK
AGVPLLRAMNGLAQNSNNKLLKTTLEEVTADLTNGRSLSVSMQQHPQVFSSLFVSMIHVGENTGRLDQSLMQLANYYEQE
METRKRIKSAMRYPVFVLTFILLAMFVLNIKVIPQFAGMFARFGVELPLPTRILIATSSFFVNYWHLLIGAMTGAMFAFQ
AWISRAEGREKWDKFRLRLPIVGGIVTRAQMSRFSRTFALMLKSGVPLNQSLALSAESLGNKYLENRLLEMKAGIESGTS
MSATAAQSGIFTPLVQQMIAVGEETGQIDDLLLEVSDFYDREVDYDLKTLTARIEPILLVIVSGMVLILALGIFLPMWNM
LDVVKGT

Specific function: Involved in the translocation of the type IV pilin [H]

COG id: COG1459

COG function: function code NU; Type II secretory pathway, component PulF

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP F family [H]

Homologues:

Organism=Escherichia coli, GI1789724, Length=398, Percent_Identity=26.6331658291457, Blast_Score=182, Evalue=5e-47,
Organism=Escherichia coli, GI1786295, Length=340, Percent_Identity=25.8823529411765, Blast_Score=124, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003004
- InterPro:   IPR018076
- InterPro:   IPR001992 [H]

Pfam domain/function: PF00482 GSPII_F [H]

EC number: NA

Molecular weight: Translated: 45443; Mature: 45312

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIFKYSGRNLRGESVSGSIEATSQESAAEQLMNDGVIPTGIKANKFGGTSRSMDWKKWL
CCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHC
QPSIPLEVLVIFCRQMYSLTKAGVPLLRAMNGLAQNSNNKLLKTTLEEVTADLTNGRSLS
CCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEE
VSMQQHPQVFSSLFVSMIHVGENTGRLDQSLMQLANYYEQEMETRKRIKSAMRYPVFVLT
EEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
FILLAMFVLNIKVIPQFAGMFARFGVELPLPTRILIATSSFFVNYWHLLIGAMTGAMFAF
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHH
QAWISRAEGREKWDKFRLRLPIVGGIVTRAQMSRFSRTFALMLKSGVPLNQSLALSAESL
HHHHHHHCCHHHHHHHEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
GNKYLENRLLEMKAGIESGTSMSATAAQSGIFTPLVQQMIAVGEETGQIDDLLLEVSDFY
HHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
DREVDYDLKTLTARIEPILLVIVSGMVLILALGIFLPMWNMLDVVKGT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AIFKYSGRNLRGESVSGSIEATSQESAAEQLMNDGVIPTGIKANKFGGTSRSMDWKKWL
CEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHC
QPSIPLEVLVIFCRQMYSLTKAGVPLLRAMNGLAQNSNNKLLKTTLEEVTADLTNGRSLS
CCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEE
VSMQQHPQVFSSLFVSMIHVGENTGRLDQSLMQLANYYEQEMETRKRIKSAMRYPVFVLT
EEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
FILLAMFVLNIKVIPQFAGMFARFGVELPLPTRILIATSSFFVNYWHLLIGAMTGAMFAF
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHH
QAWISRAEGREKWDKFRLRLPIVGGIVTRAQMSRFSRTFALMLKSGVPLNQSLALSAESL
HHHHHHHCCHHHHHHHEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
GNKYLENRLLEMKAGIESGTSMSATAAQSGIFTPLVQQMIAVGEETGQIDDLLLEVSDFY
HHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
DREVDYDLKTLTARIEPILLVIVSGMVLILALGIFLPMWNMLDVVKGT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8820654 [H]