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The map label for this gene is pucG [H]

Identifier: 59710946

GI number: 59710946

Start: 365309

End: 366421

Strand: Direct

Name: pucG [H]

Synonym: VF_0339

Alternate gene names: 59710946

Gene position: 365309-366421 (Clockwise)

Preceding gene: 59710944

Following gene: 172087658

Centisome position: 12.61

GC content: 38.9

Gene sequence:

>1113_bases
ATGCAAAGTTTTATACCACCGAAAAGAACCCTTATGGGACCTGGTCCTTCTGACATTTCTCCTCAAGTTTTACAAGCATT
AAGCCGTCCCACTATCGGTCACCTTGATCCATTATTTATTAAAATGATGGATGAAGTTAAAGAGCTACTCAAGTACGCAT
TTCAAACTAAAAATGACTTTACTATTGCAGTTTCAGCCCCAGGTAGTGCAGGGATGGAAACATGCTTTGTGAATTTAGTT
GAAGAAGGCGAGAAAGTACTCGTATGTCGTAATGGTGTATTTGGTGAACGAATGCGACAAAATGTAGAACGCTGTGGTGG
TATTCCTATTATTATCGATGATAAATGGGGTGAACCTGTTTCATTAGATAAAGTCGCGCAAGCATTGGAAGAAAACCCTG
ATATTAGCGTTGTTGCTTTTGTGCATGCGGAAACATCTACTGGAGTTCTTAGTGATGCAAAAGCGATTGCACAAATTGCG
AGACAATATAATTGTTTAACCATTGTGGATGCAGTGACTTCGTTAGGTGGTGTACCTTTGTTAGTTGATGAATGGCAGTT
AGATGCGGTTTATTCTGGTTCTCAAAAATGCTTATCTTGTACTCCTGGATTATCTCCTGTTACTTTTTCTGATAAAGCAG
TTGCTAAAATGAAATCGAGATCCTCGGTAGTACAAAGTTGGTTTTTAGATCAAAGTTTGGTTCTTGGGTATTGGAGTGGA
GAAGGAAAGCGCAGTTATCACCATACTGCACCAGTAAATAGTTTATATGCTTTGCATGAATCACTGTTAATGCTTAAACA
TGAAGGAATTGAGCAGGCGTGGCAGCGTCATGCTGATTATCACCAAGAGTTAAAACAAGGCCTTGAAGCGTTAGGGATTA
AGTTTATTGTTGATGAAGAATATCGTTTACCACAATTGAATGCCGTATATATACCTGTAGGTATTTCTGATACTCAAGTT
AGAGAGCAGTTGCTAGAGGAATATAATTTAGAGATCGGTGCTGGTTTAGGTGAACTTGCTGGTAAGGCATGGCGTATCGG
TCTTATGGGATACGCAGCAAAAAAAGAGAATGTCGCATTGTGCTTAAAAGCATTATCTGATGTTTTAAAGTAA

Upstream 100 bases:

>100_bases
GCTATTTTTCGTAATTAAGTCACTCTAATCAAATAGTCTAATTGAGTTTAATTTTTCTTATGTCTATTCTTTAATCACCT
CATATTGATAGGAGTTTATA

Downstream 100 bases:

>100_bases
ATATTAAATAAAACAGCCACTAAATCCCGTGGCTGTTTATGCTAATAGTTTTTAGTTAGTCATCTGTAATTTCTTATCGA
GTTGTACGGATTCAAATTTA

Product: serine--pyruvate aminotransferase

Products: 3-hydroxypyruvate; L-alanine

Alternate protein names: NA

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MQSFIPPKRTLMGPGPSDISPQVLQALSRPTIGHLDPLFIKMMDEVKELLKYAFQTKNDFTIAVSAPGSAGMETCFVNLV
EEGEKVLVCRNGVFGERMRQNVERCGGIPIIIDDKWGEPVSLDKVAQALEENPDISVVAFVHAETSTGVLSDAKAIAQIA
RQYNCLTIVDAVTSLGGVPLLVDEWQLDAVYSGSQKCLSCTPGLSPVTFSDKAVAKMKSRSSVVQSWFLDQSLVLGYWSG
EGKRSYHHTAPVNSLYALHESLLMLKHEGIEQAWQRHADYHQELKQGLEALGIKFIVDEEYRLPQLNAVYIPVGISDTQV
REQLLEEYNLEIGAGLGELAGKAWRIGLMGYAAKKENVALCLKALSDVLK

Sequences:

>Translated_370_residues
MQSFIPPKRTLMGPGPSDISPQVLQALSRPTIGHLDPLFIKMMDEVKELLKYAFQTKNDFTIAVSAPGSAGMETCFVNLV
EEGEKVLVCRNGVFGERMRQNVERCGGIPIIIDDKWGEPVSLDKVAQALEENPDISVVAFVHAETSTGVLSDAKAIAQIA
RQYNCLTIVDAVTSLGGVPLLVDEWQLDAVYSGSQKCLSCTPGLSPVTFSDKAVAKMKSRSSVVQSWFLDQSLVLGYWSG
EGKRSYHHTAPVNSLYALHESLLMLKHEGIEQAWQRHADYHQELKQGLEALGIKFIVDEEYRLPQLNAVYIPVGISDTQV
REQLLEEYNLEIGAGLGELAGKAWRIGLMGYAAKKENVALCLKALSDVLK
>Mature_370_residues
MQSFIPPKRTLMGPGPSDISPQVLQALSRPTIGHLDPLFIKMMDEVKELLKYAFQTKNDFTIAVSAPGSAGMETCFVNLV
EEGEKVLVCRNGVFGERMRQNVERCGGIPIIIDDKWGEPVSLDKVAQALEENPDISVVAFVHAETSTGVLSDAKAIAQIA
RQYNCLTIVDAVTSLGGVPLLVDEWQLDAVYSGSQKCLSCTPGLSPVTFSDKAVAKMKSRSSVVQSWFLDQSLVLGYWSG
EGKRSYHHTAPVNSLYALHESLLMLKHEGIEQAWQRHADYHQELKQGLEALGIKFIVDEEYRLPQLNAVYIPVGISDTQV
REQLLEEYNLEIGAGLGELAGKAWRIGLMGYAAKKENVALCLKALSDVLK

Specific function: Could encode ureidoglycolase or L-alanine:glyoxylate aminotransferase, or perhaps both activities [H]

COG id: COG0075

COG function: function code E; Serine-pyruvate aminotransferase/archaeal aspartate aminotransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI4557289, Length=366, Percent_Identity=37.1584699453552, Blast_Score=256, Evalue=2e-68,
Organism=Caenorhabditis elegans, GI17536281, Length=364, Percent_Identity=37.9120879120879, Blast_Score=255, Evalue=3e-68,
Organism=Saccharomyces cerevisiae, GI6321079, Length=389, Percent_Identity=28.2776349614396, Blast_Score=112, Evalue=1e-25,
Organism=Drosophila melanogaster, GI17530823, Length=368, Percent_Identity=37.5, Blast_Score=268, Evalue=5e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: 2.6.1.51

Molecular weight: Translated: 40677; Mature: 40677

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSFIPPKRTLMGPGPSDISPQVLQALSRPTIGHLDPLFIKMMDEVKELLKYAFQTKNDF
CCCCCCCCHHCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCE
TIAVSAPGSAGMETCFVNLVEEGEKVLVCRNGVFGERMRQNVERCGGIPIIIDDKWGEPV
EEEEECCCCCCHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEECCCCCCC
SLDKVAQALEENPDISVVAFVHAETSTGVLSDAKAIAQIARQYNCLTIVDAVTSLGGVPL
CHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEE
LVDEWQLDAVYSGSQKCLSCTPGLSPVTFSDKAVAKMKSRSSVVQSWFLDQSLVLGYWSG
EEECCEEEEEECCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECC
EGKRSYHHTAPVNSLYALHESLLMLKHEGIEQAWQRHADYHQELKQGLEALGIKFIVDEE
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCC
YRLPQLNAVYIPVGISDTQVREQLLEEYNLEIGAGLGELAGKAWRIGLMGYAAKKENVAL
CCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCHHHHCCCCEEEEHHHCCCCCCHHHH
CLKALSDVLK
HHHHHHHHHC
>Mature Secondary Structure
MQSFIPPKRTLMGPGPSDISPQVLQALSRPTIGHLDPLFIKMMDEVKELLKYAFQTKNDF
CCCCCCCCHHCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCE
TIAVSAPGSAGMETCFVNLVEEGEKVLVCRNGVFGERMRQNVERCGGIPIIIDDKWGEPV
EEEEECCCCCCHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEECCCCCCC
SLDKVAQALEENPDISVVAFVHAETSTGVLSDAKAIAQIARQYNCLTIVDAVTSLGGVPL
CHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEE
LVDEWQLDAVYSGSQKCLSCTPGLSPVTFSDKAVAKMKSRSSVVQSWFLDQSLVLGYWSG
EEECCEEEEEECCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECC
EGKRSYHHTAPVNSLYALHESLLMLKHEGIEQAWQRHADYHQELKQGLEALGIKFIVDEE
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCC
YRLPQLNAVYIPVGISDTQVREQLLEEYNLEIGAGLGELAGKAWRIGLMGYAAKKENVAL
CCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCHHHHCCCCEEEEHHHCCCCCCHHHH
CLKALSDVLK
HHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-serine; pyruvate

Specific reaction: L-serine + pyruvate = 3-hydroxypyruvate + L-alanine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11344136 [H]