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The map label for this gene is mshL [H]

Identifier: 59710966

GI number: 59710966

Start: 390550

End: 392190

Strand: Direct

Name: mshL [H]

Synonym: VF_0359

Alternate gene names: 59710966

Gene position: 390550-392190 (Clockwise)

Preceding gene: 59710965

Following gene: 59710967

Centisome position: 13.48

GC content: 39.85

Gene sequence:

>1641_bases
ATGAAAGTACGTAATGTCATCATTGGTTTCATGGTTGCCTCTTTAACAGGTTGCTCAATGGGTCATCGTGACCCTGTTGA
AATTAAACAGACCTTAGATAAATCCATTAATGAAAATGGGGGGCTGCTACTTGCTGATTTACCCGCTTCAGTACAAGCTG
ATTTAATGCCTGAGCTAAACACGCCTCATGTTGAAGTTGAAGAGCCTGTGAAACGTTTCAGAGTCAGAGCAAATGGGGTA
GGTGCAAAAGCATTTTTTACGAGTTTAGTGAAAGGAACGGAATACAGTGTCGTTATTCATCCTCAGGTTTCTGGTCGTAT
TACGATGAACTTGAGTGATGTGACCATGGATGATGTTCTGTCATCGGTTGAAGACTTATATGGGTACGATATCGTTAAAG
CAGGAAAAGTATTACAAGTTTATCCAGCGACGATTCGTACAGAGACTATTCCTGTTGATTATTTACAGCTTACTCGTAAA
GGACGTTCATTAACGACGATTACGACAGGGTCGATTACCAACTCGGACAATAATTCATCAAATAACAATAACTCAAGTTC
TAAATCAAACAATAATTCATCGTCGTCGTCATCTTCTGCTACCGGTGGAACAGAGATTGAAACAACGAGTGAGAGCGATT
TTTGGAAAGAGTTAGAAACGGCTATTTCTGTGATGATAGGAAATGGAGCGGATCGAAGCGTTGTTGTATCTCCTCAAGCG
AGTGTTGTTACTATTACTGCCTCTCCTGATGAATTACGTAAAGTTAAACAATTTTTAGGAACCTCTCATAAGCGATTACA
ACGTCAAGTGATCCTAGAGGCTAAATTACTTGAAGTCACATTAAGTGATGGTTATCAGCAAGGTATTAATTGGTCGAACC
TAACAGCATCATTAGGTGGTACAGATGTTGTCTTTGGGCAGACTTCTGGCCTTGTTCAAGGTGCATTACCTGGAATGGAT
ACCATTGGAACATTGTTAGGTGGGCAAACAAACATTACGGTTTCAGATGGTAACTTCCAAGCTGTATTAAGTTTTATGGA
TACACAAGGTGACTTAAATGTACTTTCAAGCCCGAGAGTGACTGCTGCAAATAACCAAAAAGCGGTGATTAAAGTCGGTC
GTGACGAGTACTACGTAACTGAAGTATCAAGTGTTACTGGTAGTGGTGACAACTCTAATGCTTCTCCAGAAGTAACATTA
ACGCCTTTCTTCTCAGGTATTTCTTTAGATGTGACACCTCAAATCGATGATAAGGGTAATGTTATGTTACATGTTCACCC
ATCAGTTATTGATGTGGAGAACGAGGTAAAAGAGATTGATTTAGGTGATGTTGGTGGCGTACTGAAATTACCATTAGCAA
AGAGCTCAATTCGTGAATCAGATTCTGTGATCCGAGCGAAAAGTGGTGATGTGGTCGTTATTGGTGGTTTGATGAAATCA
CAAACGGTTGACCAAGTATCGAAAGTACCATTACTAGGTGATGTTCCTGCATTAGGGCATTTATTTAGAAATGTCAGTCA
GCTAACTCAAAAAACGGAATTGGTTATTTTATTAAAGCCAACGGTTGTCGGTGTAGATACTTGGCAAGAAGAATTAGAAC
GTTCTCGTGACTTATTACAAGAATGGTTTCCAGACGAGTAA

Upstream 100 bases:

>100_bases
TATTAACAATTGGTGAAGAAAGCGTTTCGATTGGTCGTGCGGGTAAGCAATGGCAACTTTCTCTATTTCCACAAAATATT
AAAAATTAAGGTTTGCAGTA

Downstream 100 bases:

>100_bases
TCAAAAACTGGCATGTATGAAGCTCACTTTGGGTTAAAGCAGTTACCATTTACACTGACACCCAATACCTCACTGTTTCA
TGGCTTATCGCCTCATTATG

Product: MSHA biogenesis protein MshL

Products: NA

Alternate protein names: Pectic enzymes secretion protein outD [H]

Number of amino acids: Translated: 546; Mature: 546

Protein sequence:

>546_residues
MKVRNVIIGFMVASLTGCSMGHRDPVEIKQTLDKSINENGGLLLADLPASVQADLMPELNTPHVEVEEPVKRFRVRANGV
GAKAFFTSLVKGTEYSVVIHPQVSGRITMNLSDVTMDDVLSSVEDLYGYDIVKAGKVLQVYPATIRTETIPVDYLQLTRK
GRSLTTITTGSITNSDNNSSNNNNSSSKSNNNSSSSSSSATGGTEIETTSESDFWKELETAISVMIGNGADRSVVVSPQA
SVVTITASPDELRKVKQFLGTSHKRLQRQVILEAKLLEVTLSDGYQQGINWSNLTASLGGTDVVFGQTSGLVQGALPGMD
TIGTLLGGQTNITVSDGNFQAVLSFMDTQGDLNVLSSPRVTAANNQKAVIKVGRDEYYVTEVSSVTGSGDNSNASPEVTL
TPFFSGISLDVTPQIDDKGNVMLHVHPSVIDVENEVKEIDLGDVGGVLKLPLAKSSIRESDSVIRAKSGDVVVIGGLMKS
QTVDQVSKVPLLGDVPALGHLFRNVSQLTQKTELVILLKPTVVGVDTWQEELERSRDLLQEWFPDE

Sequences:

>Translated_546_residues
MKVRNVIIGFMVASLTGCSMGHRDPVEIKQTLDKSINENGGLLLADLPASVQADLMPELNTPHVEVEEPVKRFRVRANGV
GAKAFFTSLVKGTEYSVVIHPQVSGRITMNLSDVTMDDVLSSVEDLYGYDIVKAGKVLQVYPATIRTETIPVDYLQLTRK
GRSLTTITTGSITNSDNNSSNNNNSSSKSNNNSSSSSSSATGGTEIETTSESDFWKELETAISVMIGNGADRSVVVSPQA
SVVTITASPDELRKVKQFLGTSHKRLQRQVILEAKLLEVTLSDGYQQGINWSNLTASLGGTDVVFGQTSGLVQGALPGMD
TIGTLLGGQTNITVSDGNFQAVLSFMDTQGDLNVLSSPRVTAANNQKAVIKVGRDEYYVTEVSSVTGSGDNSNASPEVTL
TPFFSGISLDVTPQIDDKGNVMLHVHPSVIDVENEVKEIDLGDVGGVLKLPLAKSSIRESDSVIRAKSGDVVVIGGLMKS
QTVDQVSKVPLLGDVPALGHLFRNVSQLTQKTELVILLKPTVVGVDTWQEELERSRDLLQEWFPDE
>Mature_546_residues
MKVRNVIIGFMVASLTGCSMGHRDPVEIKQTLDKSINENGGLLLADLPASVQADLMPELNTPHVEVEEPVKRFRVRANGV
GAKAFFTSLVKGTEYSVVIHPQVSGRITMNLSDVTMDDVLSSVEDLYGYDIVKAGKVLQVYPATIRTETIPVDYLQLTRK
GRSLTTITTGSITNSDNNSSNNNNSSSKSNNNSSSSSSSATGGTEIETTSESDFWKELETAISVMIGNGADRSVVVSPQA
SVVTITASPDELRKVKQFLGTSHKRLQRQVILEAKLLEVTLSDGYQQGINWSNLTASLGGTDVVFGQTSGLVQGALPGMD
TIGTLLGGQTNITVSDGNFQAVLSFMDTQGDLNVLSSPRVTAANNQKAVIKVGRDEYYVTEVSSVTGSGDNSNASPEVTL
TPFFSGISLDVTPQIDDKGNVMLHVHPSVIDVENEVKEIDLGDVGGVLKLPLAKSSIRESDSVIRAKSGDVVVIGGLMKS
QTVDQVSKVPLLGDVPALGHLFRNVSQLTQKTELVILLKPTVVGVDTWQEELERSRDLLQEWFPDE

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of the multiple pectic enzymes [H]

COG id: COG1450

COG function: function code NU; Type II secretory pathway, component PulD

Gene ontology:

Cell location: Cell outer membrane (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP D family [H]

Homologues:

Organism=Escherichia coli, GI87082242, Length=322, Percent_Identity=26.3975155279503, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1789793, Length=463, Percent_Identity=22.4622030237581, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001775
- InterPro:   IPR005644
- InterPro:   IPR004846
- InterPro:   IPR013356
- InterPro:   IPR004845 [H]

Pfam domain/function: PF00263 Secretin; PF03958 Secretin_N [H]

EC number: NA

Molecular weight: Translated: 58598; Mature: 58598

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVRNVIIGFMVASLTGCSMGHRDPVEIKQTLDKSINENGGLLLADLPASVQADLMPELN
CCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCHHHCCCCCC
TPHVEVEEPVKRFRVRANGVGAKAFFTSLVKGTEYSVVIHPQVSGRITMNLSDVTMDDVL
CCCEEHHHHHHHHEECCCCCCHHHHHHHHHCCCCEEEEEECCCCCEEEEEECCCCHHHHH
SSVEDLYGYDIVKAGKVLQVYPATIRTETIPVDYLQLTRKGRSLTTITTGSITNSDNNSS
HHHHHHHCCCCCCCCCEEEEECCEEEECCCCHHHHHHHCCCCEEEEEEECCEECCCCCCC
NNNNSSSKSNNNSSSSSSSATGGTEIETTSESDFWKELETAISVMIGNGADRSVVVSPQA
CCCCCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHEEEECCCCCCEEEECCCC
SVVTITASPDELRKVKQFLGTSHKRLQRQVILEAKLLEVTLSDGYQQGINWSNLTASLGG
CEEEEECCHHHHHHHHHHHCCHHHHHHHHHHHHHEEEEEEECCCHHHCCCCCCEEEECCC
TDVVFGQTSGLVQGALPGMDTIGTLLGGQTNITVSDGNFQAVLSFMDTQGDLNVLSSPRV
CEEEEECCCCCHHCCCCCHHHHHHHHCCCEEEEEECCCHHHHHHHHCCCCCCEECCCCCE
TAANNQKAVIKVGRDEYYVTEVSSVTGSGDNSNASPEVTLTPFFSGISLDVTPQIDDKGN
EECCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCCEEEEEEEECCEEEEECCCCCCCCC
VMLHVHPSVIDVENEVKEIDLGDVGGVLKLPLAKSSIRESDSVIRAKSGDVVVIGGLMKS
EEEEECCCEEECCCHHHCCCCCCCCCEEECCCCHHHHHCCCCEEEECCCCEEEECCCHHH
QTVDQVSKVPLLGDVPALGHLFRNVSQLTQKTELVILLKPTVVGVDTWQEELERSRDLLQ
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCEEECHHHHHHHHHHHHHHH
EWFPDE
HHCCCC
>Mature Secondary Structure
MKVRNVIIGFMVASLTGCSMGHRDPVEIKQTLDKSINENGGLLLADLPASVQADLMPELN
CCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCHHHCCCCCC
TPHVEVEEPVKRFRVRANGVGAKAFFTSLVKGTEYSVVIHPQVSGRITMNLSDVTMDDVL
CCCEEHHHHHHHHEECCCCCCHHHHHHHHHCCCCEEEEEECCCCCEEEEEECCCCHHHHH
SSVEDLYGYDIVKAGKVLQVYPATIRTETIPVDYLQLTRKGRSLTTITTGSITNSDNNSS
HHHHHHHCCCCCCCCCEEEEECCEEEECCCCHHHHHHHCCCCEEEEEEECCEECCCCCCC
NNNNSSSKSNNNSSSSSSSATGGTEIETTSESDFWKELETAISVMIGNGADRSVVVSPQA
CCCCCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHEEEECCCCCCEEEECCCC
SVVTITASPDELRKVKQFLGTSHKRLQRQVILEAKLLEVTLSDGYQQGINWSNLTASLGG
CEEEEECCHHHHHHHHHHHCCHHHHHHHHHHHHHEEEEEEECCCHHHCCCCCCEEEECCC
TDVVFGQTSGLVQGALPGMDTIGTLLGGQTNITVSDGNFQAVLSFMDTQGDLNVLSSPRV
CEEEEECCCCCHHCCCCCHHHHHHHHCCCEEEEEECCCHHHHHHHHCCCCCCEECCCCCE
TAANNQKAVIKVGRDEYYVTEVSSVTGSGDNSNASPEVTLTPFFSGISLDVTPQIDDKGN
EECCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCCEEEEEEEECCEEEEECCCCCCCCC
VMLHVHPSVIDVENEVKEIDLGDVGGVLKLPLAKSSIRESDSVIRAKSGDVVVIGGLMKS
EEEEECCCEEECCCHHHCCCCCCCCCEEECCCCHHHHHCCCCEEEECCCCEEEECCCHHH
QTVDQVSKVPLLGDVPALGHLFRNVSQLTQKTELVILLKPTVVGVDTWQEELERSRDLLQ
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCEEECHHHHHHHHHHHHHHH
EWFPDE
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1429461 [H]